Ask a clearer question, get a better answer.

Many undergraduate students struggle to engage with higher order skills such as evaluation and synthesis in written assignments, either because they do not understand that these are the aim of written assessment or because these critical thinking skills require more effort than writing a descriptive essay. Here, we report that students who attended a freely available workshop, in which they were coached to pose a question in the title of their assignment and then use their essay to answer that question, obtained higher marks for their essay than those who did not attend. We demonstrate that this is not a result of latent academic ability amongst students who chose to attend our workshops and suggest this increase in marks was a result of greater engagement with ‘critical thinking’ skills, which are essential for upper 2:1 and 1st class grades. The tutoring method we used holds two particular advantages: First, we allow students to pick their own topics of interest, which increases ownership of learning, which is associated with motivation and engagement in ‘difficult’ tasks. Second, this method integrates the development of ‘inquisitiveness’ and critical thinking into subject specific learning, which is thought to be more productive than trying to develop these skills in isolation

The Physiological Response of sub-Arctic Lichens to Their Abiotic Environment

The effect of environmental factors on metabolic processes of sample thalli, of four sub-Arctic lichen species from Abisko, Sweden (68.37˚N, 18.69˚E). Nephroma arcticum, Cladina mitis, C. stellaris and C. rangiferina, which are found in contrasting niche habitats within the same ecosystem, have been studied. An oxygen electrode apparatus has been used to study the effects of air temperature, thallus irradiance intensity and climatic regime during thallus storage, on the rate of net photosynthesis and dark respiration of sample thalli, in a series of studies on the four lichen species named above. Environmental temperature and light intensity both significantly affected the rate of thallus net photosynthesis (NP) in samples of all four species. The mean rate of thallus NP was significantly depressed at 17⁰C in all four species due to an exponential increase in respiratory rate with rising temperature. Maximal NP was achieved at 5⁰C in N. arcticum, 10⁰C in C. rangiferina, and NP at 5 & 10⁰C were not significantly different in C. mitis and C. stellaris. Optimum light intensity for photosynthesis was 340 µmol m-2 s-1 in all four species; however, temperature significantly increased the light compensation point of photosynthesis. Furthermore, mean light compensation points were greater in the more shaded adapted species N. arcticum and C. mitis; suggesting, as found in vascular plants, lichens exhibit photosynthetic characteristics adapted to their abiotic environment. C. mitis thalli did not provide any evidence of plastic adaptation to temperature over a six day storage period; whereas, N. arcticum samples exhibited increased NP after six days at 10 & 15⁰C compared to non-acclimatized samples. Prolonged storage at 10⁰C appeared to be detrimental to samples of C. stellaris. Responses to temperature and light intensity were similar to those found, in similar species, in photosynthetic rate investigations utilizing IRGA apparatus; validating the use of O2 electrode experiments using small samples of thallus tissue, as used in this investigation. The response of species, within this study, to environmental factors was slightly different to those obtained in similar studies for North American populations, which suggests that lichens may exhibit some within-species genetic variation between lichen populations from different continents. Analysis of moisture availability, from past climate-data of the region from which the lichen populations are found, suggests that the growth season of sub-Arctic lichens is very different to that of vascular plants that are within the same ecosystem. Future warming may cause longer, wetter Arctic Autumns and Springs, which would benefit lichen biomass, but environmental warming would suppress lichen growth and promote vascular plant productivity.\u

I am a scientist: Overcoming biased assumptions around diversity in science through explicit representation of scientists in lectures

The lack of diversity in Science, Technology, Engineering, and Mathematics (STEM) is a significant issue for the sector. Many organisations and educators have identified lack of representation of historically marginalised groups within teaching materials as a potential barrier to students feeling that a Science, Technology, Engineering, and Mathematics (STEM) career is something that they can aspire to. A key barrier to addressing the issue is providing accessible and effective evidence-based approaches for educators to implement. In this study, we explore the potential for adapting presentation slides within lectures to 'humanise' the scientists involved, presenting their full names and photographs alongside a Harvard style reference. The intervention stems from an initial assumption that many formal scientific referencing systems are demographic-neutral and exacerbate prevailing perceptions that STEM is not diverse. We adopt a questionnaire based methodology surveying 161 bioscience undergraduates and postgraduates at a UK civic university. We first establish that students project assumptions about the gender, location, and ethnicity of the author of a hypothetical reference, with over 50% of students assuming they are male and Western. We then explore what students think of the humanised slide design, concluding that many students see it as good pedagogical practice with some students positively changing their perceptions about diversity in science. We were unable to compare responses by participant ethnic group, but find preliminary evidence that female and non-binary students are more likely to see this as good pedagogical practice, perhaps reflecting white male fragility in being exposed to initiatives designed to highlight diversity. We conclude that humanised powerpoint slides are a potentially effective tool to highlight diversity of scientists within existing research-led teaching, but highlight that this is only a small intervention that needs to sit alongside more substantive work to address the lack of diversity in STEM

From Individuals to Ecosystems: A Study of the Temporal and Spatial Variation in Ecological Network Structure

Ecological network theory has developed from studies of static, binary trophic relationships to the analysis of quantitative, dynamic communities consisting of multiple link-types. Particularly, work has focused on the dynamic nature of ecological networks, which maintains stability in complex communities. However, there are few in situ network-level studies of the determinants of temporal and spatial variation in community structure. This thesis utilises data from a 10-year study of a host-parasitoid network and a collaborative study in an applied ecological setting to identify individual level factors important to network structure. The work aims towards an empirical, predictive framework linking adaptive foraging behaviour to ecological network structure. The results show that condition-dependent foraging behaviours structure host-parasitoid networks. The realised niches of the studied parasitoid species were generally biased towards larger host species and condition-dependent sex ratio allocation increased the likelihood that females would eclose from relatively larger hosts and males from relatively smaller hosts, which resulted in sex ratios deviating from Hamiltonian (50:50) predictions. Further, both of these aspects of behaviour are plastic, where parasitoid behaviour responded to environmental heterogeneity. Particularly, host preference behaviour conformed to an egg-/time-limitation framework, where the size dependency of the behaviour is greater when individuals have a greater likelihood of being egg-limited. Both the size-dependency and the plasticity of these behaviours differed significantly between secondary parasitoid species. This species identity effect interacted with landscape heterogeneity, which may explain some inter- and intra-specific variation in network structure. With respect to applied ecology, the results show that the benefits of natural vegetation for pest control are dependent upon the dispersal capabilities and the diet breadth of the pest and its natural enemies. The findings are evaluated towards a predictive framework for understanding the effects of future climate change on community structure and stability. We consider this framework in terms of applied ecology, particularly pest control ecosystem services provided by natural vegetation in an agricultural environment. The synergistic nature of the multiple determinants of network structure found in this thesis suggest that future studies should focus on the whole network, which is not necessarily the sum of its parts

Developing undergraduate practical skills and independence with ‘at home practical kits’

The Covid-19 pandemic posed significant challenges for practical teaching within the sciences. While many instructors adopted innovative alternatives to conventional practicals, many relied on digital approaches that did not give students hands-on experience. In this study we evaluate the use of ‘at home’ practical kits used in first year physics and biology teaching at a UK university as an alternative to laboratory classes. In particular we focus on the enforced independence over time, space and help-seeking inherent in the at-home model as a driver of student learning and confidence. Students reported the kits encouraged independence, problem solving and self-reliance. Students associated the at-home practical kits with higher level cognitive skills as defined by Bloom’s revised taxonomy. While most students enjoyed using the kits, those who did not enjoy them tended to have higher previous experience of practical work before university. Students saw potential value in the kits after the pandemic, so could be an alternative or supplement to in-person practicals. We recommend that practical organisers use our findings around the development of student self-reliance to reconsider practical design and incorporate more opportunities for students to solve problems independently to increase effectiveness of practical teaching

Student employability enhancement through fieldwork: purposefully integrated or a beneficial side effect?

Fieldwork provides opportunities for students to develop employability-enhancing transferable skills as well as technical, discipline-specific skills and disciplinary knowledge. However, the extent to which staff purposely plan transferable skills outcomes of field courses, and, therefore, whether they are communicated to students is unknown. We investigated whether staff intentionally plan transferable skills development opportunities into fieldwork by interviewing academic staff responsible for planning and leading residential field courses at a UK university. We also conducted a thematic analysis of associated module specifications and teaching materials to understand whether transferable skills were signposted to colleagues and students. Our findings show that although most staff recognise that their field courses help students to develop transferable skills, staff awareness of skills and professional development outcomes is narrowly focused on technical skills and discipline-related careers. Furthermore, those transferable skills outcomes that staff are aware of are not fully translated into module specifications and infrequently signposted to students via teaching materials. These findings suggest that transferable skills form a hidden curriculum of fieldwork. To maximise the employability benefits of fieldwork, we recommend that all skills should be signposted to students both during field course teaching and also via the associated teaching materials

Prenatal phenotyping: A community effort to enhance the Human Phenotype Ontology.

Technological advances in both genome sequencing and prenatal imaging are increasing our ability to accurately recognize and diagnose Mendelian conditions prenatally. Phenotype-driven early genetic diagnosis of fetal genetic disease can help to strategize treatment options and clinical preventive measures during the perinatal period, to plan in utero therapies, and to inform parental decision-making. Fetal phenotypes of genetic diseases are often unique and at present are not well understood; more comprehensive knowledge about prenatal phenotypes and computational resources have an enormous potential to improve diagnostics and translational research. The Human Phenotype Ontology (HPO) has been widely used to support diagnostics and translational research in human genetics. To better support prenatal usage, the HPO consortium conducted a series of workshops with a group of domain experts in a variety of medical specialties, diagnostic techniques, as well as diseases and phenotypes related to prenatal medicine, including perinatal pathology, musculoskeletal anomalies, neurology, medical genetics, hydrops fetalis, craniofacial malformations, cardiology, neonatal-perinatal medicine, fetal medicine, placental pathology, prenatal imaging, and bioinformatics. We expanded the representation of prenatal phenotypes in HPO by adding 95 new phenotype terms under the Abnormality of prenatal development or birth (HP:0001197) grouping term, and revised definitions, synonyms, and disease annotations for most of the 152 terms that existed before the beginning of this effort. The expansion of prenatal phenotypes in HPO will support phenotype-driven prenatal exome and genome sequencing for precision genetic diagnostics of rare diseases to support prenatal care

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Discovery and validation of biomarkers to guide clinical management of pneumonia in African children.

BACKGROUND: Pneumonia is the leading cause of death in children globally. Clinical algorithms remain suboptimal for distinguishing severe pneumonia from other causes of respiratory distress such as malaria or distinguishing bacterial pneumonia and pneumonia from others causes, such as viruses. Molecular tools could improve diagnosis and management. METHODS: We conducted a mass spectrometry-based proteomic study to identify and validate markers of severity in 390 Gambian children with pneumonia (n = 204) and age-, sex-, and neighborhood-matched controls (n = 186). Independent validation was conducted in 293 Kenyan children with respiratory distress (238 with pneumonia, 41 with Plasmodium falciparum malaria, and 14 with both). Predictive value was estimated by the area under the receiver operating characteristic curve (AUC). RESULTS: Lipocalin 2 (Lpc-2) was the best protein biomarker of severe pneumonia (AUC, 0.71 [95% confidence interval, .64-.79]) and highly predictive of bacteremia (78% [64%-92%]), pneumococcal bacteremia (84% [71%-98%]), and "probable bacterial etiology" (91% [84%-98%]). These results were validated in Kenyan children with severe malaria and respiratory distress who also met the World Health Organization definition of pneumonia. The combination of Lpc-2 and haptoglobin distinguished bacterial versus malaria origin of respiratory distress with high sensitivity and specificity in Gambian children (AUC, 99% [95% confidence interval, 99%-100%]) and Kenyan children (82% [74%-91%]). CONCLUSIONS: Lpc-2 and haptoglobin can help discriminate the etiology of clinically defined pneumonia and could be used to improve clinical management. These biomarkers should be further evaluated in prospective clinical studies

Crop pests and predators exhibit inconsistent responses to surrounding landscape composition

The idea that noncrop habitat enhances pest control and represents a win–win opportunity to conserve biodiversity and bolster yields has emerged as an agroecological paradigm. However, while noncrop habitat in landscapes surrounding farms sometimes benefits pest predators, natural enemy responses remain heterogeneous across studies and effects on pests are inconclusive. The observed heterogeneity in species responses to noncrop habitat may be biological in origin or could result from variation in how habitat and biocontrol are measured. Here, we use a pest-control database encompassing 132 studies and 6,759 sites worldwide to model natural enemy and pest abundances, predation rates, and crop damage as a function of landscape composition. Our results showed that although landscape composition explained significant variation within studies, pest and enemy abundances, predation rates, crop damage, and yields each exhibited different responses across studies, sometimes increasing and sometimes decreasing in landscapes with more noncrop habitat but overall showing no consistent trend. Thus, models that used landscape-composition variables to predict pest-control dynamics demonstrated little potential to explain variation across studies, though prediction did improve when comparing studies with similar crop and landscape features. Overall, our work shows that surrounding noncrop habitat does not consistently improve pest management, meaning habitat conservation may bolster production in some systems and depress yields in others. Future efforts to develop tools that inform farmers when habitat conservation truly represents a win–win would benefit from increased understanding of how landscape effects are modulated by local farm management and the biology of pests and their enemies