DEGRADABILIDADE in vitro DA PROTEÍNA DE ESPÉCIES ARBÓREAS DO SEMI-ÁRIDO BRASILEIRO

This experiment was carried out to evaluate the protein degradability in vitro of arboreal plants from Brazilian semi-arid. The plants tested were angico (Anadenanthera colubrina), algaroba (Prosopis juliflora), espinheiro (Acacia glomerosa), juazeiro (Ziziphus joazeiro) and soybean meal, which had crude protein values of 22,5 16,0, 22,0, 19,0 and 38,0%, respectively. Samples (60 mg) were incubated with McDougal (8 ml) and inoculated ruminal fluid (2 ml). Aliquots (1 ml) were taking after 0, 6, 12, 24, 36 and 48 hours for analysis of ammonia, soluble protein and microbial protein. Soybean meal and the algaroba showed larger ammonia production and concentration of soluble protein compared to the other treatments. Soybean meal also displayed higher values for microbial protein. The treatment with angico was the one that displayed closed values towards soybean in regard to microbial protein, in spite of showing lesser ammonia production. The largest value of potential degradability 66,47%, was observed for the treatment with soybean meal, followed by the algaroba 46,27% and of the angico 45,20. Very low degradability was observed for the espinheiro and juazeiro, 19,16 e16,63%, respectively. Among the studied plants the angico and the algaroba were better sources of protein.O experimento teve como objetivo avaliar a degradabilidade in vitro da proteína de plantas arbóreas do semi-árido brasileiro e do farelo de soja. As plantas utilizadas foram angico (Anadenanthera colubrina), algaroba (Prosopis juliflora), espinheiro (Acacia glomerosa), juazeiro (Ziziphus joazeiro) e o farelo de soja, com valores de proteína bruta de 22,5 16,0, 22,0, 19,0 e 38,0%, respectivamente. Amostras de cada planta (60 mg) foram incubadas em 8 ml de meio tampão de McDougal, com adição de 2 ml de inóculo ruminal. Nos tempos 0, 6, 12, 24, 36 e 48 horas após a incubação foi retirada uma alíquota de 1,0 ml para análise de amônia, proteína solúvel e proteína microbiana. O farelo de soja e a algaroba proporcionaram maiores produção de amônia e concentração de proteína solúvel em relação aos outros alimentos, sendo que o farelo de soja apresentou maior valor de proteína microbiana. O tratamento com o angico foi o que mais se aproximou à soja com relação ao valor de proteína microbiana, apesar da sua menor produção de amônia. O maior valor de degradabilidade potencial 66,47 %, foi observado no tratamento com farelo de soja, seguidos dos tratamentos da algaroba 46,27% e angico 45,20%. Foram observados valores de degradabilidade muito baixos para o espinheiro e juazeiro, 19,16 e 16,63%, respectivamente. O angico e a algaroba apresentaram-se como melhores fontes de proteína entre as plantas avaliadas

Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy

The objectives of this study were to investigate the effect of laser-induced surface features on the morphology, attachment and viability of mesenchymal stem cells (MSCs) at different periods of time, and to evaluate the biocompatibility of different zones: laser-melted zone (MZ), heat-affected zone (HAZ) and base metal (BM) in laser-treated NiTi alloy. The surface morphology and composition were studied by scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS), respectively. The cell morphology was examined by SEM while the cell counting and viability measurements were done by hemocytometer and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The results indicated that the laser-induced surface features, such as surface roughening, presence of anisotropic dendritic pattern and complete surface Ni oxidation were beneficial to improve the biocompatibility of NiTi as evidenced by the highest cell attachment (4 days of culture) and viability (7 days of culture) found in the MZ. The biocompatibility of the MZ was the best, followed by the BM with the HAZ being the worst. The defective and porous oxide layer as well as the coarse grained structure might attribute to the inferior cell attachment (4 days of culture) and viability (7 days of culture) on the HAZ compared with the BM which has similar surface morphology. © 2014 Elsevier B.V

Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV

Results are presented from a search for a W' boson using a dataset corresponding to 5.0 inverse femtobarns of integrated luminosity collected during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV. The W' boson is modeled as a heavy W boson, but different scenarios for the couplings to fermions are considered, involving both left-handed and right-handed chiral projections of the fermions, as well as an arbitrary mixture of the two. The search is performed in the decay channel W' to t b, leading to a final state signature with a single lepton (e, mu), missing transverse energy, and jets, at least one of which is tagged as a b-jet. A W' boson that couples to fermions with the same coupling constant as the W, but to the right-handed rather than left-handed chiral projections, is excluded for masses below 1.85 TeV at the 95% confidence level. For the first time using LHC data, constraints on the W' gauge coupling for a set of left- and right-handed coupling combinations have been placed. These results represent a significant improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters

Measurement of the Lambda(b) cross section and the anti-Lambda(b) to Lambda(b) ratio with Lambda(b) to J/Psi Lambda decays in pp collisions at sqrt(s) = 7 TeV

The Lambda(b) differential production cross section and the cross section ratio anti-Lambda(b)/Lambda(b) are measured as functions of transverse momentum pt(Lambda(b)) and rapidity abs(y(Lambda(b))) in pp collisions at sqrt(s) = 7 TeV using data collected by the CMS experiment at the LHC. The measurements are based on Lambda(b) decays reconstructed in the exclusive final state J/Psi Lambda, with the subsequent decays J/Psi to an opposite-sign muon pair and Lambda to proton pion, using a data sample corresponding to an integrated luminosity of 1.9 inverse femtobarns. The product of the cross section times the branching ratio for Lambda(b) to J/Psi Lambda versus pt(Lambda(b)) falls faster than that of b mesons. The measured value of the cross section times the branching ratio for pt(Lambda(b)) > 10 GeV and abs(y(Lambda(b))) < 2.0 is 1.06 +/- 0.06 +/- 0.12 nb, and the integrated cross section ratio for anti-Lambda(b)/Lambda(b) is 1.02 +/- 0.07 +/- 0.09, where the uncertainties are statistical and systematic, respectively.Comment: Submitted to Physics Letters

Search for new physics in events with opposite-sign leptons, jets, and missing transverse energy in pp collisions at sqrt(s) = 7 TeV

A search is presented for physics beyond the standard model (BSM) in final states with a pair of opposite-sign isolated leptons accompanied by jets and missing transverse energy. The search uses LHC data recorded at a center-of-mass energy sqrt(s) = 7 TeV with the CMS detector, corresponding to an integrated luminosity of approximately 5 inverse femtobarns. Two complementary search strategies are employed. The first probes models with a specific dilepton production mechanism that leads to a characteristic kinematic edge in the dilepton mass distribution. The second strategy probes models of dilepton production with heavy, colored objects that decay to final states including invisible particles, leading to very large hadronic activity and missing transverse energy. No evidence for an event yield in excess of the standard model expectations is found. Upper limits on the BSM contributions to the signal regions are deduced from the results, which are used to exclude a region of the parameter space of the constrained minimal supersymmetric extension of the standard model. Additional information related to detector efficiencies and response is provided to allow testing specific models of BSM physics not considered in this paper.Comment: Replaced with published version. Added journal reference and DO

Measurement of isolated photon production in pp and PbPb collisions at sqrt(sNN) = 2.76 TeV

Isolated photon production is measured in proton-proton and lead-lead collisions at nucleon-nucleon centre-of-mass energies of 2.76 TeV in the pseudorapidity range |eta|<1.44 and transverse energies ET between 20 and 80 GeV with the CMS detector at the LHC. The measured ET spectra are found to be in good agreement with next-to-leading-order perturbative QCD predictions. The ratio of PbPb to pp isolated photon ET-differential yields, scaled by the number of incoherent nucleon-nucleon collisions, is consistent with unity for all PbPb reaction centralities.Comment: Submitted to Physics Letters