"Employment in Poland 2011 - Poverty and jobs"

This edition is devoted to the issue of poverty. Growth of prosperity is not distributed evenly among the Polish citizens, and some of them still cannot satisfy even basic needs. The improvement of an overall economic situation reduces risk of poverty in general. However, in case the risk occurs, efficient and effective support measures should be provided. First part of the report defines poverty, and explains the extent of the connection with such phenomena as economic deprivation or social exclusion. It is vital, as the exact criterion of poverty is ambiguous. Having discussed these issues, we elaborate on the characteristics of poverty in Poland as compared to those observed in other Member States of the European Union, as well as their regional and local dimensions. The key element of this part is to determine the causes of poverty, i.e. to what extent poverty results from the features of national and regional economy, and to what extent from individual characteristics of people. The topics introduced in this part form background for subsequent parts of the report. The second part is devoted to the poverty of certain social groups such as elderly, children, unemployed and rural. Conducted analyses allow to dispel some of the myths in this matter, as well as to bring out the characteristics that are particularly important from the perspective of creating the support instruments for groups that are at risk of poverty. The issue of poverty among children is scrutinised from the intergenerational perspective, and its determinants and possible measures of preventing the phenomenon are discussed. The third part focuses on relations between income inequalities and situation on the labour market. In this part changes in distribution of wages in Poland are confronted with the poverty dynamics. They are supplemented with a reflection on the impact on poverty exerted by changes in the Polish society, including (but not limited to) those concerning household structure or economic activity of women. The phenomenon of so called working poor is also scrutinised here. We determine, inter alia, whether the characteristics of household or of work performed are more important causes of in-work poverty. We also elaborate on the question if situation of poor households is persistent or low-paid employment enables them to get out of poverty. The fourth part discusses available tools and instruments to fight the poverty. The state of affairs described in first three parts allows to take a closer look on efficiency and effectiveness of social policy measures applied in Poland, both tax and benefit based. Special attention is drawn to the issue of financial support of families with children as an element of social and demographic policy. The key features of this part are simulations of the impact of hypothetical changes in currently functioning system on rate of poverty in Poland. The recommendations for public policy constitute the summary of the report

Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells

The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells within 3–4 weeks by using Gateway® cloning to build the target vectors. The cDNA of the gene of interest can be expressed either directly by the ROSA26 promoter providing a moderate level of expression or by a CAGG promoter placed in the ROSA26 locus providing higher transgene expression. Utilization of F1 hybrid ES cells with exceptional developmental potential allows the production of germ line transmitting, fully or highly ES cell-derived mice by aggregation of cells with diploid embryos. The presented streamlined procedures accelerate the examination of phenotypical consequences of transgene expression. It also provides a unique tool for comparing the biological activity of polymorphic or splice variants of a gene, or products of different genes functioning in the same or parallel pathways in an overlapping manner

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts