Development of bovine embryos cultured with media containing glucose.

Blastocyst (BL) expansion and blastocoel formation are coupled with increase in the glucose (GLU) metabolism, which is higher in morphologically normal than in degenerate embryos (Rieger et aI., 1992). This study aimed to determine the dose levei and duration of addition of GLU to culture medium that ensures the best results in development of in vitro produced bovine embryos

La investigación escolar en la construcción de explicaciones acerca de la transmisión de caracteres hereditarios. sistematización de la unidad didáctica ¡moscas!... a enseñar herencia

El presente trabajo expondrá el desarrollo significativo respecto a conceptos, procedimientos y actitudes referidos a la herencia de caracteres hereditarios, que alcanzaron los estudiantes del grado octavo del IEDIT Rodrigo de Triana, en un periodo de tres meses en el año 2008, durante el cual se ejecutó el diseño, aplicación y sistematización de la unidad didáctica ¡Moscas! a enseñar herencia. En esta experiencia educativa se adoptaron los postulados del Enfoque de Enseñanza por Investigación Dirigida y el instrumento metodológico de Investigación Acción, donde los estudiantes organizados en equipos de trabajo abordaron una pregunta problema para desarrollar una investigación escolar, bajo la atenta dirección de las docentes, quienes corroboraron un cambio procedimental, conceptual y actitudinal en los estudiantes del grado 803

La investigación escolar en la construcción de explicaciones acerca de la transmisión de caracteres hereditarios. sistematización de la unidad didáctica ¡moscas!... a enseñar herencia

El presente trabajo expondrá el desarrollo significativo respecto a conceptos, procedimientos y actitudes referidos a la herencia de caracteres hereditarios, que alcanzaron los estudiantes del grado octavo del IEDIT Rodrigo de Triana, en un periodo de tres meses en el año 2008, durante el cual se ejecutó el diseño, aplicación y sistematización de la unidad didáctica ¡Moscas! a enseñar herencia. En esta experiencia educativa se adoptaron los postulados del Enfoque de Enseñanza por Investigación Dirigida y el instrumento metodológico de Investigación Acción, donde los estudiantes organizados en equipos de trabajo abordaron una pregunta problema para desarrollar una investigación escolar, bajo la atenta dirección de las docentes, quienes corroboraron un cambio procedimental, conceptual y actitudinal en los estudiantes del grado 803

The Sloan Digital Sky Survey Reverberation Mapping Project : the MBH–host relations at 0.2 ∼< z ∼< 0.6 from reverberation mapping and Hubble Space Telescope imaging

Funding: Y.S. acknowledges support from an Alfred P. Sloan Research Fellowship and NSF grants AST-1715579, AST-2009947. Support for Program number HST-GO-14109 was provided through a grant from the STScI under NASA contract NAS5-26555. L.C.H. was supported by the National Key R&D Program of China (2016YFA0400702) and the National Science Foundation of China (11721303, 11991052). E.D.B. is supported by Padua University grants DOR1715817/17, DOR1885254/18, and DOR1935272/19 and by MIUR grant PRIN 201720173ML3WW_001. J.V.H.S.and K.H. acknowledge funds from a Science and Technology Facilities Council grant ST/R000824/1.We present the results of a pilot Hubble Space Telescope (HST) imaging study of the host galaxies of ten quasars from the Sloan Digital Sky Survey Reverberation Mapping (SDSS-RM) project. Probing more than an order of magnitude in BH and stellar masses, our sample is the first statistical sample to study the BH-host correlations beyond z>0.3 with reliable BH masses from reverberation mapping rather than from single-epoch spectroscopy. We perform image decomposition in two HST bands (UVIS-F606W and IR-F110W) to measure host colors and estimate stellar masses using empirical relations between broad-band colors and the mass-to-light ratio. The stellar masses of our targets are mostly dominated by a bulge component. The BH masses and stellar masses of our sample broadly follow the same correlations found for local RM AGN and quiescent bulge-dominant galaxies, with no strong evidence of evolution in the MBH-M*bulge relation to z~0.6. We further compare the host light fraction from HST imaging decomposition to that estimated from spectral decomposition. We found a good correlation between the host fractions derived with both methods. However, the host fraction derived from spectral decomposition is systematically smaller than that from imaging decomposition by ~30%, indicating different systematics in both approaches. This study paves the way for upcoming more ambitious host galaxy studies of quasars with direct RM-based BH masses at high redshift.PostprintPeer reviewe

Hemoglobin Uptake by Paracoccidioides spp. Is Receptor-Mediated

Iron is essential for the proliferation of fungal pathogens during infection. The availability of iron is limited due to its association with host proteins. Fungal pathogens have evolved different mechanisms to acquire iron from host; however, little is known regarding how Paracoccidioides species incorporate and metabolize this ion. In this work, host iron sources that are used by Paracoccidioides spp. were investigated. Robust fungal growth in the presence of the iron-containing molecules hemin and hemoglobin was observed. Paracoccidioides spp. present hemolytic activity and have the ability to internalize a protoporphyrin ring. Using real-time PCR and nanoUPLC-MSE proteomic approaches, fungal growth in the presence of hemoglobin was shown to result in the positive regulation of transcripts that encode putative hemoglobin receptors, in addition to the induction of proteins that are required for amino acid metabolism and vacuolar protein degradation. In fact, one hemoglobin receptor ortholog, Rbt5, was identified as a surface GPI-anchored protein that recognized hemin, protoporphyrin and hemoglobin in vitro. Antisense RNA technology and Agrobacterium tumefaciensmediated transformation were used to generate mitotically stable Pbrbt5 mutants. The knockdown strain had a lower survival inside macrophages and in mouse spleen when compared with the parental strain, which suggested that Rbt5 could act as a virulence factor. In summary, our data indicate that Paracoccidioides spp. can use hemoglobin as an iron source most likely through receptor-mediated pathways that might be relevant for pathogenic mechanisms

Measurement of the t t-bar production cross section in the dilepton channel in pp collisions at sqrt(s) = 7 TeV

The t t-bar production cross section (sigma[t t-bar]) is measured in proton-proton collisions at sqrt(s) = 7 TeV in data collected by the CMS experiment, corresponding to an integrated luminosity of 2.3 inverse femtobarns. The measurement is performed in events with two leptons (electrons or muons) in the final state, at least two jets identified as jets originating from b quarks, and the presence of an imbalance in transverse momentum. The measured value of sigma[t t-bar] for a top-quark mass of 172.5 GeV is 161.9 +/- 2.5 (stat.) +5.1/-5.0 (syst.) +/- 3.6(lumi.) pb, consistent with the prediction of the standard model.Comment: Replaced with published version. Included journal reference and DO

Search for the standard model Higgs boson in the H to ZZ to 2l 2nu channel in pp collisions at sqrt(s) = 7 TeV

A search for the standard model Higgs boson in the H to ZZ to 2l 2nu decay channel, where l = e or mu, in pp collisions at a center-of-mass energy of 7 TeV is presented. The data were collected at the LHC, with the CMS detector, and correspond to an integrated luminosity of 4.6 inverse femtobarns. No significant excess is observed above the background expectation, and upper limits are set on the Higgs boson production cross section. The presence of the standard model Higgs boson with a mass in the 270-440 GeV range is excluded at 95% confidence level.Comment: Submitted to JHE

Single hadron response measurement and calorimeter jet energy scale uncertainty with the ATLAS detector at the LHC

The uncertainty on the calorimeter energy response to jets of particles is derived for the ATLAS experiment at the Large Hadron Collider (LHC). First, the calorimeter response to single isolated charged hadrons is measured and compared to the Monte Carlo simulation using proton-proton collisions at centre-of-mass energies of sqrt(s) = 900 GeV and 7 TeV collected during 2009 and 2010. Then, using the decay of K_s and Lambda particles, the calorimeter response to specific types of particles (positively and negatively charged pions, protons, and anti-protons) is measured and compared to the Monte Carlo predictions. Finally, the jet energy scale uncertainty is determined by propagating the response uncertainty for single charged and neutral particles to jets. The response uncertainty is 2-5% for central isolated hadrons and 1-3% for the final calorimeter jet energy scale.Comment: 24 pages plus author list (36 pages total), 23 figures, 1 table, submitted to European Physical Journal