Determination of the antimicrobial susceptibility pattern of enterobacteriaceae in a tertiary health center of Northeastern Nigeria.

Gram negative resistance particularly amongst species of has emerged as a worldwide threat affecting the efficacy of our commonly used antimicrobial agents. These threats affect the quality of care and results in prolonged hospitalization and increase in health care cost. We set out to evaluate the occurrence and profile of antimicrobial resistance from clinical isolates of in a tertiary health center of north-eastern, Nigeria. Method: Confirmed species of isolated from 225 patients that were admitted in various units of University of Maiduguri Teaching Hospital (UMTH) Maiduguri were tested for susceptibility to 6 antimicrobial agents; ampicillin (10µg), aztreonam (30µg), gentamicin (10µg), ceftriaxone (30µg), ciprofloxacin (5µg) and amoxicillin/clavulanic acid (20/10µg) using the clinical and laboratory standards institute (CLSI) breakpoints. Results: A total of 225 clinical variants of were isolated during the study period. The specie with the highest proportion was with 73(32.4%) and this was followed closely by with 61(27.1%). The result of the susceptibility testing on this species of showed ampicillin (10µg) with the highest resistance of 118(52.4%) while aztreonam(30µg) has the lowest resistance of 58(25.8%). Conclusion: There is the existence of widespread resistance to mostly the beta lactam agents among species of with the exception of Aztreonam. Irrational use of antibiotics must be discouraged so as to reduce this emerging threat. Stringent infection control and antibiotic stewardship programshould be established and sustained in our hospitals nationwide

microRNAs and EMT in mammary cells and breast cancer

MicroRNAs are master regulators of gene expression in many biological and pathological processes, including mammary gland development and breast cancer. The differentiation program termed the epithelial to mesenchymal transition (EMT) involves changes in a number of microRNAs. Some of these microRNAs have been shown to control cellular plasticity through the suppression of EMT-inducers or to influence cellular phenotype through the suppression of genes involved in defining the epithelial and mesenchymal cell states. This has led to the suggestion that microRNAs maybe a novel therapeutic target for the treatment of breast cancer. In this review, we will discuss microRNAs that are involved in EMT in mammary cells and breast cancer.Josephine A. Wright, Jennifer K. Richer and Gregory J. Goodal

microRNAs in breast cancer: regulatory roles governing the hallmarks of cancer

A large number of etiological factors and the complexity of breast cancers present challenges for prevention and treatment. Recently, the emergence of microRNAs (miRNAs) as cancer biomarkers has added an extra dimension to the ‘molecular signatures’ of breast cancer. Bioinformatic analyses indicate that each miRNA can regulate hundreds of target genes and could serve functionally as ‘oncogenes’ or ‘tumour suppressor’ genes, and co-ordinate multiple cellular processes relevant to cancer progression. A number of studies have shown that miRNAs play important roles in breast tumorigenesis, metastasis, proliferation and differentiation of breast cancer cells. This review provides a comprehensive overview of miRNAs with established functional relevance in breast cancer, their established target genes and resulting cellular phenotype. The role and application of circulating miRNAs in breast cancer is also discussed. Furthermore, we summarize the role of miRNAs in the hallmarks of breast cancer, as well as the possibility of using miRNAs as potential biomarkers for detection of breast cancer