Statistical relationship between date of sowing and the sorghum shootfly (Atherigona Soccata, Rondani L)

The present study was based on the available data of eleven years for shoot fly from 2000-2010 for kharif season. Different models viz., linear and non linear were tried to fit, Amongst, the linear, quadratic and cubic models produced better coefficient of determination and the models viz., EGG(Shoot fly Egg) =3.760+0.196(DOS) (R2 =0.892) and EGG(Shoot fly Egg) =1.077+1.195(DOS)-0.087(DOS^2), which produced highest R2 (0.896 at p=0.05) with less standard error (0.419) and quadratic model was also the best fit model in determining the oviposition of shoot fly, which explained 89.6 per cent variation in the oviposition of shoot fly for the 7 days after emergence of the sorghum crop. For the dead heart development (14 DAE), the model %DH (% Dead Heart) =3.535+3.104(DOS) found best fit with highest coefficient of determination of 0. 856 and exhibited significant positive relationship with the date of sowing and during 21 DAE the cubic model %DH (% Dead Heart) =10.619+10.115(DOS)-3.466(DOS^2)+0 .321(DOS ^3) had significant coefficient of determination value of 0.988 with least standard error 0.885. The quadratic model during the 28 days after emergence of the crop %DH (% Dead Heart) =-6.234+22.858(DOS) -1.399 (DOS^2) found best fit and produced significant R2 (0.929 at 5 per cent level) and showed better relationship with the date of sowing. It was found that, both linear and non linear relationship exists between dates of sowing and shoot fly of sorghum during kharif season

Global Vascular Guidelines on the Management of Chronic Limb-Threatening Ischemia

Chronic limb-threatening ischemia (CLTI)is associated with mortality, amputation, and impaired quality of life. These Global Vascular Guidelines (GVG)are focused on definition, evaluation, and management of CLTI with the goals of improving evidence-based care and highlighting critical research needs. The term CLTI is preferred over critical limb ischemia, as the latter implies threshold values of impaired perfusion rather than a continuum. CLTI is a clinical syndrome defined by the presence of peripheral artery disease (PAD)in combination with rest pain, gangrene, or a lower limb ulceration >2 weeks duration. Venous, traumatic, embolic, and nonatherosclerotic etiologies are excluded. All patients with suspected CLTI should be referred urgently to a vascular specialist. Accurately staging the severity of limb threat is fundamental, and the Society for Vascular Surgery Threatened Limb Classification system, based on grading of Wounds, Ischemia, and foot Infection (WIfI)is endorsed. Objective hemodynamic testing, including toe pressures as the preferred measure, is required to assess CLTI. Evidence-based revascularization (EBR)hinges on three independent axes: Patient risk, Limb severity, and ANatomic complexity (PLAN). Average-risk and high-risk patients are defined by estimated procedural and 2-year all-cause mortality. The GVG proposes a new Global Anatomic Staging System (GLASS), which involves defining a preferred target artery path (TAP)and then estimating limb-based patency (LBP), resulting in three stages of complexity for intervention. The optimal revascularization strategy is also influenced by the availability of autogenous vein for open bypass surgery. Recommendations for EBR are based on best available data, pending level 1 evidence from ongoing trials. Vein bypass may be preferred for average-risk patients with advanced limb threat and high complexity disease, while those with less complex anatomy, intermediate severity limb threat, or high patient risk may be favored for endovascular intervention. All patients with CLTI should be afforded best medical therapy including the use of antithrombotic, lipid-lowering, antihypertensive, and glycemic control agents, as well as counseling on smoking cessation, diet, exercise, and preventive foot care. Following EBR, long-term limb surveillance is advised. The effectiveness of nonrevascularization therapies (eg, spinal stimulation, pneumatic compression, prostanoids, and hyperbaric oxygen)has not been established. Regenerative medicine approaches (eg, cell, gene therapies)for CLTI should be restricted to rigorously conducted randomizsed clinical trials. The GVG promotes standardization of study designs and end points for clinical trials in CLTI. The importance of multidisciplinary teams and centers of excellence for amputation prevention is stressed as a key health system initiative. © 2019 Society for Vascular Surgery and European Society for Vascular Surger

ERIC-PCR based genetic variability and their relationship with pathogenic variability of <em>Xanthomonas campestris</em> pv. <em>Campestris</em> isolates of West Bengal, India

620-626Black rot of cabbage caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most devastating diseases of cabbage causing 10-50% loss consistently. For determining genetic diversity amplification of enterobacterial repetitive intergenic consensus (ERIC-PCR) was reported to be reliable rapid and highly discriminatory. Therefore, current study was undertaken to determine genetic variability of 31 Xcc and five other xanthomonads collected from West Bengal using ERIC-PCR. Based on virulence on cabbage isolate 1.3W and 2.2 were found to be most virulent whereas isolates ND,1,2,6, and 3.1 were least virulent. Based on the ERIC-PCR fingerprints Xcc isolates were clearly distinguish from other xanthomonads. Several bands were identified unique to Xcc or other xanthomonads which can be used as markers for detection at the species and pathovar level. The grouping of the isolates based on ERIC-PCR was in accordance with the virulence of the isolates on cabbage. Genomic fingerprinting with ERIC-PCR revealed groups that did not represent significant relationship between isolates and geographical location isolates and host. However, it did demonstrate existence of genetic differences within the West Bengal X. campestris pv. Campestris population indicating that it is not a similar clonal population of the same genetic background