CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
research
Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass
Authors
Bains
Baker
+60 more
Baker
Bastias
Camaioni
Chen
Chen
Cherr
Chian
Chiu
Chiu
Chiu
Chiu
Corselli
Cummins
Dandekar
de Lamirande
Dirnfeld
Fetterolf
Fulop
Gardner
Gardner
Gavella
Gerez
Goldberg
Hizaki
Hong
Hong
Hossain
Huszar
Huszar
Jenny Yin-Mei Tse
Jones
Kai-Fai Lee
Kornovski
Ling
Luconi
Mansour
Menkveld
Ng
O'Flaherty
Ohta
Pak-Chung Ho
Pereda
Philip Chi-Ngong Chiu
Ranganathan
Rodgers
Sabeur
Salustri
Shun-Jia Hong
Suarez
Sullivan
Sun
Talbot
Tanghe
Tatemoto
Tesarik
Tsuiki
William Shu-Biu Yeung
Yao
Zhuo
Zhuo
Publication date
1 January 2009
Publisher
'Elsevier BV'
Doi
Cite
Abstract
Objective: To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction treatment and men visiting the subfertility clinics. Intervention(s): Human spermatozoa were allowed to penetrate through the cumulus oophorus and cell-depleted cumulus matrix in a capillary, and were treated with cumulus cell extract or hyaluronic acid. Main Outcome Measure(s): The morphology, acrosomal status, and motility of human spermatozoa were determined. Result(s): Fewer spermatozoa could penetrate the fresh cell-depleted matrix compared with intact cumulus oophorus. Spermatozoa that penetrated through the cumulus oophorus had higher percentages of normal morphology and acrosome reaction and had specific motility pattern. These effects were lost or reduced in the cell-depleted matrix that had been stored overnight. Hyaluronic acid, a main component of the cumulus matrix at concentration found in the cumulus oophorus, modulated sperm motility but did not affect spontaneous acrosome reaction. Cumulus cell extract did not affect sperm motility, but induced acrosome reaction. Conclusion(s): Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it. © 2009 American Society for Reproductive Medicine.postprin
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
HKU Scholars Hub
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:hub.hku.hk:10722/125307
Last time updated on 01/06/2016
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
Last time updated on 03/09/2020