The paradox of retained genetic diversity of Hippocampus guttulatus in the face of demographic decline

Genetic diversity is the raw foundation for evolutionary potential. When genetic diversity is significantly reduced, the risk of extinction is heightened considerably. The long-snouted seahorse (Hippocampus guttulatus) is one of two seahorse species occurring in the North-East Atlantic. The population living in the Ria Formosa (South Portugal) declined dramatically between 2001 and 2008, prompting fears of greatly reduced genetic diversity and reduced effective population size, hallmarks of a genetic bottleneck. This study tests these hypotheses using samples from eight microsatellite loci taken from 2001 and 2013, on either side of the 2008 decline. The data suggest that the population has not lost its genetic diversity, and a genetic bottleneck was not detectable. However, overall relatedness increased between 2001 to 2013, leading to questions of future inbreeding. The effective population size has seemingly increased close to the threshold necessary for the population to retain its evolutionary potential, but whether these results have been affected by sample size is not clear. Several explanations are discussed for these unexpected results, such as gene flow, local decline due to dispersal to other areas of the Ria Formosa, and the potential that the duration of the demographic decline too short to record changes in the genetic diversity. Given the results presented here and recent evidence of a second population decline, the precise estimation of both gene flow and effective population size via more extensive genetic screening will be critical to effective population management.16-02-01-FMP-54, MAR-01.04.02-FEAMP-0029, UID/Multi/04326/2020info:eu-repo/semantics/publishedVersio

Renin-Angiotensin System on Reproductive Biology

In the female reproductive system, angiotensin II (ANG II) is a potential signaling molecule involved in ovarian follicle development, which acts through two transmembrane receptors. Within the ovarian follicle, there appear to be species differences in the precise pattern of localization of AGTR2 protein and it has an important role in in vitro maturation of oocytes in mammals. The infusion of ANG II induced ovulation in rabbits and the use of ANG II antagonists inhibited ovulation in rabbits, rats, and cattle. In fetal ovaries, AGTR2 protein was detected in ovigerous cords and preantral follicles throughout porcine and bovine gestation. In the oviduct, ANG II is responsible for the orchestration of the transport of gametes. In the male reproductive system, there is considerable evidence for the local synthesis of components of renin-angiotensin system (RAS) in male reproductive tissues. The roles of RAS in local processes at these sites are still uncertain, although there is evidence for involvement in tubular contractility, spermatogenesis, sperm maturation, capacitation, acrosomal exocytosis, and fertilization

A differential chemical element analysis of the metal poor Globular Cluster NGC 6397

We present chemical abundances in three red giants and two turn-off stars in the metal poor Galactic globular cluster (GC) NGC 6397 based on spectroscopy obtained with the MIKE high resolution spectrograph on the Magellan 6.5-m Clay telescope. Our results are based on a line-by-line differential abundance analysis relative to the well-studied red giant Arcturus and the Galactic halo field star Hip 66815. At a mean of -2.10 +/- 0.02 (stat.) +/- 0.07 (sys.) the differential iron abundance is in good agreement with other studies in the literature based on gf-values. As in previous, differential works we find a distinct departure from ionization equilibrium in that the abundances of Fe I and Fe II differ by ~0.1 dex, with opposite sign for the RGB and TO stars. The alpha-element ratios are enhanced to 0.4 (RGB) and 0.3 dex (TO), respectively, and we also confirm strong variations in the O, Na, and Al/Fe abundance ratios. Accordingly, the light-element abundance patterns in one of the red giants can be attributed to pollution by an early generation of massive SNe II. TO and RGB abundances are not significantly different, with the possible exception of Mg and Ti, which is, however, amplified by the patterns in one TO star, additionally belonging to this early generation of GC stars. We discuss interrelations of these light elements as a function of the GC metallicity.Comment: 12 pages, 7 figures, accepted for publication in the A

The eclipsing millisecond pulsar PSR J1740-5340 and its red straggler companion

We present a high-resolution echelle spectrum taken with the Very Large Telescope and analyse archival Hubble Space Telescope photometry of the recently identified companion of the eclipsing millisecond radio pulsar PSR J1740-5340 in the globular cluster NGC 6397. From the spectrum, we show that the companion is metal poor, as expected for a member of NGC 6397. Using synthetic photometry, and assuming a true distance modulus of 12.13 +/- 0.15 mag and a colour excess of E(B-V) = 0.179, we derive a radius of 1.60 +/- 0.17 solar radii and an effective temperature of 5410 +/- 50 K, implying a luminosity of 2.0 +/- 0.4 solar luminosities (1 sigma errors). These properties make it similar to the so-called `sub-subgiants' in the old open cluster M67 and `red stragglers' in 47 Tuc, which have luminosities comparable to those of turn-off stars, but cooler temperatures and larger radii. The light curve of the companion is well described by ellipsoidal variations, and despite the incomplete (~60%) phase coverage, we are able to derive good constraints on a number of the system parameters. In particular, for the inclination we find a 2 sigma lower limit of i > 48 deg (i > 46 deg at 3 sigma). Assuming a pulsar mass of 1.2 M_MSP < 2.4 solar masses, this implies a companion mass in the range 0.14 < M_comp < 0.38 solar masses. Combined with the photometric constraint, we find a best fit for i ~ 50 deg and M_comp ~ 0.3 solar masses. We infer a Roche lobe filling factor by radius of ~97%. [Abstract shortened]Comment: 12 pages, 6 figures, to appear in A&

Atomic diffusion and mixing in old stars II. Observations of stars in the globular cluster NGC 6397 with VLT/FLAMES-GIRAFFE

Evolutionary trends in the surface abundances of heavier elements have recently been identified in the globular cluster NGC 6397 ([Fe/H]=-2), indicating the operation of atomic diffusion in these stars. Such trends constitute important constraints for the extent to which diffusion modifies the internal structure and surface abundances of solar-type, metal-poor stars. We perform an independent check of the reality and size of abundance variations within this metal-poor globular cluster. Observational data covering a large stellar sample, located between the cluster turn-off point and the base of the red giant branch, are homogeneously analysed. The spectroscopic data were obtained with the medium-high resolution spectrograph FLAMES/GIRAFFE on VLT-UT2. We derive independent effective-temperature scales from profile fitting of Balmer lines and by applying colour-temperature calibrations to Str\"omgren uvby and broad-band BVI photometry. An automated spectral analysis code is used together with a grid of MARCS model atmospheres to derive stellar surface abundances of Mg, Ca, Ti, and Fe. We identify systematically higher iron abundances for more evolved stars. The turn-off point stars are found to have 0.13dex lower surface abundances of iron compared to the coolest, most evolved stars in our sample. There is a strong indication of a similar trend in magnesium, whereas calcium and titanium abundances are more homogeneous. Within reasonable error limits, the obtained abundance trends are in agreement with the predictions of stellar structure models including diffusive processes (sedimentation, levitation), if additional turbulent mixing below the outer convection zone is included.Comment: 11 pages + appendix 6 pages, 9 figures. Accepted for publication in A&

Signatures of intrinsic Li depletion and Li-Na anti-correlation in the metal-poor globular cluster NGC6397

To alleviate the discrepancy between the prediction of the primordial lithium abundance in the universe and the abundances observed in Pop II dwarfs and subgiant stars, it has been suggested that the stars observable today have undergone photospheric depletion of Li. To constrain the nature of such depletion, we conduct a homogeneous analysis of a very large sample of stars in the metal-poor globular cluster NGC6397, covering well all evolutionary phases from below the main sequence turn-off to high up the red giant branch. Non-LTE Li abundances or abundance upper limits are obtained for all stars, and for a size-able sub-set of the targets also Na abundances are obtained. The sodium abundances are used to distinguish stars formed out of pristine material from stars formed out of material affected by pollution from a previous generation of more massive stars. The dwarfs, turn-off, and early subgiant stars form a thin abundance plateau, disrupted in the middle of the subgiant branch by the lithium dilution caused by the first dredge-up. A second steep abundance drop is seen at the red giant branch bump. The turn-off stars are more lithium-poor, by up to 0.1 dex, than subgiants that have not yet undergone dredge-up. In addition, hotter dwarfs are slightly more lithium-poor than cooler dwarfs, which may be a signature of the so-called Li dip in the cluster, commonly seen among PopI stars. A considerably large spread in Na abundance confirms that NGC6397 has suffered from intra-cluster pollution in its infancy and a limited number of Na-enhanced and Li-deficient stars strongly contribute to form a significant anti-correlation between the abundances of Na and Li. The inferred Li abundances are compared to predictions from stellar structure models including atomic diffusion and ad-hoc turbulence below the convection zone.Comment: 13 pages, 11 figures, accepted by A&A, minor language corrections and some references change

The pace of life for forest trees.

Tree growth and longevity trade-offs fundamentally shape the terrestrial carbon balance. Yet, we lack a unified understanding of how such trade-offs vary across the world's forests. By mapping life history traits for a wide range of species across the Americas, we reveal considerable variation in life expectancies from 10 centimeters in diameter (ranging from 1.3 to 3195 years) and show that the pace of life for trees can be accurately classified into four demographic functional types. We found emergent patterns in the strength of trade-offs between growth and longevity across a temperature gradient. Furthermore, we show that the diversity of life history traits varies predictably across forest biomes, giving rise to a positive relationship between trait diversity and productivity. Our pan-latitudinal assessment provides new insights into the demographic mechanisms that govern the carbon turnover rate across forest biomes

Local hydrological conditions influence tree diversity and composition across the Amazon basin

Tree diversity and composition in Amazonia are known to be strongly determined by the water supplied by precipitation. Nevertheless, within the same climatic regime, water availability is modulated by local topography and soil characteristics (hereafter referred to as local hydrological conditions), varying from saturated and poorly drained to well-drained and potentially dry areas. While these conditions may be expected to influence species distribution, the impacts of local hydrological conditions on tree diversity and composition remain poorly understood at the whole Amazon basin scale. Using a dataset of 443 1-ha non-flooded forest plots distributed across the basin, we investigate how local hydrological conditions influence 1) tree alpha diversity, 2) the community-weighted wood density mean (CWM-wd) – a proxy for hydraulic resistance and 3) tree species composition. We find that the effect of local hydrological conditions on tree diversity depends on climate, being more evident in wetter forests, where diversity increases towards locations with well-drained soils. CWM-wd increased towards better drained soils in Southern and Western Amazonia. Tree species composition changed along local soil hydrological gradients in Central-Eastern, Western and Southern Amazonia, and those changes were correlated with changes in the mean wood density of plots. Our results suggest that local hydrological gradients filter species, influencing the diversity and composition of Amazonian forests. Overall, this study shows that the effect of local hydrological conditions is pervasive, extending over wide Amazonian regions, and reinforces the importance of accounting for local topography and hydrology to better understand the likely response and resilience of forests to increased frequency of extreme climate events and rising temperatures

Estimating the global conservation status of more than 15,000 Amazonian tree species

Estimates of extinction risk for Amazonian plant and animal species are rare and not often incorporated into land-use policy and conservation planning. We overlay spatial distribution models with historical and projected deforestation to show that at least 36% and up to 57% of all Amazonian tree species are likely to qualify as globally threatened under International Union for Conservation of Nature (IUCN) Red List criteria. If confirmed, these results would increase the number of threatened plant species on Earth by 22%. We show that the trends observed in Amazonia apply to trees throughout the tropics, and we predict thatmost of the world’s >40,000 tropical tree species now qualify as globally threatened. A gap analysis suggests that existing Amazonian protected areas and indigenous territories will protect viable populations of most threatened species if these areas suffer no further degradation, highlighting the key roles that protected areas, indigenous peoples, and improved governance can play in preventing large-scale extinctions in the tropics in this century