48 research outputs found
The species-specific regenerative effects of notochordal cell-conditioned medium on chondrocyte-like cells derived from degenerated human intervertebral discs
During intervertebral disc (IVD) maturation, the main cell type shifts from notochordal cells (NCs) to chondrocytelike cells (CLCs). NCs secrete factors with regenerative potential, making them an interesting focus for regenerative treatments. During initial development, these strategies preferably employ non-human donors due to easy availability of their NC-rich nucleus pulposus (NP) tissue. To increase the success of translating these strategies for clinical application, this study aimed to delineate whether NC-secreted factors of different species have a regenerative effect on human CLCs. Human, canine and porcine NC-rich NP tissue and NC-conditioned medium (NCCM) were analysed biochemically and histologically. Human CLC micro-aggregates from degenerated IVDs were cultured in human, canine or porcine NCCM. Collagen, glycosaminoglycan (GAG) and DNA content was determined and histology was performed. Canine and porcine NPs were richer in NCs than human NPs. Human NPs contained the highest collagen content, whereas the DNA and GAG content of canine NPs was significantly higher than that of human or porcine NPs. NCCM from all species significantly increased the DNA and GAG content of the human CLC micro-aggregates. Porcine and canine NCCM were significantly more potent than human NCCM in inducing GAG deposition, whereas only human NCCM induced collagen type II production. Secreted factors from human, canine and porcine NC-rich NPs exerted regenerative effects on human CLCs, indicating a cross-species effect. Bioactive compound(s) are present in NCCM of different species that may reverse human IVD degeneration, supporting further research into strategies based on NC-technology employing canine or porcine models for their translation into humans.</p
Mean field approach to antiferromagnetic domains in the doped Hubbard model
We present a restricted path integral approach to the 2D and 3D repulsive
Hubbard model. In this approach the partition function is approximated by
restricting the summation over all states to a (small) subclass which is chosen
such as to well represent the important states. This procedure generalizes mean
field theory and can be systematically improved by including more states or
fluctuations. We analyze in detail the simplest of these approximations which
corresponds to summing over states with local antiferromagnetic (AF) order. If
in the states considered the AF order changes sufficiently little in space and
time, the path integral becomes a finite dimensional integral for which the
saddle point evaluation is exact. This leads to generalized mean field
equations allowing for the possibility of more than one relevant saddle points.
In a big parameter regime (both in temperature and filling), we find that this
integral has {\em two} relevant saddle points, one corresponding to finite AF
order and the other without. These degenerate saddle points describe a phase of
AF ordered fermions coexisting with free, metallic fermions. We argue that this
mixed phase is a simple mean field description of a variety of possible
inhomogeneous states, appropriate on length scales where these states appear
homogeneous. We sketch systematic refinements of this approximation which can
give more detailed descriptions of the system.Comment: 14 pages RevTex, 6 postscript figures included using eps
Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in âs = 7 TeV pp collisions with the ATLAS detector
A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fbâ1 of protonâproton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results
Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC
Measurements of inclusive jet suppression in heavy ion collisions at the LHC
provide direct sensitivity to the physics of jet quenching. In a sample of
lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated
luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with
a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the
transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the
anti-kt algorithm with values for the distance parameter that determines the
nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of
the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp.
Jet production is found to be suppressed by approximately a factor of two in
the 10% most central collisions relative to peripheral collisions. Rcp varies
smoothly with centrality as characterized by the number of participating
nucleons. The observed suppression is only weakly dependent on jet radius and
transverse momentum. These results provide the first direct measurement of
inclusive jet suppression in heavy ion collisions and complement previous
measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables,
submitted to Physics Letters B. All figures including auxiliary figures are
available at
http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02
A Kinematical Approach to Conformal Cosmology
We present an alternative cosmology based on conformal gravity, as originally
introduced by H. Weyl and recently revisited by P. Mannheim and D. Kazanas.
Unlike past similar attempts our approach is a purely kinematical application
of the conformal symmetry to the Universe, through a critical reanalysis of
fundamental astrophysical observations, such as the cosmological redshift and
others. As a result of this novel approach we obtain a closed-form expression
for the cosmic scale factor R(t) and a revised interpretation of the space-time
coordinates usually employed in cosmology. New fundamental cosmological
parameters are introduced and evaluated. This emerging new cosmology does not
seem to possess any of the controversial features of the current standard
model, such as the presence of dark matter, dark energy or of a cosmological
constant, the existence of the horizon problem or of an inflationary phase.
Comparing our results with current conformal cosmologies in the literature, we
note that our kinematic cosmology is equivalent to conformal gravity with a
cosmological constant at late (or early) cosmological times. The cosmic scale
factor and the evolution of the Universe are described in terms of several
dimensionless quantities, among which a new cosmological variable delta emerges
as a natural cosmic time. The mathematical connections between all these
quantities are described in details and a relationship is established with the
original kinematic cosmology by L. Infeld and A. Schild. The mathematical
foundations of our kinematical conformal cosmology will need to be checked
against current astrophysical experimental data, before this new model can
become a viable alternative to the standard theory.Comment: Improved version, with minor changes. 58 pages, including 7 figures
and one table. Accepted for publication in General Relativity and Gravitation
(GERG
Global data on earthworm abundance, biomass, diversity and corresponding environmental properties
14 p.Earthworms are an important soil taxon as ecosystem engineers, providing a variety of crucial ecosystem functions and services. Little is known about their diversity and distribution at large spatial scales, despite the availability of considerable amounts of local-scale data. Earthworm diversity data, obtained from the primary literature or provided directly by authors, were collated with information on site locations, including coordinates, habitat cover, and soil properties. Datasets were required, at a minimum, to include abundance or biomass of earthworms at a site. Where possible, site-level species lists were included, as well as the abundance and biomass of individual species and ecological groups. This global dataset contains 10,840 sites, with 184 species, from 60 countries and all continents except Antarctica. The data were obtained from 182 published articles, published between 1973 and 2017, and 17 unpublished datasets. Amalgamating data into a single global database will assist researchers in investigating and answering a wide variety of pressing questions, for example, jointly assessing aboveground and belowground biodiversity distributions and drivers of biodiversity change
Harmonization and standardization of nucleus pulposus cell extraction and culture methods
Background
In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources.
Methods
The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated.
Results
Recommended protocols are provided for extraction, expansion, and re-differentiation of NP cells from common species utilized for NP cell culture.
Conclusions
This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60â100âU/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide
Long-range Angular Correlations On The Near And Away Side In P-pb Collisions At âsnn=5.02 Tev
7191/Mar294