245 research outputs found
Glosando las confesiones de los hermanos Ávila. Discurso e identidad en la Nueva España a finales del siglo XVI
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Explaining Sentinel 2-based dNBR and RdNBR variability with reference data from the bird’s eye (UAS) perspective
Get PDFCharacterizing the spatial variability of the severity of wildfires is important to assess ecological and economic consequences and to coordinate mitigation strategies. Vegetation indices such as the differenced Normalized Burn Ratio (dNBR) have become a standard tool to assess burn or fire severity across larger areas and are being used operationally. Despite the frequent application of dNBR-like vegetation indices, it is not yet fully understood which variables exactly drive the variability in dNBR observed by multispectral satellites. One reason for this is the lack of high quality prefire information about vegetation structure and composition. Consequently, the influence of prefire vegetation composition and other potentially influential variables such as cast shadows has hardly been examined. Here, we use very high resolution Unmanned Aerial System (UAS) orthoimages collected briefly before and after the large wildfires in Central Chile in the fire season 2016/2017 to derive variables related to the pre- and postfire landscape composition and structure. The variables are used as predictors in Generalized Additive Models (GAM) to explain the spatial variability in dNBR and RdNBR pixel values as observed by Sentinel-2. Our models explain more than 80% and 75% of the variability in dNBR and RdNBR values, respectively, using a sparse set of five predictors. The results suggest that in our study area the largest fraction of variability in Sentinel-2 based dNBR and RdNBR values can be explained by variables related to the fraction of consumed canopy cover while the vegetation composition before the fire does not have a large influence on dNBR and RdNBR.
Our results further suggest that cast-shadows of snags and standing dead trees with remaining crown structure have a notable influence on the dNBR signal which may have been underestimated so far. We conclude that spatially continuous, very high spatial resolution data from UAS can be a valuable data source for an improved understanding of the exact meaning of common vegetation index products, operationally used for monitoring the environment
Mannan-binding lectin and procalcitonin measurement for prediction of postoperative infection
Get PDFINTRODUCTION: Postoperative infection is a major cause of morbidity and mortality. We investigated two serum markers for their ability to identify patients at risk for postoperative infection. Mannan-binding lectin (MBL) is a central molecule of the innate immune system and MBL deficiency is known to predispose to infection. Procalcitonin (PCT) is a sensitive marker for bacterial infection. METHODS: We investigated 162 patients undergoing elective surgery for cancer of the gastrointestinal tract. Patients were classified as having no complications (group A), having infection for unknown reason (group B) or having sepsis after events like aspiration or anastomotic leakage (group C). Analysis was done pre- and postoperatively for serum levels of MBL, PCT and C-reactive-protein. DNA was preoperatively sampled and stored and later analysed for genetic polymorphisms of MBL. RESULTS: The preoperative serum levels of MBL were significantly lower in group B patients than in group A patients (1332 ± 466 ng/ml versus 2523 ± 181 ng/ml). PCT measured on day one post-surgery was significantly higher in group B patients than in group A (3.33 ± 1.08 ng/ml versus 1.38 ± 0.17 ng/ml). Patients with an aberrant MBL genotype had a significantly higher risk of postoperative infections than wild-type carriers (p < 0.05). CONCLUSION: Preoperative MBL and early postoperative PCT measurement may help identify patients at risk for postoperative infection
Super Resolution Fluorescence Microscopy and Tracking of Bacterial Flotillin (Reggie) Paralogs Provide Evidence for Defined-Sized Protein Microdomains within the Bacterial Membrane but Absence of Clusters Containing Detergent-Resistant Proteins
Get PDFBiological membranes have been proposed to contain microdomains of a specific lipid composition, in which distinct groups of proteins are clustered. Flotillin-like proteins are conserved between pro—and eukaryotes, play an important function in several eukaryotic and bacterial cells, and define in vertebrates a type of so-called detergent-resistant microdomains. Using STED microscopy, we show that two bacterial flotillins, FloA and FloT, form defined assemblies with an average diameter of 85 to 110 nm in the model bacterium Bacillus subtilis. Interestingly, flotillin microdomains are of similar size in eukaryotic cells. The soluble domains of FloA form higher order oligomers of up to several hundred kDa in vitro, showing that like eukaryotic flotillins, bacterial assemblies are based in part on their ability to self-oligomerize. However, B. subtilis paralogs show significantly different diffusion rates, and consequently do not colocalize into a common microdomain. Dual colour time lapse experiments of flotillins together with other detergent-resistant proteins in bacteria show that proteins colocalize for no longer than a few hundred milliseconds, and do not move together. Our data reveal that the bacterial membrane contains defined-sized protein domains rather than functional microdomains dependent on flotillins. Based on their distinct dynamics, FloA and FloT confer spatially distinguishable activities, but do not serve as molecular scaffolds
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Horizontal transport affecting trace gas seasonality in the Tropical Tropopause Layer (TTL)
Get PDFWe analyze horizontal transport from midlatitudes into the tropics (in-mixing) and its impact on seasonal variations of ozone, carbon monoxide and water vapor in the Tropical Tropopause Layer (TTL). For this purpose, we use three-dimensional backward trajectories, driven by ECMWF ERA-Interim winds, and a conceptual one-dimensional model of the chemical composition of the TTL. We find that the fraction of in-mixed midlatitude air shows an annual cycle with maximum during NH summer, resulting from the superposition of two inversely phased annual cycles for in-mixing from the NH and SH, respectively. In-mixing is driven by the monsoonal upper-level anticyclonic circulations. This circulation pattern is dominated by the Southeast Asian summer monsoon and, correspondingly, in-mixing shows an annual cycle. The impact of in-mixing on TTL mixing ratios depends on the in-mixed fraction of midlatitude air and on the meridional gradient of the particular species. For CO the meridional gradient and consequently the effect of in-mixing is weak. For water vapor, in-mixing effects are negligible. For ozone, the meridional gradient is large and the contribution of in-mixing to the ozone maximum during NH summer is about 50%. This in-mixing contribution is not sensitive to the tropical ascent velocity, which is about 40% too fast in ERA-Interim. As photochemically produced ozone in the TTL shows no distinct summer maximum, the ozone annual anomaly in the upper TTL turns out to be mainly forced by in-mixing of ozone-rich extratropical air during NH summer
The Rise and Fall of a Change from Below in early modern Spanish: the Periphrasis Deber De + Infinitive in Texts of Linguistic Immediacy
Get PDFThis paper deals with the patterns of variation and change undergone by a syntactic variable in early modern Spanish grammar, namely the alternation between deber ‘have to, must/should’ and deber de + infinitive ‘have to, must/should’ as a modal periphrasis. Based on a 1,500,000 word corpus of immediacy text (private letters, memories) the results of this variationist study suggest that, throughout the 16th century, but more especially during its second half, the prepositional periphrasis gradually became more common, above all in stylistic contexts predominated by the spontaneity and proximity of the relationships between the interlocutors. It was also more frequently found in contexts involving members of northern speech communities, particularly males, the young, and the middle-low social strata, the incidence being especially high at the points where some of these groups intersect. All this suggests a change from below in the Golden Age period, which reached considerable dimensions in a relatively short time, but was destined to stagnate and later decline just as quickly in the centuries that followed as a result of some structural features, such as the special ‘visibility’ of the preposition, which could have led to the stigmatization of the periphrasis in a similar way to what happened in other Spanish syntactic phenomena
Reconstitution of Mammary Gland Development In Vitro: Requirement of c-met and c-erbB2 Signaling for Branching and Alveolar Morphogenesis
Get PDFWe have established a cell culture system that reproduces morphogenic processes in the developing mammary gland. EpH4 mouse mammary epithelial cells cultured in matrigel form branched tubules in the presence of hepatocyte growth factor/scatter factor (HGF/SF), the ligand of the c-met tyrosine kinase receptor. In contrast, alveolar structures are formed in the presence of neuregulin, a ligand of c-erbB tyrosine kinase receptors. These distinct morphogenic responses can also be observed with selected human mammary carcinoma tissue in explant culture. HGF/SF-induced branching was abrogated by the PI3 kinase inhibitors wortmannin and LY294002. In contrast, neuregulin- induced alveolar morphogenesis was inhibited by the MAPK kinase inhibitor PD98059. The c-met–mediated response could also be evoked by transfection of a c-met specific substrate, Gab1, which can activate the PI3 kinase pathway. An activated hybrid receptor that contained the intracellular domain of c-erbB2 receptor suffices to induce alveolar morphogenesis, and was observed in the presence of tyrosine residues Y1028, Y1144, Y1201, and Y1226/27 in the substrate-binding domain of c-erbB2. Our data demonstrate that c-met and c-erbB2 signaling elicit distinct morphogenic programs in mammary epithelial cells: formation of branched tubules relies on a pathway involving PI3 kinase, whereas alveolar morphogenesis requires MAPK kinase
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The extratropical upper troposphere and lower stratosphere
Get PDFThe extratropical upper troposphere and lower stratosphere (Ex-UTLS) is a transition region between the stratosphere and the troposphere. The Ex-UTLS includes the tropopause, a strong static stability gradient and dynamic barrier to transport. The barrier is reflected in tracer profiles. This region exhibits complex dynamical, radiative, and chemical characteristics that place stringent spatial and temporal requirements on observing and modeling systems. The Ex-UTLS couples the stratosphere to the troposphere through chemical constituent transport (of, e.g., ozone), by dynamically linking the stratospheric circulation with tropospheric wave patterns, and via radiative processes tied to optically thick clouds and clear-sky gradients of radiatively active gases. A comprehensive picture of the Ex-UTLS is presented that brings together different definitions of the tropopause, focusing on observed dynamical and chemical structure and their coupling. This integral view recognizes that thermal gradients and dynamic barriers are necessarily linked, that these barriers inhibit mixing and give rise to specific trace gas distributions, and that there are radiative feedbacks that help maintain this structure. The impacts of 21st century anthropogenic changes to the atmosphere due to ozone recovery and climate change will be felt in the Ex-UTLS, and recent simulations of these effects are summarized and placed in context
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