124 research outputs found
A map of OMC-1 in CO 9-8
The distribution of 12C16O J=9-8 (1.037 THz) emission has been mapped in
OMC-1 at 35 points with 84" resolution. This is the first map of this source in
this transition and only the second velocity-resolved ground-based observation
of a line in the terahertz frequency band. There is emission present at all
points in the map, a region roughly 4' by 6' in size, with peak antenna
temperature dropping only near the edges. Away from the Orion KL outflow, the
velocity structure suggests that most of the emission comes from the OMC-1
photon-dominated region, with a typical linewidthof 3-6 km/s. Large velocity
gradient modeling of the emission in J=9-8 and six lower transitions suggests
that the lines originate in regions with temperatures around 120 K and
densities of at least 10^(3.5) cm^(-3) near theta^(1) C Ori and at the Orion
Bar, and from 70 K gas at around 10^(4) cm^(-3) southeast and west of the bar.
These observations are among the first made with the 0.8 m Smithsonian
Astrophysical Observatory Receiver Lab Telescope, a new instrument designed to
observe at frequencies above 1 THz from an extremely high and dry site in
northern Chile.Comment: Minor changes to references, text to match ApJ versio
Water and methanol in low-mass protostellar outflows: gas-phase synthesis, ice sputtering and destruction
Water in outflows from protostars originates either as a result of gas-phase synthesis from atomic oxygen at T ≳ 200 K, or from sputtered ice mantles containing water ice. We aim to quantify the contribution of the two mechanisms that lead to water in outflows, by comparing observations of gas-phase water to methanol (a grain surface product) towards three low-mass protostars in NGC 1333. In doing so, we also quantify the amount of methanol destroyed in outflows. To do this, we make use of James Clerk Maxwell Telescope and Herschel-Heterodyne Instrument for the Far-Infrared data of H2O, CH3OH and CO emission lines and compare them to RADEX non-local thermodynamic equilibrium excitation simulations. We find up to one order of magnitude decrease in the column density ratio of CH3OH over H2O as the velocity increases in the line wings up to ∼15 km s−1. An independent decrease in X(CH3OH) with respect to CO of up to one order of magnitude is also found in these objects. We conclude that gas-phase formation of H2O must be active at high velocities (above 10 km s−1 relative to the source velocity) to re-form the water destroyed during sputtering. In addition, the transition from sputtered water at low velocities to form water at high velocities must be gradual. We place an upper limit of two orders of magnitude on the destruction of methanol by sputtering effects
The N2D+/N2H+ ratio as an evolutionary tracer of Class 0 protostars
Deuterated ions are abundant in cold (T=10 K), dense (n=10^5 cm^-3) regions,
in which CO is frozen out onto dust grains. In such environments, the deuterium
fractionation of such ions can exceed the elemental abundance ratio of D/H by a
factor of 10^4. In this paper we use the deuterium fractionation to investigate
the evolutionary state of Class 0 protostars. In a sample of 20 protostellar
objects, we found a clear correlation between the N2D+/N2H+ ratio and
evolutionary tracers. As expected, the coolest, i.e. the youngest, objects show
the largest deuterium fractionation. Furthermore, we find that sources with a
high N2D+/N2H+ ratio show clear indication for infall.Comment: 19 pages, 12 figures, accepted by A&
Molecular excitation in the Interstellar Medium: recent advances in collisional, radiative and chemical processes
We review the different excitation processes in the interstellar mediumComment: Accepted in Chem. Re
Abnormal molecular signatures of inflammation, energy metabolism, and vesicle biology in human Huntington disease peripheral tissues
BACKGROUND: A major challenge in neurodegenerative diseases concerns identifying biological disease signatures that track with disease progression or respond to an intervention. Several clinical trials in Huntington disease (HD), an inherited, progressive neurodegenerative disease, are currently ongoing. Therefore, we examine whether peripheral tissues can serve as a source of readily accessible biological signatures at the RNA and protein level in HD patients. RESULTS: We generate large, high-quality human datasets from skeletal muscle, skin and adipose tissue to probe molecular changes in human premanifest and early manifest HD patients—those most likely involved in clinical trials. The analysis of the transcriptomics and proteomics data shows robust, stage-dependent dysregulation. Gene ontology analysis confirms the involvement of inflammation and energy metabolism in peripheral HD pathogenesis. Furthermore, we observe changes in the homeostasis of extracellular vesicles, where we find consistent changes of genes and proteins involved in this process. In-depth single nucleotide polymorphism data across the HTT gene are derived from the generated primary cell lines. CONCLUSIONS: Our ‘omics data document the involvement of inflammation, energy metabolism, and extracellular vesicle homeostasis. This demonstrates the potential to identify biological signatures from peripheral tissues in HD suitable as biomarkers in clinical trials. The generated data, complemented by the primary cell lines established from peripheral tissues, and a large panel of iPSC lines that can serve as human models of HD are a valuable and unique resource to advance the current understanding of molecular mechanisms driving HD pathogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-022-02752-5
Prestellar and protostellar cores in Ori B9
The aims of this study are to determine the properties and spatial
distribution of dense cores in Ori B9, and to estimate their ages and dynamical
timescales. The cloud was mapped in the 870 micron continuum with APEX/LABOCA,
and selected positions were observed in the lines of N2H+ and N2D+ using
IRAM-30m. These were used together with our previous H2D+ observations.
Moreover, archival FIR Spitzer/MIPS maps were combined with the LABOCA map to
distinguish between pre- and protostellar cores, and to estimate the
evolutionary stages of protostars. Twelve dense cores were detected at 870
micron in the Ori B9 cloud. The submm cores constitute ~4% of the total mass of
the Ori B9 region. There is an equal number of pre- and protostellar cores. Two
of the submm sources, which we call SMM 3 and SMM 4, are previously unknown
Class 0 candidates. We found a moderate degree of deuteration in N2H+
(0.03-0.04). There is, furthermore, evidence for N2H+ depletion in the core SMM
4. We derive a relatively high degree of ionization (~10^-7) in the clump
associated with IRAS 05405-0117. The ambipolar diffusion timescales for two of
the cores are ~70-100 times longer than the free-fall time. The distribution
and masses of dense cores in Ori B9 are similar to those observed in more
active regions in Orion, where the statistical core properties have been
explained by turbulent fragmentation. The 50/50 proportions of pre- and
protostellar cores suggest that duration of the prestellar phase is comparable
to the free-fall time. This timescale can be questioned, however, on the basis
of chemical data on the IRAS 05405-0117 region. A possible explanation is that
this survey samples only the densest, i.e., dynamically most advanced cores.Comment: 17 pages, 8 figures, 9 tables. Revised version accepted for
publication in A&A (Figs. 1, 3, and 7 revised, minor revisions in Sect. 5.2,
updated reference information
Spectral line survey of the ultracompact HII region Mon R2
Ultracompact (UC) HII regions constitute one of the earliest phases in the
formation of a massive star and are characterized by extreme physical
conditions (Go>10^5 Habing field and n>10^6 cm^-3). The UC HII Mon R2 is the
closest one and therefore an excellent target to study the chemistry in these
complex regions.
We carried out a 3mm and 1mm spectral survey using the IRAM 30-m telescope
towards three positions that represent different physical environments in Mon
R2: (i) the ionization front (IF) at (0",0"); two peaks in the molecular cloud
(ii) MP1 at the offset (+15",-15") and (iii) MP2 at the farther offset
(0",40"). In addition, we carried out extensive modeling to explain the
chemical differences between the three observed regions.
We detected more than thirty different species. We detected SO+ and C4H
suggesting that UV radiation plays an important role in the molecular chemistry
of this region. We detected the typical PDR molecules CN, HCN, HCO, C2H, and
c-C3H2. While the IF and the MP1 have a chemistry similar to that found in high
UV field and dense PDRs like the Orion Bar, the MP2 is more similar to lower
UV/density PDRs like the Horsehead nebula.
We also detected complex molecules that are not usually found in PDRs (CH3CN,
H2CO, HC3N, CH3OH and CH3C2H). Sulfur compounds CS, HCS+, C2S, H2CS, SO and SO2
and the deuterated species DCN and C2D were also identified. [DCN]/[HCN]=0.03
and [C2D]/[C2H]=0.05, are among the highest in warm regions.
Our results show that the high UV/dense PDRs present a different chemistry
from that of the low UV case. Abundance ratios like [CO+]/[HCO+] or
[HCO]/[HCO+] are good diagnostics to differentiate between them. In Mon R2 we
have the two classes of PDRs, a high UV PDR towards the IF and the adjacent
molecular bar and a low-UV PDR which extends towards the north-west following
the border of the cloud.Comment: 31 page
First detection of gas-phase ammonia in a planet-forming disk. NH₃, N₂H⁺, and H₂O in the disk around TW Hydrae
Context. Nitrogen chemistry in protoplanetary disks and the freeze-out on dust particles is key for understanding the formation of nitrogen-bearing species in early solar system analogs. In dense cores, 10% to 20% of the nitrogen reservoir is locked up in ices such as NH3, NH4+ and OCN−. So far, ammonia has not been detected beyond the snowline in protoplanetary disks. Aims. We aim to find gas-phase ammonia in a protoplanetary disk and characterize its abundance with respect to water vapor. Methods. Using HIFI on the Herschel Space Observatory, we detected for the first time the ground-state rotational emission of ortho-NH3 in a protoplanetary disk around TW Hya. We used detailed models of the disk’s physical structure and the chemistry of ammonia and water to infer the amounts of gas-phase molecules of these species. We explored two radial distributions (extended across the disk and confined to <60 au like the millimeter-sized grains) and two vertical distributions (near the midplane and at intermediate heights above the midplane, where water is expected to photodesorb off icy grains) to describe the (unknown) location of the molecules. These distributions capture the effects of radial drift and vertical settling of ice-covered grains. Results. The NH310–00 line is detected simultaneously with H2O 110–101 at an antenna temperature of 15.3 mK in the Herschel beam; the same spectrum also contains the N2H+ 6–5 line with a strength of 18.1 mK. We use physical-chemical models to reproduce the fluxes and assume that water and ammonia are cospatial. We infer ammonia gas-phase masses of 0.7−11.0 × 1021 g, depending on the adopted spatial distribution, in line with previous literature estimates. For water, we infer gas-phase masses of 0.2−16.0 × 1022 g, improving upon earlier literature estimates This corresponds to NH3/H2O abundance ratios of 7%−84%, assuming that water and ammonia are co-located. The inferred N2H+ gas mass of 4.9 × 1021 g agrees well with earlier literature estimates that were based on lower excitation transitions. These masses correspond to a disk-averaged abundances of 0.2−17.0 × 10-11, 0.1−9.0 × 10-10 and 7.6 × 10-11 for NH3, H2O and N2H+ respectively. Conclusions. Only in the most compact and settled adopted configuration is the inferred NH3/H2O consistent with interstellar ices and solar system bodies of ~5%–10%; all other spatial distributions require additional gas-phase NH3 production mechanisms. Volatile release in the midplane may occur through collisions between icy bodies if the available surface for subsequent freeze-out is significantly reduced, for instance, through growth of small grains into pebbles or larger bodies
Health plan administrative records versus birth certificate records: quality of race and ethnicity information in children
<p>Abstract</p> <p>Background</p> <p>To understand racial and ethnic disparities in health care utilization and their potential underlying causes, valid information on race and ethnicity is necessary. However, the validity of pediatric race and ethnicity information in administrative records from large integrated health care systems using electronic medical records is largely unknown.</p> <p>Methods</p> <p>Information on race and ethnicity of 325,810 children born between 1998-2008 was extracted from health plan administrative records and compared to birth certificate records. Positive predictive values (PPV) were calculated for correct classification of race and ethnicity in administrative records compared to birth certificate records.</p> <p>Results</p> <p>Misclassification of ethnicity and race in administrative records occurred in 23.1% and 33.6% children, respectively; the majority due to missing ethnicity (48.3%) and race (40.9%) information. Misclassification was most common in children of minority groups. PPV for White, Black, Asian/Pacific Islander, American Indian/Alaskan Native, multiple and other was 89.3%, 86.6%, 73.8%, 18.2%, 51.8% and 1.2%, respectively. PPV for Hispanic ethnicity was 95.6%. Racial and ethnic information improved with increasing number of medical visits. Subgroup analyses comparing racial classification between non-Hispanics and Hispanics showed White, Black and Asian race was more accurate among non-Hispanics than Hispanics.</p> <p>Conclusions</p> <p>In children, race and ethnicity information from administrative records has significant limitations in accurately identifying small minority groups. These results suggest that the quality of racial information obtained from administrative records may benefit from additional supplementation by birth certificate data.</p
Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling
Prostaglandins, particularly prostaglandin E2 (PGE2), play an important role during inflammation. This is exemplified by the clinical use of cyclooxygenase 2 inhibitors, which interfere with PGE2 synthesis, as effective antiinflammatory drugs. Here, we show that PGE2 directly promotes differentiation and proinflammatory functions of human and murine IL-17–producing T helper (Th17) cells. In human purified naive T cells, PGE2 acts via prostaglandin receptor EP2- and EP4-mediated signaling and cyclic AMP pathways to up-regulate IL-23 and IL-1 receptor expression. Furthermore, PGE2 synergizes with IL-1β and IL-23 to drive retinoic acid receptor–related orphan receptor (ROR)-γt, IL-17, IL-17F, CCL20, and CCR6 expression, which is consistent with the reported Th17 phenotype. While enhancing Th17 cytokine expression mainly through EP2, PGE2 differentially regulates interferon (IFN)-γ production and inhibits production of the antiinflammatory cytokine IL-10 in Th17 cells predominantly through EP4. Furthermore, PGE2 is required for IL-17 production in the presence of antigen-presenting cells. Hence, the combination of inflammatory cytokines and noncytokine immunomodulators, such as PGE2, during differentiation and activation determines the ultimate phenotype of Th17 cells. These findings, together with the altered IL-12/IL-23 balance induced by PGE2 in dendritic cells, further highlight the crucial role of the inflammatory microenvironment in Th17 cell development and regulation
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