Variation in Loblolly Pine Ring Microfibril Angle in the Southeastern United States

The effect of physiographic region on microfibril angle (MFA) in loblolly pine (Pinus taeda L.) in the southern United States was evaluated. MFA was determined at 1.4, 4.6, 7.6, 10.7, and 13.7 m up the stem of 59 trees, representing five physiographic regions. A nonlinear mixed-effects model was developed to test for regional differences in the initial value of MFA, the rate at which MFA changes with ring number from pith, and the lower bound of MFA achieved. Our results suggest that the parameters of interest differ significantly by region. It was found that MFA differs significantly between the South Atlantic, Gulf, and Hilly regions, compared to the North Atlantic and Piedmont regions. The initial value of MFA was found to be smaller in the Piedmont compared to all other regions. Similarly, the rate at which MFA changes with ring number was found to be significantly smaller in the North Atlantic and Piedmont regions. A test of the lower bound of MFA indicates that the Piedmont region has a significantly larger lower asymptote. These results combined indicate that overall, MFA values are larger in the North Atlantic and Piedmont regions

Effect of mid-rotation fertilization on stiffness and strength of loblolly pine wood

Trees sampled from a loblolly pine mid-rotation fertilization trial were used in this study. The study was laid out in a randomized complete block design with four levels of nitrogen fertilizer as treatments: control (000N), 112 (112N), 224 (224N), and 336 (336N) kg/ha of nitrogen, with each treatment replicated in 4 blocks. Two trees were destructively sampled from each plot giving a total of 32 trees. Bolts 0.6 m in length were collected from each tree (3 bolts per tree); with the midpoint of each bolt at heights of 2.4, 7.3 and 12.2 m from the base of the tree (each bolt represented the midpoint of standard 4.9 m saw logs). Static bending samples with dimensions 25 by 25 by 406 mm (radial, tangential and longitudinal dimensions respectively) were cut from the bolts that included the 25 mm of wood produced immediately following fertilization. Data on modulus of elasticity (MOE, stiffness) and modulus of rupture (MOR, strength) were collected from clear static bending samples. Based on the analysis of variance, no significant treatment effect was observed on MOE and MOR. However, MOE and MOR decreased in wood produced immediately after fertilization for trees which received the highest level of fertilization (336N). A decreasing trend in MOE and MOR with height was also present.Keywords: Loblolly pine, Fertilization, Strength, Stiffnes

Growth and wood properties of genetically improved loblolly pine: propagation type comparison and genetic parameters

The use of clonal varieties in forestry offers great potential to improve growth traits (quantity) and wood properties (quality) of loblolly pine (Pinus taeda L.). Loblolly pine trees established via somatic embryogenesis (clones), full-sib zygotic crosses, and half-sib zygotic open-pollinated families were sampled to identify variation in growth and wood properties among and within clonal lines and zygotic controls. Increment cores 5 mm in diameter were collected at age 4 from a total of 2615 trees. Growth properties (diameter at 1.4 m and total tree height) and wood properties (whole-core density, latewood and earlywood density, and latewood percent) were measured for each tree sampled in the study. Overall, growth properties were better for full-sib seedling than for clonal lines, whereas wood density was higher for clonal lines than full-sib and open-pollinated seedlings. However, there were clonal lines with better growth and higher wood density. Clonal repeatability of both growth and wood properties across sampled sites and genetic correlations between growth and wood traits were determined, with higher repeatability observed for wood traits compared with growth traits. Significant genetic correlations were observed for tree height and wood properties, whereas weak correlations were observed for diameter and wood properties

An optrode array for spatiotemporally precise large-scale optogenetic stimulation of deep cortical layers in non-human primates

Optogenetics has transformed studies of neural circuit function, but remains challenging to apply in large brains, such as those of non-human primates (NHPs). A major challenge is delivering intense, spatiotemporally precise, patterned photostimulation across large volumes in deep tissue. Such stimulation is critical, for example, to modulate selectively deep-layer corticocortical feedback projections. To address this unmet need, we have developed the Utah Optrode Array (UOA), a 10×10 glass needle waveguide array fabricated atop a novel opaque optical interposer then bonded to an electrically addressable μLED array. In vivo experiments with the UOA demonstrated large-scale, spatiotemporally precise, activation of deep circuits in monkey cortex. Specifically, the UOA permitted both focal (confined to single layers/columns), and widespread (multiple layers/columns) optogenetic activation of deep layer neurons, simply by varying the number of activated μLEDs and/or the irradiance. Thus, the UOA represents a powerful optoelectronic device for targeted manipulation of deep-layer circuits in NHP models.Competing Interest StatementThe authors have declared no competing interest

An optrode array for spatiotemporally-precise large-scale optogenetic stimulation of deep cortical layers in non-human primates

Optogenetics has transformed studies of neural circuit function, but remains challenging to apply to non-human primates (NHPs). A major challenge is delivering intense, spatiotemporally-precise, patterned photostimulation across large volumes in deep tissue. Such stimulation is critical, for example, to modulate selectively deep-layer corticocortical feedback circuits. To address this need, we have developed the Utah Optrode Array (UOA), a 10×10 glass needle waveguide array fabricated atop a novel opaque optical interposer, and bonded to an electrically addressable µLED array. In vivo experiments with the UOA demonstrated large-scale, spatiotemporally precise, activation of deep circuits in NHP cortex. Specifically, the UOA permitted both focal (confined to single layers/columns), and widespread (multiple layers/columns) optogenetic activation of deep layer neurons, as assessed with multi-channel laminar electrode arrays, simply by varying the number of activated µLEDs and/or the irradiance. Thus, the UOA represents a powerful optoelectronic device for targeted manipulation of deep-layer circuits in NHP models

Studying neuroanatomy using MRI

The study of neuroanatomy using imaging enables key insights into how our brains function, are shaped by genes and environment, and change with development, aging, and disease. Developments in MRI acquisition, image processing, and data modelling have been key to these advances. However, MRI provides an indirect measurement of the biological signals we aim to investigate. Thus, artifacts and key questions of correct interpretation can confound the readouts provided by anatomical MRI. In this review we provide an overview of the methods for measuring macro- and mesoscopic structure and inferring microstructural properties; we also describe key artefacts and confounds that can lead to incorrect conclusions. Ultimately, we believe that, though methods need to improve and caution is required in its interpretation, structural MRI continues to have great promise in furthering our understanding of how the brain works

Margarita de Sossa, Sixteenth-Century Puebla de los Ángeles, New Spain (Mexico)

Margarita de Sossa’s freedom journey was defiant and entrepreneurial. In her early twenties, still enslaved in Portugal, she took possession of her body; after refusing to endure her owner’s sexual demands, he sold her, and she was transported to Mexico. There, she purchased her freedom with money earned as a healer and then conducted an enviable business as an innkeeper. Sossa’s biography provides striking insights into how she conceptualized freedom in terms that included – but was not limited to – legal manumission. Her transatlantic biography offers a rare insight into the life of a free black woman (and former slave) in late sixteenth-century Puebla, who sought to establish various degrees of freedom for herself. Whether she was refusing to acquiesce to an abusive owner, embracing entrepreneurship, marrying, purchasing her own slave property, or later using the courts to petition for divorce. Sossa continued to advocate on her own behalf. Her biography shows that obtaining legal manumission was not always equivalent to independence and autonomy, particularly if married to an abusive husband, or if financial successes inspired the envy of neighbors

Exploration of Victoria Crater by the Mars Rover Opportunity

The Mars rover Opportunity has explored Victoria crater, a ~750-meter eroded impact crater formed in sulfate-rich sedimentary rocks. Impact-related stratigraphy is preserved in the crater walls, and meteoritic debris is present near the crater rim. The size of hematite-rich concretions decreases up-section, documenting variation in the intensity of groundwater processes. Layering in the crater walls preserves evidence of ancient wind-blown dunes. Compositional variations with depth mimic those ~6 kilometers to the north and demonstrate that water-induced alteration at Meridiani Planum was regional in scope.Organismic and Evolutionary Biolog

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure