Lectin-gated and glycan functionalized mesoporous silica nanocontainers for targeting cancer cells overexpressing Lewis X antigen

[EN] Gated mesoporous silica nanoparticles can deliver payload upon the application of a predefined stimulus, and therefore are promising drug delivery systems. Despite their important role, relatively low emphasis has been placed on the design of gating systems that actively target carbohydrate tumor cell membrane receptors. We describe herein a new Lewis X (Le(x)) antigen-targeted delivery system comprising mesoporous silica nanoparticles (MSNs) loaded with ATTO 430LS dye, functionalized with a Le(x) derivative (1) and capped with a fucose-specific carbohydrate-binding protein (Aleuria aurantia lectin (AAL)). This design takes advantage of the affinity of AAL for Le(x) overexpressed receptors in certain cancer cells. In the proximity of the cells, AAL is detached from MSNs to bind Le(x), and selectins in the cells bind Le(x) in the gated MSNs, thereby inducing cargo delivery. Gated MSNs are nontoxic to colon cancer DLD-1 cells, and ATTO 430LS dye delivered correlated with the amount of Le(x) antigen overexpressed at the DLD-1 cell surface. This is one of the few examples of MSNs using biologically relevant glycans for both capping (via interaction with AAL) and targeting (via interaction with overexpressed Le(x) at the cell membrane).The authors thank the Spanish Government (Projects MAT2015-64139-C4-1-R and MAT2013-46101-R (MINECO/ FEDER)), Fondo de Investigacion Sanitaria (PI15/00480) and Generalitat Valenciana (Project PROMETEOII/2014/047 and project GVA/2014/13) for support. R. B. is thankful to Svagata. Eu (Erasmus Mundus Action-II program) for his fellowship. The authors also thank the Electron Microscopy Service at the UPV for support.Bhat, R.; García, I.; Aznar, E.; Arnáiz, B.; Martínez-Bisbal, M.; Liz-Marzán, L.; Penadés, S.... (2018). 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Metallicity of solar-type stars with debris discs and planets

Around 16% of the solar-like stars in our neighbourhood show IR-excesses due to debris discs and a fraction of them are known to host planets. We aim to determine in a homogeneous way the metallicity of a sample of stars with known debris discs and planets. Our analysis includes the calculation of the fundamental stellar parameters by applying the iron ionisation equilibrium conditions to several isolated Fe I and Fe II lines. The metallicity distributions of the different stellar samples suggest that there is a transition toward higher metallicities from stars with neither debris discs nor planets to stars hosting giant planets. Stars with debris discs and stars with neither debris nor planets follow a similar metallicity distribution, although the distribution of the first ones might be shifted towards higher metallicities. Stars with debris discs and planets have the same metallicity behaviour as stars hosting planets, irrespective of whether the planets are low-mass or gas giants. In the case of debris discs and giant planets, the planets are usually cool, -semimajor axis larger than 0.1 AU. The data also suggest that stars with debris discs and cool giant planets tend to have a low dust luminosity, and are among the less luminous debris discs known. We also find evidence of an anticorrelation between the luminosity of the dust and the planet eccentricity. Our data show that the presence of planets, not the debris disc, correlates with the stellar metallicity. The results confirm that core-accretion models represent suitable scenarios for debris disc and planet formation. Dynamical instabilities produced by eccentric giant planets could explain the suggested dust luminosity trends observed for stars with debris discs and planets.Comment: Accepted for publication by A&A, 17 pages, 10 figure

A spectroscopy study of nearby late-type stars, possible members of stellar kinematic groups

Nearby late-type stars are excellent targets for seeking young objects in stellar associations and moving groups. The origin of these structures is still misunderstood, and lists of moving group members often change with time and also from author to author. Most members of these groups have been identified by means of kinematic criteria, leading to an important contamination of previous lists by old field stars. We attempt to identify unambiguous moving group members among a sample of nearby-late type stars by studying their kinematics, lithium abundance, chromospheric activity, and other age-related properties. High-resolution echelle spectra ($R \sim 57000$) of a sample of nearby late-type stars are used to derive accurate radial velocities that are combined with the precise Hipparcos parallaxes and proper motions to compute galactic-spatial velocity components. Stars are classified as possible members of the classical moving groups according to their kinematics. The spectra are also used to study several age-related properties for young late-type stars, i.e., the equivalent width of the lithium Li~{\sc i} \space 6707.8 \space \AA \space line or the $R'_{\rm HK}$ index. Additional information like X-ray fluxes from the ROSAT All-Sky Survey or the presence of debris discs is also taken into account. The different age estimators are compared and the moving group membership of the kinematically selected candidates are discussed. From a total list of 405 nearby stars, 102 have been classified as moving group candidates according to their kinematics. i.e., only $\sim$ 25.2 \% of the sample. The number reduces when age estimates are considered, and only 26 moving group candidates (25.5\% of the 102 candidates) have ages in agreement with the star having the same age as an MG memberComment: 39 pages, 11 figures. Accepted for publication in Astronomy \& Astrophysic

Chromospheric activity and rotation of FGK stars in the solar vicinity. An estimation of the radial velocity jitter

Context: Chromospheric activity produces both photometric and spectroscopic variations that can be mistaken as planets. Large spots crossing the stellar disc can produce planet-like periodic variations in the light curve of a star. These spots clearly affect the spectral line profiles and their perturbations alter the line centroids creating a radial velocity jitter that might contaminate" the variations induced by a planet. Precise chromospheric activity measurements are needed to estimate the activity-induced noise that should be expected for a given star. Aims: We obtain precise chromospheric activity measurements and projected rotational velocities for nearby (d < 25 pc) cool (spectral types F to K) stars, to estimate their expected activity-related jitter. As a complementary objective, we attempt to obtain relationships between fluxes in different activity indicator lines, that permit a transformation of traditional activity indicators, i.e, CaII H & K lines, to others that hold noteworthy advantages. Methods: We used high resolution (~50000) echelle optical spectra. To determine the chromospheric emission of the stars in the sample, we used the spectral subtraction technique. Rotational velocities were determined using the cross-correlation technique. To infer activity-related radial velocity (RV) jitter, we used empirical relationships between this jitter and the R'_HK index. Results: We measured chromospheric activity, as given by different indicators throughout the optical spectra, and projected rotational velocities for 371 nearby cool stars. We have built empirical relationships among the most important chromospheric emission lines. Finally, we used the measured chromospheric activity to estimate the expected RV jitter for the active stars in the sample.Comment: Accepted for publication in Astronomy & Astrophysic

A peculiar class of debris disks from Herschel/DUNES - A steep fall off in the far infrared

Aims. We present photometric data of debris disks around HIP 103389 (HD 199260), HIP 107350 (HN Peg, HD206860), and HIP 114948 (HD 219482), obtained in the context of our Herschel Open Time Key Program DUNES (DUst around NEarby Stars). Methods. We used Herschel/PACS to detect the thermal emission of the three debris disks with a 3 sigma sensitivity of a few mJy at 100 um and 160 um. In addition, we obtained Herschel/PACS photometric data at 70 um for HIP 103389. Two different approaches are applied to reduce the Herschel data to investigate the impact of data reduction on the photometry. We fit analytical models to the available spectral energy distribution (SED) data. Results. The SEDs of the three disks potentially exhibit an unusually steep decrease at wavelengths > 70 um. We investigate the significance of the peculiar shape of these SEDs and the impact on models of the disks provided it is real. Our modeling reveals that such a steep decrease of the SEDs in the long wavelength regime is inconsistent with a power-law exponent of the grain size distribution -3.5 expected from a standard equilibrium collisional cascade. In contrast, a very distinct range of grain sizes is implied to dominate the thermal emission of such disks. However, we demonstrate that the understanding of the data of faint sources obtained with Herschel is still incomplete and that the significance of our results depends on the version of the data reduction pipeline used. Conclusions. A new mechanism to produce the dust in the presented debris disks, deviations from the conditions required for a standard equilibrium collisional cascade (grain size exponent of -3.5), and/or significantly different dust properties would be necessary to explain the potentially steep SED shape of the three debris disks presented. (abridged)Comment: 14 pages, 4 figures, accepted by A&

The unusual protoplanetary disk around the T Tauri star ET Cha

We present new continuum and line observations, along with modelling, of the faint (6-8) Myr old T Tauri star ET Cha belonging to the eta Chamaeleontis cluster. We have acquired HERSCHEL/PACS photometric fluxes at 70 mic and 160 mic, as well as a detection of the [OI] 63 mic fine-structure line in emission, and derived upper limits for some other far-IR OI, CII, CO and o-H2O lines. The HERSCHEL data is complemented by new ANDICAM B-K photometry, new HST/COS and HST/STIS UV-observations, a non-detection of CO J=3-2 with APEX, re-analysis of a UCLES high-resolution optical spectrum showing forbidden emission lines like [OI] 6300A, [SII] 6731A and 6716A, and [NII] 6583A, and a compilation of existing broad-band photometric data. We used the thermo-chemical disk code ProDiMo and the Monte-Carlo radiative transfer code MCFOST to model the protoplanetary disk around ET Cha. Based on these models we can determine the disk dust mass Mdust = (2.E-8 - 5.E-8) Msun, whereas the total disk gas mass is found to be only little constrained, Mgas = (5.E-5 - 3.E-3) Msun. In the models, the disk extends from 0.022 AU (just outside of the co-rotation radius) to only about 10 AU. Larger disks are found to be inconsistent with the CO J=3-2 non-detection. The low velocity component of the [OI] 6300A emission line is consistent with being emitted from the inner disk. The model can also reproduce the line flux of H2 v=1-0 S(1) at 2.122 mic. An additional high-velocity component of the [OI] 6300A emission line, however, points to the existence of an additional jet/outflow of low velocity (40 - 65) km/s with mass loss rate ~1.E-9 Msun/yr. In relation to our low estimations of the disk mass, such a mass loss rate suggests a disk lifetime of only ~(0.05 - 3) Myr, substantially shorter than the cluster age. The evolutionary state of this unusual protoplanetary disk is discussed.Comment: accepted by Astronomy & Astrophysics (18 pages, 11 figures and 7 tables). Additional 9-page appendix with 6 figures, 3 tables and 37 equation

A survey of the state-of-the-art techniques for cognitive impairment detection in the elderly

With a growing number of elderly people in the UK, more and more of them suffer from various kinds of cognitive impairment. Cognitive impairment can be divided into different stages such as mild cognitive impairment (MCI) and severe cognitive impairment like dementia. Its early detection can be of great importance. However, it is challenging to detect cognitive impairment in the early stage with high accuracy and low cost, when most of the symptoms may not be fully expressed. This survey paper mainly reviews the state of the art techniques for the early detection of cognitive impairment and compares their advantages and weaknesses. In order to build an effective and low-cost automatic system for detecting and monitoring the cognitive impairment for a wide range of elderly people, the applications of computer vision techniques for the early detection of cognitive impairment by monitoring facial expressions, body movements and eye movements are highlighted in this paper. In additional to technique review, the main research challenges for the early detection of cognitive impairment with high accuracy and low cost are analysed in depth. Through carefully comparing and contrasting the currently popular techniques for their advantages and weaknesses, some important research directions are particularly pointed out and highlighted from the viewpoints of the authors alone

Non-motor symptom burden in patients with Parkinson's disease with impulse control disorders and compulsive behaviours : results from the COPPADIS cohort

The study was aimed at analysing the frequency of impulse control disorders (ICDs) and compulsive behaviours (CBs) in patients with Parkinson's disease (PD) and in control subjects (CS) as well as the relationship between ICDs/CBs and motor, nonmotor features and dopaminergic treatment in PD patients. Data came from COPPADIS-2015, an observational, descriptive, nationwide (Spain) study. We used the validated Questionnaire for Impulsive-Compulsive Disorders in Parkinson's Disease-Rating Scale (QUIP-RS) for ICD/CB screening. The association between demographic data and ICDs/CBs was analyzed in both groups. In PD, this relationship was evaluated using clinical features and treatment-related data. As result, 613 PD patients (mean age 62.47 ± 9.09 years, 59.87% men) and 179 CS (mean age 60.84 ± 8.33 years, 47.48% men) were included. ICDs and CBs were more frequent in PD (ICDs 12.7% vs. 1.6%, p < 0.001; CBs 7.18% vs. 1.67%, p = 0.01). PD patients had more frequent previous ICDs history, premorbid impulsive personality and antidepressant treatment (p < 0.05) compared with CS. In PD, patients with ICDs/CBs presented younger age at disease onset, more frequent history of previous ICDs and premorbid personality (p < 0.05), as well as higher comorbidity with nonmotor symptoms, including depression and poor quality of life. Treatment with dopamine agonists increased the risk of ICDs/CBs, being dose dependent (p < 0.05). As conclusions, ICDs and CBs were more frequent in patients with PD than in CS. More nonmotor symptoms were present in patients with PD who had ICDs/CBs compared with those without. Dopamine agonists have a prominent effect on ICDs/CBs, which could be influenced by dose

High resolution spectroscopic characterization of the FGK stars in the Solar neighborhood

We present the most recent results of our ongoing long-term high resolution spectroscopic study of nearby (d ≤ 25 pc) FGK stars which aim is to characterize the local properties of the Galaxy, in particular the star-formation history. A through analysis has been carried out for 253 cool stars in the solar neighborhood. This includes radial and rotational velocities determinations, chromospheric activity levels inference, kinematic analysis, and age estimates. This study does not only shed new light on the issue of stellar formation history but also contributes to any present or future mission aiming to detect extra-solar planets. Exo-planets are likely to be found orbiting around nearby cool stars and their detection and characterization is highly dependent on the precise determination of fundamental stellar parameters such as age, activity levels. Therefore, our study is of paramount importance to ensure the success of any such mission

FR Cnc revisited : photometry, polarimetry and spectroscopy

This paper is part of a multiwavelength study aimed at using complementary photometric, polarimetric and spectroscopic data to achieve an understanding of the activity process in late-type stars. Here, we present the study of FR Cnc, a young, active and spotted star. We performed analysis of All Sky Automated Survey 3 (ASAS-3) data for the years 2002-08 and amended the value of the rotational period to be 0.826518 d. The amplitude of photometric variations decreased abruptly in the year 2005, while the mean brightness remained the same, which was interpreted as a quick redistribution of spots. BVRC and I-C broad-band photometric calibration was performed for 166 stars in FR Cnc vicinity. The photometry at Terskol Observatory shows two brightening episodes, one of which occurred at the same phase as the flare of 2006 November 23. Polarimetric BVR observations indicate the probable presence of a supplementary source of polarization. We monitored FR Cnc spectroscopically during the years 2004-08. We concluded that the radial velocity changes cannot be explained by the binary nature of FR Cnc. We determined the spectral type of FR Cnc as K7V. Calculated galactic space-velocity components (U, V, W) indicate that FR Cnc belongs to the young disc population and might also belong to the IC 2391 moving group. Based on Li I lambda 6707.8 measurement, we estimated the age of FR Cnc to be between 10 and 120 Myr. Doppler tomography was applied to create a starspot image of FR Cnc. We optimized the goodness of fit to the deconvolved profiles for axial inclination, equivalent width and v sin i, finding v sin i = 46.2 kms(-1) and i = 55 degrees.. We also generated a synthetic V-band light curve based on Doppler imaging that makes simultaneous use of spectroscopic and photometric data. This synthetic light curve displays the same morphology and amplitude as the observed one. The starspot distribution of FR Cnc is also of interest since it is one of the latest spectral types to have been imaged. No polar spot was detected on FR Cnc.Peer reviewe