121 research outputs found
On Adaptive Confidences for Critic-Driven Classifier Combining
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2005
- Field of study
Free-hand sketch recognition by multi-kernel feature learning
- Author
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/08/2015
- Field of study
Abstract Free-hand sketch recognition has become increasingly popular due to the recent expansion of portable touchscreen devices. However, the problem is non-trivial due to the complexity of internal structures that leads to intra-class variations, coupled with the sparsity in visual cues that results in inter-class ambiguities. In order to address the structural complexity, a novel structured representation for sketches is proposed to capture the holistic structure of a sketch. Moreover, to overcome the visual cue sparsity problem and therefore achieve state-of-the-art recognition performance, we propose a Multiple Kernel Learning (MKL) framework for sketch recognition, fusing several features common to sketches. We evaluate the performance of all the proposed techniques on the most diverse sketch dataset to date (Mathias et al., 2012), and offer detailed and systematic analyses of the performance of different features and representations, including a breakdown by sketch-super-category. Finally, we investigate the use of attributes as a high-level feature for sketches and show how this complements low-level features for improving recognition performance under the MKL framework, and consequently explore novel applications such as attribute-based retrieval
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Structural basis of nSH2 regulation and lipid binding in PI3Kα
- Author
- Publication venue
- Impact Journals LLC
- Publication date
- 01/10/2014
- Field of study
We report two crystal structures of the wild-type phosphatidylinositol 3-kinase α (PI3Kα) heterodimer refined to 2.9 Ă
and 3.4 Ă
resolution: the first as the free enzyme, the second in complex with the lipid substrate, diC4-PIP2, respectively. The first structure shows key interactions of the N-terminal SH2 domain (nSH2) and iSH2 with the activation loop that suggest a mechanism by which the enzyme is inhibited in its basal state. In the second structure, the lipid substrate binds in a positively charged pocket adjacent to the ATP-binding site, bordered by the P-loop, the activation loop and the iSH2 domain. An additional lipid-binding site was identified at the interface of the ABD, iSH2 and kinase domains. The ability of PI3Kα to bind an additional PIP2 molecule was confirmed in vitro by fluorescence quenching experiments. The crystal structures reveal key differences in the way the nSH2 domain interacts with wild-type p110α and with the oncogenic mutant p110αH1047R. Increased buried surface area and two unique salt-bridges observed only in the wild-type structure suggest tighter inhibition in the wild-type PI3Kα than in the oncogenic mutant. These differences may be partially responsible for the increased basal lipid kinase activity and increased membrane binding of the oncogenic mutant
Photo-antagonism of the GABAA receptor
- Author
- A Sali
- A Triller
- B Frolund
- C Notredame
- CG Wermuth
- CL Padgett
- CM Houston
- D Belelli
- DA Wagner
- DM Kullmann
- E Sigel
- EF Pettersen
- EG Steward
- EM Schneider Gasser
- EO Mann
- F Iqbal
- G Jones
- G Nyiri
- H Kiefer
- H Mohler
- H Zhong
- J Amin
- J Isaacson
- JG Newell
- JG Newell
- JH Holden
- JJ Chambers
- JM Fritschy
- JQ Kang
- JT Kittler
- K Ramamoorthi
- L Dubinsky
- LB Kier
- M Ernst
- M Heaulme
- M Mortensen
- M Mortensen
- M Mortensen
- M Mortensen
- MA Simmonds
- MC Ashby
- N Zacharias
- NG Bowery
- O Arunlakshana
- P Thomas
- PS Miller
- R Sathyapriya
- RW Olsen
- S Ueno
- SB Hansen
- SC Lummis
- SE Westh-Hansen
- SE Westh-Hansen
- SJ Moss
- T Klausberger
- T Klausberger
- TC Turek
- U Resch-Genger
- Y Bogdanov
- Y Chang
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/07/2014
- Field of study
Neurotransmitter receptor trafficking is fundamentally important for synaptic transmission and neural network activity. GABAA receptors and inhibitory synapses are vital components of brain function, yet much of our knowledge regarding receptor mobility and function at inhibitory synapses is derived indirectly from using recombinant receptors, antibody-tagged native receptors and pharmacological treatments. Here we describe the use of a set of research tools that can irreversibly bind to and affect the function of recombinant and neuronal GABAA receptors following ultraviolet photoactivation. These compounds are based on the competitive antagonist gabazine and incorporate a variety of photoactive groups. By using site-directed mutagenesis and ligand-docking studies, they reveal new areas of the GABA binding site at the interface between receptor ÎČ and α subunits. These compounds enable the selected inactivation of native GABAA receptor populations providing new insight into the function of inhibitory synapses and extrasynaptic receptors in controlling neuronal excitation
Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints
- Author
- A Jurisicova
- A Walker
- AA Jeyaprakash
- AA Kiessling
- AA Kiessling
- AA Kiessling
- AA Kiessling
- AD Carter
- AJ Baumgarten
- AM Kocabas
- Ann A. Kiessling
- AS Hemerly
- AT Dobson
- AW Bird
- AW Murray
- BH Miller
- BL Kidder
- Bryan Desmarais
- C Klebig
- C Marquez
- CA Cowan
- CA Schatz
- Christina Mara
- CL Sansam
- DG Simmons
- Dimitris Loutradis
- DJ Baker
- DJ Pugh
- DR Foltz
- EC Schirmer
- EE McCarthy
- EM Dunleavy
- G Vlotides
- H Izumi
- H Lee
- HC Hsu
- HC Hwang
- J Du
- J Kim
- J Rhodes
- J Sage
- J You
- JA Thomson
- K Bracey
- K Buchet-Poyau
- K Hardy
- K Hardy
- K Takahashi
- K Tsunemoto
- K Yagita
- K Yuan
- KE Nichols
- KM Gilljam
- Kostas Kallianidis
- KU Hong
- L Macurek
- M Bielanska
- M Li
- M Luna
- M Mukherji
- M Paramasivam
- MJ OâConnell
- MP Dekens
- N Iwamori
- N Yoshizawa-Sugata
- O Tsinkalovsky
- PJ Tesar
- Q Zhang
- R Gassmann
- R Hu
- R Kittler
- R Xie
- Ritsa Bletsa
- RL Ferguson
- S Cai
- S Paixao
- S Ryser
- SM Cutts
- SN Naryzhny
- T Kato
- T Matsuo
- W Zhu
- WC Earnshaw
- X Liu
- XC Wang
- Y Geng
- Y Sun
- Z Bar-Joseph
- Z Ullah
- Publication venue
- Springer US
- Publication date
- 01/01/2010
- Field of study
Mechanisms of metastasis
- Author
- A Bendich
- A Bergsmedh
- A Chakraborty
- A Ito
- A Vignery
- AB Al-Mehdi
- AE Lagarde
- AF Chambers
- AF Chambers
- AJ Minn
- AJ Minn
- CT Guy
- CW Wong
- D Garcia-Olmo
- D Tarin
- D Tarin
- DC Garcia-Olmo
- DE Johnson
- DL Trainer
- DM Goldenberg
- DS Nuyten
- EC Kauffman
- EE Schadt
- EJ Chesler
- EY Lin
- FR Miller
- G Riethmuller
- GL Nicolson
- H Stopper
- H Yang
- HY Chang
- IJ Fidler
- IJ Fidler
- IJ Fidler
- IJ Fidler
- J Jami
- J Pawelek
- JB Wyckoff
- JE Talmadge
- JF Harris
- JM Anderson
- JM Pawelek
- JM Pawelek
- Jude Alsarraj
- JW Pollard
- K Okazaki
- K Wolf
- Kent W Hunter
- KJ Luzzi
- L Bystrykh
- L Olsson
- L Weiss
- L Weiss
- LA Liotta
- LJ van't Veer
- LT Busund
- M Ishikawa
- M Mori
- M Munzarova
- M Rachkovsky
- N Sekita
- Nigel PS Crawford
- NP Crawford
- NR Pratt
- O Schmidt-Kittler
- P De Baetselier
- P Frost
- P Loustalot
- PC Nowell
- PS Steeg
- R Bernards
- R Heimann
- R Heimann
- RN Kaplan
- RP Hill
- RS Kerbel
- RS Kerbel
- S Paget
- S Paik
- S Ramaswamy
- SA Kramer
- SA Leon
- ST Cheung
- T Lifsted
- T Nakayama
- T Ried
- TL Andersen
- TP Butler
- W Wang
- W Wick
- Y Kang
- Y Wang
- Y Yilmaz
- YG Park
- Publication venue
- BioMed Central
- Publication date
- Field of study
Metastasis is an enormously complex process that remains to be a major problem in the management of cancer. The fact that cancer patients might develop metastasis after years or even decades from diagnosis of the primary tumor makes the metastatic process even more complex. Over the years many hypotheses were developed to try to explain the inefficiency of the metastatic process, but none of these theories completely explains the current biological and clinical observations. In this review we summarize some of the proposed models that were developed in attempt to understand the mechanisms of tumor dissemination and colonization as well as metastatic progression
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
- A. -G. Wu
- A. C. Ma
- A. K. Au
- Abdel-Aziz A. K.
- Abdelfatah S.
- Abdellatif M.
- Abdoli A.
- Abel S.
- Abeliovich H.
- Abildgaard M. H.
- Abudu Y. P.
- Acevedo-Arozena A.
- Adamopoulos I. E.
- Adeli K.
- Adolph T. E.
- Adornetto A.
- Aflaki E.
- Agam G.
- Agarwal A.
- Aggarwal B. B.
- Agnello M.
- Agostinis P.
- Agrewala J. N.
- Agrotis A.
- Aguilar P. V.
- Ahmad S. T.
- Ahmed Z. M.
- Ahumada-Castro U.
- Aits S.
- Aizawa S.
- Akkoc Y.
- Akoumianaki T.
- Akpinar H. A.
- Al-Abd A. M.
- Al-Akra L.
- Al-Gharaibeh A.
- Alaoui-Jamali M. A.
- Alberti S.
- Alcocer-Gomez E.
- Alessandri C.
- Ali M.
- Alim Al-Bari M. A.
- Aliwaini S.
- Alizadeh J.
- Almacellas E.
- Almasan A.
- Alonso A.
- Alonso G. D.
- Altan-Bonnet N.
- Altieri D. C.
- Alvarez E. M. C.
- Alves da Costa C.
- Alves S.
- Alzaharna M. M.
- Amadio M.
- Amantini C.
- Amaral C.
- Ambrosio S.
- Amer A. O.
- Ammanathan V.
- An Z.
- Andersen S. U.
- Andrabi S. A.
- Andrade-Silva M.
- Andres A. M.
- Angelini S.
- Ann D.
- Anozie U. C.
- Ansari M. Y.
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- Antebi A.
- Anton Z.
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- Zwerschke W.
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
Multi-messenger observations of a binary neutron star merger
- Author
- Aab A
- Aartsen MG
- Abbott BP
- Abbott R
- Abbott TD
- Abbott TMC
- Abdalla H
- Abe F
- Abeysekara AU
- Abramo LR
- Abramowski A
- Abreu P
- Acernese F
- Acero F
- Ackermann M
- Ackley K
- Ackley K
- Adams C
- Adams J
- Adams SM
- Adams T
- Addesso P
- Adhikari RX
- Adya VB
- Affeldt C
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- Agarwal B
- Agathos M
- Agatsuma K
- Aggarwal N
- Aglietta M
- Agliozzo C
- Agudo I
- Aguiar OD
- Aguilar JA
- Aharonian F
- Ahlers M
- Ahrens M
- Aiello L
- Ain A
- Ajith P
- Akras S
- Al Samarai I
- Albert A
- Albert A
- Albuquerque IFM
- Albury JM
- Alcaniz JS
- Alexander KD
- Alfaro R
- Alighieri S Di Serego
- Allam S
- Allekotte I
- Allen B
- Allen G
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- Allocca A
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- Alvarez C
- Alvarez JD
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- Angus CR
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- Aoki W
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- Aramo C
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- Arcavi I
- Arceo R
- Ardid M
- Areeda JS
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- Arnaud N
- Array LWA Long Wavelength
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- Pandya H
- Paneque D
- Pang B
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- Pankova DV
- Pankow C
- Pannarale F
- Pant BC
- Panter M
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- Passaquieti R
- Passuello D
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- Pearlstone BL
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- Pekowsky L
- Pelayo R
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- Pierro V
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- Pike S
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- Piranomonte S
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- Piro AL
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- Publication venue
- 'American Astronomical Society'
- Publication date
- 01/01/2017
- Field of study
On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ~1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40+8-8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 Mo. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ~40 Mpc) less than 11 hours after the merger by the One- Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ~10 days. Following early non-detections, X-ray and radio emission were discovered at the transientâs position ~9 and ~16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta
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- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
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- Zhivotovsky B
- Zhong Q
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- Zhou Y
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- Zhou Z
- Zhou Z-Y
- Zhu B
- Zhu C
- Zhu G-Q
- Zhu H
- Zhu H
- Zhu H
- Zhu W-G
- Zhu Y
- Zhu Y
- Zhuang H
- Zhuang X
- Zientara-Rytter K
- Zimmermann CM
- Ziviani E
- Zoladek T
- Zong W-X
- Zorov DB
- Zorzano A
- Zou W
- Zou Z
- Zou Z
- Zuryn S
- Zwerschke W
- Ălvarez ĂMC
- Ăvalos Y
- Ănal G
- ĂstĂŒn S
- ÄoliÄ M
- ÄokiÄ J
- Ćœerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
DNA repair mechanisms and human cytomegalovirus (HCMV) infection
- Author
- A Ben Yehuda
- AM Skinner
- AR Evans
- AS Kulkarni
- B Gruhne
- B Karahalil
- B Smolarz
- Beata Smolarz
- C Nakai-Murakami
- CE Lilley
- CS Cobbs
- CZ Deng
- D Buck
- D Moshous
- D Samulak
- DM Wilson 3rd
- Dorota Nowakowska
- E Speir
- E Weterings
- E Xiaofei
- E Xiaofei
- E Xiaofie
- EA Fortunato
- EA Fortunato
- EK Raderschall
- FH Duong
- G Chipitsyna
- G Mathonnet
- H Costa
- HH Baydoun
- IJ Groisman
- J Bartek
- J Cinatl
- J Salsman
- J Salsman
- J Trojanek
- Jan WilczyĆski
- JH Hoeijmakers
- JM OâDowd
- JM Reitsma
- JP Savaryn
- K Dziurzynski
- K Dziurzynski
- KH Almeida
- KL Arnolds
- L Harkins
- L Jia
- L Soroceanu
- LE Gamadia
- M Gaspar
- M Michaelis
- M Michaelis
- M Miller-Kittler
- M Nystad
- M Samanta
- MD Weitzman
- MH Luo
- MS Chaurushiya
- MS Chee
- N Stern-Ginossar
- NJ Moorman
- P Hsieh
- P Karran
- PC Hanawalt
- PM Fliss
- S AbuBakar
- S Krichevsky
- S Neri
- S Pal
- S Prost
- S Terhune
- S Tonegawa
- SA Becker
- SE Polo
- SK Weller
- SM Philpott
- SP Jackson
- SP Jackson
- SS Durkin
- T Albrecht
- T Haaf
- T Helleday
- T Ranneberg-Nilsen
- VK Siew
- X Liang
- Y Nishiyama
- Y Shen
- Y Sun
- Z Qian
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
- âŠ