SERPINB3 delays glomerulonephritis and attenuates the lupus-like disease in lupus murine models by inducing a more tolerogenic immune phenotype

Objective: To explore the effects of SERPINB3 administration in murine lupus models with a focus on lupus-like nephritis. Methods: 40 NZB/W F1 mice were subdivided into 4 groups and intraperitoneally injected with recombinant SERPINB3 (7.5 \u3bcg/0.1 mL or 15 \u3bcg/0.1 mL) or PBS (0.1 mL) before (group 1 and 2) or after (group 3 and 4) the development of proteinuria ( 65100 mg/dl). Two additional mice groups were provided by including 20 MRL/lpr mice which were prophylactically injected with SERPINB3 (10 mice, group 5) or PBS (10 mice, group 6). Time of occurrence and levels of anti-dsDNA and anti-C1q antibodies, proteinuria and serum creatinine, overall- and proteinuria-free survival were assessed in mice followed up to natural death. Histological analysis was performed in kidneys of both lupus models. The Th17:Treg cell ratio was assessed by flow-cytometry in splenocytes of treated and untreated MRL/lpr mice. Statistical analysis was performed using non parametric tests and Kaplan-Meier curves, when indicated. Results: Autoantibody levels and proteinuria were significantly decreased and time of occurrence significantly delayed in SERPINB3-treated mice vs. controls. In agreement with these findings, proteinuria-free and overall survival were significantly improved in SERPINB3-treated groups vs. controls. Histological analysis demonstrated a lower prevalence of severe tubular lesions in kidneys of group 5 vs. group 6. SERPINB3-treated mice showed an overall trend toward a reduced prevalence of severe lesions in both strains. Th17:Treg ratio was significantly decreased in splenocytes of MRL/lpr mice treated with SERPINB3, compared to untreated control mice. Conclusions: SERPINB3 significantly improves disease course and delays the onset of severe glomerulonephritis in lupus-prone mice, possibly inducing a more tolerogenic immune phenotype

Hydrological assessment and monitoring of wetlands

The physical and chemical characteristics which favour wetland plant communities, primarily high soil water levels and anaerobic soil chemistry, are related directly to the hydrology/hydrogeology of the wetland and often its surrounding catchment. Appreciation and successful management of a wetland therefore almost always requires an understanding of its hydrological functioning, including the influences on hydrological functioning which often lie beyond the designated boundary of the sit

SOD1 Function and Its Implications for Amyotrophic Lateral Sclerosis Pathology: New and Renascent Themes

The canonical role of superoxide dismutase 1 (SOD1) is as an antioxidant enzyme protecting the cell from reactive oxygen species toxicity. SOD1 was also the first gene in which mutations were found to be causative for the neurodegenerative disease amyotrophic lateral sclerosis (ALS), more than 20 years ago. ALS is a relentless and incurable mid-life onset disease, which starts with a progressive paralysis and usually leads to death within 3 to 5 years of diagnosis; in the majority of cases, the intellect appears to remain intact while the motor system degenerates. It rapidly became clear that when mutated SOD1 takes on a toxic gain of function in ALS. However, this novel function remains unknown and many cellular systems have been implicated in disease. Now it seems that SOD1 may play a rather larger role in the cell than originally realized, including as a key modulator of glucose signaling (at least so far in yeast) and in RNA binding. Here, we consider some of the new findings for SOD1 in health and disease, which may shed light on how single amino acid changes at sites throughout this protein can cause devastating neurodegeneration in the mammalian motor system

Design of a polishing tool for collaborative robotics using minimum viable product approach

This is an Author's Accepted Manuscript of an article published in Carlos Perez-Vidal, Luis Gracia, Samuel Sanchez-Caballero, J. Ernesto Solanes, Alessandro Saccon & Josep Tornero (2019) Design of a polishing tool for collaborative robotics using minimum viable product approach, International Journal of Computer Integrated Manufacturing, 32:9, 848-857, DOI: 10.1080/0951192X.2019.1637026 [copyright Taylor & Francis], available online at: http://www.tandfonline.com/10.1080/0951192X.2019.1637026[EN] A collaborative tool for robotic polishing is developed in this work in order to allow the simultaneous operation of the robot system and human operator to cooperatively carry out the polishing task. For this purpose, the collaborative environment is detailed and the polishing application is designed. Moreover, the polishing tool is developed and its implementation using the minimum viable product approach is obtained. Furthermore, a robust hybrid position-force control is proposed to use the developed tool attached to a robot system and some experiments are given to show its performance.This work was supported in part by the Ministerio de Ciencia e Innovacion (Spanish Government) under project [DPI2017-87656-C2-1-R] and the Generalitat Valenciana under Grant [VALi+ d APOSTD/2016/044].Perez-Vidal, C.; Gracia Calandin, LI.; Sanchez-Caballero, S.; Solanes Galbis, JE.; Saccon, A.; Tornero Montserrat, J. (2019). Design of a polishing tool for collaborative robotics using minimum viable product approach. International Journal of Computer Integrated Manufacturing. 32(9):848-857. https://doi.org/10.1080/0951192X.2019.1637026S848857329Alders, K., M. Lehe, and G. Wan. 2001. “Method for the Automatic Recognition of Surface Defects in Body Shells and Device for Carrying Out Said Method” US Patent 6,320,654, Accessed 2001 November. https://www.google.ch/patents/US6320654Alexopoulos, K., Mavrikios, D., & Chryssolouris, G. (2013). ErgoToolkit: an ergonomic analysis tool in a virtual manufacturing environment. International Journal of Computer Integrated Manufacturing, 26(5), 440-452. doi:10.1080/0951192x.2012.731610Andres, J., Gracia, L., & Tornero, J. (2011). Calibration and control of a redundant robotic workcell for milling tasks. International Journal of Computer Integrated Manufacturing, 24(6), 561-573. doi:10.1080/0951192x.2011.566284Arnal, L., Solanes, J. E., Molina, J., & Tornero, J. (2017). Detecting dings and dents on specular car body surfaces based on optical flow. Journal of Manufacturing Systems, 45, 306-321. doi:10.1016/j.jmsy.2017.07.006Blank, S. 2010. “Perfection By Subtraction - The Minimum Feature Set”. Accessed 2018 August. http://steveblank.com/2010/03/04/perfection-by-subtraction-the-minimum-feature-set/Dimeas, F., & Aspragathos, N. (2016). Online Stability in Human-Robot Cooperation with Admittance Control. IEEE Transactions on Haptics, 9(2), 267-278. doi:10.1109/toh.2016.2518670Fitzgerald, C. “Developing Baxter, A new industrial robot with common sense for U.S. manufacturing.” 2013.Gracia, L., Sala, A., & Garelli, F. (2012). A supervisory loop approach to fulfill workspace constraints in redundant robots. Robotics and Autonomous Systems, 60(1), 1-15. doi:10.1016/j.robot.2011.07.008Gracia, L., Sala, A., & Garelli, F. (2014). Robot coordination using task-priority and sliding-mode techniques. Robotics and Computer-Integrated Manufacturing, 30(1), 74-89. doi:10.1016/j.rcim.2013.08.003Gracia, L., Solanes, J. E., Muñoz-Benavent, P., Valls Miro, J., Perez-Vidal, C., & Tornero, J. (2018). Adaptive Sliding Mode Control for Robotic Surface Treatment Using Force Feedback. Mechatronics, 52, 102-118. doi:10.1016/j.mechatronics.2018.04.008Julius, R., Schürenberg, M., Schumacher, F., & Fay, A. (2017). Transformation of GRAFCET to PLC code including hierarchical structures. Control Engineering Practice, 64, 173-194. doi:10.1016/j.conengprac.2017.03.012. E. K. (2016). TOWARDS AN AUTOMATED POLISHING SYSTEM - CAPTURING MANUAL POLISHING OPERATIONS. International Journal of Research in Engineering and Technology, 05(07), 182-192. doi:10.15623/ijret.2016.0507030Khan, A. M., Yun, D., Zuhaib, K. M., Iqbal, J., Yan, R.-J., Khan, F., & Han, C. (2017). Estimation of Desired Motion Intention and compliance control for upper limb assist exoskeleton. International Journal of Control, Automation and Systems, 15(2), 802-814. doi:10.1007/s12555-015-0151-7Kirschner, D., Velik, R., Yahyanejad, S., Brandstötter, M., & Hofbaur, M. (2016). YuMi, Come and Play with Me! A Collaborative Robot for Piecing Together a Tangram Puzzle. Interactive Collaborative Robotics, 243-251. doi:10.1007/978-3-319-43955-6_29Mohammad, A. E. K., Hong, J., & Wang, D. (2018). Design of a force-controlled end-effector with low-inertia effect for robotic polishing using macro-mini robot approach. Robotics and Computer-Integrated Manufacturing, 49, 54-65. doi:10.1016/j.rcim.2017.05.011Nagata, F., Hase, T., Haga, Z., Omoto, M., & Watanabe, K. (2007). CAD/CAM-based position/force controller for a mold polishing robot. Mechatronics, 17(4-5), 207-216. doi:10.1016/j.mechatronics.2007.01.003Nakamura, Y., Hanafusa, H., & Yoshikawa, T. (1987). Task-Priority Based Redundancy Control of Robot Manipulators. The International Journal of Robotics Research, 6(2), 3-15. doi:10.1177/027836498700600201Ries, E. 2009. “What is the Minimum Viable Product”. March. Accessed 2018 August. http://venturehacks.com/articles/minimum-viable-productRobinson, F. 2001 “A Proven Methodology to Maximize Return on Risk”. Accessed 2018 August. http://www.syncdev.com/minimum-viable-productShepherd, S., & Buchstab, A. (2014). KUKA Robots On-Site. Robotic Fabrication in Architecture, Art and Design 2014, 373-380. doi:10.1007/978-3-319-04663-1_26SYMPLEXITY. “Symbiotic Human-Robot Solutions for Complex Surface Finishing Operations.” European project funded by E.U. through the H2020. Project no. 637080. Call: H2020-FoF-2014. Topic: FoF-06-2014. Starting date: 01/ 01/2015.Duration: 48 months. Accessed 2019 March. https://www.symplexity.eu/Vihlborg, P., I. Bryngelsson, B. Lindgren, L. G. Gunnarsson, and P. Graff. 2017. “Associatio between vibration exposure and hand-arm vibration symptoms in a Swedish mechanical industry.” February 2017.Vogel, J., Haddadin, S., Jarosiewicz, B., Simeral, J. D., Bacher, D., Hochberg, L. R., … van der Smagt, P. (2015). An assistive decision-and-control architecture for force-sensitive hand–arm systems driven by human–machine interfaces. The International Journal of Robotics Research, 34(6), 763-780. doi:10.1177/027836491456153

S7A:7 Administration of serpinb3 delays glomerulonephritis and attenuates the lupus-like disease in lupus murine models by an immunomodulatory effect

Background Abnormal apoptosis and clearance of cellular debris concur to development of systemic lupus erythematosus (SLE). SERPINS (serin-protease inhibitors) are ancient molecules regulating immune homeostasis. SERPINB3 modulates apoptosis and is hypoexpressed on SLE B cells. Aim To explore the effects of SERPINB3 administration in murine lupus models, focusing on glomerulonephritis. Methods NZB/W F1 and MRL/lpr mice were used. 40 NZB/W F1 mice were divided into 4 groups of 10 mice each and intraperitoneally injected twice a week starting before occurrence of proteinuria traces (group 1 and 2, prophylactic approach) or after development of proteinuria 30 mg/dl (group 3 and 4, therapeutic approach) with hrSERPINB3 (7.5 µg/0.1 mL prophylactic approach, or 15 µg/0.1 mL therapeutic approach) or PBS (0.1 mL). 20 MRL/lpr mice were injected with hrSERPINB3 (group 5, n=10) or PBS (group 6, n=10) with a prophylactic approach. We assessed time of occurrence and titers of anti-dsDNA and anti-C1q antibodies by ELISA; proteinuria and serum creatinine; overall- and proteinuria-free survival. Six NZB/W F1 mice were sacrificed at week 27, while 10 MRL/lpr mice at week 13 and another 10 at 16/18 weeks for histological kidneys comparison. Flow-cytometry was performed on MRL/lpr splenocytes. Non parametric tests were performed for statistics; proteinuria-free ( Results Levels of autoantibodies were significantly decreased and delayed in group 1 vs group 2, group 3 vs group 4, and group 5 vs group 6 (p Conclusions Administration of SERPINB3 significantly improves disease and delays the onset of severe glomerulonephritis in lupus-prone mice. SERPINB3 may influence immune-cell function through immunoregulatory effects involving promotion of Treg

Early and Late Response and Glucocorticoid-Sparing Effect of Belimumab in Patients with Systemic Lupus Erythematosus with Joint and Skin Manifestations: Results from the Belimumab in Real Life Setting Study—Joint and Skin (BeRLiSS-JS)

Aim. To assess the efficacy of belimumab in joint and skin manifestations in a nationwide cohort of patients with SLE. Methods. All patients with skin and joint involvement enrolled in the BeRLiSS cohort were considered. Belimumab (intravenous, 10 mg/kg) effectiveness in joint and skin manifestations was assessed by DAS28 and CLASI, respectively. Attainment and predictors of DAS28 remission (<2.6) and LDA (≥2.6, ≤3.2), CLASI = 0, 1, and improvement in DAS28 and CLASI indices ≥20%, ≥50%, and ≥70% were evaluated at 6, 12, 24, and 36 months. Results. DAS28 < 2.6 was achieved by 46%, 57%, and 71% of patients at 6, 12, and 24 months, respectively. CLASI = 0 was achieved by 36%, 48%, and 62% of patients at 6, 12, and 24 months, respectively. Belimumab showed a glucocorticoid-sparing effect, being glucocorticoid-free at 8.5%, 15.4%, 25.6%, and 31.6% of patients at 6, 12, 24, and 36 months, respectively. Patients achieving DAS-LDA and CLASI-50 at 6 months had a higher probability of remission at 12 months compared with those who did not (p = 0.034 and p = 0.028, respectively). Conclusions. Belimumab led to clinical improvement in a significant proportion of patients with joint or skin involvement in a real-life setting and was associated with a glucocorticoid-sparing effect. A significant proportion of patients with a partial response at 6 months achieved remission later on during follow-up

A kaleidoscope of photosynthetic antenna proteins and their emerging roles

The latest fundamental knowledge obtained on the light-harvesting mechanisms of the antenna proteins can be bridged to biotechnical optimization of photosynthesis.Photosynthetic light-harvesting antennae are pigment-binding proteins that perform one of the most fundamental tasks on Earth, capturing light and transferring energy that enables life in our biosphere. Adaptation to different light environments led to the evolution of an astonishing diversity of light-harvesting systems. At the same time, several strategies have been developed to optimize the light energy input into photosynthetic membranes in response to fluctuating conditions. The basic feature of these prompt responses is the dynamic nature of antenna complexes, whose function readily adapts to the light available. High-resolution microscopy and spectroscopic studies on membrane dynamics demonstrate the crosstalk between antennae and other thylakoid membrane components. With the increased understanding of light-harvesting mechanisms and their regulation, efforts are focusing on the development of sustainable processes for effective conversion of sunlight into functional bio-products. The major challenge in this approach lies in the application of fundamental discoveries in light-harvesting systems for the improvement of plant or algal photosynthesis. Here, we underline some of the latest fundamental discoveries on the molecular mechanisms and regulation of light harvesting that can potentially be exploited for the optimization of photosynthesis

INTERNATIONAL FINANCIAL REPORTING CONVERGENCE: EVIDENCE FROM THREE CONTINENTAL EUROPEAN COUNTRIES

Listed companies in the European Union are required to apply International Financial Reporting Standards (IFRS) in their consolidated accounts as of 1 January 2005. The purpose of this paper is to compare developments in France, Germany and Italy and the approaches to integrate the current European accounting reform processes with IFRS. The paper evaluates the adaptation of national accounting systems with respect to institutional and regulatory changes on the one hand and financial reporting changes on the other