Evaluation of amendments in the rehabilitation of sulfide mine tailings from São Domingos

RAMIRAN International ConferenceThe São Domingos mining area is located in the Iberian Pyrite Belt, SE Portugal, and represents a serious environmental hazard (Matos and Martins, 2006). Exploitation dated back to pre-roman and roman times with extraction of Ag, Au and Cu exploitation, mainly in the gossan (resulting from the ore weathering). The intense exploitation started in the middle of 19th century, both in the gossan and sulfide ore-containing Cu, Zn, As and Pb, and lasted until 1960, with the exhaustion of the ore (Quental et al., 2002). Different types of waste materials were left: gossan, host rocks (volcanic with shales, and shales), roman and modern slags, smelting ashes and brittle and blocks of pyrite (Matos, 2004; Álvarez-Valero et al., 2008). All sulfide mine wastes are typically heterogeneous and contain high amounts of trace elements, acidic pH and small contents of organic matter and nutrients. The large dumps containing pyrite and other metal sulfides generate, by oxidation, acidic mine drainage (AMD) which increases the availability of trace elements for microorganisms and plants in the surrounding soils. All of these characteristics contribute towards a system that is barely capable of supporting the establishment or survival of plants. The use of amendments and spontaneous colonization (vegetation) from mining areas (phytostabilization) are cost-effective and environmentally sustainable methods to rehabilitate these contaminated and degraded areas even in arid and semi-arid conditions (Tordoff et al., 2000; Mendez and Maier, 2008). Thus, the preparation of Technosols from mixtures of organic and inorganic wastes can be an attractive option to rehabilitate mining areas because they can improve physical, chemical and biological properties contributing, at the same time, towards a strategy of wastes valorisation (Macías, 2004). The use of mixtures composed of residues with different C:N ratios can be used to manipulate the rate of mineralization. Nevertheless, the amendments used should also promote other soil functions (Arbestain et al., 2008). The objective of this study was to evaluate the influence of cost-effective organic and inorganic amendments, available in the region, in the rehabilitation of sulfide materials from the São Domingos mine are

Cistus monspeliensis L. as a potential species for rehabilitation of soils with multielemental contamination under Mediterranean conditions

Conservation Biolog

Lead uptake capacity of Cistus plants growing in mining areas

Mining activity can give rise to large quantities of chemically unstable waste rocks and tailings which can drain potential environmentally harmful lixiviates rich in chemical elements. Plants growing in such environments can minimize chemical, physical and visual negative impacts. To evaluate lead absorption by native Cistus growing in Braçal (NW Portugal) and São Domingos (SE Portugal) mining areas, Cistus inflatus (aerial parts) and Cistus ladanifer (leaves) were analysed by instrumental neutron activation analysis and atomic absorption spectrometric, respectively, after acid digestions. Soils were characterized by pH(H2O), organic carbon, cation exchange capacity, free Fe and Mn, total nitrogen, and assimilable K and P. Total lead was determined in soils (fraction < 2 mm) by inductively coupled plasma emission spectrometry after acid digestion, and Pb available fraction was determined by atomic absorption spectrometric and inductively coupled plasma mass spectroscopy after calcium nitrate extraction. High concentrations of total Pb are present in both mining areas soils. However, in São Domingos soils the Pb available fraction is smaller (8.3–67.3 mg kg-1) than in Braçal (276–6175 mg kg-1). Cistus inflatus aerial parts contain higher concentrations of lead (49.5–217 mg kg-1) than Cistus ladanifer leaves (40.4–48.6 mg kg-1). Both Cistus, showing no external stress signals, are well adapted to these contaminated soils with low nutrient and high Pb contents. It is apparent that these plants can be used for phytostabilization in similar mining areas

The Combination of Gefitinib With ATRA and ATO Induces Myeloid Differentiation in Acute Promyelocytic Leukemia Resistant Cells

In approximately 15% of patients with acute myeloid leukemia (AML), total and phosphorylated EGFR proteins have been reported to be increased compared to healthy CD34(+) samples. However, it is unclear if this subset of patients would benefit from EGFR signaling pharmacological inhibition. Pre-clinical studies on AML cells provided evidence on the pro-differentiation benefits of EGFR inhibitors when combined with ATRA or ATO in vitro. Despite the success of ATRA and ATO in the treatment of patients with acute promyelocytic leukemia (APL), therapy-associated resistance is observed in 5-10% of the cases, pointing to a clear need for new therapeutic strategies for those patients. In this context, the functional role of EGFR tyrosine-kinase inhibitors has never been evaluated in APL. Here, we investigated the EGFR pathway in primary samples along with functional in vitro and in vivo studies using several APL models. We observed that total and phosphorylated EGFR (Tyr992) was expressed in 28% and 19% of blast cells from APL patients, respectively, but not in healthy CD34(+) samples. Interestingly, the expression of the EGF was lower in APL plasma samples than in healthy controls. The EGFR ligand AREG was detected in 29% of APL patients at diagnosis, but not in control samples. In vitro, treatment with the EGFR inhibitor gefitinib (ZD1839) reduced cell proliferation and survival of NB4 (ATRA-sensitive) and NB4-R2 (ATRA-resistant) cells. Moreover, the combination of gefitinib with ATRA and ATO promoted myeloid cell differentiation in ATRA- and ATO-resistant APL cells. In vivo, the combination of gefitinib and ATRA prolonged survival compared to gefitinib- or vehicle-treated leukemic mice in a syngeneic transplantation model, while the gain in survival did not reach statistical difference compared to treatment with ATRA alone. Our results suggest that gefitinib is a potential adjuvant agent that can mitigate ATRA and ATO resistance in APL cells. Therefore, our data indicate that repurposing FDA-approved tyrosine-kinase inhibitors could provide new perspectives into combination therapy to overcome drug resistance in APL patients

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02