European union : labyrinth or reality? : European union and integration, 1969-1986

European Union is not a new or easily defined concept. Implicit in the Treaty of Rome - the founding Treaty of the European Community - is the intention of a federal form of political union. This union is to be achieved by a gradualist strategy of building solidarity among European people, beginning in the economic sphere. Using this historical perspective, this thesis examines European Union proposals from 1969 to 1986 in order to facilitate an understanding of them in terms of integration. The European Union proposals are found to contain little more than a reconfirmation or extension of the guidelines envisaged in the Treaty of Rome. Common themes are identified as the foci for areas where European Union can legitimately emerge or be expanded. These themes are related to integration approaches applicable to European Community experience. While no one integration approach can account for European Community reality, each approach highlights different unifying conditions and strategies which promote or hinder progress towards European Union. A case study of the European Parliament's draft European Union Treaty will relate the European Union proposals to the integration approaches in order to establish the current status of the European Community, as well as to suggest possible paths for the development of European Union

Abundances of Extremely Metal-Poor Star Candidates

We present chemical abundances for 110 stars identified in objective-prism surveys as candidates to be very metal-poor. The abundances are derived from high S/N, intermediate-resolution spectra obtained with the Keck Observatory Echelle Spectrometer and Imager. An additional 25 stars with well-determined abundances ranging from [Fe/H]$=-1.5$ and -3.2 were observed and the results used to help calibrate our analysis and determine the accuracy of our abundance determinations. Abundances for the program stars were measured for Fe, Mg, Ca, Ti, Cr and Ba with an accuracy of approximately 0.3 dex. 53 of the stars in our sample have [Fe/H]<= -2, 22 have [Fe/H]<= -2.5 and 13 stars have [Fe/H]<= -2.9. Surprisingly, approximately one third of the sample is relatively metal rich with [Fe/H]>-1.5. In addition to identifying a number of extremely metal-poor stars, this study also shows that moderate-resolution spectra obtained with the Keck Echelle Spectrometer and Imager yield relatively accurate abundances for stars as faint as V=14 in modest exposure time (~20 minutes). This capability will prove useful if the so-far elusive stars at [Fe/H]<-4 turn out to be mostly fainter than V=15.Comment: 60 pages, 10 figures, Accepted for publication in the A

Stellar Archaeology: a Keck Pilot Program on Extremely Metal- Poor Stars From the Hamburg/ESO Survey. III. The Lead (Pb) Star HE 0024-2523

We present a detailed abundance analysis, including spectral syntheses, of a very metal-poor ([Fe/H]= -2.7), peculiar main sequence star, HE0024-2523 detected during the course of the Keck Pilot Program. Radial velocities of this star were obtained during four different observing runs over a time span of 1.1 years, and demonstrate that it is clearly a short period spectroscopic binary. An orbital solution was obtained, and orbital parameters were determined with high precision. The rotational velocity was also measured (vsin i=9.7$\pm$1.5 kms); rotation appears likely to be synchronous with the orbit. The abundance analysis and spectral syntheses indicate that the object is a CH star characterized by extreme s-process enrichment, likely due to mass accretion from an evolved companion which has now probably become a white dwarf. The lead (Pb) abundance of HE0024-2523 is very high, the same as that of the recently discovered lead-rich metal-poor star CS 29526-110, [Pb/Fe]=+3.3. The abundance ratio of the heavy-s to light-s elements, as characterized by Pb and Ba, [Pb/Ba]=+1.9, is the highest yet found for any metal-poor star, and is about 0.7 dex higher than that of CS29526-110. On the basis of the measured isotopic ratio of carbon (12C/13C about 6) we argue that the mass donor must have had an original mass of at least 3 Msun. The unusually short period of this CH star suggests that it underwent a past common-envelope phase with its evolved companion. Our results are compared to the latest available models for AGB yields and s-process nucleosynthesis. We also discuss the possible connection between HE0024-2523 the lithium depletion of halo stars, and halo blue straggler formation.Comment: 55 pages, 17 color figures included; Accepted for publication in the February 2003 issue of the Astronomical Journa

Building for the future: essential infrastructure for rodent ageing studies

When planning ageing research using rodent models, the logistics of supply, long term housing and infrastructure provision are important factors to take into consideration. These issues need to be prioritised to ensure they meet the requirements of experiments which potentially will not be completed for several years. Although these issues are not unique to this discipline, the longevity of experiments and indeed the animals, requires a high level of consistency and sustainability to be maintained throughout lengthy periods of time. Moreover, the need to access aged stock or material for more immediate experiments poses many issues for the completion of pilot studies and/or short term intervention studies on older models. In this article, we highlight the increasing demand for ageing research, the resources and infrastructure involved, and the need for large-scale collaborative programmes to advance studies in both a timely and a cost-effective way

Genetic risk and a primary role for cell-mediated immune mechanisms in multiple sclerosis.

Multiple sclerosis is a common disease of the central nervous system in which the interplay between inflammatory and neurodegenerative processes typically results in intermittent neurological disturbance followed by progressive accumulation of disability. Epidemiological studies have shown that genetic factors are primarily responsible for the substantially increased frequency of the disease seen in the relatives of affected individuals, and systematic attempts to identify linkage in multiplex families have confirmed that variation within the major histocompatibility complex (MHC) exerts the greatest individual effect on risk. Modestly powered genome-wide association studies (GWAS) have enabled more than 20 additional risk loci to be identified and have shown that multiple variants exerting modest individual effects have a key role in disease susceptibility. Most of the genetic architecture underlying susceptibility to the disease remains to be defined and is anticipated to require the analysis of sample sizes that are beyond the numbers currently available to individual research groups. In a collaborative GWAS involving 9,772 cases of European descent collected by 23 research groups working in 15 different countries, we have replicated almost all of the previously suggested associations and identified at least a further 29 novel susceptibility loci. Within the MHC we have refined the identity of the HLA-DRB1 risk alleles and confirmed that variation in the HLA-A gene underlies the independent protective effect attributable to the class I region. Immunologically relevant genes are significantly overrepresented among those mapping close to the identified loci and particularly implicate T-helper-cell differentiation in the pathogenesis of multiple sclerosis

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

A nontoxic polypeptide oligomer with a fungicide potency under agricultural conditions which is equal or greater than that of their chemical counterparts

Research ArticleThere are literally hundreds of polypeptides described in the literature which exhibit fungicide activity. Tens of them have had attempted protection by patent applications but none, as far as we are aware, have found application under real agricultural conditions. The reasons behind may be multiple where the sensitivity to the Sun UV radiation can come in first place. Here we describe a multifunctional glyco-oligomer with 210 kDa which is mainly composed by a 20 kDa polypeptide termed Blad that has been previously shown to be a stable intermediary product of β-conglutin catabolism. This oligomer accumulates exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination. Blad-oligomer reveals a plethora of biochemical properties, like lectin and catalytic activities, which are not unusual per si, but are remarkable when found to coexist in the same protein molecule. With this vast range of chemical characteristics, antifungal activity arises almost as a natural consequence. The biological significance and potential technological applications of Blad-oligomer as a plant fungicide to agriculture, its uniqueness stems from being of polypeptidic in nature, and with efficacies which are either equal or greater than the top fungicides currently in the market are addressedinfo:eu-repo/semantics/publishedVersio

Biochar successfully replaces activated charcoal for in vitro culture of two white poplar clones reducing ethylene concentration

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The impact of COVID-19 school disruptions on children’s learning

IntroductionNational health policies to stop the spread of the COVID-19 virus in the US resulted in widespread school closures and disrupted learning in Spring 2020.MethodsThis study draws on unique individual-level data from n = 282 5–12 year olds enrolled in the NIH Environmental influences on Child Health Outcomes (ECHO) Research Program to investigate associations between caregiver-reported duration of Spring 2020 learning disruptions and academic achievement.ResultsLinear regression analyses estimated that children who experienced more than 4 weeks of instruction disruptions in Spring 2020 scored 4.5 points [95% CI: −8.77, −0.22] lower on age-normed math assessments compared to peers who had four or fewer weeks of disruption, adjusting for sociodemographic variables, pre-pandemic vocabulary, and COVID-19 family hardships and stress. No differences were found for reading. Children whose caregivers had higher levels of pandemic-related traumatic stress and lower educational attainment also had lower math scores, adjusting for all other covariates.DiscussionResults suggest educators and schools focus additional attention on supporting math instruction for children who experienced extended learning disruptions

Pharmacogenetic meta-analysis of genome-wide association studies of LDL cholesterol response to statins

Statins effectively lower LDL cholesterol levels in large studies and the observed interindividual response variability may be partially explained by genetic variation. Here we perform a pharmacogenetic meta-analysis of genome-wide association studies (GWAS) in studies addressing the LDL cholesterol response to statins, including up to 18,596 statin-treated subjects. We validate the most promising signals in a further 22,318 statin recipients and identify two loci, SORT1/CELSR2/PSRC1 and SLCO1B1, not previously identified in GWAS. Moreover, we confirm the previously described associations with APOE and LPA. Our findings advance the understanding of the pharmacogenetic architecture of statin response