2,472 research outputs found
A Practical Guide for X-Ray Diffraction Characterization of Ga(Al, In)N Alloys
Ga(In, Al)N alloys are used as an active layer or cladding layer in light
emitting diodes and laser diodes. x-ray diffraction is extensively used to
evaluate the crystalline quality, the chemical composition and the residual
strain in Ga(Al,In)N thin films, which directly determine the emission
wavelength and the device performance. Due to the minor mismatch in lattice
parameters between Ga(Al, In)N alloy and a GaN virtual substrate, x-ray
diffraction comes to a problem to separate the signal from Ga(Al,In)N alloy and
GaN. We give a detailed comparison on different diffraction planes. In order to
balance the intensity and peak separation between Ga(Al,In)N alloy and GaN,
(0004) and (1015) planes make the best choice for symmetric scan and asymmetric
scan, respectively.Comment: 9 pages, 5 figure
Effects of crystallinity and point defects on optoelectronic applications of β-Ga2O3 epilayers
This study evaluates the effect of crystallinity and point defects on time-dependent photoresponsivity and the cathodoluminescence (CL) properties of β-Ga2O3 epilayers. A synchrotron high-resolution X-ray technique was used to understand the crystalline structure of samples. Rutherford backscattering spectroscopy was used to determine the net chemical composition of the samples to examine the type and ratio of their possible point defects. The results show that in functional time-dependent photoresponsivity of photodetectors based on β-Ga2O3 epilayers, point defects contribution overcomes the contribution of crystallinity. However, the crystalline structure affects the intensities and emission regions of CL spectra more than point defects
Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
Background Enterocytozoon hepatopenaei (EHP) is a newly emerged microsporidian parasite that causes retarded shrimp growth in many countries. But there are no effective approaches to control this disease to date. The EHP could be an immune risk factor for increased dissemination of other diseases. Further, EHP infection involves the absence of obvious clinical signs and it is difficult to identify the pathogen through visual examination, increasing the risk of disease dissemination. It is urgent and necessary to develop a specific, rapid and sensitive EHP-infected shrimp diagnostic method to detect this parasite. In the present study, we developed and evaluated a rapid real-time loop-mediated isothermal amplification (real-time LAMP) for detection of EHP. Methods A rapid and efficient real-time LAMP method for the detection of EHP has been developed. Newly emerged EHP pathogens in China were collected and used as the sample, and three sets of specificity and sensitivity primers were designed. Three other aquatic pathogens were used as templates to test the specificity of the real-time LAMP assay. Also, we compared the real-time LAMP with the conventional LAMP by the serial dilutions of EHP DNA and their amplification curves. Application of real-time LAMP was carried out with clinical samples. Results Positive products were amplified only from EHP, but not from other tested species, EHP was detected from the clinical samples, suggesting a high specificity of this method. The final results of this assay were available within less than 45 min, and the initial amplification curve was observed at about 6 min. We found that the amplification with an exponential of sixfold dilutions of EHP DNA demonstrated a specific positive signal by the real-time LAMP, but not for the LAMP amplicons from the visual inspection. The real-time LAMP amplification curves demonstrated a higher slope than the conventional LAMP. Discussion In this study, pathogen virulence impacts have been increased in aquaculture and continuous observation was predominantly focused on EHP. The present study confirmed that the real-time LAMP assay is a promising and convenient method for the rapid identification of EHP in less time and cost. Its application greatly aids in the detection, surveillance, and prevention of EHP
Confront Holographic QCD with Regge Trajectories of vectors and axial-vectors
We derive the general 5-dimension metric structure of the system in
type II superstring theory, and demonstrate the physical meaning of the
parameters characterizing the 5-dimension metric structure of the
\textit{holographic} QCD model by relating them to the parameters describing
Regge trajectories. By matching the spectra of vector mesons with
deformed soft-wall model, we find that the spectra of vector mesons
can be described very well in the soft-wall model, i.e,
soft-wall model. We then investigate how well the soft-wall
model can describe the Regge trajectory of axial-vector mesons . We find
that the constant component of the 5-dimension mass square of axial-vector
mesons plays an efficient role to realize the chiral symmetry breaking in the
vacuum, and a small negative correction in the 5-dimension mass square is
helpful to realize the chiral symmetry restoration in high excitation states.Comment: 9 pages, 3 figure and 3 tables, one section adde
Regionalization by fuzzy expert system based approach optimized by genetic algorithm.
In recent years soft computing methods are being increasingly used to model complex hydrologic processes. These methods can simulate the real life processes without prior knowledge of the exact relationship between their components. The principal aim of this paper is perform hydrological regionalization based on soft computing concepts in the southern strip of the Caspian Sea basin, north of Iran. The basin with an area of 42,400 sq. km has been affected by severe floods in recent years that caused damages to human life and properties. Although some 61 hydrometric stations and 31 weather stations with 44 years of observed data (1961–2005) are operated in the study area, previous flood studies in this region have been hampered by insufficient and/or reliable observed rainfall-runoff records. In order to investigate the homogeneity (h) of catchments and overcome incompatibility that may occur on boundaries of cluster groups, a fuzzy expert system (FES) approach is used which incorporates physical and climatic characteristics, as well as flood seasonality and geographic location. Genetic algorithm (GA) was employed to adjust parameters of FES and optimize the system. In order to achieve the objective, a MATLAB programming code was developed which considers the heterogeneity criteria of less than 1 (H < 1) as the satisfying criteria. The adopted approach was found superior to the conventional hydrologic regionalization methods in the region because it employs greater number of homogeneity parameters and produces lower values of heterogeneity criteria
Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B
Salvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin α2β1. But, the signal cascades in platelets after SB binding are still not clear.In the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin α2β1 was predicted. To certify the predicted network, binding affinity of SB to integrin α2β1 was checked in vitro and ex vivo in platelets. Furthermore, the effects of SB on protein levels of hsp70, coronin-1B and intracellular levels of Ca²+ and reactive oxygen species (ROS) were checked with or without pre-treatment of platelets using antibody against integrin α2β1. Electron microscopy study confirmed that SB affected cytoskeleton structure of platelets.Integrin α2β1 might be one of the direct target proteins of SB in platelets. The signal cascades network of SB after binding with integrin α2β1 might include regulation of intracellular Ca²+ level, cytoskeleton-related proteins such as coronin-1B and cytoskeleton structure of platelets
A near IR imaging survey of intermediate and high-mass young stellar outflow candidates
We have carried out a near-infrared imaging survey of luminous young stellar
outflow candidates using the United Kingdom Infrared Telescope. Observations
were obtained in the broad band K (2.2 mu) and through narrow band filters at
the wavelengths of H_2 v=1--0 S(1) (2.1218 mu) and Br gamma (2.166 mu) lines.
Fifty regions were imaged with a field of view of 2.2 X 2.2 arcmin^2. Several
young embedded clusters are unveiled in our near-infrared images. 76% of the
objects exhibit H_2 emission and 50% or more of the objects exhibit aligned H_2
emission features suggesting collimated outflows, many of which are new
detections. These observations suggest that disk accretion is probably the
leading mechanism in the formation of stars, at least up to late O spectral
types. The young stellar objects responsible for many of these outflows are
positively identified in our images based on their locations with respect to
the outflow lobes, 2MASS colours and association with MSX, IRAS, millimetre and
radio sources. The close association of molecular outflows detected in CO with
the H_2 emission features produced by shock excitation by jets from the young
stellar objects suggests that the outflows from these objects are jet-driven.
Towards strong radio emitting sources, H_2 jets were either not detected or
were weak when detected, implying that most of the accretion happens in the
pre-UCHII phase; accretion and outflows are probably weak when the YSO has
advanced to its UCHII stage.Comment: 64 pages, 53 figures, Accepted for publication in the MNRA
The G9.62+0.19-F Hot Molecular Core - The infrared view on very young massive stars
(abridged) We present the results of an extensive infrared study of the
massive star-forming region G9.62+0.19. The data cover information from broad-
and narrow-band filters in the wavelength range from 1 to 19 micrometer and are
obtained with ESO's infrared cameras ISAAC and TIMMI2 and with SpectroCam-10
(Mt. Palomar). The high sensitivity and resolution provided by these facilities
revealed intriguing new details of this star-forming region and especially
about the embedded hot molecular core (HMC) - component F. We analyse the newly
found infrared sub-structure of four objects in this HMC region. While one of
these objects (F2) is probably a foreground field star, the nature of the
brightest object in the near-infrared there (F1) remains somewhat enigmatic.
Our new astrometry proves that this object is not coincident with the peak of
the molecular line emission of the HMC, but displaced by 1.7 arcsecs (nearly
10000 AU on a linear scale). We estimate this object to be an additional
embedded object with a dense dust shell. Very near the HMC location we find L'
band emission which strongly rises in flux towards longer wavelengths. We
presume that this emission (F4) arises from the envelope of the HMC which is
known to be associated with a molecular outflow roughly aligned along the line
of sight. Thus, the clearing effect of this outflow causes strong deviations
from spherical symmetry which might allow infrared emission from the HMC to
escape through the outflow cavities. This presents the first direct detection
of an HMC at a wavelength as short as 3.8 micron. At 11.7 and 18.75 micron, the
HMC counterpart F4 ultimately proves to be the most luminous IR source within
the G9.62+0.19-F region.Comment: 39 pages, 10 figures, accepted for publication in Astronomy &
Astrophysics. The paper contains several colour images. It is highly
advisable to go to the following website to get a high-resolution version of
this preprint:
http://www.tls-tautenburg.de/research/tls-research/papers/linz/G9.62.htm
Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass
Objective: To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction treatment and men visiting the subfertility clinics. Intervention(s): Human spermatozoa were allowed to penetrate through the cumulus oophorus and cell-depleted cumulus matrix in a capillary, and were treated with cumulus cell extract or hyaluronic acid. Main Outcome Measure(s): The morphology, acrosomal status, and motility of human spermatozoa were determined. Result(s): Fewer spermatozoa could penetrate the fresh cell-depleted matrix compared with intact cumulus oophorus. Spermatozoa that penetrated through the cumulus oophorus had higher percentages of normal morphology and acrosome reaction and had specific motility pattern. These effects were lost or reduced in the cell-depleted matrix that had been stored overnight. Hyaluronic acid, a main component of the cumulus matrix at concentration found in the cumulus oophorus, modulated sperm motility but did not affect spontaneous acrosome reaction. Cumulus cell extract did not affect sperm motility, but induced acrosome reaction. Conclusion(s): Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it. © 2009 American Society for Reproductive Medicine.postprin
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