1,710 research outputs found
Transcriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weight
Indexación: Web of Science; PubMedBackground
Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood. Considering its economic importance, it is relevant to determine its genetic architecture and elucidate the mechanisms involved in its expression. To approach this issue, an RNA-Seq experiment based on Illumina platform was performed (14 libraries), including seedless segregants with contrasting phenotypes for berry weight at fruit setting (FST) and 6–8 mm berries (B68) phenological stages.
Results
A group of 526 differentially expressed (DE) genes were identified, by comparing seedless segregants with contrasting phenotypes for berry weight: 101 genes from the FST stage and 463 from the B68 stage. Also, we integrated differential expression, principal components analysis (PCA), correlations and network co-expression analyses to characterize the transcriptome profiling observed in segregants with contrasting phenotypes for berry weight. After this, 68 DE genes were selected as candidate genes, and seven candidate genes were validated by real time-PCR, confirming their expression profiles.
Conclusions
We have carried out the first transcriptome analysis focused on table grape seedless segregants with contrasting phenotypes for berry weight. Our findings contributed to the understanding of the mechanisms involved in berry weight determination. Also, this comparative transcriptome profiling revealed candidate genes for berry weight which could be evaluated as selection tools in table grape breeding programs.http://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0789-
Development and characterization of the readout system for POLARBEAR-2
POLARBEAR-2 is a next-generation receiver for precision measurements of the
polarization of the cosmic microwave background (Cosmic Microwave Background
(CMB)). Scheduled to deploy in early 2015, it will observe alongside the
existing POLARBEAR-1 receiver, on a new telescope in the Simons Array on Cerro
Toco in the Atacama desert of Chile. For increased sensitivity, it will feature
a larger area focal plane, with a total of 7,588 polarization sensitive
antenna-coupled Transition Edge Sensor (TES) bolometers, with a design
sensitivity of 4.1 uKrt(s). The focal plane will be cooled to 250 milliKelvin,
and the bolometers will be read-out with 40x frequency domain multiplexing,
with 36 optical bolometers on a single SQUID amplifier, along with 2 dark
bolometers and 2 calibration resistors. To increase the multiplexing factor
from 8x for POLARBEAR-1 to 40x for POLARBEAR-2 requires additional bandwidth
for SQUID readout and well-defined frequency channel spacing. Extending to
these higher frequencies requires new components and design for the LC filters
which define channel spacing. The LC filters are cold resonant circuits with an
inductor and capacitor in series with each bolometer, and stray inductance in
the wiring and equivalent series resistance from the capacitors can affect
bolometer operation. We present results from characterizing these new readout
components. Integration of the readout system is being done first on a small
scale, to ensure that the readout system does not affect bolometer sensitivity
or stability, and to validate the overall system before expansion into the full
receiver. We present the status of readout integration, and the initial results
and status of components for the full array.Comment: Presented at SPIE Astronomical Telescopes and Instrumentation 2014:
Millimeter, Submillimeter, and Far-Infrared Detectors and Instrumentation for
Astronomy VII. Published in Proceedings of SPIE Volume 915
Low-temperature magnetism in YbBiPt
Positive-muon (μ+) spin-relaxation experiments have been carried out in a pressed-powder sample of the low-carrier-density heavy-electron system YbBiPt. Spatially inhomogeneous and disordered static Yb magnetism is observed below ∼0.5 K, with a strongly reduced Yb moment of ∼0.1μB over ∼50% of the sample volume at T=0.06 K. Substantial μ+ spin-lattice relaxation, rarely observed in heavy-electron systems, suggests anomalously slow Yb spin fluctuations. Our data are reminiscent of μ+ behavior in spin glasses, and raise the question of whether the large low-temperature specific heat in YbBiPt is due in part to low-lying magnetic excitations. © 1992 The American Physical Society
Susceptibility weighted imaging: differentiating between calcification and hemosiderin
Search for direct stau production in events with two hadronic tau-leptons in root s=13 TeV pp collisions with the ATLAS detector
A search for the direct production of the supersymmetric partners ofτ-leptons (staus) in final stateswith two hadronically decayingτ-leptons is presented. The analysis uses a dataset of pp collisions corresponding to an integrated luminosity of139fb−1, recorded with the ATLAS detector at the LargeHadron Collider at a center-of-mass energy of 13 TeV. No significant deviation from the expected StandardModel background is observed. Limits are derived in scenarios of direct production of stau pairs with eachstau decaying into the stable lightest neutralino and oneτ-lepton in simplified models where the two staumass eigenstates are degenerate. Stau masses from 120 GeV to 390 GeV are excluded at 95% confidencelevel for a massless lightest neutralino
Search for chargino-neutralino production with mass splittings near the electroweak scale in three-lepton final states in √s=13 TeV pp collisions with the ATLAS detector
A search for supersymmetry through the pair production of electroweakinos with mass splittings near the electroweak scale and decaying via on-shell W and Z bosons is presented for a three-lepton final state. The analyzed proton-proton collision data taken at a center-of-mass energy of √s=13 TeV were collected between 2015 and 2018 by the ATLAS experiment at the Large Hadron Collider, corresponding to an integrated luminosity of 139 fb−1. A search, emulating the recursive jigsaw reconstruction technique with easily reproducible laboratory-frame variables, is performed. The two excesses observed in the 2015–2016 data recursive jigsaw analysis in the low-mass three-lepton phase space are reproduced. Results with the full data set are in agreement with the Standard Model expectations. They are interpreted to set exclusion limits at the 95% confidence level on simplified models of chargino-neutralino pair production for masses up to 345 GeV
Systematisation of spatial uncertainties for comparison between a MR and a CT-based radiotherapy workflow for prostate treatments
<p>Abstract</p> <p>Background</p> <p>In the present work we compared the spatial uncertainties associated with a MR-based workflow for external radiotherapy of prostate cancer to a standard CT-based workflow. The MR-based workflow relies on target definition and patient positioning based on MR imaging. A solution for patient transport between the MR scanner and the treatment units has been developed. For the CT-based workflow, the target is defined on a MR series but then transferred to a CT study through image registration before treatment planning, and a patient positioning using portal imaging and fiducial markers.</p> <p>Methods</p> <p>An "open bore" 1.5T MRI scanner, Siemens Espree, has been installed in the radiotherapy department in near proximity to a treatment unit to enable patient transport between the two installations, and hence use the MRI for patient positioning. The spatial uncertainty caused by the transport was added to the uncertainty originating from the target definition process, estimated through a review of the scientific literature. The uncertainty in the CT-based workflow was estimated through a literature review.</p> <p>Results</p> <p>The systematic uncertainties, affecting all treatment fractions, are reduced from 3-4 mm (1Sd) with a CT based workflow to 2-3 mm with a MR based workflow. The main contributing factor to this improvement is the exclusion of registration between MR and CT in the planning phase of the treatment.</p> <p>Conclusion</p> <p>Treatment planning directly on MR images reduce the spatial uncertainty for prostate treatments.</p
Experimental One-Way Quantum Computing
Standard quantum computation is based on sequences of unitary quantum logic
gates which process qubits. The one-way quantum computer proposed by
Raussendorf and Briegel is entirely different. It has changed our understanding
of the requirements for quantum computation and more generally how we think
about quantum physics. This new model requires qubits to be initialized in a
highly-entangled cluster state. From this point, the quantum computation
proceeds by a sequence of single-qubit measurements with classical feedforward
of their outcomes. Because of the essential role of measurement a one-way
quantum computer is irreversible. In the one-way quantum computer the order and
choices of measurements determine the algorithm computed. We have
experimentally realized four-qubit cluster states encoded into the polarization
state of four photons. We fully characterize the quantum state by implementing
the first experimental four-qubit quantum state tomography. Using this cluster
state we demonstrate the feasibility of one-way quantum computing through a
universal set of one- and two-qubit operations. Finally, our implementation of
Grover's search algorithm demonstrates that one-way quantum computation is
ideally suited for such tasks.Comment: 36 pages, 6 figures, 2 table
Recommended from our members
COVID-19, systemic crisis, and possible implications for the wild meat trade in sub-Saharan Africa
Wild animals play an integral and complex role in the economies and ecologies of many
countries across the globe, including those of West and Central Africa, the focus of this
policy perspective. The trade in wild meat, and its role in diets, have been brought into
focus as a consequence of discussions over the origins of COVID-19. As a result, there
have been calls for the closure of China’s “wet markets”; greater scrutiny of the wildlife
trade in general; and a spotlight has been placed on the potential risks posed by growing human populations and shrinking natural habitats for animal to human transmission of
zoonotic diseases. However, to date there has been little attention given to what the consequences of the COVID-19 economic shock may be for the wildlife trade; the people who
rely on it for their livelihoods; and the wildlife that is exploited. In this policy perspective,
we argue that the links between the COVID-19 pandemic, rural livelihoods and wildlife
are likely to be more complex, more nuanced, and more far-reaching, than is represented in
the literature to date. We develop a causal model that tracks the likely implications for the
wild meat trade of the systemic crisis triggered by COVID-19. We focus on the resulting
economic shockwave, as manifested in the collapse in global demand for commodities such
as oil, and international tourism services, and what this may mean for local African economies and livelihoods. We trace the shockwave through to the consequences for the use
of, and demand for, wild meats as households respond to these changes. We suggest that
understanding and predicting the complex dynamics of wild meat use requires increased
collaboration between environmental and resource economics and the ecological and conservation sciences
Modeling Atmospheric Emission for CMB Ground-based Observations
Atmosphere is one of the most important noise sources for ground-based cosmic microwave background (CMB) experiments. By increasing optical loading on the detectors, it amplifies their effective noise, while its fluctuations introduce spatial and temporal correlations between detected signals. We present a physically motivated 3D-model of the atmosphere total intensity emission in the millimeter and sub-millimeter wavelengths. We derive a new analytical estimate for the correlation between detectors time-ordered data as a function of the instrument and survey design, as well as several atmospheric parameters such as wind, relative humidity, temperature and turbulence characteristics. Using an original numerical computation, we examine the effect of each physical parameter on the correlations in the time series of a given experiment. We then use a parametric-likelihood approach to validate the modeling and estimate atmosphere parameters from the POLARBEAR-I project first season data set. We derive a new 1.0% upper limit on the linear polarization fraction of atmospheric emission. We also compare our results to previous studies and weather station measurements. The proposed model can be used for realistic simulations of future ground-based CMB observations
- …