Post-Franco Theatre

In the multiple realms and layers that comprise the contemporary Spanish theatrical landscape, “crisis” would seem to be the word that most often lingers in the air, as though it were a common mantra, ready to roll off the tongue of so many theatre professionals with such enormous ease, and even enthusiasm, that one is prompted to wonder whether it might indeed be a miracle that the contemporary technological revolution – coupled with perpetual quandaries concerning public and private funding for the arts – had not by now brought an end to the evolution of the oldest of live arts, or, at the very least, an end to drama as we know it

Panorama general de la aplicación del derecho a la industria química

El autor resume brevemente algunas de las principales normas que regulan la producción de la industria química en el área jurídica que se ha dado en llamar Derecho Industrial. Es el ámbito legal que abarca la gestión de la seguridad, de la salud y de la protección del medio ambiente en todo lo que la actividad industrial pudiera afectarles: desde el balance medioambiental de la actividad (vertidos, residuos, emisiones, consumos, etc.) hasta el transporte de mercancías peligrosas o la responsabilidad que asume una empresa cuando comercializa un producto clasificado como peligroso. El Derecho tiene mucho que decir en estos ámbitos

Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR

A rapid and easy method was developed in order to amplify long fragments of the genome of potato virus Y (PVY), a virus possesing a 10-kb genomic RNA. The method of immunocapture-RT-PCR was adapted, by using thermostable DNA polymerases with proofreading activity and the proper buffers and cycles, to amplify almost the whole genome of PVY in two fragments (5.6 and 4.3 kb) without purifying virions nor viral RNA. Both fragments were cloned subsequently and their ends sequenced. The method is applicable to the rapid cloning and molecular characterization of the genomes of many other RNA viruses