Furfural, 5-HMF, acid-soluble lignin and sugar contents in C. ladanifer and E. arborea lignocellulosic biomass hydrolysates obtained from microwave-assisted treatments in different solvents

Cistus ladanifer L. and Erica arborea L. are the two most representative shrub species from the Iberian Peninsula. With a view to their valorization, their biomass hydrolysate components, obtained from microwave-assisted treatments with choline chloride/urea - HNO3 10%, N,N-dimethylacetamide/NaHCO3 and N,N-dimethylacetamide/CH3OK as solvents, have been measured using a spectrophotometric method. Concentrations of furfural and 5-(hydroxymethyl)furfural (5-HMF) in the filtrate have been determined after reduction with NaBH4. The production of total sugars, reducing sugars and non-reducing sugars has also been assessed. The obtained results support the choice of microwave-assisted choline chloride/urea deep eutectic solvent in acid media as the preferred method (over the polar aprotic solvent-based alternatives) for the extraction of lignin, furfural, 5-HMF and sugars from C. ladanifer and E. arborea biomass, attaining the best production yields for 60¿min exposure times. Another is the case if the aim of the treatments is to recovery sugars from both shrubs for subsequent enzymatic saccharification: the very low 5-HMF contents resulting from the dimetylacetamide systems (especially is association with CH3OK) make them highly advantageous as compared to the traditional method using NaOH

Crystallinity of cellulose microfibers derived from Cistus ladanifer and Erica arborea shrubs

The effectiveness of the use of cellulose fibers as particulates/composite reinforcers involves the assessment of the crystallinity of such fibers. The aim of the present work is to provide information on the degree of crystallinity of the cellulose microfibers obtained from the stems of Cistus ladanifer and Erica arborea shrubs through two different methods, namely an alkaline treatment and a microwave-assisted deep eutectic solvent (DES) method. The crystallinity indexes (CrI) obtained from X-ray powder diffraction patterns indicated that higher CrI were attained for cellulose obtained from the DES treatment. Complementary information on the degree of crystallinity was also retrieved from attenuated total reflection- Fourier transform infrared spectroscopy (ATR-FTIR) vibrational spectra, scanning electron microscopy (SEM) micrographs, and accessibility data for the DES-treated celluloses from the two species. The crystallinity results for the fibers derived from these two Mediterranean shrubs were within the range of the results for those derived from wood pulp, opening the door to their valorization for cellulose-derived packing applications or for their use as reinforcers in composite materials in combination with other biopolymers

Speleoseismology and palaeoseismicity of Benis Cave (Murcia, SE Spain): coseismic effects of the 1999 Mula earthquake (mb 4.8)

This work describes the coseismic ceiling block collapse within Benis Cave (−213 m; Murcia, SE Spain), associated with the 1999 Mula earthquake (mb=4.8, MSK VII). The collapse occurred at −156 m into the Earthquake Hall, and as a consequence one small gallery became blind. We studied the geology, topography and active tectonic structures relevant to the cave. In addition, we carried out a seismotectonic analysis of the focal mechanism solutions, and also a fault population analysis on slickensides measured in fault planes in the cave. The stress and strain regime is interpreted as being congruent with the palaeoseismic evidence, and agrees with the fault kinematics established for cave galleries developed within fault planes and growth anomalies of coral flowstone. Our analysis suggests that one active segment (NNE–SSW) determined the morphology and topography of the Benis Cave, where strong to moderate palaeoearthquakes (6≤M≤7) took place. As a consequence of this intense seismic activity a small gallery collapsed. A new palaeoseismic structure, or seismothem, has been recognized, namely the effect of palaeoearthquakes affecting the pattern of development of the spatial coral flowstone distribution located at the bottom of the cave

Sugar beet agronomic performance evolution in NW Spain in future scenarios of climate change

Changes in environmental conditions resulting from Climate Change are expected to have a major impact on crops. In order to foresee adaptation measures and to minimize yield decline, it is necessary to estimate the effect of those changes on the evapotranspiration and on the associated irrigation needs of crops. In the study presented herein, future conditions extracted from RCP4.5 scenario of IPCC, particularized for Castilla-y-León (Spain), were used as inputs for FAO crop simulation model (AquaCrop) to estimate sugar beet agronomic performance in the medium-term (2050 and 2070). A regional analysis of future trends in terms of yield, biomass and CO2 sequestration was carried out. An annual ET0 increase of up to 200 mm was estimated in 2050 and 2070 scenarios, with ETc increases of up to 40 mm/month. At current irrigation levels, temperature rise would be accompanied by a 9% decrease in yield and a ca. 6% decrease in assimilated CO2 in the 2050 and 2070 scenarios. However, it is also shown that the implementation of adequate adaptation measures, in combination with a more efficient irrigation management, may result in up to 17% higher yields and in the storage of between 9% and 13% higher amounts of CO2

Valorization of Cistus ladanifer and Erica arborea shrubs for fuel: Wood and bark thermal characterization

As a form of upgraded biomass characterized by its high energy density, low production costs, and low process energy requirements, wood pellets are an environmentally friendly fuel allowing for carbon neutral heating with high energy efficiency. In this work, the suitability of a valorization of the woods from the two most representative shrub species from the Iberian Peninsula (namely Cistus ladanifer and Erica arborea) for heating has been assessed. Whereas Erica arborea met the requirements of ISO 17225-2:2014 for ENplus-B class (the calorific content for both wood and bark was high and not significantly different, and the ash content was permissible for specimens with branch diameter ≥2,8 cm), Cistus ladanifer was in the limit of the normative and only met the requirements in terms of acceptable ash percentage (1,9%) and heating value (19 kJ·g-1) for old specimens with branch diameters >3,4 cm. Consequently, while the harvest of E. arborea for its use as fuel does not need to be selective, that of C. ladanifer should be limited to the most robust specimens and foliage should be avoided.   PDF XM

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Familial hypercholesterolaemia in children and adolescents from 48 countries: a cross-sectional study

Background: Approximately 450 000 children are born with familial hypercholesterolaemia worldwide every year, yet only 2·1% of adults with familial hypercholesterolaemia were diagnosed before age 18 years via current diagnostic approaches, which are derived from observations in adults. We aimed to characterise children and adolescents with heterozygous familial hypercholesterolaemia (HeFH) and understand current approaches to the identification and management of familial hypercholesterolaemia to inform future public health strategies. Methods: For this cross-sectional study, we assessed children and adolescents younger than 18 years with a clinical or genetic diagnosis of HeFH at the time of entry into the Familial Hypercholesterolaemia Studies Collaboration (FHSC) registry between Oct 1, 2015, and Jan 31, 2021. Data in the registry were collected from 55 regional or national registries in 48 countries. Diagnoses relying on self-reported history of familial hypercholesterolaemia and suspected secondary hypercholesterolaemia were excluded from the registry; people with untreated LDL cholesterol (LDL-C) of at least 13·0 mmol/L were excluded from this study. Data were assessed overall and by WHO region, World Bank country income status, age, diagnostic criteria, and index-case status. The main outcome of this study was to assess current identification and management of children and adolescents with familial hypercholesterolaemia. Findings: Of 63 093 individuals in the FHSC registry, 11 848 (18·8%) were children or adolescents younger than 18 years with HeFH and were included in this study; 5756 (50·2%) of 11 476 included individuals were female and 5720 (49·8%) were male. Sex data were missing for 372 (3·1%) of 11 848 individuals. Median age at registry entry was 9·6 years (IQR 5·8-13·2). 10 099 (89·9%) of 11 235 included individuals had a final genetically confirmed diagnosis of familial hypercholesterolaemia and 1136 (10·1%) had a clinical diagnosis. Genetically confirmed diagnosis data or clinical diagnosis data were missing for 613 (5·2%) of 11 848 individuals. Genetic diagnosis was more common in children and adolescents from high-income countries (9427 [92·4%] of 10 202) than in children and adolescents from non-high-income countries (199 [48·0%] of 415). 3414 (31·6%) of 10 804 children or adolescents were index cases. Familial-hypercholesterolaemia-related physical signs, cardiovascular risk factors, and cardiovascular disease were uncommon, but were more common in non-high-income countries. 7557 (72·4%) of 10 428 included children or adolescents were not taking lipid-lowering medication (LLM) and had a median LDL-C of 5·00 mmol/L (IQR 4·05-6·08). Compared with genetic diagnosis, the use of unadapted clinical criteria intended for use in adults and reliant on more extreme phenotypes could result in 50-75% of children and adolescents with familial hypercholesterolaemia not being identified. Interpretation: Clinical characteristics observed in adults with familial hypercholesterolaemia are uncommon in children and adolescents with familial hypercholesterolaemia, hence detection in this age group relies on measurement of LDL-C and genetic confirmation. Where genetic testing is unavailable, increased availability and use of LDL-C measurements in the first few years of life could help reduce the current gap between prevalence and detection, enabling increased use of combination LLM to reach recommended LDL-C targets early in life

TRY plant trait database – enhanced coverage and open access

Plant traits - the morphological, anatomical, physiological, biochemical and phenological characteristics of plants - determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait‐based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits - almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Juxtaposing BTE and ATE – on the role of the European insurance industry in funding civil litigation

One of the ways in which legal services are financed, and indeed shaped, is through private insurance arrangement. Two contrasting types of legal expenses insurance contracts (LEI) seem to dominate in Europe: before the event (BTE) and after the event (ATE) legal expenses insurance. Notwithstanding institutional differences between different legal systems, BTE and ATE insurance arrangements may be instrumental if government policy is geared towards strengthening a market-oriented system of financing access to justice for individuals and business. At the same time, emphasizing the role of a private industry as a keeper of the gates to justice raises issues of accountability and transparency, not readily reconcilable with demands of competition. Moreover, multiple actors (clients, lawyers, courts, insurers) are involved, causing behavioural dynamics which are not easily predicted or influenced. Against this background, this paper looks into BTE and ATE arrangements by analysing the particularities of BTE and ATE arrangements currently available in some European jurisdictions and by painting a picture of their respective markets and legal contexts. This allows for some reflection on the performance of BTE and ATE providers as both financiers and keepers. Two issues emerge from the analysis that are worthy of some further reflection. Firstly, there is the problematic long-term sustainability of some ATE products. Secondly, the challenges faced by policymakers that would like to nudge consumers into voluntarily taking out BTE LEI

Search for stop and higgsino production using diphoton Higgs boson decays

Results are presented of a search for a "natural" supersymmetry scenario with gauge mediated symmetry breaking. It is assumed that only the supersymmetric partners of the top-quark (stop) and the Higgs boson (higgsino) are accessible. Events are examined in which there are two photons forming a Higgs boson candidate, and at least two b-quark jets. In 19.7 inverse femtobarns of proton-proton collision data at sqrt(s) = 8 TeV, recorded in the CMS experiment, no evidence of a signal is found and lower limits at the 95% confidence level are set, excluding the stop mass below 360 to 410 GeV, depending on the higgsino mass