Epidemiological aspects of facial trauma in a third level hospital in Mexico

Background: Facial trauma is common in early adulthood and may require complex surgery and even high mortality. Methods: Retrospective, cross-sectional study at the Hospital General de México during the period from January 2018 to December 2021. Patients with a diagnosis of fracture of the facial region, who had a complete clinical record, of any age, were included. Patients who did not comply with the treatment in the hospital unit, with incomplete clinical records, were excluded. Through non-probability sampling, a sample of 156 patients was formed. The descriptive data analysis was carried out by calculating frequencies and percentages for the qualitative variables. For the quantitative variables, measures of central tendency and dispersion were calculated according to the distribution of the variables (mean and standard deviation). for variables with normal distribution and median with interquartile range for variables with non-normal distribution). Results: 156 patients were included, the male sex predominated (89.7%), the age group from 21 to 30 years (35.3%), the injury mechanism of aggression by a third party (54.5%). Facial fractures occurred in the following descending order: orbit (64.7%), zygoma (41.7% n=65), mandibular (23.1% n=36), nasal (22.4% n=35), maxilla (21.8% n=34), NOE (4.5% n=7), Le Fort (3.8% n=6), and palate (3.8% n=6). Within orbital fractures, the most frequently affected region was the orbital floor (42.3%). The patients with orbital fracture were mostly men (88.1% versus 11.9%). The highest frequency of these fractures was between the ages of 21 and 30 (38.6%). Surgical treatment of fractures in general was established in 44.9%, performing open reduction and internal fixation of fractures in 41.0%. Conclusions: The most frequent facial fractures are: orbit, zygoma, and mandibular; they mainly affect the male sex in the second decade of life, they are produced mainly by aggressions to third parties. The management of facial fractures is predominantly surgical, through open reduction and internal fixation

Proteomics uncovers novel components of an interactive protein network supporting RNA export in trypanosomes

In trypanosomatids, transcription is polycistronic and all mRNAs are processed by trans-splicing, with export mediated by noncanonical mechanisms. Although mRNA export is central to gene regulation and expression, few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes, necessitating direct identification of protein components. We previously described conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII (previously Hel45), a core component of the exon junction complex (EJC). Here, we searched for protein interactors of both proteins using cryomilling and mass spectrometry. Significant overlap between TcSub2 and TceIF4AIII-interacting protein cohorts suggests that both proteins associate with similar machinery. We identified several interactions with conserved core components of the EJC and multiple additional complexes, together with proteins specific to trypanosomatids. Additional immunoisolations of kinetoplastid-specific proteins both validated and extended the superinteractome, which is capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events. We also suggest that only proteomics is powerful enough to uncover the high connectivity between multiple aspects of mRNA metabolism and to uncover kinetoplastid-specific components that create a unique amalgam to support trypanosome mRNA maturation

The OCCASO survey: presentation and radial velocities of 12 Milky Way open clusters

Open clusters (OCs) are crucial for studying the formation and evolution of the Galactic disc. However, the lack of a large number of OCs analysed homogeneously hampers the investigations about chemical patterns and the existence of Galactocentric radial and vertical gradients, or an age-metallicity relation. To overcome this, we have designed the Open Cluster Chemical Abundances from Spanish Observatories (OCCASO) survey. We aim to provide homogeneous radial velocities, physical parameters and individual chemical abundances of six or more red clump stars for a sample of 25 old and intermediate-age OCs visible from the Northern hemisphere. To do so, we use high-resolution spectroscopic facilities (R >= 62 000) available at Spanish observatories. We present the motivation, design and current status of the survey, together with the first data release of radial velocities for 77 stars in 12 OCs, which represents about 50 per cent of the survey. We include clusters never studied with high-resolution spectroscopy before (NGC 1907, NGC 6991, NGC 7762), and clusters in common with other large spectroscopic surveys like the Gaia-ESO Survey (NGC 6705) and Apache Point Observatory Galactic Evolution Experiment (NGC 2682 and NGC 6819). We perform internal comparisons between instruments to evaluate and correct internal systematics of the results, and compare our radial velocities with previous determinations in the literature, when available. Finally, radial velocities for each cluster are used to perform a preliminary kinematic study in relation with the Galactic disc

Comportamiento reproductivo del Cebú Cubano Bermejo de la Empresa Genética Rescate de Sanguily del municipio Camagüey

Records of 3,075 deliveries between the years 1981 - 2002 were used in the herd 22 of Cebu Bermejo of the Genetic Rescue Company of Sanguily, aged 28 to 256 months, with the objective of determining the influence of non-genetic factors : number of births (12), time (6), year of delivery (22) and sex of the offspring (2) and as genetic factor the stallions (35). For the statistical analysis a linear model was used by the method of Minimum Squares (SPSS, 2001), estimating the general means and their standard deviations for each trait studied. Reproductive traits period of service (PS) and labor-delivery interval (IPP) were significantly affected (P <0.01) for the time of delivery and the stallion for the number of services per gestation (S / G). The general averages and their standard deviations were: PS (287.6 ± 17.16 days); IPP (572.9 ± 36.18 days); DG (285.3 ± 3.28 days) and S / G (1.8 ± 1.04) inseminations performed).Se utilizaron los registros de 3 075 partos comprendidos entre los años 1981 – 2002 en el rebaño 22 de Cebú Bermejo de la Empresa Genética Rescate de Sanguily, con edades de 28 a 256 meses, con el objetivo de determinar la influencia de los factores no genéticos: número de partos (12), época (6), año del parto (22) y sexo de las crías (2) y como factor genético los sementales (35). Para el análisis estadístico se empleó un modelo lineal por el método de los Mínimos Cuadrados (SPSS, 2001), estimándose las medias generales y sus desviaciones estándar para cada rasgo estudiado. Los rasgos reproductivos periodo de servicio (PS) e intervalo parto–parto (IPP) fueron afectados significativamente (P< 0.01) para la época del parto y el semental para el número de servicios por gestación (S/G). Las medias generales y sus desviaciones estándar fueron: PS (287.6 ±17.16 días); IPP (572.9 ± 36.18 días); DG (285.3 ± 3.28 días) y S/G (1.8 ± 1.04) inseminaciones realizadas)

OCCASO - II. Physical parameters and Fe abundances of red clump stars in 18 open clusters

Open clusters have long been used to study the chemodynamical evolution of the Galactic disc. This requires a homogeneously analysed sample covering a wide range of ages and distances. In this paper, we present the Open Clusters Chemical Abundances from Spanish Observatories (OCCASO) second data release. This comprises a sample of high-resolution (R > 65 000) and high signal-to-noise spectra of 115 red clump stars in 18 open clusters. We derive atmospheric parameters (Teff, log g, ξ), and [Fe/H] abundances using two analysis techniques: equivalent widths and spectral synthesis. A detailed comparison and a critical review of the results of the two methods are made. Both methods are carefully tested between them, with the Gaia FGK benchmark stars, and with an extensive sample of literature values. We perform a membership study using radial velocities and the resulting abundances. Finally, we compare our results with a chemodynamical model of the Milky Way thin disc concluding that the oldest open clusters are consistent with the models only when dynamical effects are taken into account

The Open Cluster Chemical Abundances from Spanish Observatories survey (OCCASO)

We present the motivation, design and current status of the Open Cluster Chemical Abundances from Spanish Observatories survey (OCCASO). Using the high resolution spectroscopic facilities available at Spanish observatories, OCCASO will derive chemical abundances in a sample of 20 to 25 OCs older than 0.5 Gyr. This sample will be used to study in detail de formation and evolution of the Galactic disc using OCs as tracers. <P /

Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles

<div><p>Recent development of benzoxaborole-based chemistry gave rise to a collection of compounds with great potential in targeting diverse infectious diseases, including human African Trypanosomiasis (HAT), a devastating neglected tropical disease. However, further medicinal development is largely restricted by a lack of insight into mechanism of action (MoA) in pathogenic kinetoplastids. We adopted a multidisciplinary approach, combining a high-throughput forward genetic screen with functional group focused chemical biological, structural biology and biochemical analyses, to tackle the complex MoAs of benzoxaboroles in <i>Trypanosoma brucei</i>. We describe an oxidative enzymatic pathway composed of host semicarbazide-sensitive amine oxidase and a trypanosomal aldehyde dehydrogenase TbALDH3. Two sequential reactions through this pathway serve as the key underlying mechanism for activating a series of 4-aminomethylphenoxy-benzoxaboroles as potent trypanocides; the methylamine parental compounds as pro-drugs are transformed first into intermediate aldehyde metabolites, and further into the carboxylate metabolites as effective forms. Moreover, comparative biochemical and crystallographic analyses elucidated the catalytic specificity of TbALDH3 towards the benzaldehyde benzoxaborole metabolites as xenogeneic substrates. Overall, this work proposes a novel drug activation mechanism dependent on both host and parasite metabolism of primary amine containing molecules, which contributes a new perspective to our understanding of the benzoxaborole MoA, and could be further exploited to improve the therapeutic index of antimicrobial compounds.</p></div

Association of Candidate Gene Polymorphisms With Chronic Kidney Disease: Results of a Case-Control Analysis in the Nefrona Cohort

Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2, 445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionizationtime of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements