127 research outputs found
No Differential Regulation of Dopamine Transporter (DAT) and Vesicular Monoamine Transporter 2 (VMAT2) Binding in a Primate Model of Parkinson Disease
Radioligands for DAT and VMAT2 are widely used presynaptic markers for assessing dopamine (DA) nerve terminals in Parkinson disease (PD). Previous in vivo imaging and postmortem studies suggest that these transporter sites may be regulated as the numbers of nigrostriatal neurons change in pathologic conditions. To investigate this issue, we used in vitro quantitative autoradioradiography to measure striatal DAT and VMAT2 specific binding in postmortem brain from 14 monkeys after unilateral internal carotid artery infusion of 1-Methyl-4-Phenyl-1,2,3,6-tetrahydropyridine (MPTP) with doses varying from 0 to 0.31 mg/kg. Quantitative estimates of the number of tyrosine hydroxylase (TH)-immunoreactive (ir) neurons in substantia nigra (SN) were determined with unbiased stereology, and quantitative autoradiography was used to measure DAT and VMAT2 striatal specific binding. Striatal VMAT2 and DAT binding correlated with striatal DA (rs = 0.83, rs = 0.80, respectively, both with n = 14, p<0.001) but only with nigra TH-ir cells when nigral cell loss was 50% or less (r = 0.93, n = 8, p = 0.001 and r = 0.91, n = 8, p = 0.002 respectively). Reduction of VMAT2 and DAT striatal specific binding sites strongly correlated with each other (r = 0.93, n = 14, p<0.0005). These similar changes in DAT and VMAT2 binding sites in the striatal terminal fields of the surviving nigrostriatal neurons demonstrate that there is no differential regulation of these two sites at 2 months after MPTP infusion
Preoperative Chemoradiotherapy Does Not Necessarily Reduce Lymph Node Retrieval in Rectal Cancer Specimens—Results from a Prospective Evaluation with Extensive Pathological Work-up
Improving biomass production and saccharification in Brachypodium distachyon through overexpression of a sucrose-phosphate synthase from sugarcane
The substitution of fossil by renewable energy sources is a major strategy in reducing CO2 emission and mitigating climate change. In the transport sector, which is still mainly dependent on liquid fuels, the production of second generation ethanol from lignocellulosic feedstock is a promising strategy to substitute fossil fuels. The main prerequisites on designated crops for increased biomass production are high biomass yield and optimized saccharification for subsequent use in fermentation processes. We tried to address these traits by the overexpression of a sucrose-phosphate synthase gene (SoSPS) from sugarcane (Saccharum officinarum) in the model grass Brachypodium distachyon. The resulting transgenic B. distachyon lines not only revealed increased plant height at early growth stages but also higher biomass yield from fully senesced plants, which was increased up to 52 % compared to wild-type. Additionally, we determined higher sucrose content in senesced leaf biomass from the transgenic lines, which correlated with improved biomass saccharification after conventional thermo-chemical pretreatment and enzymatic hydrolysis. Combining increased biomass production and saccharification efficiency in the generated B. distachyon SoSPS overexpression lines, we obtained a maximum of 74 % increase in glucose release per plant compared to wild-type. Therefore, we consider SoSPS overexpression as a promising approach in molecular breeding of energy crops for optimizing yields of biomass and its utilization in second generation biofuel production
Chromatin-associated regulation of sorbitol synthesis in flower buds of peach
[EN] Key message PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications.
Abstract Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to survive low temperatures and water deprivation during winter. A seasonal period of dormancy further improves tolerance of buds to environmental stresses through specific mechanisms poorly known at the molecular level. We have shown that peach PpeS6PDH gene is down-regulated in flower buds after dormancy release, concomitantly with changes in the methylation level at specific lysine residues of histone H3 (H3K27 and H3K4) in the chromatin around the translation start site of the gene. PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate dehydrogenase, the key enzyme for biosynthesis of sorbitol. Consistently, sorbitol accumulates in dormant buds showing higher PpeS6PDH expression. Moreover, PpeS6PDH gene expression is affected by cold and water deficit stress. Particularly, its expression is up-regulated by low temperature in buds and leaves, whereas desiccation treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of chromatin modification mechanisms, transcriptional regulation of PpeS6PDH and sorbitol accumulation in flower buds of peach. In addition to its role as a major translocatable photosynthate in Rosaceae species, sorbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tolerance to environmental stresses in flower buds of peach.This work was funded by the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)-FEDER (RF2013-00043-C02-02) and the Ministry of Science and Innovation of Spain (AGL2010-20595). AL was funded by a fellowship co-financed by the European Social Fund and the Instituto Valenciano de Investigaciones Agrarias (IVIA).Lloret, A.; Martinez Fuentes, A.; Agustí Fonfría, M.; Badenes, ML.; Rios, G. (2017). Chromatin-associated regulation of sorbitol synthesis in flower buds of peach. Plant Molecular Biology. 95(4-5):507-517. https://doi.org/10.1007/s11103-017-0669-6S507517954-5Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250. doi: 10.1158/0008-5472.CAN-04-0496Bai S, Saito T, Ito A et al (2016) Small RNA and PARE sequencing in flower bud reveal the involvement of sRNAs in endodormancy release of Japanese pear (Pyrus pyrifolia ‘Kosui’). BMC Genomics 17:230. doi: 10.1186/s12864-016-2514-8Bielenberg DG, Wang Y, Li Z et al (2008) Sequencing and annotation of the evergrowing locus in peach (Prunus persica [L.] Batsch) reveals a cluster of six MADS-box transcription factors as candidate genes for regulation of terminal bud formation. Tree Genet Genomes 4:495–507. doi: 10.1007/s11295-007-0126-9Bieleski RL (1969) Accumulation and translocation of sorbitol in apple phloem. Aust J Biol Sci 22:611–620. doi: 10.1071/BI9690611Bieleski RL (1982) Sugar alcohols. In: Loewus F, Tanner W (eds) Encyclopedia of plant physiology, new series 13A. Springer-Verlag, Berlin, pp 158–192Bortiri E, Oh SH, Gao FY, Potter D (2002) The phylogenetic utility of nucleotide sequences of sorbitol 6-phosphate dehydrogenase in Prunus (Rosaceae). Am J Bot 89:1697–1708. doi: 10.3732/ajb.89.10.1697Chouard P (1960) Vernalization and its relations to dormancy. Annu Rev Plant Physiol 11:191–238. doi: 10.1146/annurev.pp.11.060160.001203Conde D, Le Gac AL, Perales M et al (2017) Chilling-responsive DEMETER-LIKE DNA demethylase mediates in poplar bud break. Plant Cell Environ 40:2236–2249. doi: 10.1111/pce.13019Couvillon GA, Erez A (1985) Influence of prolonged exposure to chilling temperatures on bud break and heat requirement for bloom of several fruit species. J Amer Soc Hort Sci 110:47–50de la Fuente L, Conesa A, Lloret A, Badenes ML, Ríos G (2015) Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach. Tree Genet Genomes 11:45. doi: 10.1007/s11295-015-0869-7Deng W, Buzas DM, Ying H et al (2013) Arabidopsis polycomb repressive complex 2 binding sites contain putative GAGA factor binding motifs within coding regions of genes. BMC Genomics 14:593. doi: 10.1186/1471-2164-14-593Escobar-Gutiérrez AJ, Gaudillère JP (1996) Distribution, metabolism and role of sorbitol in higher plants—A review. Agronomie 16:281–298. doi: 10.1051/agro:19960502Escobar-Gutiérrez AJ, Zipperlin B, Carbonne F, Moing A, Gaudillére JP (1998) Photosynthesis, carbon partitioning and metabolite content during drought stress in peach seedlings. Aust J Plant Physiol 25:197–205. doi: 10.1071/PP97121Eshghi S, Tafazoli E, Dokhani S, Rahemi M, Emam Y (2007) Changes in carbohydrate contents in shoot tips, leaves and roots of strawberry (Fragaria x ananassa Duch) during flower-bud differentiation. Sci Hortic 113:255–260. doi: 10.1016/j.scienta.2007.03.014Everard JD, Cantini C, Grumet R, Plummer J, Loescher WH (1997) Molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery. Plant Physiol 113:1427–1435. doi: 10.1104/pp.113.4.1427Fennell A (2014) Genomics and functional genomics of winter low temperature tolerance in temperate fruit crops. Crit Rev Plant Sci 33:125–140. doi: 10.1080/07352689.2014.870410Figueroa CM, Iglesias AA (2010) Aldose-6-phosphate reductase from apple leaves: importance of the quaternary structure for enzyme activity. Biochimie 92:81–88. doi: 10.1016/j.biochi.2009.09.013Gao M, Tao R, Miura K, Dandekar AM, Sugiura A (2001) Transformation of Japanese persimmon (Diospyros kaki Thunb) with apple cDNA encoding NADP-dependent sorbitol-6-phosphate dehydrogenase. Plant Sci 160:837–845. doi: 10.1016/S0168-9452(00)00458-1Grant CR, ap Rees T (1981) Sorbitol metabolism by apple seedlings. Phytochemistry 20:1505–1511. doi: 10.1016/S0031-9422(00)98521-2Hartman MD, Figueroa CM, Arias DG, Iglesias AA (2017) Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants. Plant Cell Physiol 58:145–155. doi: 10.1093/pcp/pcw180Horvath DP, Anderson JV, Chao WS, Foley ME (2003) Knowing when to grow: signals regulating bud dormancy. Trends Plant Sci 8:534–540. doi: 10.1016/j.tplants.2003.09.013Horvath DP, Sung S, Kim D, Chao W, Anderson J (2010) Characterization, expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge. Plant Mol Biol 73:169–179. doi: 10.1007/s11103-009-9596-5Hussain S, Niu Q, Yang F, Hussain N, Teng Y (2015) The possible role of chilling in floral and vegetative bud dormancy release in Pyrus pyrifolia. Biol Plant 59:726–734. doi: 10.1007/s10535-015-0547-5Hyndman D, Baumanb DR, Herediac VV, Penning TM (2003) The aldo-keto reductase superfamily homepage. Chem Biol Interact 143–144:621–631. doi: 10.1016/S0009-2797(02)00193-XIto A, Sakamoto D, Moriguchi T (2012) Carbohydrate metabolism and its possible roles in endodormancy transition in Japanese pear. Sci Hortic 144:187–194. doi: 10.1016/j.scienta.2012.07.009Ito A, Sugiura T, Sakamoto D, Moriguchi T (2013) Effects of dormancy progression and low-temperature response on changes in the sorbitol concentration in xylem sap of Japanese pear during winter season. Tree Physiol 33:398–408. doi: 10.1093/treephys/tpt021Jung S, Bassett C, Bielenberg DG et al (2015) A standard nomenclature for gene designation in the Rosaceae. Tree Genet Genomes 11:108. doi: 10.1007/s11295-015-0931-5Kanayama Y, Mori H, Imaseki H, Yamaki S (1992) Nucleotide sequence of a cDNA encoding NADP-sorbitol-6-phosphate dehydrogenase from apple. Plant Physiol 100:1607–1608Kanayama Y, Watanabe M, Moriguchi R, Deguchi M, Kanahama K, Yamaki S (2006) Effects of low temperature and abscisic acid on the expression of the sorbitol-6-phosphate dehydrogenase gene in apple leaves. J Japan Soc Hort Sci 75:20–25. doi: 10.2503/jjshs.75.20Kumar G, Rattan UK, Singh AK (2016a) Chilling-mediated DNA methylation changes during dormancy and its release reveal the importance of epigenetic regulation during winter dormancy in apple (Malus x domestica Borkh). PLoS ONE 11:e0149934. doi: 10.1371/journal.pone.0149934Kumar S, Stecher G, Tamura K (2016b) MEGA7: molecular evolutionary genetics analysis version 70 for bigger datasets. Mol Biol Evol 33:1870–1874. doi: 10.1093/molbev/msw054Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685. doi: 10.1038/227680a0Larkin MA, Blackshields G, Brown NP et al (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948. doi: 10.1093/bioinformatics/btm404Leida C, Terol J, Martí G et al (2010) Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization. Tree Physiol 30:655–666. doi: 10.1093/treephys/tpq008Leida C, Conesa A, Llácer G, Badenes ML, Ríos G (2012) Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner. New Phytol 193:67–80. doi: 10.1111/j.1469-8137.2011.03863.xLiang D, Cui M, Wu S, Ma F-W (2012) Genomic structure, sub-cellular localization, and promoter analysis of the gene encoding sorbitol-6-phosphate dehydrogenase from apple. Plant Mol Biol Rep 30:904–914. doi: 10.1007/s11105-011-0409-zLiu D, Ni J, Wu R, Teng Y (2013) High temperature alters sorbitol metabolism in Pyrus pyrifolia leaves and fruit flesh during late stages of fruit enlargement. J Am Soc Hortic Sci 138:443–451Lloret A, Conejero A, Leida C et al (2017) Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus. Sci Rep 7:332. doi: 10.1038/s41598-017-00471-7Lo Bianco R, Rieger M, Sung S-JS (2000) Effect of drought on sorbitol and sucrose metabolism in sinks and sources of peach. Physiol Plant 108:71–78. doi: 10.1034/j.1399-3054.2000.108001071.xLoescher WH (1987) Physiology and metabolism of sugar alcohols in higher-plants. Physiol Plant 70:553–557. doi: 10.1111/j.1399-3054.1987.tb02857.xLoescher WH, Marlow GC, Kennedy RA (1982) Sorbitol metabolism and sink-source interconversions in developing apple leaves. Plant Physiol 70:335–339. doi: 10.1104/pp.70.2.335Marquat C, Vandamme M, Gendraud M, Pétel G (1999) Dormancy in vegetative buds of peach: relation between carbohydrate absorption potentials and carbohydrate concentration in the bud during dormancy and its release. Sci Hortic 79:151–162. doi: 10.1016/S0304-4238(98)00203-9Niu Q, Li J, Cai D et al (2016) Dormancy-associated MADS-box genes and microRNAs jointly control dormancy transition in pear (Pyrus pyrifolia white pear group) flower bud. J Exp Bot 67:239–257. doi: 10.1093/jxb/erv454Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper—excel-based tool using pair-wise correlations. Biotechnol Lett 26:509–515. doi: 10.1023/B:BILE.0000019559.84305.47Ríos G, Leida C, Conejero A, Badenes ML (2014) Epigenetic regulation of bud dormancy events in perennial plants. Front Plant Sci 5:247. doi: 10.3389/fpls.2014.00247Saito T, Bai S, Imai T, Ito A, Nakajima I, Moriguchi T (2015) Histone modification and signalling cascade of the dormancy-associated MADS-box gene, PpMADS13-1, in Japanese pear (Pyrus pyrifolia) during endodormancy. Plant Cell Environ 38:1157–1166. doi: 10.1111/pce.12469Santamaría ME, Hasbún R, Valera MJ et al (2009) Acetylated H4 histone and genomic DNA methylation patterns during bud set and bud burst in Castanea sativa. J Plant Physiol 166:1360–1369. doi: 10.1016/j.jplph.2009.02.014Shen B, Hohmann S, Jensen RG, Bohnert HJ (1999) Roles of sugar alcohols in osmotic stress adaptation replacement of glycerol by mannitol and sorbitol in yeast. Plant Physiol 121:45–52. doi: 10.1104/pp.121.1.45Sheveleva EV, Marquez S, Chmara W, Zegeer A, Jensen RG, Bohnert HJ (1998) Sorbitol-6-phosphate dehydrogenase expression in transgenic tobacco high amounts of sorbitol lead to necrotic lesions. Plant Physiol 117:831–839. doi: 10.1104/pp.117.3.831Silver N, Best S, Jian J, Thein SL (2006) Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol 7:33. doi: 10.1186/1471-2199-7-33Talavera G, Castresana J (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol 56:564–577. doi: 10.1080/10635150701472164Tao R, Uratsu SL, Dandekar AM (1995) Sorbitol synthesis in transgenic tobacco with apple cDNA encoding NADP-dependent sorbitol-6-phosphate dehydrogenase. Plant Cell Physiol 36:525–532. doi: 10.1093/oxfordjournals.pcp.a078789Teo G, Suzuki Y, Uratsu SL et al (2006) Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality. Proc Natl Acad Sci USA 103:18842–18847. doi: 10.1073/pnas.0605873103Trotel P, Bouchereau A, Niogret MF, Larher F (1996) The fate of osmo-accumulated proline in leaf discs of Rape (Brassica napus L) incubated in a medium of low osmolarity. Plant Sci 118:31–45. doi: 10.1016/0168-9452(96)04422-6Verde I, Abbott AG, Scalabrin S et al (2013) The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat Genet 45:487–494. doi: 10.1038/ng.2586Webb KL, Burley JWA (1962) Sorbitol translocation in apple. Science 137:766. doi: 10.1126/science.137.3532.766Wisniewski M, Norelli J, Artlip T (2015) Overexpression of a peach CBF gene in apple: a model for understanding the integration of growth, dormancy, and cold hardiness in woody plants. Front Plant Sci 6:85. doi: 10.3389/fpls.2015.00085Yadav R, Prasad R (2014) Identification and functional characterization of sorbitol-6-phosphate dehydrogenase protein from rice and structural elucidation by in silico approach. Planta 240:223–238. doi: 10.1007/s00425-014-2076-
A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)
Meeting abstrac
Multi-messenger observations of a binary neutron star merger
On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ~1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40+8-8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 Mo. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ~40 Mpc) less than 11 hours after the merger by the One- Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ~10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position ~9 and ~16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta
Identification and characterization of the microRNA transcriptome of a moth orchid Phalaenopsis aphrodite
Modeling the Impact of Climate and Landscape on the Efficacy of White Tailed Deer Vaccination for Cattle Tick Control in Northeastern Mexico
Comparing injecting and non-injecting illicit opioid users in a multi-site Canadian sample (OPICAN cohort)
Illicit opioid use in Canada and elsewhere increasingly involves a variety of opioids and non-injection routes of administration. Injection and non-injection opioid users tend to differ in various key characteristics. From a public health perspective, non-injection routes of opioid use tend to be less harmful due to lesser morbidity and mortality risks. Our study compared current injectors (80%) and non-injectors (20%) in a multi-site sample of regular illicit opioid users from across Canada ('OPICAN' study). In bivariate analysis, injectors and non-injectors differed by prevalence in social and health characteristics as well as drug use. Logistic regression analysis identified city, drug use, housing status and mental health problems as independent predictors of injection status. Further analysis revealed that the majority of current non-injectors had an injection history. Our results reinforce the need to explore potential interventions aimed at preventing the transition from non-injectors to injecting, or facilitating the transition of injectors to non-injecting, as initiated in several other contexts
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