75 research outputs found
Intraoperative Management of Robotic-Assisted Versus Open Radical Prostatectomy
Robotic-assisted laparoscopic radical prostatectomy was found to be a shorter procedure characterized by minimal blood loss, reduced fluid requirements, and shorter hospital stay compared with traditional open procedures
Transition From Laparoscopic to Robotic Partial Nephrectomy: the Learning Curve for an Experienced Laparoscopic Surgeon
The transition from laparoscopic partial nephrectomy to robotic partial nephrectomy was found to be too rapid for an experienced laparoscopic surgeon
Risk Stratification and Early Oncologic Outcomes Following Robotic Prostatectomy
Results of this study suggest that robotic prostatectomy provides good cancer outcomes for clinically localized disease
Robotic Assisted Laparoscopic Prostatectomy Performed after Previous Suprapubic Prostatectomy
Operative management of prostate cancer in a patient who has undergone previous open suprapubic simple prostatectomy poses a unique surgical challenge. Herein, we describe a case of intermediate risk prostate cancer in a man who had undergone simple prostatectomy ten years prior to presentation. The patient was found to have Gleason 7 prostate cancer on MRI fusion biopsy of the prostate for elevated PSA and underwent an uncomplicated robot assisted laparoscopic radical prostatectomy
Lysophosphatidate Induces Chemo-Resistance by Releasing Breast Cancer Cells from Taxol-Induced Mitotic Arrest
Taxol is a microtubule stabilizing agent that arrests cells in mitosis leading to cell death. Taxol is widely used to treat breast cancer, but resistance occurs in 25-69% of patients and it is vital to understand how Taxol resistance develops to improve chemotherapy. The effects of chemotherapeutic agents are overcome by survival signals that cancer cells receive. We focused our studies on autotaxin, which is a secreted protein that increases tumor growth, aggressiveness, angiogenesis and metastasis. We discovered that autotaxin strongly antagonizes the Taxol-induced killing of breast cancer and melanoma cells by converting the abundant extra-cellular lipid, lysophosphatidylcholine, into lysophosphatidate. This lipid stimulates specific G-protein coupled receptors that activate survival signals.In this study we determined the basis of these antagonistic actions of lysophosphatidate towards Taxol-induced G2/M arrest and cell death using cultured breast cancer cells. Lysophosphatidate does not antagonize Taxol action in MCF-7 cells by increasing Taxol metabolism or its expulsion through multi-drug resistance transporters. Lysophosphatidate does not lower the percentage of cells accumulating in G2/M by decreasing exit from S-phase or selective stimulation of cell death in G2/M. Instead, LPA had an unexpected and remarkable action in enabling MCF-7 and MDA-MB-468 cells, which had been arrested in G2/M by Taxol, to normalize spindle structure and divide, thus avoiding cell death. This action involves displacement of Taxol from the tubulin polymer fraction, which based on inhibitor studies, depends on activation of LPA receptors and phosphatidylinositol 3-kinase.This work demonstrates a previously unknown consequence of lysophosphatidate action that explains why autotaxin and lysophosphatidate protect against Taxol-induced cell death and promote resistance to the action of this important therapeutic agent
Isolated communities of Epsilonproteobacteria in hydrothermal vent fluids of the Mariana Arc seamounts
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in FEMS Microbiology Ecology 73 (2010): 538-549, doi:10.1111/j.1574-6941.2010.00910.x.Low-temperature hydrothermal vent fluids represent access points to diverse microbial
communities living in oceanic crust. This study examined the distribution, relative abundance,
and diversity of Epsilonproteobacteria in 14 low-temperature vent fluids from 5 volcanically
active seamounts of the Mariana Arc using a 454 tag sequencing approach. Most vent fluids
were enriched in cell concentrations compared to background seawater, and quantitative PCR
results indicated all fluids were dominated by bacteria. Operational taxonomic unit (OTU)-based
statistical tools applied to 454 data show that all vents from the northern end of the Marian Arc
grouped together, to the exclusion of southern arc seamounts, which were as distinct from one
another as they were from northern seamounts. Statistical analysis also showed a significant
relationship between seamount and individual vent groupings, suggesting that community
membership may be linked to geographical isolation and not geochemical parameters. However,
while there may be large-scale geographic differences, distance is not the distinguishing factor in
microbial community composition. At the local scale, most vents host a distinct population of
Epsilonprotoebacteria, regardless of seamount location. This suggests there may be barriers to
exchange and dispersal for these vent endemic microorganisms at hydrothermal seamounts of the
Mariana Arc.This work was supported by a National Research
Council Research Associateship Award and L’Oréal USA Fellowship (J.A.H.), NASA
Astrobiology Institute Cooperative Agreement NNA04CC04A (M.L.S.), the Alfred P. Sloan
Foundation’s ICoMM field project, and the W. M. Keck Foundation. This publication is
[partially] funded by the Joint Institute for the Study of the Atmosphere and Ocean (JISAO)
under NOAA Cooperative Agreement No. NA17RJ1232, Contribution #1814
Broad targeting of resistance to apoptosis in cancer
Apoptosis or programmed cell death is natural way of removing aged cells from the body. Most of the anti-cancer therapies trigger apoptosis induction and related cell death networks to eliminate malignant cells. However, in cancer, de-regulated apoptotic signaling, particularly the activation of an anti-apoptotic systems, allows cancer cells to escape this program leading to uncontrolled proliferation resulting in tumor survival, therapeutic resistance and recurrence of cancer. This resistance is a complicated phenomenon that emanates from the interactions of various molecules and signaling pathways. In this comprehensive review we discuss the various factors contributing to apoptosis resistance in cancers. The key resistance targets that are discussed include (1) Bcl-2 and Mcl-1 proteins; (2) autophagy processes; (3) necrosis and necroptosis; (4) heat shock protein signaling; (5) the proteasome pathway; (6) epigenetic mechanisms; and (7) aberrant nuclear export signaling. The shortcomings of current therapeutic modalities are highlighted and a broad spectrum strategy using approaches including (a) gossypol; (b) epigallocatechin-3-gallate; (c) UMI-77 (d) triptolide and (e) selinexor that can be used to overcome cell death resistance is presented. This review provides a roadmap for the design of successful anti-cancer strategies that overcome resistance to apoptosis for better therapeutic outcome in patients with cancer
First records, rediscovery and compilation of deep-sea echinoderms in the middle and lower continental slope of the Mediterranean Sea
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