Production and characterization of β-glucosidase from Gongronella butleri by solid-state fermentation

Among the enzymes of the cellulolytic complex, β-glucosidases are noteworthy due to the possibility of their application in different industrial processes, such as production of biofuels, winemaking, and development of functional foods. This study aimed to evaluate the production and characterization of β-glucosidase from the filamentous fungus Gongronella butleri, recently isolated from Cerrado soil and cultivated in agro-industrial residue substrates. The highest production of β-glucosidase, about 215.4 U/g of dry substrate (or 21.5 U/mL), was obtained by cultivation of the microorganism on wheat bran with 55% of the initial moisture, for 96 h at 30°C. This β-glucosidase showed higher catalytic activity at pH 4.5, and a temperature of 65°C. The original enzymatic activity was recovered in a pH range of 3.0-7.5 after 24 h of incubation. The enzyme retained 80% of its catalytic activity when incubated for 1 h at 50°C. The enzyme was strongly inhibited by glucose, an effect that was completely reversed by increasing substrate concentration in the reaction mixture, which is typical for competitive inhibition. High catalytic activity was observed in solutions containing up to 20% ethanol, allowing the application of this enzyme in processes with high alcohol concentrations (for example beverages and biofuels). The significant production of β-glucosidase by the selected strain, along with these enzyme characteristics, highlights the biotechnological potential of the fungus G. butleri.Key words: Microbial enzyme, biofuels, agro-industrial residues, cellulases, hemicellulases

Cenário E Perspectiva Da Produção Científica Sobre Competência Em Informação (coinfo) No Brasil: Estudo Da Produção No âmbito Da Ancib

Search highlight in the field of Information Science in Brazil, the importance of the publications generated by scientific events, specifically the scope of information literacy (Competência em Informação - CoInfo, in Portuguese), analyzing the work and the production of its main scientific society, the Associação Nacional de Pesquisa e Pós-Graduação em Ciência da Informação - ANCIB. Presents the results of research that involved the document corpus published in the editions of the Encontro Nacional de Pesquisa e Pós-Graduação em Ciência da Informação - Enancib, annual scientific agenda ANCIB, watching literature produced by scientific work on CoInfo presented in all Anais of the Enancib in the period 1994-2015, identifying theme insertion in all of its major working groups. The research used exploratory and descriptive approach, involving documentary and historical research. The search was carried out in the event portal ANCIB-Enancib, from the selection of the works related to the theme CoInfo and seven related terms: information literacy, competência informacional, alfabetização informacional, aprendizagem informacional, alfabetização em informação, letramento informacional, user education. For measurement data we used the Bardin technique. The results show that as of 2009 there is an increase in the production of work on CoInfo presented in Enancib, indicating that the spaces commonly used for the application of methodologies are libraries. These results also show that the document corpus dealing with the subject and the growing interest of researchers for it, coupled with the possibilities of seminars involving this theme in focus in recent programming Post-Enancib's, provides a very promising future vision for the studies, research and the effective consolidation of CoInfo in the field of Information Science in Brazil, and its visibility. In conclusion, presents the efforts made by various institutions for the development of CoInfo in Brazil in international impact event.26315116

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02