Identification of red high proper-motion objects in Tycho-2 and 2MASS catalogues using Virtual Observatory tools

Aims: With available Virtual Observatory tools, we looked for new M dwarfs in the solar neighbourhood and M giants with high tangential velocities. Methods: From an all-sky cross-match between the optical Tycho-2 and the near-infrared 2MASS catalogues, we selected objects with proper motions >50mas/yr and very red V-Ks colours. For the most interesting targets, we collected multi-wavelength photometry, constructed spectral energy distributions, estimated effective temperatures and surface gravities from fits to atmospheric models, performed time-series analysis of ASAS V-band light curves, and assigned spectral types from low-resolution spectroscopy obtained with CAFOS at the 2.2m Calar Alto telescope. Results: We got a sample of 59 bright red high proper-motion objects, including fifty red giants, four red dwarfs, and five objects reported in this work for the first time. The five new stars have magnitudes V~10.8-11.3mag, reduced proper motions midway between known dwarfs and giants, near-infrared colours typical of giants, and effective temperatures Teff~2900-3400K. From our time-series analysis, we discovered a long secondary period in Ruber 4 and an extremely long primary period in Ruber 6. With the CAFOS spectra, we confirmed the red giant nature of Ruber 7 and 8, the last of which seems to be one of the brightest metal-poor M giants ever identified.Comment: Accepted in Astronomy & Astrophysic

The triple system HIP96515: a low-mass eclipsing binary with a DB white dwarf companion

HIP96515A is a double-lined spectroscopic binary with a visual companion (HIP96515B) at 8.6 arcsec. It is included in the SACY catalog as a potential young star and classified as an eclipsing binary in the ASAS Catalog. We have analyzed spectroscopic and photometric observations of the triple system. The high-resolution optical spectrum of HIP96515A has been used to derive a mass ratio, M_2/M_1, close to 0.9, with the SB2 components showing spectral types of M1 and M2. The ASAS and Hipparcos light-curves of HIP96515A show periodic variations with P=2.3456 days, confirming that HIP96515A is an eclipsing binary with preliminary parameters of i=89, M_Aa=0.59+-0.03 Msun and M_Ab=0.54+-0.03 Msun, for the primary and secondary, respectively, at an estimated distance of 42+-3 pc. This is a new eclipsing binary with component masses below 0.6 Msun. Multi-epoch observations of HIP 96515 A&B show that the system is a common proper motion pair. The optical spectrum of HIP 96515B is consistent with a pure helium atmosphere (DB) white dwarf. We estimate a total age (main-sequence lifetime plus cooling age) of 400 Myr for the white dwarf. If HIP 96515 A&B are coeval, and assuming a common age of 400 Myr, the comparison of the masses of the eclipsing binary members with evolutionary tracks shows that they are underestimated by ~15% and ~10%, for the primary and secondary, respectively.Comment: Accepted for publication in A&A, 9 pages, 9 figure

BACE1 RNAi restores the composition of phosphatidylethanolamine-derivates related to memory improvement in aged 3xTg-AD mice

ABSTRACT: β-amyloid (Aβ) is produced by the β-secretase 1 (BACE1)-mediated enzymatic cleavage of the amyloid precursor protein through the amyloidogenic pathway, making BACE1 a therapeutic target against Alzheimer’s disease (AD). Alterations in lipid metabolism are a risk factor for AD by an unknown mechanism. The objective of this study was to determine the effect of RNA interference against BACE1 (shBACEmiR) on the phospholipid profile in hippocampal CA1 area in aged 3xTg-AD mice after 6 and 12 months of treatment compared to aged PS1KI mice. The shBACEmiR treatment induced cognitive function recovery and restored mainly the fatty acid composition of lysophosphatidylethanolamine and etherphosphatidylethanolamine, reduced the cPLA2’s phosphorylation, down-regulated the levels of arachidonic acid and COX2 in the hippocampi of 3xTg-AD mice. Together, our findings suggest, for the first time, that BACE1 silencing restores phospholipids composition which could favor the recovery of cellular homeostasis and cognitive function in the hippocampus of triple transgenic AD mice. Keywords: Alzheimer’s disease, phospholipids, BACE1, RNA interference, hippocampus, cognitive functio

Guidelines and Recommendations on Yeast Cell Death Nomenclature

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cellular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the definition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death routines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the authors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the progress of this vibrant field of research

Diacylglycerol triggers Rim101 pathway dependent necrosis in yeast: a model for lipotoxicity

The loss of lipid homeostasis can lead to lipid overload and is associated with a variety of disease states. However, little is known as to how the disruption of lipid regulation or lipid overload affects cell survival. In this study we investigated how excess diacylglycerol (DG), a cardinal metabolite suspected to mediate lipotoxicity, compromises the survival of yeast cells. We reveal that increased DG achieved by either genetic manipulation or pharmacological administration of 1,2-dioctanoyl-sn-glycerol (DOG) triggers necrotic cell death. The toxic effects of DG are linked to glucose metabolism and require a functional Rim101 signaling cascade involving the Rim21 dependent sensing complex and activation of a calpain-like protease. The Rim101 cascade is an established pathway that triggers a transcriptional response to alkaline or lipid stress. We propose that the Rim101 pathway senses DG-induced lipid perturbation and conducts a signaling response that either facilitates cellular adaptation or triggers lipotoxic cell death. Using established models of lipotoxicity i.e. high fat diet in Drosophila and palmitic acid administration in cultured human endothelial cells, we present evidence that the core mechanism underlying this calpain-dependent lipotoxic cell death pathway is phylogenetically conserved

Guidelines and recommendations on yeast cell death nomenclature

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cel-lular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the defi-nition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differ-ential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death rou-tines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the au-thors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the pro-gress of this vibrant field of research

A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK-signalling and aberrant lipid homeostasis.

These original data support the publication "A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK-signalling and aberrant lipid homeostasis." by Davis et al. published in iScience.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Quantifying yeast lipidomics by high performance thin layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics.

Here we present evidence that high performance thin layer chromatography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV