Impact of Embalica Officinalis: A miracle multipurpose plant

Plants are a major source of nutrition and health care. Embalicalofficinalis is one of the most widely used in traditional Indian medicine in different form and believed to alleviate against several disease. This article summarizes multipurpose and medicinal value of Embalicaofficinalis (EO). I reviewed the application of EO in antioxidant, antidiabetic, anticancer, antiulcer, cardioprotective activity, cytoprotective, antitussive, immunomodualation, chelating agent, and respiratory problems

Pathways to child and adolescent psychiatric care in the division of child and adolescent psychiatry at Red Cross Children's Hospital

Objective: To document the routes taken by patients to reach the Division of Child and Adolescent Psychiatry (DCAP) at Red Cross Children’s Hospital and to identify the factors that are associated with delay in gaining access to care

Comparative Study of Underground Pit Grain Storage System through Use of Different Lining Materials

Sorghum occupies highest area of cultivation in East and West Hararghe of Ethiopia. Farmers in the area are poor and do not afford an advanced storage systems. Hence, storage is done mainly in underground pit storage with different lining materials. This study was therefore aimed at evaluating those different lining materials in maintaining the sorghum quality. Four different lining materials which are commonly used by the farmers (mud/straw/cow dung mixture (3:3:1 ratio), plastic (thick rubberized canvas), chaff and cement) were used. Ten pits (duplicated), flask shaped with 1 m depth and 1 m diameter with a capacity of about two quintals (200 kg) were constructed. The finding in this comparative study is that, among all underlining materials, underground pit with plastic lining material was good barrier compared to all linings. Moisture migration to the grain increased in each month in all lining materials and the least was recorded in plastic lining. Germination capacity and thousand grain weight were reduced significantly in each material except plastic lining which showed the least change compared to the others. Fungi infestation was also increased in all underground pits with different lining materials. However, lower infestation was found in plastic and cement linings. Based on the quality measurements, plastic lining showed the least change in grain quality compared to the others. It can be concluded that, since the farmers can’t afford advanced storage system, it can be recommended to use plastic lining material among the existing lining materials

Attitude and its associated factors among adults towards traditional eye medicine in Gondar City, Northwest Ethiopia : a cross-sectional study

This study is to assess attitude and its associated factors among adults towards traditional eye medicine in Gondar city, North West Ethiopia. Traditional medicines are commonly practiced in Africa. It is believed to be alternative health care used in the developing world. It is a more widely available and affordable alternative to pharmaceutical drugs. In Africa, about 13.2- 82.3% of the population utilizes traditional eye medicine. A community-based cross-sectional study was conducted on 600 study participants using a pre-tested structured questionnaire. Data analysis was performed using Statistical Package for Social Sciences (SPSS) version 20 computer software. Association and strength between the outcome variable and independent variables were determined using the odds ratio with a 95 % confidence interval. A total of 633 subjects were included in the study with a 95 % response rate. Of the total study subjects, 292 (48.7 %) (95% CI: 44.7-52.7%)) had a good attitude towards traditional eye medicine use. Variables like being male (AOR=2.00(95% CI: 1.23-2.68)), family history of traditional eye medicine use (AOR=3.31(95% CI: 2.01-5.47)), availability of traditional healer (AOR=1.81(95% CI: 1.12-2.95)) and absence of health insurance (AOR=1.63(95% CI: 1.14-2.55)) were variables significantly associated with good attitude towards traditional eye medicine use. The result of this study shows that attitude towards traditional eye medicine use for the treatment of eye disease was fairly good. It indicates that almost half of the study participants had a good attitude towards traditional eye medicine use. Community awareness programme through collaboration of the health ministry and traditional healers on traditional eye medicine use can help to promote the right perception and reduce the risk of complications

Improving the annotation of the Heterorhabditis bacteriophora genome

Background: Genome assembly and annotation remain exacting tasks. As the tools available for these tasks improve, it is useful to return to data produced with earlier techniques to assess their credibility and correctness. The entomopathogenic nematode Heterorhabditis bacteriophora is widely used to control insect pests in horticulture. The genome sequence for this species was reported to encode an unusually high proportion of unique proteins and a paucity of secreted proteins compared to other related nematodes. Findings: We revisited the H. bacteriophora genome assembly and gene predictions to determine whether these unusual characteristics were biological or methodological in origin. We mapped an independent resequencing dataset to the genome and used the blobtools pipeline to identify potential contaminants. While present (0.2% of the genome span, 0.4% of predicted proteins), assembly contamination was not significant. Conclusions: Re-prediction of the gene set using BRAKER1 and published transcriptome data generated a predicted proteome that was very different from the published one. The new gene set had a much reduced complement of unique proteins, better completeness values that were in line with other related species’ genomes, and an increased number of proteins predicted to be secreted. It is thus likely that methodological issues drove the apparent uniqueness of the initial H. bacteriophora genome annotation and that similar contamination and misannotation issues affect other published genome assemblies

Insect pathogens as biological control agents: back to the future

The development and use of entomopathogens as classical, conservation and augmentative biological control agents have included a number of successes and some setbacks in the past 15 years. In this forum paper we present current information on development, use and future directions of insect-specific viruses, bacteria, fungi and nematodes as components of integrated pest management strategies for control of arthropod pests of crops, forests, urban habitats, and insects of medical and veterinary importance. Insect pathogenic viruses are a fruitful source of MCAs, particularly for the control of lepidopteran pests. Most research is focused on the baculoviruses, important pathogens of some globally important pests for which control has become difficult due to either pesticide resistance or pressure to reduce pesticide residues. Baculoviruses are accepted as safe, readily mass produced, highly pathogenic and easily formulated and applied control agents. New baculovirus products are appearing in many countries and gaining an increased market share. However, the absence of a practical in vitro mass production system, generally higher production costs, limited post application persistence, slow rate of kill and high host specificity currently contribute to restricted use in pest control. Overcoming these limitations are key research areas for which progress could open up use of insect viruses to much larger markets. A small number of entomopathogenic bacteria have been commercially developed for control of insect pests. These include several Bacillus thuringiensis sub-species, Lysinibacillus (Bacillus) sphaericus, Paenibacillus spp. and Serratia entomophila. B. thuringiensis sub-species kurstaki is the most widely used for control of pest insects of crops and forests, and B. thuringiensis sub-species israelensis and L. sphaericus are the primary pathogens used for medically important pests including dipteran vectors,. These pathogens combine the advantages of chemical pesticides and microbial control agents (MCAs): they are fast acting, easy to produce at a relatively low cost, easy to formulate, have a long shelf life and allow delivery using conventional application equipment and systemics (i.e. in transgenic plants). Unlike broad spectrum chemical pesticides, B. thuringiensis toxins are selective and negative environmental impact is very limited. Of the several commercially produced MCAs, B. thuringiensis (Bt) has more than 50% of market share. Extensive research, particularly on the molecular mode of action of Bt toxins, has been conducted over the past two decades. The Bt genes used in insect-resistant transgenic crops belong to the Cry and vegetative insecticidal protein families of toxins. Bt has been highly efficacious in pest management of corn and cotton, drastically reducing the amount of broad spectrum chemical insecticides used while being safe for consumers and non-target organisms. Despite successes, the adoption of Bt crops has not been without controversy. Although there is a lack of scientific evidence regarding their detrimental effects, this controversy has created the widespread perception in some quarters that Bt crops are dangerous for the environment. In addition to discovery of more efficacious isolates and toxins, an increase in the use of Bt products and transgenes will rely on innovations in formulation, better delivery systems and ultimately, wider public acceptance of transgenic plants expressing insect-specific Bt toxins. Fungi are ubiquitous natural entomopathogens that often cause epizootics in host insects and possess many desirable traits that favor their development as MCAs. Presently, commercialized microbial pesticides based on entomopathogenic fungi largely occupy niche markets. A variety of molecular tools and technologies have recently allowed reclassification of numerous species based on phylogeny, as well as matching anamorphs (asexual forms) and teleomorphs (sexual forms) of several entomopathogenic taxa in the Phylum Ascomycota. Although these fungi have been traditionally regarded exclusively as pathogens of arthropods, recent studies have demonstrated that they occupy a great diversity of ecological niches. Entomopathogenic fungi are now known to be plant endophytes, plant disease antagonists, rhizosphere colonizers, and plant growth promoters. These newly understood attributes provide possibilities to use fungi in multiple roles. In addition to arthropod pest control, some fungal species could simultaneously suppress plant pathogens and plant parasitic nematodes as well as promote plant growth. A greater understanding of fungal ecology is needed to define their roles in nature and evaluate their limitations in biological control. More efficient mass production, formulation and delivery systems must be devised to supply an ever increasing market. More testing under field conditions is required to identify effects of biotic and abiotic factors on efficacy and persistence. Lastly, greater attention must be paid to their use within integrated pest management programs; in particular, strategies that incorporate fungi in combination with arthropod predators and parasitoids need to be defined to ensure compatibility and maximize efficacy. Entomopathogenic nematodes (EPNs) in the genera Steinernema and Heterorhabditis are potent MCAs. Substantial progress in research and application of EPNs has been made in the past decade. The number of target pests shown to be susceptible to EPNs has continued to increase. Advancements in this regard primarily have been made in soil habitats where EPNs are shielded from environmental extremes, but progress has also been made in use of nematodes in above-ground habitats owing to the development of improved protective formulations. Progress has also resulted from advancements in nematode production technology using both in vivo and in vitro systems; novel application methods such as distribution of infected host cadavers; and nematode strain improvement via enhancement and stabilization of beneficial traits. Innovative research has also yielded insights into the fundamentals of EPN biology including major advances in genomics, nematode-bacterial symbiont interactions, ecological relationships, and foraging behavior. Additional research is needed to leverage these basic findings toward direct improvements in microbial control

Prevalence and associated factors of myopia among high school students in Gondar town, northwest Ethiopia, 2016

Gizachew Tilahun Belete,1 Dereje Hayilu Anbesse,1 Adino Tesfahun Tsegaye,2 Mohammed Seid Hussen1 1Department of Optometry, 2Department of Biostatistics and Epidemiology, Institute of Public Health, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia Introduction: Myopia is an important cause of correctable visual impairment and preventable blindness worldwide. Prevalence rates are approximately 20%–35% among the older teenage population globally. It has a medical burden of pathologic complications such as maculopathy and glaucomatous optic neuropathy. High school students experience high-performance and study pressures in the preparation for the final national examination. As a result they are exposed to excessive near work and indoor activities. They are also ignored for regular screening. Objective: To determine the prevalence and associated factors of myopia among high school students in Gondar town, Ethiopia. Methods: An institution-based cross-sectional study was conducted on 498 high school students with systematic random sampling method from April 18 to April 29, 2016, in three full-cycle high schools (9th–12th grades). A standardized structured questionnaire, Snellen acuity chart, pinhole, retinoscope, trial case lenses, pen torch, and direct ophthalmoscope were used to collect data. Results: A total of 495 study participants were included, and they had a mean age of 17.48±1.59 years. The prevalence of myopia was 11.9% (95% confidence interval [CI]: 10.2, 17.9). Family history of myopia (adjusted odds ratio [AOR]=8.08 [95% CI: 4.30, 15.16]), school being private (AOR=2.88 [95% CI: 1.02, 8.11]), longer time spent for near work (AOR=2.89 [95% CI: 1.12, 7.43]), longer time spent partaking in indoor activities (AOR=4.32 [95% CI: 1.69, 10.99]), shorter near working distance (AOR=3.06 [95% CI: 1.33, 7.06]), lack of outdoor sport activities (AOR=2.27 [95% CI: 1.05, 4.90]), use of visual display units (AOR=2.81 [95% CI: 1.30, 6.10]), and abnormal ocular findings (AOR=6.69 [CI: 3.43, 13.03]) were found to be independently associated with myopia. Conclusion: The prevalence of myopia was 11.9%. Family history for myopia, school being private, longer time spent partaking in indoor activities, shorter working distance, lack of outdoor sport activities, use of visual display units, and presence of abnormal ocular findings were positively associated with myopia. Keywords: myopia, high school students, Gondar town, Ethiopi