Imperfect nesting and Peierls instability for a two-dimensional tight-binding model

Based on a half-filled two-dimensional tight-binding model with nearest-neighbour and next nearest-neighbour hopping the effect of imperfect Fermi surface nesting on the Peierls instability is studied at zero temperature. Two dimerization patterns corresponding to a phonon vector $(\pi, \pi)$ are considered. It is found that the Peierls instability will be suppressed with an increase of next nearest-neighbour hopping which characterizes the nesting deviation. First and second order transitions to a homogeneous state are possible. The competition between the two dimerized states is discussed.Comment: 17 pages, 10 eps figure

Magnetotelluric image of the fluid cycle in the Costa Rican subduction zone

Fluids entering the subduction zone are a key factor in the subduction process. They determine the onset of melting, weakening and changes in the dynamics and thermal structure of subduction zones and trigger earthquakes when being released from the subducting plate in a series of metamorphic processes. However, the amount of water carried into the subduction zone and its distribution are not well constrained by existing data and are subject of vigorous current research in SFB574 (Volatiles and Fluids in Subduction Zones: Climate Feedback and Trigger Mechanisms for Natural Disasters). Electromagnetic methods like magnetotellurics have been used widely to recognize fluid release and melt production through enhanced electrical conductivities. Here we present an image of the hydration and dehydration cycle down to 120 km depth in one setting derived by an onshore-offshore transect of magnetotelluric soundings in Costa Rica. An electrically conductive zone in the incoming plate outer rise is associated with sea water penetrating down extensional faults and cracks into the upper mantle possibly causing serpentinization. Along the downward subducting plate distinct conductive anomalies identify fluids from dehydration of sediments, crust and mantle. A conductivity anomaly at a depth of approx. 12 km and at a distance of 65 km from the trench is associated with a first major dehydration reaction of minerally-bound water. This is of importance in the context of mid-slope fluid seeps which are thought to significantly contribute to the recycling of minerally-bound water. The position of the conductivity anomaly correlates with geochemical and seismic evidence stating that mid-slope fluids are originated at >=12 km depth before rising up through deep faults to the seeps. The conductivity anomaly is therefore associated with a fluid accumulation feeding the mid-slope seeps. Another fluid accumulation is revealed by a conductivity anomaly at 20-30 km depth and a distance of approximately 30 km seaward from the volcanic arc. This lower crustal fluid accumulation could likely be caused by trapping of fluids released due to de-serpentinization processes or due to other mineral dehydration processes. While we are at the moment not able to attribute one specific process causing the anomaly based on electromagnetic data alone, this feature is however of fundamental importance. A comparison with other electromagnetic studies from subduction zones around the world reveal that such a conductivity anomaly is a global feature suggesting the presence of a global fluid sink. Based on very simplified assumptions we are able derive rough estimates for the amount of water being stored in the overriding plate. Relating seismic evidence as well as petrological results collected in the multi-disciplinary study on the Costa Rican subduction zone we introduce budget estimations for the water cycle in the subduction zone

Geschichte und Gegenwart des kastilisch-deutschen Sprachkontakts in der Kolonie Pozuzo, Peru

Die Diplomarbeit behandelt den kastilisch-deutschen Sprachkontakt in der peruanischen Kolonie Pozuzo, welche 1859 von größtenteils Tiroler und Rheinländer Emigranten gegründet wurde. Eingebettet in die historische Thematik der Entwicklung von Pozuzo wird in der Arbeit die soziolinguistische Frage nach der Sprachentwicklung in der österreichisch-deutschen Kolonie erarbeitet. Es wird darauf eingegangen, wie der kastilisch-deutsche Sprachkontakt verlief, wie es um das Sprachbewusstsein der Pozuciner steht und wie die aktuelle Sprachsituation in der Kolonie aussieht. Für die Ausarbeitung der Forschungsfrage konnten während einem Aufenthalt in der Kolonie unter Anwendung der qualitativen Methode (Einzelinterviews) soziolinguistische Ergebnisse in Bezug auf die sprachliche Entwicklung, den Sprachkontakt und das Sprachbewusstsein erlangt werden. Zudem wird einschlägige Literatur über die Kolonie als Grundlage verwendet, um die Forschungsergebnisse in ihrem Kontext situieren zu können. Aus verschiedenen Gründen kam es in den letzten 150 Jahren zur Dominanz der kastilischen Landessprache und zu einem Rückgang des deutschen Idioms. Lehnbildungen, Interferenzen und Codewechsel können als Folgen des Sprachkontakts beobachtet werden. Gegenwärtig wird der deutsche Kolonistendialekt nur mehr von einigen Personen der älteren Generation gesprochen. Auch wenn der Sprachverlust des Pozuzo-Tirolerischen nicht mehr rückgängig zu machen ist, stieg sein Prestigewert in den letzten Jahren durch den Kontakt zu Österreich und den aufkommenden Tourismus jedoch wieder deutlich an. Mit den letzten Sprechern des deutschen Dialekts wird wohl auch der alte Siedlerdialekt die Kolonie verlassen. Es ist jedoch wahrscheinlich, dass Spuren der deutschen Sprache in Form von Lehnwörtern und Interferenzen auch noch nach dem Sprachinseltod zu entdecken sein werden

Immunopathologic Features of Allergic Contact Dermatitis in Humans: Participation of Plasmacytoid Dendritic Cells in the Pathogenesis of the Disease?

Contrary to our abundant knowledge about the sensitization phase of human contact hypersensitivity, little is known about the cell types orchestrating the effector phase. In order to address this issue, we phenotypically analyzed biopsies from 72 h epicutaneous patch test reactions (n=10) and normal human skin (n=5) for the presence of various leukocyte differentiation antigens. The inflammatory infiltrate was dominated by CD3+/CD4+ T cells with approximately 30% of the cells coexpressing CD25 and CTLA-4, a phenotype consistent with either activated effector or regulatory T cells. In our search for professional antigen-presenting cells, we were surprised to find not only sizeable numbers of CD1a+ dendritic cells and CD1c+ dendritic cells, but also of CD123+, CD45RA+, BDCA-2+, CLA+, and CD62L+ plasmacytoid dendritic cells. Although virtually absent in normal human skin, these cells were detectable already 6 h after hapten challenge and were often found in close proximity to CD56+ natural killer cells, indicative of a functional interaction between these cell types. The detailed knowledge of the cellular composition of the inflammatory infiltrate in allergic contact dermatitis and its kinetics should form the basis for the investigation of the immunologic and molecular events operative in the perpetuation and resolution of the eczematous response

Magnetotelluric image of the fluid cycle in the Costa Rican subduction zone

Fluids entering the subduction zone play a key role in the subduction process. They cause changes in the dynamics and thermal structure of the subduction zone1, and trigger earthquakes when released from the subducting plate during metamorphism. Fluids are delivered to the subduction zone by the oceanic crust and also enter as the oceanic plate bends downwards at the plate boundary. However, the amount of fluids entering subduction zones is not matched by that leaving through volcanic emissions4 or transfer to the deep mantle, implying possible storage of fluids in the crust. Here we use magnetotelluric data to map the entire hydration and dehydration cycle of the Costa Rican subduction zone to 120 km depth. Along the incoming plate bend, we detect a conductivity anomaly that we interpret as sea water penetrating down extensional faults and cracks into the upper mantle. Along the subducting plate interface we document the dehydration of sediments, the crust and mantle. We identify an accumulation of fluids at ~20–30 km depth at a distance of 30 km seaward from the volcanic arc. Comparison with other subduction zones5–14 indicates that such fluid accumulation is a global phenomenon. Although we are unable to test whether these fluid reservoirs grow with time, we suggest that they can account for some of the missing outflow of fluid at subduction zones

Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7‐related osteopetrosis

Human induced pluripotent stem cells (hiPSCs) hold great potential for modeling human diseases and the development of innovative therapeutic approaches. Here, we report on a novel, simplified differentiation method for forming functional osteoclasts from hiPSCs. The three-step protocol starts with embryoid body formation, followed by hematopoietic specification, and finally osteoclast differentiation. We observed continuous production of monocyte-like cells over a period of up to 9 weeks, generating sufficient material for several osteoclast differentiations. The analysis of stage-specific gene and surface marker expression proved mesodermal priming, the presence of monocyte-like cells, and of terminally differentiated multinucleated osteoclasts, able to form resorption pits and trenches on bone and dentine in vitro. In comparison to peripheral blood mononuclear cell (PBMC)-derived osteoclasts hiPSC-derived osteoclasts were larger and contained a higher number of nuclei. Detailed functional studies on the resorption behavior of hiPSC-osteoclasts indicated a trend towards forming more trenches than pits and an increase in pseudoresorption. We used hiPSCs from an autosomal recessive osteopetrosis (ARO) patient (BIHi002-A, ARO hiPSCs) with compound heterozygous missense mutations p.(G292E) and p.(R403Q) in CLCN7, coding for the Cl-/H+-exchanger ClC-7, for functional investigations. The patient's leading clinical feature was a brain malformation due to defective neuronal migration. Mutant ClC-7 displayed residual expression and retained lysosomal co-localization with OSTM1, the gene coding for the osteopetrosis-associated transmembrane protein 1, but only ClC-7 harboring the mutation p.(R403Q) gave strongly reduced ion currents. An increased autophagic flux in spite of unchanged lysosomal pH was evident in undifferentiated ARO hiPSCs. ARO hiPSC-derived osteoclasts showed an increased size compared to hiPSCs of healthy donors. They were not able to resorb bone, underlining a loss-of-function effect of the mutations. In summary, we developed a highly reproducible, straightforward hiPSC-osteoclast differentiation protocol. We demonstrated that osteoclasts differentiated from ARO hiPSCs can be used as a disease model for ARO and potentially also other osteoclast-related diseases. (c) 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR)

Genetic Association for Renal Traits among Participants of African Ancestry Reveals New Loci for Renal Function

Chronic kidney disease (CKD) is an increasing global public health concern, particularly among populations of African ancestry. We performed an interrogation of known renal loci, genome-wide association (GWA), and IBC candidate-gene SNP association analyses in African Americans from the CARe Renal Consortium. In up to 8,110 participants, we performed meta-analyses of GWA and IBC array data for estimated glomerular filtration rate (eGFR), CKD (eGFR <60 mL/min/1.73 m2), urinary albumin-to-creatinine ratio (UACR), and microalbuminuria (UACR >30 mg/g) and interrogated the 250 kb flanking region around 24 SNPs previously identified in European Ancestry renal GWAS analyses. Findings were replicated in up to 4,358 African Americans. To assess function, individually identified genes were knocked down in zebrafish embryos by morpholino antisense oligonucleotides. Expression of kidney-specific genes was assessed by in situ hybridization, and glomerular filtration was evaluated by dextran clearance. Overall, 23 of 24 previously identified SNPs had direction-consistent associations with eGFR in African Americans, 2 of which achieved nominal significance (UMOD, PIP5K1B). Interrogation of the flanking regions uncovered 24 new index SNPs in African Americans, 12 of which were replicated (UMOD, ANXA9, GCKR, TFDP2, DAB2, VEGFA, ATXN2, GATM, SLC22A2, TMEM60, SLC6A13, and BCAS3). In addition, we identified 3 suggestive loci at DOK6 (p-value = 5.3×10−7) and FNDC1 (p-value = 3.0×10−7) for UACR, and KCNQ1 with eGFR (p = 3.6×10−6). Morpholino knockdown of kcnq1 in the zebrafish resulted in abnormal kidney development and filtration capacity. We identified several SNPs in association with eGFR in African Ancestry individuals, as well as 3 suggestive loci for UACR and eGFR. Functional genetic studies support a role for kcnq1 in glomerular development in zebrafish

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements