Use of very short answer questions compared to multiple choice questions in undergraduate medical students: an external validation study

Multiple choice questions (MCQs) offer high reliability and easy machine-marking, but allow for cueing and stimulate recognition-based learning. Very short answer questions (VSAQs), which are open-ended questions requiring a very short answer, may circumvent these limitations. Although VSAQ use in medical assessment increases, almost all research on reliability and validity of VSAQs in medical education has been performed by a single research group with extensive experience in the development of VSAQs. Therefore, we aimed to validate previous findings about VSAQ reliability, discrimination, and acceptability in undergraduate medical students and teachers with limited experience in VSAQs development. To validate the results presented in previous studies, we partially replicated a previous study and extended results on student experiences. Dutch undergraduate medical students (n = 375) were randomized to VSAQs first and MCQs second or vice versa in a formative exam in two courses, to determine reliability, discrimination, and cueing. Acceptability for teachers (i.e., VSAQ review time) was determined in the summative exam. Reliability (Cronbach's & alpha;) was 0.74 for VSAQs and 0.57 for MCQs in one course. In the other course, Cronbach's & alpha; was 0.87 for VSAQs and 0.83 for MCQs. Discrimination (average R-ir) was 0.27 vs. 0.17 and 0.43 vs. 0.39 for VSAQs vs. MCQs, respectively. Reviewing time of one VSAQ for the entire student cohort was & PLUSMN;2 minutes on average. Positive cueing occurred more in MCQs than in VSAQs (20% vs. 4% and 20.8% vs. 8.3% of questions per person in both courses). This study validates the positive results regarding VSAQs reliability, discrimination, and acceptability in undergraduate medical students. Furthermore, we demonstrate that VSAQ use is reliable among teachers with limited experience in writing and marking VSAQs. The short learning curve for teachers, favourable marking time and applicability regardless of the topic suggest that VSAQs might also be valuable beyond medical assessment.Cellular mechanisms in basic and clinical gastroenterology and hepatolog

Progress Report China Potato GAP project; Late blight control, seed quality, storage facilities and sustainability studies in Heilongjiang province and communications

In this report we describe the mid-term results of the R&D program of the PPS Potato GAP China. The aim of the Potato GAP China PPS is to exchange information on GAP in potato production and storage, and to set up experiments and demonstration farms in China with Dutch technology and know-how. This last objective, the setup of Centres of Dutch potato Expertise in China, has not been achieved yet, but still has high priority in 2015. In this report, we describe the results of experiments, investigations and communications within the PPS in 2013 and 2014. The R&D topics are potato late blight disease monitoring and control, potato seed quality evaluation, potato storage investigation and sustainability evaluation of potato production

Meta-analysis of 49 549 individuals imputed with the 1000 Genomes Project reveals an exonic damaging variant in ANGPTL4 determining fasting TG levels

Background So far, more than 170 loci have been associated with circulating lipid levels through genomewide association studies (GWAS). These associations are largely driven by common variants, their function is often not known, and many are likely to be markers for the causal variants. In this study we aimed to identify more new rare and low-frequency functional variants associated with circulating lipid levels. Methods We used the 1000 Genomes Project as a reference panel for the imputations of GWAS data from ~60 000 individuals in the discovery stage and ~90 000 samples in the replication stage. Results Our study resu

Associations of autozygosity with a broad range of human phenotypes

In many species, the offspring of related parents suffer reduced reproductive success, a phenomenon known as inbreeding depression. In humans, the importance of this effect has remained unclear, partly because reproduction between close relatives is both rare and frequently associated with confounding social factors. Here, using genomic inbreeding coefficients (F-ROH) for >1.4 million individuals, we show that F-ROH is significantly associated (p <0.0005) with apparently deleterious changes in 32 out of 100 traits analysed. These changes are associated with runs of homozygosity (ROH), but not with common variant homozygosity, suggesting that genetic variants associated with inbreeding depression are predominantly rare. The effect on fertility is striking: F-ROH equivalent to the offspring of first cousins is associated with a 55% decrease [95% CI 44-66%] in the odds of having children. Finally, the effects of F-ROH are confirmed within full-sibling pairs, where the variation in F-ROH is independent of all environmental confounding.Peer reviewe

The Influence of Age and Sex on Genetic Associations with Adult Body Size and Shape : A Large-Scale Genome-Wide Interaction Study

Genome-wide association studies (GWAS) have identified more than 100 genetic variants contributing to BMI, a measure of body size, or waist-to-hip ratio (adjusted for BMI, WHRadjBMI), a measure of body shape. Body size and shape change as people grow older and these changes differ substantially between men and women. To systematically screen for age-and/or sex-specific effects of genetic variants on BMI and WHRadjBMI, we performed meta-analyses of 114 studies (up to 320,485 individuals of European descent) with genome-wide chip and/or Metabochip data by the Genetic Investigation of Anthropometric Traits (GIANT) Consortium. Each study tested the association of up to similar to 2.8M SNPs with BMI and WHRadjBMI in four strata (men 50y, women 50y) and summary statistics were combined in stratum-specific meta-analyses. We then screened for variants that showed age-specific effects (G x AGE), sex-specific effects (G x SEX) or age-specific effects that differed between men and women (G x AGE x SEX). For BMI, we identified 15 loci (11 previously established for main effects, four novel) that showed significant (FDR= 50y). No sex-dependent effects were identified for BMI. For WHRadjBMI, we identified 44 loci (27 previously established for main effects, 17 novel) with sex-specific effects, of which 28 showed larger effects in women than in men, five showed larger effects in men than in women, and 11 showed opposite effects between sexes. No age-dependent effects were identified for WHRadjBMI. This is the first genome-wide interaction meta-analysis to report convincing evidence of age-dependent genetic effects on BMI. In addition, we confirm the sex-specificity of genetic effects on WHRadjBMI. These results may providefurther insights into the biology that underlies weight change with age or the sexually dimorphism of body shape.Peer reviewe

CHANGE IN N-TERMINAL PRO B-TYPE NATRIURETIC PEPTIDE LEVELS IN OLD AGE: THE LEIDEN 85-PLUS STUDY

Pathophysiology, epidemiology and therapy of agein

A crop production ecology (CPE) approach to sustainable production of biomass for food, feed and fuel

With the rapid increase in demand for agricultural products for food, feed and fuel, concerns are growing about sustainability issues. Can agricultural production meet the needs of increasing numbers of people consuming more animal products and using a larger share of crops as fuel for transport, electricity and heat, while still sustaining the natural resource base? In addition to economic models and learning from statistics and trends, there is a perceived need for decision support tools at global, field and plant levels and for the certification of best practices based on crop production ecology (CPE). This paper illustrates the need for and availability of a generic approach to sustainability principles, criteria, indicators and norms to ensure maximum efficiency in the use of resources such as land, water, chemicals and energy in crop biomass production at various levels of scale. The authors propose a method based on a transportable CPE approach, covering ranges of commodities and environments, to address choices in agricultural production: which crop to promote where, how it should be grown to optimize the efficient use of resources, how to certify the best practices and which crop properties need genetic improvement to make the best use of scarce resources in adverse conditions

From food safety guidelines to quantified sustainability indicators: A transition to good practice schemes in food production in Europe

Over the last decade and a half various aspects of the sustainability of food production have been evaluated by different groups of stakeholders. National and federal legislators have looked at the types of chemicals allowed and the acceptable maximum residue levels permitted for particular crops and conditions. Commercial firms (in both the processing and retail sectors) have developed private company labels that distinguish them from competitors. Their suppliers have to comply under a 'licence to deliver'. Some organizations that are concerned with environmental issues have developed guidelines. Finally, producer organizations have developed their own labels. The assumption is that farmers who comply have products with added value, which give rise to better opportunities to sell. Some processors source only from growers complying with such schemes. This paper highlights past endeavours, rules and tools to assure food safety, and present initiatives to bring sustainability into the pre-competitive domain. It also identifies future requirements for sustainability indicators and baseline studies, and demonstrates the need to move from indicator values to target values. This approach allows for the quantitative monitoring of environmental safety that will be required for the food industry of the future, just as food safety is now and was in the past