Second generation of viral vectors based on pepino mosaic virus

[SPA] Nuestro grupo ya ha desarrollado un vector eficiente (pBPepGFP2a) basado en el Virus del mosaico de pepino dulce (PepMV; género Potexvirus) para la expresión de proteínas y el silenciamiento génico inducido por virus en Nicotiana benthamiana. El objetivo de este trabajo fue mejorar aún más pBPepGFP2a en términos de idoneidad y estabilidad en infecciones en tomate. Para evitar la recombinación homóloga debido a la duplicación de la secuencia, se diseñaron dos construcciones nuevas con diferente número de mutaciones, pBPepGFPm1 y pBPepGFPm2, utilizando pBPepGFP2a como molde. Los ensayos de estabilidad mostraron que PepGFPm2 era estable en plantas de N. benthamiana durante al menos 3 pases, pero no en plantas de tomate, en las que no se detectó expresión de GFP con ninguno de los vectores. Posteriormente, el gen gfp de pBPepGFPm2 fue reemplazado por los genes DsRed o mCherry, dando lugar a los vectores PepDsRed y PepmCherry. Ambos vectores fueron estables en N. benthamiana y PepDsRed fue también estable en tomate al menos durante dos pases. [ENG] Our group has already developed an efficient Pepino mosaic virus (PepMV; genus Potexvirus)-based vector (pBPepGFP2a) for protein expression and virus induced gene silencing in Nicotiana benthamiana. The aim of this work was to further improve pBPepGFP2a in terms of stability and suitability for reporting infections in tomato. To avoid homologous recombination due to sequence duplication, two new constructs with different number of mutations were designed, pBPepGFPm1 and pBPepGFPm2, using pBPepGFP2a as backbone. Stability assays showed that PepGFPm2 was stable in N. benthamiana plants for at least 3 passages, but not in tomato plants, in which no GFP expression was detected with any of the vectors. Then, the gfp gene from pBPepGFPm2 was replaced by the DsRed or mCherry genes, giving rise to the PepDsRed and PepmCherry vectors. Both vectors were stable in N. benthamiana and PepDsRed was also stable in tomato at least during two passages.Agradezco la financiación de la beca FPI a la Fundación Séneca de la Región de Murcia, y la financiación de los experimentos al proyecto de Plan Estatal AGL2015-65838-R (Ministerio de Economía y Competitividad)

PepGPFm2, PepDsRed and PepBar, a new generation of viral vectors based on pepino mosaic virus

[SPA] Nuestro grupo ya ha desarrollado un vector eficiente (pBPepGFP2a) basado en el virus del mosaico de pepino dulce (PepMV; género Potexvirus) para la expresión de proteínas y el silenciamiento génico inducido por virus en Nicotiana benthamiana. Tras mejorar pJLPepGFP2a en términos de estabilidad e idoneidad para la monitorización de la infección tanto en N. benthamiana como en tomate, los vectores pJLPepGFPm2 y pJLPepDsRed han sido empleados para el estudio de localización subcelular de PepMV. A la vez hemos desarrollado un vector, PepBar, para su empleo en escrutinios masivos de susceptibilidad, en el que el gen gfp de PepGFPm2 fue sustituido por el gen bar, un gen de resistencia a herbicidas. Este vector fue estable durante al menos dos pasajes tanto en N. benthamiana como en plantas de tomate. [ENG] Our group has already developed an efficient Pepino mosaic virus (PepMV; genus Potexvirus)-based vector (pBPepGFP2a) for protein expression and virus-induced gene silencing in Nicotiana benthamiana. We have improved pJLPepGFP2a in terms of stability and suitability for reporting infections in N. benthamiana and tomato, producing pJLPepGFPm2 and pJLPepDsRed. These new vectors have been used for PepMV subcellular localization assays. Moreover, to be used in massive susceptibility screenings, we also developed PepBar, in which the gfp gene from PepGFPm2 was substituted by the bar gene, an herbicide resistance gene. This vector was stable for at less two passages both in N. benthamiana and tomato plants.Agradezco la financiación de la beca FPI a la Fundación Séneca de la Región de Murcia, y la financiación de los experimentos al proyecto de Plan Estatal AGL2015-65838-R (Ministerio de Economía y Competitividad)

e+e- annihilation to (pi0 pi0 gamma) and (pi0 eta gamma) as a source of information on scalar and vector mesons

We present a general framework for the model-independent decomposition of the fully differential cross section of the reactions e+e- -> gamma* -> (pi0 pi0 gamma) and e+e- -> gamma* -> (pi0 eta gamma), which can provide important information on the properties of scalar mesons: f0(600), f0(980) and a0(980). For the model-dependent ingredients in the differential cross section, an approach is developed, which relies on Resonance Chiral Theory with vector and scalar mesons. Numerical results are compared to data. The framework is convenient for development of a Monte Carlo generator and can also be applied to the reaction e+e- -> gamma* -> (pi+ pi- gamma).Comment: 15 pages, 12 Figures, 4 Tables; LaTeX svjour style; update to the version accepted for publication in the European Physical Journal

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results

Observation of a new chi_b state in radiative transitions to Upsilon(1S) and Upsilon(2S) at ATLAS

The chi_b(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider (LHC) at sqrt(s) = 7 TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4 fb^-1, these states are reconstructed through their radiative decays to Upsilon(1S,2S) with Upsilon->mu+mu-. In addition to the mass peaks corresponding to the decay modes chi_b(1P,2P)->Upsilon(1S)gamma, a new structure centered at a mass of 10.530+/-0.005 (stat.)+/-0.009 (syst.) GeV is also observed, in both the Upsilon(1S)gamma and Upsilon(2S)gamma decay modes. This is interpreted as the chi_b(3P) system.Comment: 5 pages plus author list (18 pages total), 2 figures, 1 table, corrected author list, matches final version in Physical Review Letter

Search for displaced vertices arising from decays of new heavy particles in 7 TeV pp collisions at ATLAS

We present the results of a search for new, heavy particles that decay at a significant distance from their production point into a final state containing charged hadrons in association with a high-momentum muon. The search is conducted in a pp-collision data sample with a center-of-mass energy of 7 TeV and an integrated luminosity of 33 pb^-1 collected in 2010 by the ATLAS detector operating at the Large Hadron Collider. Production of such particles is expected in various scenarios of physics beyond the standard model. We observe no signal and place limits on the production cross-section of supersymmetric particles in an R-parity-violating scenario as a function of the neutralino lifetime. Limits are presented for different squark and neutralino masses, enabling extension of the limits to a variety of other models.Comment: 8 pages plus author list (20 pages total), 8 figures, 1 table, final version to appear in Physics Letters

Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC

Measurements of inclusive jet suppression in heavy ion collisions at the LHC provide direct sensitivity to the physics of jet quenching. In a sample of lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the anti-kt algorithm with values for the distance parameter that determines the nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp. Jet production is found to be suppressed by approximately a factor of two in the 10% most central collisions relative to peripheral collisions. Rcp varies smoothly with centrality as characterized by the number of participating nucleons. The observed suppression is only weakly dependent on jet radius and transverse momentum. These results provide the first direct measurement of inclusive jet suppression in heavy ion collisions and complement previous measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables, submitted to Physics Letters B. All figures including auxiliary figures are available at http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02

Reducing heterotic M-theory to five dimensional supergravity on a manifold with boundary

This paper constructs the reduction of heterotic $M$-theory in eleven dimensions to a supergravity model on a manifold with boundary in five dimensions using a Calabi-Yau three-fold. New results are presented for the boundary terms in the action and for the boundary conditions on the bulk fields. Some general features of dualisation on a manifold with boundary are used to explain the origin of some topological terms in the action. The effect of gaugino condensation on the fermion boundary conditions leads to a `twist' in the chirality of the gravitino which can provide an uplifting mechanism in the vacuum energy to cancel the cosmological constant after moduli stabilisation.Comment: 16 pages, RevTe

Measurement of the inclusive isolated prompt photon cross-section in pp collisions at sqrt(s)= 7 TeV using 35 pb-1 of ATLAS data

A measurement of the differential cross-section for the inclusive production of isolated prompt photons in pp collisions at a center-of-mass energy sqrt(s) = 7 TeV is presented. The measurement covers the pseudorapidity ranges |eta|<1.37 and 1.52<=|eta|<2.37 in the transverse energy range 45<=E_T<400GeV. The results are based on an integrated luminosity of 35 pb-1, collected with the ATLAS detector at the LHC. The yields of the signal photons are measured using a data-driven technique, based on the observed distribution of the hadronic energy in a narrow cone around the photon candidate and the photon selection criteria. The results are compared with next-to-leading order perturbative QCD calculations and found to be in good agreement over four orders of magnitude in cross-section.Comment: 7 pages plus author list (18 pages total), 2 figures, 4 tables, final version published in Physics Letters

Measurement of the production cross section of prompt j/psi mesons in association with a W (+/-) boson in pp collisions root s=7 TeV with the ATLAS detector

The process pp → W±J/ψ provides a powerful probe of the production mechanism of charmonium in hadronic collisions, and is also sensitive to multiple parton interactions in the colliding protons. Using the 2011 ATLAS dataset of 4.5 fb-1 of p s = 7TeV pp collisions at the LHC, the first observation is made of the production of W± + prompt J/ events in hadronic collisions, using W± → μ and J/ψ → μ+μ-. A yield of 27.4±7.5 -6.5 W± + prompt J/ψ events is observed, with a statistical significance of 5.1. The production rate as a ratio to the inclusive W± boson production rate is measured, and the double parton scattering contribution to the cross section is estimated. Copyright CERN, for the benefit of the ATLAS Collaboration