Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Multiple criteria decision analysis for sanitation selection in South African municipalities

A multiple criteria decision analysis (MCDA) was developed for the selection of sanitation systems. This decision support system was aimed at assisting municipal engineers to design and implement sustainable solutions to meet a municipality’s obligation to provide free basic sanitation (FBS). Multi-attribute value theory (MAVT) was selected as the method most suited to the problem under consideration. Criteria which determine the sustainability of sanitation were selected from the literature and a spreadsheet-based MCDA with stakeholder and expert user interfaces was developed. Stakeholders determine the weighting of each indicator and expert users determine the values to be entered for the alternatives against each indicator. The partial values are aggregated using a weighted sum function. Research carried out into the implementation of FBS by the eThekwini Municipality that includes the city of Durban was analysed. This informed the allocation of indicator values to the sanitation alternatives under consideration: ventilated improved pit latrines (VIPs) and urine diversion dehydrating toilets (UDDTs). An innovative scenario analysis method was used to determine the effect of different weightings and/or values, representing changes in stakeholder involvement, resource recovery and political support for ecological sanitation. The MCDA was found to provide a guiding framework for municipal engineers in their efforts to implement sustainable sanitation. The process of deriving values for the MCDA is likely to prove even more useful than the overall value scores of the options under consideration

Understanding the Stable Isotope Composition of Biosphere-Atmosphere CO 2 Exchange

Stable isotopes of atmospheric carbon dioxide (CO2) contain a wealth of information regarding biosphere-atmosphere interactions. The carbon isotope ratio of CO2 (δ13C) reflects the terrestrial carbon cycle including processes of photosynthesis, respiration, and decomposition. The oxygen isotope ratio (δ18O) reflects terrestrial carbon and water coupling due to CO2-H2O oxygen exchange. Isotopic CO2 measurements, in combination with ecosystem-isotopic exchange models, allow for the quantification of patterns and mechanisms regulating terrestrial carbon and water cycles, as well as for hypothesis development, data interpretation, and forecasting. Isotopic measurements and models have evolved significantly over the past two decades, resulting in organizations that promote model-measurement networks, e.g., the U.S. National Science Foundation's Biosphere-Atmosphere Stable Isotope Network, the European Stable Isotopes in Biosphere-Atmosphere Exchange Network, and the U.S. National Environmental Observatory Network

Understanding the stable isotope composition of biosphere-atmosphere CO2 exchange

Stable isotopes of atmospheric carbon dioxide (CO2) contain a wealth of information regarding biosphere-atmosphere interactions. The carbon isotope ratio of CO2 (δ13C) reflects the terrestrial carbon cycle including processes of photosynthesis, respiration, and decomposition. The oxygen isotope ratio (δ18O) reflects terrestrial carbon and water coupling due to CO2-H2O oxygen exchange. Isotopic CO2 measurements, in combination with ecosystem-isotopic exchange models, allow for the quantification of patterns and mechanisms regulating terrestrial carbon and water cycles, as well as for hypothesis development, data interpretation, and forecasting. Isotopic measurements and models have evolved significantly over the past two decades, resulting in organizations that promote model-measurement networks, e.g., the U.S. National Science Foundation's Biosphere-Atmosphere Stable Isotope Network, the European Stable Isotopes in Biosphere-Atmosphere Exchange Network, and the U.S. National Environmental Observatory Network