Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function

<p>Abstract</p> <p>Background</p> <p>Previous studies demonstrated <it>Ganoderma lucidum </it>polysaccharides (GL-PS), a form of bioactive β-glucan can stimulate the maturation of monocyte-derived dendritic cells (DC). The question of how leukemic cells especially in monocytic lineage respond to GL-PS stimuli remains unclear.</p> <p>Results</p> <p>In this study, we used <it>in vitro</it> culture model with leukemic monocytic cell-lines THP-1 and U937 as monocytic effectors cells for proliferation responses and DCs induction. We treated the THP-1 and U937 cells with purified GL-PS (100 μg/mL) or GL-PS with GM-CSF/IL-4. GL-PS alone induced proliferative response on both THP-1 and U937 cells but only THP-1 transformed into typical DC morphology when stimulated with GL-PS plus GM-CSF/IL-4. The transformed THP-1 DCs had significant increase expression of HLA-DR, CD40, CD80 and CD86 though not as high as the extent of normal monocyte-derived DCs. They had similar antigen-uptake ability as the normal monocyte-derived DCs positive control. However, their potency in inducing allogeneic T cell proliferation was also less than that of normal monocyte-derived DCs.</p> <p>Conclusion</p> <p>Our findings suggested that GL-PS could induce selected monocytic leukemic cell differentiation into DCs with immuno-stimulatory function. The possible clinical impact of using this commonly used medicinal mushroom in patients with monocytic leukemia (AML-M4 and M5) deserved further investigation.</p

Multi-messenger observations of a binary neutron star merger

On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ~1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40+8-8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 Mo. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ~40 Mpc) less than 11 hours after the merger by the One- Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ~10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position ~9 and ~16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta

Disseminated mediastinal carcinoma with chromosomal translocation (15;19): A distinctive clinicopathologic syndrome

Background. A case of disseminated mediastinal carcinoma probably of thymic origin is reported in a 5-year-old boy with an anterior mediastinal mass associated with superior vena cava obstruction, tracheal deviation, right malignant pleural effusion, and evidence of bone metastasis. The diagnosis was based on findings of radiologic localization, light and electron microscopic study, and immunohistochemistry. The patient received combination chemotherapy but died of progressive disease. Methods. Cytogenic study on the pleural fluid was attempted, and a literature search for similar chromosomal aberration was performed. Results. Cytogenetic study of tumor cells from the pleural fluid revealed a clonal chromosomal abnormality of t(15;19)(q12;p13.1). Two patients with similar disease were reported in the English literature; their clinical courses, immunohistochemical findings, and t(15;19) were almost identical to those of the patient reported in this study. Conclusion. The authors suggest that this translocation may be specific to thymic carcinoma and may indicate a particularly aggressive form of the disease. Carcinomas are rare in children, so the chromosomal translocation may serve as a helpful marker for the diagnosis.link_to_subscribed_fulltex

Ligand binding and structural properties of segments of GABA(A) receptor alpha(1) subunit overexpressed in Escherichia coli

The gamma-aminobutyric acid, type A (GABA(A)), receptor is the target for numerous therapeutic compounds. In the present study, the Gln(28)-Leu(296), Gln(28)-Arg(276), Gln(28)-Arg(248), and Gln(28)-Glu(165) (numbering of bovine precursor protein) segments of its alpha(1) subunit were overexpressed in Escherichia coli, along with Cys(166)-Leu(296) produced previously, for structural analysis by circular dichroism and ligand binding studies by fluorescence spectroscopy, Results showed that the protein segments were rich in beta-sheet structures. Binding of the fluorescent benzodiazepine Bodipy-FL Ro-1986 was evident from fluorescence resonance energy transfer and fluorescence anisotropy measurements. The binding affinity was in the micromolar range. The binding was attributable more to Cys(166)-Leu(296) than 60 Gln(28)-Glu(165) and was inhibited by known central benzodiazepine site ligands, Three point mutations, Y187A, T234A, and Y237A, were found to perturb protein secondary structures. Studies with the single Trp mutants W198Y and W273Y indicated that Trp(273) was closer to the binding site than Trp(198)

Liquid-liquid equilibrium of the [4-MBP][BF4]-NaCl-H2O systems at T=293.15, 303.15, 313.15, and 323.15 K: Experimentation and correlation

The binodal data and liquid-liquid equilibrium of a 1-butyl-4-methylpyridinium tetrafluoroborate ([4-MBP][BF4]) + sodium chloride (NaCl) + H2O system were examined at T = 298.15, 303.15, 313.15, and 323.15 K. Moreover, the binodal curves were fitted to the Merchuk equation and another two four-fitting parameters empirical expressions, and the Othmer-Tobias, Bancroft and Setschenow-type equations were used to correlate the tie-line data. Good agreement was obtained between the experimental binodal data and the tie-line data. The results provide a theoretical basis for the extraction, separation, and purification of [4-MBP][BF4]-NaCl-H2O systems.</p

Large-scale synthesis and excellent microwave absorption of highly-dispersed Fe nanoparticles via RF thermal plasma

Spherical Fe nanoparticles (FeNPs) with diameter of similar to 100 nm were prepared by evaporating carbonyl iron particles (CIPs) using RF thermal plasma in a large scale. The FeNPs presented smooth surface, good dispersity and excellent antioxidation performance, which could be homogeneously distributed in paraffin with a particle loading as high as 90 wt%. In comparison to the raw CIPs, the FeNPs showed enhanced resonance characteristics in both permittivity and permeability due to the particle size effect. It was found that the FeNPs/paraffin composite exhibited excellent microwave absorption in terms of small matching thickness and broad bandwidth. The percentage bandwidth with reflection loss <=-10 dB for the Fe nanocomposite is larger than that for the carbonyl iron composite at any specified thickness in the range of 1.0. similar to 2.5 mm. This work demonstrates that the synthesized FeNPs hold great promise for developing thin-layered absorbers with excellent microwave absorbing performance