Protein refolding in an oscillatory flow reactor

We demonstrate that an oscillatory flow reactor is a viable reactor for protein refolding via direct dilution. The mixing characteristics of the oscillatory flow reactor are well described and controllable and, importantly, can be scaled-up to process scale without a loss of mixing efficiency. This makes the oscillatory flow reactor an attractive alternative to conventional stirred-tank reactors for process-scale renaturation

Gas Around Active Galactic Nuclei and New Phase Calibration Strategies for High-Frequency VLBI

We have a project to measure magnetic fields in the obscuring tori of AGNs. We observed five free-free absorbed jets in radio galaxies with polarimetric VLBI at 15 GHz to determine Faraday rotation measures. Surprisingly, all sources are unpolarized, putting severe constraints on the degree of magnetic field turbulence and the gas distribution in these objects. We have further carried out multi-frequency VLBI monitoring of the Seyfert 2 galaxy NGC 3079 and find unusually steep and inverted spectra in the radio components, and derive general properties of Seyfert galaxies using published VLBI observations of Seyfert galaxies. We have developed a new phase-referencing technique for high frequency VLBI observations with the VLBA. Instead of inserting short scans on a calibrator into the target source observations, the target source is continuously observed while rapidly switching between the target frequency and a lower reference frequency. We demonstrate that the technique allows phase calibration almost reaching the thermal noise limit and present the first detection of the AGN in NGC 4261 at 86 GHz. This is the weakest source ever detected with VLBI at that frequency.Comment: PhD thesis, 188 page

VLBI detection of an Infrared-Faint Radio Source

Infrared-Faint Radio Sources represent a new and unexpected class of object which is bright at radio wavelengths but unusually faint at infrared wavelengths. If, like most mJy radio sources, they were either conventional active or star-forming galaxies in the local Universe, we would expect them to be detectable at infrared wavelengths, and so their non-detection by the Spitzer Space Telescope is surprising. Here we report the detection of one of these sources using Very Long Baseline Interferometry, from which we conclude that the sources are driven by Active Galactic Nuclei. We suggest that these sources are either normal radio-loud quasars at high redshift or abnormally obscured radio galaxies.Comment: accepted by MNRA

Automated Editing of Radio Interferometer Data with Pieflag

Editing of radio interferometer data, a process commonly known as ``flagging'', can be laborious and time-consuming. One quickly tends to flag more data than actually required, sacrificing sensitivity and image fidelity in the process. I describe a program, Pieflag, which can analyse radio interferometer data to filter out measurements which are likely to be affected by interference. Pieflag uses two algorithms to allow for data sets which are either dominated by receiver noise or by source structure. Together, the algorithms detect essentially all affected data whilst the amount of data which is not affected by interference but falsely marked as such is kept to a minimum. The sections marked by Pieflag are very similar to what would be deemed affected by the observer in a visual inspection of the data. Pieflag displays its results concisely and allows the user to add and remove flags interactively. It is written in Python, is easy to install and use, and has a variety of options to adjust its algorithms to a particular observing situation. I describe how Pieflag works and illustrate its effect using data from typical observations.Comment: 6 pages, 5 figures, requires apj.bst, accepted for publication in Publications of the Astronomical Society of Australi

Unveiling the origin of the radio emission in radio-quiet quasars

The origin of the radio emission in radio-quiet quasars (RQQs) has been a matter of debate for a long time. It is not well understood whether the emission is caused by star formation in the host galaxy or by black hole activity of the active galactic nuclei (AGN). We shed some light on these questions using the Very Long Baseline Interferometry (VLBI) technique to search for RQQs in the field of the Cosmological Evolution Survey (COSMOS). The extensive multi-wavelength coverage of the field (from radio to X-rays) was used to classify RQQs, and the milli-arcsecond resolution of VLBI provides a direct way to identify AGNs. In a sample of 18 RQQs we detected 3 using the Very Long Baseline Array (VLBA) at 1.4 GHz. In this letter we report for the first time on a sample of RQQs with a measured lower limit on the fraction of radio emission coming from the AGN, thus demonstrating that the radio emission of at least some RQQs is dominated by an AGN.Comment: 4 pages, letter to the editor, accepted for publication in A&

Infrared-Faint Radio Sources: A Cosmological View - AGN Number Counts, the Cosmic X-Ray Background and SMBH Formation

Context. Infrared Faint Radio Sources (IFRS) are extragalactic emitters clearly detected at radio wavelengths but barely detected or undetected at optical and infrared wavelengths, with 5 sigma sensitivities as low as 1 uJy. Aims. Recent SED-modelling and analysis of their radio properties shows that IFRS are consistent with a population of (potentially extremely obscured) high-redshift AGN at 3<z<6. We demonstrate some astrophysical implications of this population and compare them to predictions from models of galaxy evolution and structure formation. Methods. We compiled a list of IFRS from four deep extragalactic surveys and extrapolated the IFRS number density to a survey-independent value of (30.8 +- 15.0) per square degree. We computed the IFRS contribution to the total number of AGN in the Universe to account for the Cosmic X-ray Background. By estimating the black hole mass contained in IFRS, we present conclusions for the SMBH mass density in the early universe and compare it to relevant simulations of structure formation after the Big Bang. Results. The number density of AGN derived from the IFRS density was found to be about 310 deg^-2, which is equivalent to a SMBH mass density of the order of 10^3 M_sun Mpc^-3 in the redshift range 3<z<6. This produces an X-ray flux of 9 10^-16 W m^-2 deg^-2 in the 0.5-2.0 keV band and 3 10^-15 W m^-2 deg^-2 in the 2.0-10 keV band, in agreement with the missing unresolved components of the Cosmic X-ray Background. Concerning the problem of SMBH formation after the Big Bang we find evidence for a scenario involving both halo gas accretion and major mergers.Comment: 8 pages, 4 figures, accepted for publication in A&