81 research outputs found
Implementation considerations in supervisory control
With supervisory control theory it is possible to describe controllers which influence the behaviour of a system by disabling controllable events. But sometimes it is desirable to have a controller which not only disables controllable events but also chooses one among the enabled ones. This event can be interpreted as a command given to the plant. This idea is formalized in the concept of an implementation, which is a special supervisor, enabling at most one controllable event at a time. In this paper, some useful properties are introduced, which ensure, when met, that each implementation of a given DES is nonblocking. The approach is applied to a simple chemical batch process example
Identification of histone modifications in biomedical text for supporting epigenomic research
Corinna K, Klinger R, Hofmann-Apitius M. Identification of Histone Modifications in Biomedical Text for Supporting Epigenomic Research. BMC Bioinformatics. 2009;10(Suppl 1):S28
Unusual multisystemic involvement and a novel BAG3 mutation revealed by NGS screening in a large cohort of myofibrillar myopathies
Background: Myofibrillar myopathies (MFM) are a group of phenotypically and genetically heterogeneous neuromuscular disorders, which are characterized by protein aggregations in muscle fibres and can be associated with multisystemic involvement. Methods: We screened a large cohort of 38 index patients with MFM for mutations in the nine thus far known causative genes using Sanger and next generation sequencing (NGS). We studied the clinical and histopathological characteristics in 38 index patients and five additional relatives (nâ=â43) and particularly focused on the associated multisystemic symptoms. Results: We identified 14 heterozygous mutations (diagnostic yield of 37%), among them the novel p.Pro209Gln mutation in the BAG3 gene, which was associated with onset in adulthood, a mild phenotype and an axonal sensorimotor polyneuropathy, in the absence of giant axons at the nerve biopsy. We revealed several novel clinical phenotypes and unusual multisystemic presentations with previously described mutations: hearing impairment with a FLNC mutation, dysphonia with a mutation in DES and the first patient with a FLNC mutation presenting respiratory insufficiency as the initial symptom. Moreover, we described for the first time respiratory insufficiency occurring in a patient with the p.Gly154Ser mutation in CRYAB. Interestingly, we detected a polyneuropathy in 28% of the MFM patients, including a BAG3 and a MYOT case, and hearing impairment in 13%, including one patient with a FLNC mutation and two with mutations in the DES gene. In four index patients with a mutation in one of the MFM genes, typical histological findings were only identified at the ultrastructural level (29%). Conclusions: We conclude that extraskeletal symptoms frequently occur in MFM, particularly cardiac and respiratory involvement, polyneuropathy and/or deafness. BAG3 mutations should be considered even in cases with a mild phenotype or an adult onset. We identified a genetic defect in one of the known genes in less than half of the MFM patients, indicating that more causative genes are still to be found. Next generation sequencing techniques should be helpful in achieving this aim
Prediction of Protein Binding Regions in Disordered Proteins
Many disordered proteins function via binding to a structured partner and undergo
a disorder-to-order transition. The coupled folding and binding can confer
several functional advantages such as the precise control of binding specificity
without increased affinity. Additionally, the inherent flexibility allows the
binding site to adopt various conformations and to bind to multiple partners.
These features explain the prevalence of such binding elements in signaling and
regulatory processes. In this work, we report ANCHOR, a method for the
prediction of disordered binding regions. ANCHOR relies on the pairwise energy
estimation approach that is the basis of IUPred, a previous general disorder
prediction method. In order to predict disordered binding regions, we seek to
identify segments that are in disordered regions, cannot form enough favorable
intrachain interactions to fold on their own, and are likely to gain stabilizing
energy by interacting with a globular protein partner. The performance of ANCHOR
was found to be largely independent from the amino acid composition and adopted
secondary structure. Longer binding sites generally were predicted to be
segmented, in agreement with available experimentally characterized examples.
Scanning several hundred proteomes showed that the occurrence of disordered
binding sites increased with the complexity of the organisms even compared to
disordered regions in general. Furthermore, the length distribution of binding
sites was different from disordered protein regions in general and was dominated
by shorter segments. These results underline the importance of disordered
proteins and protein segments in establishing new binding regions. Due to their
specific biophysical properties, disordered binding sites generally carry a
robust sequence signal, and this signal is efficiently captured by our method.
Through its generality, ANCHOR opens new ways to study the essential functional
sites of disordered proteins
Human amnion epithelial cells rescue cell death via immunomodulation of microglia in a mouse model of perinatal brain injury
Text mining and manual curation of chemical-gene-disease networks for the Comparative Toxicogenomics Database (CTD)
Measurement of the W±Z boson pair-production cross section in pp collisions at âs=13TeV with the ATLAS detector
published_or_final_versio
Charged-particle distributions at low transverse momentum in âs=13 13 TeV pp interactions measured with the ATLAS detector at the LHC
Measurements of distributions of charged particles produced in protonâproton collisions with a centre-of-mass energy of 13 TeV are presented. The data were recorded by the ATLAS detector at the LHC and correspond to an integrated luminosity of 151 ÎŒb â1 ÎŒbâ1 . The particles are required to have a transverse momentum greater than 100 MeV and an absolute pseudorapidity less than 2.5. The charged-particle multiplicity, its dependence on transverse momentum and pseudorapidity and the dependence of the mean transverse momentum on multiplicity are measured in events containing at least two charged particles satisfying the above kinematic criteria. The results are corrected for detector effects and compared to the predictions from several Monte Carlo event generators
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