Formation and subdivision of the head field in the centipede Strigamia maritima, as revealed by the expression of head gap gene orthologues and hedgehog dynamics.

BACKGROUND: There have been few studies of head patterning in non-insect arthropods, and even in the insects, much is not yet understood. In the fly Drosophila three head gap genes, orthodenticle (otd), buttonhead (btd) and empty spiracles (ems) are essential for patterning the head. However, they do not act through the same pair-rule genes that pattern the trunk from the mandibular segment backwards. Instead they act through the downstream factors collier (col) and cap'n'collar (cnc), and presumably other unknown factors. In the beetle Tribolium, these same gap and downstream genes are also expressed during early head development, but in more restricted domains, and some of them have been shown to be of minor functional importance. In the spider Parasteatoda tepidariorum, hedgehog (hh) and otd have been shown to play an important role in head segmentation. RESULTS: We have investigated the expression dynamics of otx (otd), SP5/btd, ems, and the downstream factors col, cnc and hh during early head development of the centipede Strigamia maritima. Our results reveal the process of head condensation and show that the anteroposterior sequence of specific gene expression is conserved with that in insects. SP5/btd and otx genes are expressed prior to and during head field formation, whereas ems is not expressed until after the initial formation of the head field, in an emerging gap between SP5/btd and otx expression. Furthermore, we observe an early domain of Strigamia hh expression in the head field that splits to produce segmental stripes in the ocular, antennal and intercalary segments. CONCLUSIONS: The dynamics of early gene expression in the centipede show considerable similarity with that in the beetle, both showing more localised expression of head gap genes than occurs in the fly. This suggests that the broad overlapping domains of head gap genes observed in Drosophila are derived in this lineage. We also suggest that the splitting of the early hh segmental stripes may reflect an ancestral and conserved process in arthropod head patterning. A remarkably similar stripe splitting process has been described in a spider, and in the Drosophila head hh expression starts from a broad domain that transforms into three stripes

Entwicklung weit frequenzabstimmbarer einfrequenter Laserstrahlquellen für Raumfahrtanwendungen

[no abstract

Literary transmission, exile, and oblivion: Gustav von Schlabrendorf meets Henry Crabb Robinson

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Imagination and Growth: Coleridge and Wordsworth in Germany (1798-99)

On 16 September 1798 the packet boat with Dorothy and William Wordsworth, Samuel Taylor Coleridge, and his Nether Stowey friend John Chester on board sailed from Yarmouth to arrive in Hamburg three days later (Frank 220). Behind Coleridge and Wordsworth lay the year of shared creativity that Wordsworth refers to in the lines quoted above (Owen 270), and that culminated in the first edition of the Lyrical Ballads, published in Britain on 4 October 1798 (Gill Oxford DNB), a mere two weeks after its authors had disembarked in the German Hanseatic city. Before Coleridge and Wordsworth lay a long, fiercely cold winter of separation; the Wordsworths spent it in Goslar, a decaying medieval town in Lower Saxony, whereas Coleridge and Chester first stayed in Ratzeburg and then, in February 1799, moved on to the then thriving university town of Göttingen. While Coleridge was learning German and coming into close contact with German academia, the Wordsworths lived a secluded life in Goslar. Here, Wordsworth sought to compose The Recluse, his intended poetical masterpiece which envisaged Coleridge as a contributor of thought (Wu 189; 448), and which may have taken their shared creativity to a new level. Nevertheless, Wordsworth found himself unable to prolong this joint creativity through writing The Recluse in the absence of Coleridge, in whose company he had spent “virtually every day” of the preceding year (Wu 189). Instead, Wordsworth began his lasting poetical venture The Prelude – and, in that same narrow space and timeframe – composed the majority of the “Lucy Poems” (300; 326; 356). These poems will be referred to in inverted commas, since Wordsworth never grouped them as such; Victorian scholars initiated the grouping that has led to the modern canon (Jones 7). This paper focuses on how the months in Germany – from September 1798 to late April 1799 in Wordsworth’s case, and to July of the same year in Coleridge’s – influenced the poets’ joint as well as individual creativity. The paper’s central claim is that Wordsworth invented the character of Lucy in order to voice his anxiety about the endangered mutual creativity in Coleridge’s absence, and that the “Lucy Poems,” just as The Prelude, address Coleridge. The “Lucy Poems” complement and extend The Prelude; they leave Wordsworth with the composition of The Prelude as his poetic collaboration with Coleridge comes to an abrupt halt, while the “Lucy Poems” also pick up the reader where The Prelude leaves them, namely at the point in Wordsworth’s poeticised autobiography where he is about to meet Coleridge, and where their collaboration is about to begin. In all her luminous imagery, Lucy is the poetic personification, the enlightening “Phantom” of Coleridge’s and Wordsworth’s shared creative imagination behind the Lyrical Ballads that the poets envisaged to grow into The Recluse; she is the “happiness” of “that summer” of 1798 in The Prelude’s “Book Fourteenth.

Synaptic and peptidergic connectome of a neurosecretory centre in the annelid brain

This is the author accepted manuscript. The final version is available from eLife Sciences Publications via the DOI in this record.Neurosecretory centers in animal brains use peptidergic signaling to influence physiology and behavior. Understanding neurosecretory center function requires mapping cell types, synapses, and peptidergic networks. Here we use transmission electron microscopy and gene expression mapping to analyze the synaptic and peptidergic connectome of an entire neurosecretory center. We reconstructed 78 neurosecretory neurons and mapped their synaptic connectivity in the brain of larval Platynereis dumerilii, a marine annelid. These neurons form an anterior neurosecretory center expressing many neuropeptides, including hypothalamic peptide orthologs and their receptors. Analysis of peptide-receptor pairs in spatially mapped single-cell transcriptome data revealed sparsely connected networks linking specific neuronal subsets. We experimentally analyzed one peptide-receptor pair and found that a neuropeptide can couple neurosecretory and synaptic brain signaling. Our study uncovered extensive networks of peptidergic signaling within a neurosecretory center and its connection to the synaptic brain.The research leading to these results received funding from the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013)/ European Research Council Grant Agreement 260821. The research was supported by a grant from the DFG - Deutsche Forschungsgemeinschaft (Reference no. JE 777/1)

The first myriapod genome sequence reveals conservative arthropod gene content and genome organisation in the centipede Strigamia maritima.

Myriapods (e.g., centipedes and millipedes) display a simple homonomous body plan relative to other arthropods. All members of the class are terrestrial, but they attained terrestriality independently of insects. Myriapoda is the only arthropod class not represented by a sequenced genome. We present an analysis of the genome of the centipede Strigamia maritima. It retains a compact genome that has undergone less gene loss and shuffling than previously sequenced arthropods, and many orthologues of genes conserved from the bilaterian ancestor that have been lost in insects. Our analysis locates many genes in conserved macro-synteny contexts, and many small-scale examples of gene clustering. We describe several examples where S. maritima shows different solutions from insects to similar problems. The insect olfactory receptor gene family is absent from S. maritima, and olfaction in air is likely effected by expansion of other receptor gene families. For some genes S. maritima has evolved paralogues to generate coding sequence diversity, where insects use alternate splicing. This is most striking for the Dscam gene, which in Drosophila generates more than 100,000 alternate splice forms, but in S. maritima is encoded by over 100 paralogues. We see an intriguing linkage between the absence of any known photosensory proteins in a blind organism and the additional absence of canonical circadian clock genes. The phylogenetic position of myriapods allows us to identify where in arthropod phylogeny several particular molecular mechanisms and traits emerged. For example, we conclude that juvenile hormone signalling evolved with the emergence of the exoskeleton in the arthropods and that RR-1 containing cuticle proteins evolved in the lineage leading to Mandibulata. We also identify when various gene expansions and losses occurred. The genome of S. maritima offers us a unique glimpse into the ancestral arthropod genome, while also displaying many adaptations to its specific life history.This work was supported by the following grants: NHGRIU54HG003273 to R.A.G; EU Marie Curie ITN #215781 “Evonet” to M.A.; a Wellcome Trust Value in People (VIP) award to C.B. and Wellcome Trust graduate studentship WT089615MA to J.E.G; Marine rhythms of Life” of the University of Vienna, an FWF (http://www.fwf.ac.at/) START award (#AY0041321) and HFSP (http://www.hfsp.org/) research grant (#RGY0082/2010) to KT-­‐R; MFPL Vienna International PostDoctoral Program for Molecular Life Sciences (funded by Austrian Ministry of Science and Research and City of Vienna, Cultural Department -­‐Science and Research to T.K; Direct Grant (4053034) of the Chinese University of Hong Kong to J.H.L.H.; NHGRI HG004164 to G.M.; Danish Research Agency (FNU), Carlsberg Foundation, and Lundbeck Foundation to C.J.P.G.; U.S. National Institutes of Health R01AI55624 to J.H.W.; Royal Society University Research fellowship to F.M.J.; P.D.E. was supported by the BBSRC via the Babraham Institute;This is the final version of the article. It first appeared from PLOS via http://dx.doi.org/10.1371/journal.pbio.100200

The mysterious green streaks below STEVE

Strong thermal emission velocity enhancement (STEVE) is an optical phenomenon of the subauroral ionosphere arising from extreme ion drift speeds. STEVE consists of two distinct components in true‐color imagery: a mauve or whitish arc extended in the magnetic east–west direction and a region of green emission adjacent to the arc, often structured into quasiperiodic columns aligned with the geomagnetic field (the “picket fence”). This work employs high‐resolution imagery by citizen scientists in a critical examination of fine‐scale features within the green emission region. Of particular interest are narrow “streaks” of emission forming underneath field‐aligned picket fence elements in the 100‐ to 110‐km altitude range. The streaks propagate in curved trajectories with dominant direction toward STEVE from the poleward side. The elongation is along the direction of motion, suggesting a drifting point‐like excitation source, with the apparent elongation due to a combination of motion blur and radiative lifetime effects. The cross‐sectional dimension is <1 km, and the cases observed have a duration of ∼20–30 s. The uniform coloration of all STEVE green features in these events suggests a common optical spectrum dominated by the oxygen 557.7‐nm emission line. The source is most likely direct excitation of ambient oxygen by superthermal electrons generated by ionospheric turbulence induced by the extreme electric fields driving STEVE. Some conjectures about causal connections with overlying field‐aligned structures are presented, based on coupling of thermal and gradient‐drift instabilities, with analogues to similar dynamics observed from chemical release and ionospheric heating experiments.https://www.essoar.org/doi/pdf/10.1002/essoar.10502878.1First author draf

A developmental perspective on the evolution of the nervous system.

The evolution of nervous systems in animals has always fascinated biologists, and thus multiple evolutionary scenarios have been proposed to explain the appearance of neurons and complex neuronal centers. However, the absence of a robust phylogenetic framework for animal interrelationships, the lack of a mechanistic understanding of development, and a recapitulative view of animal ontogeny have traditionally limited these scenarios. Only recently, the integration of advanced molecular and morphological studies in a broad range of animals has allowed to trace the evolution of developmental and neuronal characters on a better-resolved animal phylogeny. This has falsified most traditional scenarios for nervous system evolution, paving the way for the emergence of new testable hypotheses. Here we summarize recent progress in studies of nervous system development in major animal lineages and formulate some of the arising questions. In particular, we focus on how lineage analyses of nervous system development and a comparative study of the expression of neural-related genes has influenced our understanding of the evolution of an elaborated central nervous system in Bilateria. We argue that a phylogeny-guided study of neural development combining thorough descriptive and functional analyses is key to establish more robust scenarios for the origin and evolution of animal nervous systems