ISOLAMENTO DO Ureaplasma diversum EM MUCO VULVOVAGINAL DE VACAS LEITEIRAS REPETIDORAS DE ESTRO NO ESTADO DE ALAGOAS BRASIL

Ureaplasma diversum is an opportunistic bacteria of the bovine genital tract and an important agent in granular vulvovaginitis, abortion, low fertility herd rate and respiratory problems in calves. The aim of this work was to study the relationship between positive cultures for Ureaplasma diversum and the extension and severity of the lesions in the vaginal mucosa of repeat breeders dairy cows in Alagoas State, Brazil. Visual evaluation were performed in 93 cows to evaluate the vaginal lesion score (VLS), classified according the lesion severity and extension (0 to 4, with 0= no lesions, 4= severe inflammation and local necrosis). The VLS 1 was the most frequent (51,61%). VLS 0 was present in 17,2% of the females, while 29,03% and 2,16% showed VLS 2 and 3, respectively. None of the cows showed VLS 4. Vaginal swab samples were obtained from 24 females (25%). Of them, 41,7% were positive for U. diversum. The presence of U. diversum was associated positively to VLS, especially to those cows showing vaginal lesion score 1. The present results indicated that Ureaplasma diversum should be considered responsible for low reproductive efficiency, carrying out to repeat breeding, probably because of embryo mortality.O Ureaplasma diversum é um patógeno oportunista do trato genital dos bovinos que causa surtos de vulvovaginite granular, seguidos de abortamento e redução da eficiência reprodutiva do rebanho. O presente estudo foi desenvolvido com o objetivo de avaliar a relação entre a extensão e a gravidade das lesões de vulvovaginite observadas com o isolamento de Ureaplasma diversum em amostras colhidas diretamente da mucosa vulvovaginal, em fêmeas de aptidão leiteira repetidoras de estro no estado de Alagoas, Brasil. A avaliação da extensão e severidade das lesões foi feita em 93 vacas com histórico de repetição regular de estro após três ou mais inseminações artificiais, seguindo-se a seguinte metodologia de classificação: graus de 0 a 4, sendo 0=sem lesões; 4=lesões severas, com hemorragia e necrose. Dos 93 animais avaliados, 17,2% apresentavam lesão grau 0; 51,61% grau 1; 29,03% grau 2; 2,16% grau 3 e nenhum animal mostrou lesão de grau 4. Foi colhido muco vulvovaginal de 24 vacas, escolhidas aleatoriamente, para processamento bacteriológico, encontrando-se 41,67% das amostras positivas para Ureaplasma diversum. O isolamento do agente esteve positivamente relacionado à presença de lesões na mucosa vulvovaginal, especialmente àquelas de grau 1. Os resultados deste estudo indicam que o Ureaplasma diversum deve ser considerado como agente responsável por queda na eficiência reprodutiva, promovendo retorno ao estro, provavelmente em conseqüência de mortalidade embrionária

INFUSÃO UTERINA COM BIGUANIDA POLIMÉRICA SOBRE A COLONIZAÇÃO BACTERIANA DO ÚTERO DE VACAS COM INFECÇÃO PUERPERAL APÓS RETENÇÃO DE ENVOLTÓRIOS FETAIS

This study was carried out with the aim to verify the utilization viability of polymeric biguanide solutions for the topic treatment of puerperal uterine infections in bovine females after premature parturition. Twenty Girolando cows of a dairy farm, with placenta retention and metritis were randomly distributed in two groups. Group 1, constituted of cows that received three uterine infusions of a polymeric biguanide solution, and Group 2, with animals that were not submitted to any treatment. There were taken uterine content samples at the first, third and fifth post partum weeks for bacteriological cultures, that means, before the first infusion, 14 and 28 days after treatment. The founds of bacteriological cultures in the two groups demonstrated that the solution of polymeric biguanide was not effective to reduce uterine bacteriological colonization in cows with post partum infections.Com o objetivo de estudar a viabilidade da utilização de biguanida polimérica, no tratamento tópico de infecções puerperais em fêmeas bovinas após o parto prematuro, 20 fêmeas Girolando pertencentes a um sistema de produção leiteira, que apresentaram retenção de envoltórios fetais e conseqüentemente endometrite puerperal, foram distribuídas aleatoriamente em dois grupos. O Grupo I, foi constituído de animais que receberam três infusões uterinas de biguanida polimérica; e o Grupo 2, animais não tratados. Foram realizadas colheitas de conteúdo uterino na primeira, terceira e quinta semanas pós-parto para realização de cultivos bacteriológicos, ou seja, antes da infusão, aos 14 e 28 dias após tratamento medicamentoso. O resultado dos cultivos bacteriológicos nos dois grupos demonstrou que a utilização da biguanida polimérica não reduziu a colonização bacteriana no útero dos animais tratados, em comparação àqueles não tratados

Lentinus crinitus basidiocarp stipe and pileus: chemical composition, cytotoxicity and antioxidant activity

Lentinus crinitus is a wild fungus, which produces mushrooms consumed by some Amazonian Indians. Besides, it is recognized for its diverse biological activities and biotechnological applications. However, there are few reports with limited information on basidiocarp chemical composition and cytotoxicity. Our study determined and evaluated the chemical composition, cytotoxicity, and antioxidant activity of L. crinitus pileus and stipe separately. Chromatographic methods were used to evaluate basidiocarp chemical composition. Cytotoxicity was verified using a cell culture from porcine liver and against a panel of human tumor cells from different models. Antioxidant activity was assessed by different in vitro methods. The pileus had higher levels of protein, ash, tocopherols, and organic acids, mainly malic acid, than the stipe. The stipe revealed higher contents of carbohydrates, energy, soluble sugars, and phenolic acids, mostly p-hydroxybenzoic acid. L. crinitus basidiocarp has mainly trehalose as soluble sugar, and less than 1% fat being ~60% polyunsaturated fatty acids (mostly linoleic and oleic acids), and ~13% saturated fatty acids (mostly palmitic acid). L. crinitus revealed high antioxidant activity for most methods and no cytotoxic activity against tumor and non-tumor cells. L. crinitus basidiocarp can be considered a functional food with applicability in food, cosmetic, and pharmaceutical industries. Graphic abstract: [Figure not available: see fulltext.]This research was supported by Universidade Paranaense, UniCesumar, Polytechnic Institute of Bragança, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—finance code 001—, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Araucária. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); to the national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros contract.info:eu-repo/semantics/publishedVersio

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02