Protection of Rabbits against Rabbit Hemorrhagic Disease Virus by Immunization with the VP60 Protein Expressed in Plants with a Potyvirus-Based Vector

AbstractA new plum pox potyvirus (PPV)-based vector has been constructed for the expression of full-length individual foreign proteins. The foreign sequences are cloned between the NIb replicase and capsid protein (CP) cistrons. The heterologous protein is split from the rest of the potyviral polyprotein by cleavage at the site that originally separated the NIb and CP proteins and at an additional NIa protease recognition site engineered at its amino-terminal end. This vector (PPV–NK) has been used to clone different genes, engendering stable chimeras with practical applications. We have constructed a chimera expressing high levels of jellyfish green fluorescent protein, which can be very useful for the study of PPV molecular biology. The VP60 structural protein of rabbit hemorrhagic disease virus (RHDV) was also successfully expressed by making use of the PPV–NK vector. Inoculation of extracts from VP60-expressing plants induced a remarkable immune response against RHDV in rabbits, its natural host. Moreover, these animals were protected against a lethal challenge with RHDV

Post-Franco Theatre

In the multiple realms and layers that comprise the contemporary Spanish theatrical landscape, “crisis” would seem to be the word that most often lingers in the air, as though it were a common mantra, ready to roll off the tongue of so many theatre professionals with such enormous ease, and even enthusiasm, that one is prompted to wonder whether it might indeed be a miracle that the contemporary technological revolution – coupled with perpetual quandaries concerning public and private funding for the arts – had not by now brought an end to the evolution of the oldest of live arts, or, at the very least, an end to drama as we know it

Ácidos nucleicos que codifican para vacunas contra el virus del síndrome reproductivo y respiratorio porcino (PRRSV)

La presente invención se refiere a ácidos nucleicos que comprenden: secuencias de un virus de la gastroenteritis transmisible competente para la replicación (TGEV) y una secuencia que codifica para por 5 lo menos un epítopo neutralizante de ORF5 del virus del síndrome reproductivo y respiratorio porcino (PRRSV), incluyendo dicha secuencia un residuo de formación de puente disulfuro y habiéndose modificado para desactivar los sitios de glicosilación que interfieren en la inducción de anticuerpos.Peer reviewedConsejo Superior de Investigaciones Científicas (España), FORT DODGE VETERINARIA SAT3 Traducción de patente europe

Nucleic acids encoding prrsv gp5-ecto domain and m protein

The present invention relates to nucleic acids comprising: (a) a sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:I (sequence ofthe GP5 ecto-domain ofthe European strain ofPRRSV) or a sequence having at least 85% identity to SEQ ID NO: 1, which sequence includes at least one amino acid capable offorming a disulfide bridge; and (b) a sequence encoding a polypeptide comprising the amino acid sequence SEQ ID NO: 2 (sequence of the GP5 M protein of the European strain of PRRSV) or a sequence having at least 85% identity to SEQ ID NO: 2; (c) wherein the nucleic acid does not comprise a sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3 (sequence ofthe GP5 protein except the ecto-domain ofthe European strain of PRRSV) or a sequence having at least 80% identity to SEQ ID NO: 3. The present invention further provides respective nucleic acids from other strains of PRRSV and their use as a vaccine for the treatment of animals.Peer reviewedConsejo Superior de Investigaciones Científicas (España), PFIZER OLOT, S.L.U.A1 Solicitud de patente con informe sobre el estado de la técnic

Nucleic acid sequences encoding proteins capable of associating into avirus-like particle

Fecha de presentación internacional: 02.09.2005.- Titulares: Consejo Superior de Investigaciones Científicas (CSIC).- Fort Dodge Veterinaria, S.A.The present invention relates to nucleic acids comprising: (a) sequences of a replication competent transmissible gastroenteritis virus (TGEV), which sequences encode a TGEV replicase under the control of expression regulatory sequences so that expression of the replicase in a cell containing the nucleic acid will initiate replication of the nucleic acid and thus increase the number of nucleic acids in the cell; and (b) sequences encoding one or more proteins of a different virus wherein the one or more proteins are capable of associating into a virus-like particle (VLP) that does not contain any infectious nucleic acid. The present invention further relates to vectors, virus particles and host cells comprising these nucleic acids as well as their use for the preparation of vaccines, specifically for the preparation of vaccines.Peer reviewe

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

The objective of this work was to investigate the susceptibility of rabbits and mice experimentally inoculated with porcine circoviruses type 1 (PCV1) and type 2 (PCV2) to infection and development of disease and/or lesions. Forty six New Zealand rabbits and 50 ICR-CD1 mice were both divided into two groups comprising PCV1 and PCV2 inoculated animals, and a third group inoculated with non-infected cell culture medium. Rabbits were inoculated intranasally while mice were inoculated intraperitoneally. Clinical signs and body weights were recorded at the start of the experiment and at necropsy. Animals were bled, euthanised and necropsied at days 0, 3, 7, 10, 14 and 20 post-inoculation and samples were collected for histopathological, serological, in situ hybridisation and PCR analysis. No clinical signs or gross and microscopic lesions compatible with PCV2 infections such as those seen in pigs were observed. No presence of PCV2 nucleic acid was detected in rabbits and mice by in situ hybridisation. Only one mouse inoculated with PCV1 seroconverted on day 20 PI. PCV1 and PCV2 genome was detected in serum by PCR in mice inoculated with each porcine circovirus, while rabbits were negative for both viral types. These studies indicated that porcine circoviruses did not cause any disease or microscopic lesions in inoculated rabbits and mice during the experimental period. However, intraperitoneally inoculated mice might have harboured PCV2 in circulation without evidence of viral replication.Inoculation expérimentale de circovirus de type 1 (PCV1) et de type 2 (PCV2) chez le lapin et la souris. Le but de ce travail Etait d'étudier la sensibilité à l'infection et au développement de la maladie et/ou de lésions chez des lapins et des souris inoculés expérimentalement avec des circovirus porcins de type 1 (PCV1) et 2 (PCV2). Deux groupes de 46 lapins de Nouvelle-Zélande et 50 souris ICR-CD1 ont été chacun divisés en deux groupes comprenant des animaux inoculés avec PCV1 et PCV2, et un troisième groupe inoculé avec un milieu de culture non-infecté. Les lapins ont été inoculés par voie intranasale et les souris par voie intrapéritonéale. Les signes cliniques et le poids des animaux ont été enregistrés au début de l'expérience et au moment de l'autopsie. Aux jours 0, 3, 7, 10, 14 et 20 après inoculation, le sang des animaux a été prélevé, puis les animaux ont été euthanasiés et ont subi une autopsie ; des échantillons ont été prélevés pour des analyses histopathologique, sérologique, d'hybridation in situ et de PCR. Aucun signe clinique ni lésion macro- ou microscopique correspondant à ceux observés chez les porcs infectés par PCV2 n'ont été détectés. Aucune présence d'acide nucléique de PCV2 n'a été détectée chez les lapins et les souris par hybridation in situ. Seule une souris inoculée par PCV1 est devenue séropositive au jour 20 post-inoculation. Les génomes de PCV1 et PCV2 ont été détectés par PCR dans le sérum de souris inoculées par chacun des circovirus, alors que les lapins sont restés négatifs pour chacun des types. Ces études indiquent que les circovirus porcins n'ont provoqué aucune maladie ou lésion microscopique chez les lapins et les souris inoculés durant toute la durée de l'expérimentation. Cependant, les souris inoculées expérimentalement pourraient avoir hébergé du virus PCV2 dans la circulation sanguine sans signe de réplication virale