Acyl-Imidazoles A Privileged Ester Surrogate for Enantioselective Synthesis

International audienceSince the first report by Evans in asymmetric Friedel‐Crafts reactions, the use of acyl‐imidazoles has blossomed as powerful ester/amide surrogates. The imidazole scaffold indeed displays stability and special activation features allowing both better reactivity and selectivity in traditional ester/amide functionalizations: α‐(enolate chemistry), β‐(conjugate additions), α,β‐(cycloadditions) or γ/δ‐(vinylogous). An overview of the contemporary and growing interest in acyl‐imidazoles in metal‐ and organo‐catalyzed transformations (bio‐hybrid catalytic systems will be fully described in a back‐to‐back Minireview) will be highlighted. Moreover, post‐functionalization expediencies are also going to be discussed in this Minireview

In vivo stem cell tracking using scintigraphy in a canine model of DMD

One of the main challenges in cell therapy for muscle diseases is to efficiently target the muscle. To address this issue and achieve better understanding of in vivo cell fate, we evaluated the relevance of a non-invasive cell tracking method in the Golden Retriever Muscular Dystrophy (GRMD) model, a well-recognised model of Duchenne Muscular Dystrophy (DMD). Mesoangioblasts were directly labelled with 111In-oxine, and injected through one of the femoral arteries. The scintigraphy images obtained provided the first quantitative mapping of the immediate biodistribution of mesoangioblasts in a large animal model of DMD. The results revealed that cells were trapped by the first capillary filters: the injected limb and the lung. During the days following injection, radioactivity was redistributed to the liver. In vitro studies, performed with the same cells prepared for injecting the animal, revealed prominent cell death and 111In release. In vivo, cell death resulted in 111In release into the vasculature that was taken up by the liver, resulting in a non-specific and non-cell-bound radioactive signal. Indirect labelling methods would be an attractive alternative to track cells on the mid- and long-term

The Spitzer Extragalactic Representative Volume Survey (SERVS): The Environments of High-z SDSS Quasi-Stellar-Objects

This paper presents a study of the environments of SDSS Quasi-Stellar-Objects (QSOs) in the Spitzer Extragalactic Representative Volume Survey (SERVS). We concentrate on the high-redshift QSOs as these have not been studied in large numbers with data of this depth before. We use the IRAC 3.6-4.5{\mu}m colour of objects and ancillary r-band data to filter out as much foreground contamination as possible. This technique allows us to find a significant (> 4-{\sigma}) over-density of galaxies around QSOs in a redshift bin centred on z ~ 2.0 and a (> 2-{\sigma}) over-density of galaxies around QSOs in a redshift bin centred on z ~ 3.3. We compare our findings to the predictions of a semi-analytic galaxy formation model, based on the {\Lambda}CDM millennium simulation, and find for both redshift bins that the model predictions match well the source-density we have measured from the SERVS data.Comment: 13 pages, 12 figures, Accepted by Ap

NEOWISE Observations of Near-Earth Objects: Preliminary Results

With the NEOWISE portion of the \emph{Wide-field Infrared Survey Explorer} (WISE) project, we have carried out a highly uniform survey of the near-Earth object (NEO) population at thermal infrared wavelengths ranging from 3 to 22 $\mu$m, allowing us to refine estimates of their numbers, sizes, and albedos. The NEOWISE survey detected NEOs the same way whether they were previously known or not, subject to the availability of ground-based follow-up observations, resulting in the discovery of more than 130 new NEOs. The survey's uniformity in sensitivity, observing cadence, and image quality have permitted extrapolation of the 428 near-Earth asteroids (NEAs) detected by NEOWISE during the fully cryogenic portion of the WISE mission to the larger population. We find that there are 981$\pm$19 NEAs larger than 1 km and 20,500$\pm$3000 NEAs larger than 100 m. We show that the Spaceguard goal of detecting 90% of all 1 km NEAs has been met, and that the cumulative size distribution is best represented by a broken power law with a slope of 1.32$\pm$0.14 below 1.5 km. This power law slope produces $\sim13,200\pm$1,900 NEAs with $D>$140 m. Although previous studies predict another break in the cumulative size distribution below $D\sim$50-100 m, resulting in an increase in the number of NEOs in this size range and smaller, we did not detect enough objects to comment on this increase. The overall number for the NEA population between 100-1000 m are lower than previous estimates. The numbers of near-Earth comets will be the subject of future work.Comment: Accepted to Ap

The Properties of the Heterogeneous Shakhbazyan Groups of Galaxies in the SDSS

We present a systematic study of the sub-sample of Shakhbazyan groups (SHKs) covered by the Sloan Digital Sky Survey Data Release--5 (SDSS-5). SHKs probe an environment with characteristics which are intermediate between those of loose and very compact groups. Surprisingly, we found that several groups identifying algorithms (e.g. Berlind et al. 2006, Tago et al. 2008) miss this type of structures. Using the SDSS-5 spectroscopic data and the photometric redshifts derived in D'Abrusco et al. 2007, we identified possible group members in photometric redshift space and derived, for each group, several individual properties. We also combined pointed and stacked Rosat All Sky Survey data to investigate the X-ray luminosities of these systems. Our study confirms that the majority of groups are physical entities with richness in the range 3--13 galaxies, and properties ranging between those of loose and compact groups. We confirm that SHK groups are richer in early-type galaxies than the surrounding environment and the field, as expected from the morphology-density relation and from the selection of groups of red galaxies. Furthermore, our work supports the existence of two sub-classes of structures, the first one being formed by compact and isolated groups and the second formed by extended structures. We suggest that while the first class of objects dwells in less dense regions like the outer parts of clusters or the field, possibly sharing the properties of Hickson Compact Groups, the more extended structures represent a mixture of [core+halo] configurations and cores of rich clusters. X-ray luminosities for SHKs are generally consistent with these results and with the expectations for the L_X-sigma_v relation, but also suggest the velocity dispersions reported in literature are underestimated for some of the richest systems.Comment: 20 pages, 14 figures, 4 tables. Accepted for publication by MNRA

Multiwavelength characterization of faint ultra steep spectrum radio sources: a search for high-redshift radio galaxies

Context. Ultra steep spectrum (USS) radio sources are one of the efficient tracers of powerful high-z radio galaxies (HzRGs). In contrast to searches for powerful HzRGs from radio surveys of moderate depths, fainter USS samples derived from deeper radio surveys can be useful in finding HzRGs at even higher redshifts and in unveiling a population of obscured weaker radio-loud AGN at moderate redshifts. Aims. Using our 325 MHz GMRT observations (5σ ∼ 800 μJy) and 1.4 GHz VLA observations (5σ ∼ 80−100 μJy) available in two subfields (VLA-VIMOS VLT Deep Survey (VLA-VVDS) and Subaru X-ray Deep Field (SXDF)) of the XMM-LSS field, we derive a large sample of 160 faint USS radio sources and characterize their nature. Methods. The optical and IR counterparts of our USS sample sources are searched using existing deep surveys, at respective wavelengths. We attempt to unveil the nature of our faint USS sources using diagnostic techniques based on mid-IR colors, flux ratios of radio to mid-IR, and radio luminosities. Results. Redshift estimates are available for 86/116 (∼74%) USS sources in the VLA-VVDS field and for 39/44 (∼87%) USS sources in the SXDF fields with median values (zmedian) ∼1.18 and ∼1.57, respectively, which are higher than estimates for non-USS radio sources (zmedian non−USS ∼ 0.99 and ∼0.96), in the two subfields. The MIR color–color diagnostic and radio luminosities are consistent with most of our USS sample sources at higher redshifts (z > 0.5) being AGN. The flux ratio of radio to mid-IR (S 1.4 GHz/S 3.6 μm) versus redshift diagnostic plot suggests that more than half of our USS sample sources distributed over z ∼ 0.5 to 3.8 are likely to be hosted in obscured environments. A significant fraction (∼26% in the VLA-VVDS and ∼13% in the SXDF) of our USS sources without redshift estimates mostly remain unidentified in the existing optical, IR surveys, and exhibit high radio to mid-IR flux ratio limits similar to HzRGs, and so, can be considered as potential HzRG candidates. Conclusions. Our study shows that the criterion of ultra steep spectral index remains a reasonably efficient method to select high-z sources even at sub-mJy flux densities. In addition to powerful HzRG candidates, our faint USS sample also contains populations of weaker radio-loud AGNs potentially hosted in obscured environments

Somatic mutations of KIT in familial testicular germ cell tumours

Somatic mutations of the KIT gene have been reported in mast cell diseases and gastrointestinal stromal tumours. Recently, they have also been found in mediastinal and testicular germ cell tumours (TGCTs), particularly in cases with bilateral disease. We screened the KIT coding sequence (except exon 1) for germline mutations in 240 pedigrees with two or more cases of TGCT. No germline mutations were found. Exons 10, 11 and 17 of KIT were examined for somatic mutations in 123 TGCT from 93 multiple-case testicular cancer families. Five somatic mutations were identified; four were missense amino acid substitutions in exon 17 and one was a 12bp in-frame deletion in exon 11. Two of seven TGCT from cases with bilateral disease carried KIT mutations compared with 3 out 116 unilateral cases (p = 0.026). The results indicate that somatic KIT mutations are implicated in the development of a minority of familial as well as sporadic TGCT. They also lend support to the hypothesis that KIT mutations primarily take place during embryogenesis such that primordial germ cells with KIT mutations are distributed to both testes

Inhibitory Effect of TNF-α on Malaria Pre-Erythrocytic Stage Development: Influence of Host Hepatocyte/Parasite Combinations

BACKGROUND: The liver stages of malaria parasites are inhibited by cytokines such as interferon-gamma or Interleukin (IL)-6. Binding of these cytokines to their receptors at the surface of the infected hepatocytes leads to the production of nitric oxide (NO) and radical oxygen intermediates (ROI), which kill hepatic parasites. However, conflicting results were obtained with TNF-alpha possibly because of differences in the models used. We have reassessed the role of TNF-alpha in the different cellular systems used to study the Plasmodium pre-erythrocytic stages. METHODS AND FINDINGS: Human or mouse TNF-alpha were tested against human and rodent malaria parasites grown in vitro in human or rodent primary hepatocytes, or in hepatoma cell lines. Our data demonstrated that TNF-alpha treatment prevents the development of malaria pre-erythrocytic stages. This inhibitory effect however varies with the infecting parasite species and with the nature and origin of the cytokine and hepatocytes. Inhibition was only observed for all parasite species tested when hepatocytes were pre-incubated 24 or 48 hrs before infection and activity was directed only against early hepatic parasite. We further showed that TNF-alpha inhibition was mediated by a soluble factor present in the supernatant of TNF-alpha stimulated hepatocytes but it was not related to NO or ROI. Treatment TNF-alpha prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. CONCLUSIONS: Treatment TNF-alpha prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. However, the nature of the cytokine-host cell-parasite combination must be carefully considered for extrapolation to the human infection