Deliberation and Identity Rules : The Effect of Anonymity, Pseudonyms and Real-Name Requirements on the Cognitive Complexity of Online News Comments

How do identity rules influence online deliberation? We address this question by drawing on a data set of 45 million comments on news articles on the Huffington Post from January 2013 to May 2015. At the beginning of this period, the site allowed commenting under what we call non-durable pseudonyms. In December 2013, Huffington Post moved to regulate its forum by requiring users to authenticate their accounts. And in June 2014, Huffington Post outsourced commenting to Facebook altogether, approximating a ‘real-name’ environment. We find a significant increase in the cognitive complexity of comments (a proxy for one aspect of deliberative quality) during the middle phase, followed by a decrease following the shift to real-name commenting through Facebook. Our findings challenge the terms of the apparently simple trade-off between the goods and bads of anonymous and real-name environments and point to the potential value of durable pseudonymity in the context of online discussion

Reprogramming the Mycobacterium tuberculosis transcriptome during pathogenesis

Transcriptional profiling has revealed that Mycobacterium tuberculosis adapts both its metabolic and respiratory states during infection, utilising lipids as a carbon source and switching to alternative electron acceptors. These global gene expression datasets may be exploited to identify virulence determinants and to screen for new targets for rational drug design. Characterising the changing physiological predicament of distinct M. tb populations during infection will help expose the fundamental biology of M. tb highlighting mechanisms that influence tuberculosis pathogenicity

Ab Initio Screening Approach for the Discovery of Lignin Polymer Breaking Pathways

The directed depolymerization of lignin biopolymers is of utmost relevance for the valorization or commercialization of biomass fuels. We present a computational and theoretical screening approach to identify potential cleavage pathways and resulting fragments that are formed during depolymerization of lignin oligomers containing two to six monomers. We have developed a chemical discovery technique to identify the chemically relevant putative fragments in eight known polymeric linkage types of lignin. Obtaining these structures is a crucial precursor to the development of any further kinetic modeling. We have developed this approach by adapting steered molecular dynamics calculations under constant force and varying the points of applied force in the molecule to diversify the screening approach. Key observations include relationships between abundance and breaking frequency, the relative diversity of potential pathways for a given linkage, and the observation that readily cleaved bonds can destabilize adjacent bonds, causing subsequent automatic cleavage.Massachusetts Institute of Technology (Research Support Corporation, Reed Grant)United States. Dept. of Energy. Computational Science Graduate Fellowship Program (DOE-CSGF)Burroughs Wellcome Fund (Career Award at the Scientific Interface

Cytological and transcript analyses reveal fat and lazy persister-like bacilli in tuberculous sputum

As nonreplicating tubercle bacilli are tolerant to the cidal action of antibiotics and resistant to multiple stresses, identification of this persister-like population of tubercle bacilli in sputum presents exciting and tractable new opportunities to investigate both responses to chemotherapy and the transmission of tuberculosis

A Human-Computer Duet System for Music Performance

Virtual musicians have become a remarkable phenomenon in the contemporary multimedia arts. However, most of the virtual musicians nowadays have not been endowed with abilities to create their own behaviors, or to perform music with human musicians. In this paper, we firstly create a virtual violinist, who can collaborate with a human pianist to perform chamber music automatically without any intervention. The system incorporates the techniques from various fields, including real-time music tracking, pose estimation, and body movement generation. In our system, the virtual musician's behavior is generated based on the given music audio alone, and such a system results in a low-cost, efficient and scalable way to produce human and virtual musicians' co-performance. The proposed system has been validated in public concerts. Objective quality assessment approaches and possible ways to systematically improve the system are also discussed

Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages

This study provides the proof of principle that probing the host and the microbe transcriptomes simultaneously is a valuable means to accessing unique information on host pathogen interactions. Our results also underline the extraordinary plasticity of host cell and pathogen responses to infection, and provide a solid framework to further understand the complex mechanisms involved in immunity to M. tuberculosis and in mycobacterial adaptation to different intracellular environments

Understanding anti-tuberculosis drug efficacy: rethinking bacterial populations and how we model them

Tuberculosis still remains a global health emergency, claiming 1.5 million lives in 2013. The bacterium responsible for this disease, Mycobacterium tuberculosis (M.tb), has successfully survived within hostile host environments, adapting to immune defence mechanisms, for centuries. This has resulted in a disease that is challenging to treat, requiring lengthy chemotherapy with multi-drug regimens. One explanation for this difficulty in eliminating M.tb bacilli in vivo is the disparate action of antimicrobials on heterogeneous populations of M.tb, where mycobacterial physiological state may influence drug efficacy. In order to develop improved drug combinations that effectively target diverse mycobacterial phenotypes, it is important to understand how such subpopulations of M.tb are formed during human infection. We review here the in vitro and in vivo systems used to model M.tb subpopulations that may persist during drug therapy, and offer aspirations for future research in this field

Contemporary Trends Of Deliberative Research: Synthesizing A New Study Agenda

Deliberation is among the most widely acknowledged figures of thought in social theory. Taking the growing interest in the research conducted around deliberative democracy as an initial position, this paper seeks to provide an overview of recent predispositions and paradigm shifts of approaches taken towards the analysis of real-world discourses. Therefore, as a first step three different – nevertheless correlating – trends of deliberative research are identified: (1) an “empirical turn” and an effort to test and “falsify” assumptions of deliberative theories, (2) the consideration of certain epistemic dimensions of deliberative democracy and (3) the conceptual opening towards not fully rationalizable modes of communication. Based on those trends, the task is to make suggestions of how to incorporate those predispositions and paradigm shifts fruitfully into future research designs