Spatial and Temporal Distributions of Ionospheric Irregularities Derived from Regional and Global ROTI Maps

Major advancements in the monitoring of both the occurrence and impacts of space weather can be made by evaluating the occurrence and distribution of ionospheric disturbances. Previous studies have shown that the fluctuations in total electron content (TEC) values estimated from Global Navigation Satellite System (GNSS) observations clearly exhibit the intensity levels of ionospheric irregularities, which vary continuously in both time and space. The duration and intensity of perturbations depend on the geographic location. They are also dependent on the physical activities of the Sun, the Earth’s magnetic activities, as well as the process of transferring energy from the Sun to the Earth. The aim of this study is to establish ionospheric irregularity maps using ROTI (rate of TEC index) values derived from conventional dual-frequency GNSS measurements (30-s interval). The research areas are located in Southeast Asia (15°S–25°N latitude and 95°E–115°E longitude), which is heavily affected by ionospheric scintillations, as well as in other regions around the globe. The regional ROTI map of Southeast Asia clearly indicates that ionospheric disturbances in this region are dominantly concentrated around the two equatorial ionization anomaly (EIA) crests, occurring mainly during the evening hours. Meanwhile, the global ROTI maps reveal the spatial and temporal distributions of ionospheric scintillations. Within the equatorial region, South America is the most vulnerable area (22.6% of total irregularities), followed by West Africa (8.2%), Southeast Asia (4.7%), East Africa (4.1%), the Pacific (3.8%), and South Asia (2.3%). The generated maps show that the scintillation occurrence is low in the mid-latitude areas during the last solar cycle. In the polar regions, ionospheric irregularities occur at any time of the day. To compare ionospheric disturbances between regions, the Earth is divided into ten sectors and their irregularity coefficients are calculated accordingly. The quantification of the degrees of disturbance reveals that about 58 times more ionospheric irregularities are observed in South America than in the southern mid-latitudes (least affected region). The irregularity coefficients in order from largest to smallest are as follows: South America, 3.49; the Arctic, 1.94; West Africa, 1.77; Southeast Asia, 1.27; South Asia, 1.24; the Antarctic, 1.10; East Africa, 0.89; the Pacific, 0.32; northern mid-latitudes, 0.15; southern mid-latitudes, 0.06

Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking

International audienc

Characterization of Conserved Tandem Donor Sites and Intronic Motifs Required for Alternative Splicing in Corticosteroid Receptor Genes

Alternative splicing events from tandem donor sites result in mRNA variants coding for additional amino acids in the DNA binding domain of both the glucocorticoid (GR) and mineralocorticoid (MR) receptors. We now show that expression of both splice variants is extensively conserved in mammalian species, providing strong evidence for their functional significance. An exception to the conservation of the MR tandem splice site (an A at position +5 of the MR+12 donor site in the mouse) was predicted to decrease U1 small nuclear RNA binding. In accord with this prediction, we were unable to detect the MR+12 variant in this species. The one exception to the conservation of the GR tandem splice site, an A at position +3 of the platypus GRγ donor site that was predicted to enhance binding of U1 snRNA, was unexpectedly associated with decreased expression of the variant from the endogenous gene as well as a minigene. An intronic pyrimidine motif present in both GR and MR genes was found to be critical for usage of the downstream donor site, and overexpression of TIA1/TIAL1 RNA binding proteins, which are known to bind such motifs, led to a marked increase in the proportion of GRγ and MR+12. These results provide striking evidence for conservation of a complex splicing mechanism that involves processes other than stochastic spliceosome binding and identify a mechanism that would allow regulation of variant expression

Plasmodium falciparum

Exit from the endoplasmic reticulum (ER) often occurs at distinct sites of vesicle formation known as transitional ER (tER) that are enriched for COPII vesicle coat proteins. We have characterized the organization of ER export in the malaria parasite, Plasmodium falciparum, by examining the localization of two components of the COPII machinery, PfSec12 and PfSec24a. PfSec12 was found throughout the ER, whereas the COPII cargo adaptor, PfSec24a, was concentrated at distinct foci that likely correspond to tER sites. These foci were closely apposed to cis-Golgi sites marked by PfGRASP-GFP, and upon treatment with brefeldin A they accumulated a model cargo protein via a process dependent on the presence of an intact diacidic export motif. Our data suggest that the cargo-binding function of PfSec24a is conserved and that accumulation of cargo in discrete tER sites depends upon positive sorting signals. Furthermore, the number and position of tER sites with respect to the cis-Golgi suggests a co-ordinated biogenesis of these domains