18 research outputs found
IL-2 production by dendritic cells is not critical for the activation of cognate and innate effectors in draining lymph nodes
Toll-like receptor (TLR) polymorphisms in African children: Common TLR-4 variants predispose to severe malaria
The use of nanoscale fluorescence microscopic to decipher cell wall modifications during fungal penetration
Plant diseases are one of the most studied subjects in the field of plant science due to their
impact on crop yield and food security. Our increased understanding of plant–pathogen
interactions was mainly driven by the development of new techniques that facilitated
analyses on a subcellular and molecular level. The development of labeling technologies,
which allowed the visualization and localization of cellular structures and proteins in live
cell imaging, promoted the use of fluorescence and laser-scanning microscopy in the field
of plant–pathogen interactions. Recent advances in new microscopic technologies opened
their application in plant science and in the investigation of plant diseases. In this regard,
in planta Förster/Fluorescence resonance energy transfer has demonstrated to facilitate
the measurement of protein–protein interactions within the living tissue, supporting the
analysis of regulatory pathways involved in plant immunity and putative host–pathogen
interactions on a nanoscale level. Localization microscopy, an emerging, non-invasive
microscopic technology, will allow investigations with a nanoscale resolution leading to
new possibilities in the understanding of molecular processes
Cracking the elusive alignment hypothesis: the microtubule–cellulose synthase nexus unraveled
Genetic studies of abdominal MRI data identify genes regulating hepcidin as major determinants of liver iron concentration
Genetic analysis of blood molecular phenotypes reveals common properties in the regulatory networks affecting complex traits
We evaluate the shared genetic regulation of mRNA molecules, proteins and metabolites derived from whole blood from 3029 human donors. We find abundant allelic heterogeneity, where multiple variants regulate a particular molecular phenotype, and pleiotropy, where a single variant associates with multiple molecular phenotypes over multiple genomic regions. The highest proportion of share genetic regulation is detected between gene expression and proteins (66.6%), with a further median shared genetic associations across 49 different tissues of 78.3% and 62.4% between plasma proteins and gene expression. We represent the genetic and molecular associations in networks including 2828 known GWAS variants, showing that GWAS variants are more often connected to gene expression in trans than other molecular phenotypes in the network. Our work provides a roadmap to understanding molecular networks and deriving the underlying mechanism of action of GWAS variants using different molecular phenotypes in an accessible tissue. Perioperative Medicine: Efficacy, Safety and Outcome (Anesthesiology/Intensive Care