Estudio del proceso de cambio conceptual y la construcción del modelo científico precursor de ser vivo en niños de pre-escolar

La presente investigación estudia el cambio conceptual y la construcción de modelos científicos precursores en un contexto socioconstructivista con niños pequeños, utilizando una metodología cualitativa. Se analizan los cambios epistemológicos y ontológicos en las concepciones de los niños acerca de los seres vivos y cómo una estrategia didáctica basada en la construcción de un modelo científico precursor basado en propiedades biológicas los promueven. Los cambios en la comprensión de los niños fueron significativos en las dimensiones ontológicas y epistemológicas, presentando diferentes patones. Los segundos proporcionaron una mejor coherencia explicativa en su sistema conceptual contribuyendo, de esta forma, a los cambios ontológicos y promoviendo, a su vez, la construcción del modelo científico precursor de ser vivo

GJ 3236: a new bright, very low-mass eclipsing binary system discovered by the MEarth observatory

We report the detection of eclipses in GJ 3236, a bright (I = 11.6) very low mass binary system with an orbital period of 0.77 days. Analysis of light- and radial velocity curves of the system yielded component masses of 0.38 +/- 0.02 and 0.28 +/- 0.02 Msol. The central values for the stellar radii are larger than the theoretical models predict for these masses, in agreement with the results for existing eclipsing binaries, although the present 5% observational uncertainties limit the significance of the larger radii to approximately 1 sigma. Degeneracies in the light curve models resulting from the unknown configuration of surface spots on the components of GJ 3236 currently dominate the uncertainties in the radii, and could be reduced by obtaining precise, multi-band photometry covering the full orbital period. The system appears to be tidally synchronized and shows signs of high activity levels as expected for such a short orbital period, evidenced by strong Halpha emission lines in the spectra of both components. These observations probe an important region of mass-radius parameter space around the predicted transition to fully-convective stellar interiors, where there are a limited number of precise measurements available in the literature.Comment: 14 pages, 5 figures, 10 tables, emulateapj format. Accepted for publication in Ap

HI intensity mapping : a single dish approach

We discuss the detection of large scale HI intensity fluctuations using a single dish approach with the ultimate objective of measuring the Baryonic Acoustic Oscillations and constraining the properties of dark energy. We present 3D power spectra, 2D angular power spectra for individual redshift slices, and also individual line-of-sight spectra, computed using the S^3 simulated HI catalogue which is based on the Millennium Simulation. We consider optimal instrument design and survey strategies for a single dish observation at low and high redshift for a fixed sensitivity. For a survey corresponding to an instrument with T_sys=50 K, 50 feed horns and 1 year of observations, we find that at low redshift (z \approx 0.3), a resolution of 40 arc min and a survey of 5000 deg^2 is close to optimal, whereas at higher redshift (z \approx 0.9) a resolution of 10 arcmin and 500 deg^2 would be necessary. Continuum foreground emission from the Galaxy and extragalactic radio sources are potentially a problem. We suggest that it could be that the dominant extragalactic foreground comes from the clustering of very weak sources. We assess its amplitude and discuss ways by which it might be mitigated. We then introduce our concept for a single dish telescope designed to detect BAO at low redshifts. It involves an under-illumintated static 40 m dish and a 60 element receiver array held 90 m above the under-illuminated dish. Correlation receivers will be used with each main science beam referenced against an antenna pointing at one of the Celestial Poles for stability and control of systematics. We make sensitivity estimates for our proposed system and projections for the uncertainties on the power spectrum after 1 year of observations. We find that it is possible to measure the acoustic scale at z\approx 0.3 with an accuracy 2.4% and that w can be measured to an accuracy of 16%.Comment: 20 pages, 20 figures, submitted to MNRA

The Clusters AgeS Experiment (CASE). IV. Analysis of the Eclipsing Binary V69 in the Globular Cluster 47 Tuc

We use photometric and spectroscopic observations of the eclipsing binary V69-47 Tuc to derive the masses, radii, and luminosities of the component stars. Based on measured systemic velocity, distance, and proper motion, the system is a member of the globular cluster 47 Tuc. The system has an orbital period of 29.5 d and the orbit is slightly eccentric with e=0.056. We obtain Mp=0.8762 +- 0.0048 M(Sun), Rp=1.3148 +-0.0051 R(Sun), Lp=1.94 +- 0.21 L(Sun) for the primary and Ms=0.8588 +- 0.0060 M(Sun), Rs=1.1616 +- 0.0062 R(Sun), Ls=1.53 +- 0.17 L(Sun) for the secondary. These components of V69 are the first Population II stars with masses and radii derived directly and with an accuracy of better than 1%. We measure an apparent distance modulus of (m-M)v=13.35 +- 0.08 to V69. We compare the absolute parameters of V69 with five sets of stellar evolution models and estimate the age of V69 using mass-luminosity-age, mass-radius-age, and turnoff mass - age relations. The masses, radii, and luminosities of the component stars are determined well enough that the measurement of ages is dominated by systematic differences between the evolutionary models, in particular, the adopted helium abundance. By comparing the observations to Dartmouth model isochrones we estimate the age of V69 to be 11.25 +- 0.21(random) +- 0.85(systematic) Gyr assuming [Fe/H]=-0.70, [alpha/Fe]=0.4, and Y=0.255. The determination of the distance to V69, and hence to 47Tuc, can be further improved when infrared eclipse photometry is obtained for the variable.Comment: 49 pages, 15 figures, submitted to A

Near-infrared Linear Polarization of Ultracool Dwarfs

We report on near-infrared J- and H-band linear polarimetric photometry of eight ultracool dwarfs (two late-M, five L0-L7.5, and one T2.5) with known evidence for photometric variability due to dust clouds, anomalous red infrared colors, or low-gravity atmospheres. The polarimetric data were acquired with the LIRIS instrument on the William Herschel Telescope. We also provide mid-infrared photometry in the interval 3.4-24 micron for some targets obtained with Spitzer and WISE, which has allowed us to confirm the peculiar red colors of five sources in the sample. We can impose modest upper limits of 0.9% and 1.8% on the linear polarization degree for seven targets with a confidence of 99%. Only one source, 2MAS, J02411151-0326587 (L0), appears to be strongly polarized (P ~ 3%) in the J-band with a significance level of P/sigma_P ~ 10. The likely origin of its linearly polarized light and rather red infrared colors may reside in a surrounding disk with an asymmetric distribution of grains. Given its proximity (66 +/- 8 pc), this object becomes an excellent target for the direct detection of the disk.Comment: Accepted for publication in Ap

Charcot-Marie-Tooth disease type 2CC due to NEFH variants causes a progressive, non-length-dependent, motor-predominant phenotype

Objective: Neurofilaments are the major scaffolding proteins for the neuronal cytoskeleton, and variants in NEFH have recently been described to cause axonal Charcot-Marie-Tooth disease type 2CC (CMT2CC). Methods: In this large observational study, we present phenotype–genotype correlations on 30 affected and 3 asymptomatic mutation carriers from eight families. Results: The majority of patients presented in adulthood with motor-predominant and lower limb-predominant symptoms and the average age of onset was 31.0±15.1 years. A prominent feature was the development of proximal weakness early in the course of the disease. The disease progressed rapidly, unlike other Charcot-Marie-Tooth disease (CMT) subtypes, and half of the patients (53%) needed to use a wheelchair on average 24.1 years after symptom onset. Furthermore, 40% of patients had evidence of early ankle plantarflexion weakness, a feature which is observed in only a handful of CMT subtypes. Neurophysiological studies and MRI of the lower limbs confirmed the presence of a non-length-dependent neuropathy in the majority of patients. All families harboured heterozygous frameshift variants in the last exon of NEFH, resulting in a reading frameshift to an alternate open reading frame and the translation of approximately 42 additional amino acids from the 3' untranslated region (3′-UTR). Conclusions: This phenotype–genotype study highlights the unusual phenotype of CMT2CC, which is more akin to spinal muscular atrophy rather than classic CMT. Furthermore, the study will enable more informative discussions on the natural history of the disease and will aid in NEFH variant interpretation in the context of the disease’s unique molecular genetics

LSPM J1112+7626: detection of a 41-day M-dwarf eclipsing binary from the MEarth transit survey

We report the detection of eclipses in LSPM J1112+7626, which we find to be a moderately bright (I_C = 12.14 +/- 0.05) very low-mass binary system with an orbital period of 41.03236 +/- 0.00002 days, and component masses M_1 = 0.395 +/- 0.002 Msol and M_2 = 0.275 +/- 0.001 Msol in an eccentric (e = 0.239 +/- 0.002) orbit. A 65 day out of eclipse modulation of approximately 2% peak-to-peak amplitude is seen in I-band, which is probably due to rotational modulation of photospheric spots on one of the binary components. This paper presents the discovery and characterization of the object, including radial velocities sufficient to determine both component masses to better than 1% precision, and a photometric solution. We find that the sum of the component radii, which is much better-determined than the individual radii, is inflated by 3.8 +0.9 -0.5 % compared to the theoretical model predictions, depending on the age and metallicity assumed. These results demonstrate that the difficulties in reproducing observed M-dwarf eclipsing binary radii with theoretical models are not confined to systems with very short orbital periods. This object promises to be a fruitful testing ground for the hypothesized link between inflated radii in M-dwarfs and activity.Comment: 23 pages, 11 figures, 12 tables, emulateapj format. Accepted for publication in Ap

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer.

Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year (Zhang et al., 2017). To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 h of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer.

Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year (Zhang et al., 2017). To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 h of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts