Influencia de las buenas prácticas éticas en los procedimientos de bioseguridad en los laboratorios clínicos del cantón la Concordia de la provincia de Danto Domingo de los Tsáchilas - Ecuador en el periodo 2023

The article addresses the relevance of good ethical practices in clinical laboratories to promote biosafety in the year 2023; especially as it relates to the promotion of biosafety. It indicates the relevance of ethics in the effective execution of biosafety procedures, ensuring the protection of both personnel and patients. It also analyzes professional conduct, which focuses on critical aspects such as patient confidentiality and informed consent as fundamental pillars of ethical practice. It also recognizes that the handling of hazardous samples in these laboratory environments and emphasizes the imperative need to implement preventive biosafety measures. It highlights the direct influence that ethics has on decision making and the resolution of work dilemmas, all based on bioethical principles. This direct connection between ethics and biosafety aims to maximize the benefits and minimize the risks or harms involved. This article draws on a literature review to provide valuable insights into how good ethical practices have a significant impact on biosafety procedures in clinical laboratories. Finally, it poses a key question: What is the influence of good ethical practices on biosafety procedures performed in the clinical laboratory? This question seeks to guide the understanding of how ethical practices not only improve safety, but also quality in these settings, providing a comprehensive approach to medical care and protection of personnel involved in such laboratories.El artículo aborda la relevancia de las buenas prácticas éticas en laboratorios clínicos para promover la bioseguridad en el año 2023; especialmente en lo relacionado con la promoción de la bioseguridad. Indica su relevancia de la ética en la ejecución efectiva de los procedimientos de bioseguridad, asegurando la protección tanto del personal como de los pacientes. Además, analizar la conducta profesional, la cual enfoca aspectos críticos como la confidencialidad del paciente y el consentimiento informado como pilares fundamentales en la práctica ética. Además, reconoce que la manipulación de muestras peligrosas en estos entornos de laboratorio y enfatiza la necesidad imperativa de implementar medidas preventivas de bioseguridad. Se resalta la influencia directa que tiene la ética en la toma de decisiones y en la resolución de dilemas laborales, todo basado en principios bioéticos. Esta conexión directa entre ética y bioseguridad apunta a maximizar los beneficios y minimizar los riesgos o daños involucrados. El presente artículo se apoya en una revisión bibliográfica para aportar perspectivas valiosas que evidencian cómo las buenas prácticas éticas impactan de manera significativa en los procedimientos de bioseguridad en laboratorios clínicos. Por último, plantea una pregunta clave: ¿Cuál es la influencia de las buenas prácticas éticas en los procedimientos de bioseguridad que se realizan en el laboratorio clínico? Esta interrogante busca guiar la comprensión sobre cómo las prácticas éticas no solo mejoran la seguridad, sino también la calidad en estos entornos, proporcionando un enfoque integral para la atención médica y la protección del personal involucrado en dichos laboratorios. &nbsp

APLICACIÓN MÓVIL PARA LA ADQUISICIÓN DE LENGUAJE EN NIÑOS CON TRASTORNO DE HABLA

ResumenEn el estado de Chiapas, México el 9.4% de la población con discapacidad presenta limitaciones en el habla. Esta limitación ocupa el cuarto lugar de discapacidad. Uno de los servicios ofrecidos por el gobierno para este grupo son las sesiones de terapia de lenguaje orientada a niños con discapacidad del habla y aprendizaje del lenguaje. Esto mediante un tratamiento de rehabilitación a base de ejercicios fono-articuladores. Cada niño necesita de ejercicios diferentes para el desarrollo de su lenguaje oral, con el que podrá expresar sus necesidades, pensamientos, sentimientos y emociones. El presente trabajo apoya al proceso de adquisición del habla por medio de un software especializado para dispositivos móviles con sistema operativo Android, que integra actividades de terapia para el desarrollo de lenguaje en niños de nivel preescolar con trastornos de habla. Las actividades son presentadas usando el lenguaje coloquial propio de la región. Esto permite al niño adquirir el habla de una forma agradable en un entorno familiar. Esta aplicación ofrece sesiones especializadas que incluyen actividades usando el lenguaje y los juegos propios de la región que promueven la adquisición del habla en niños de 3 a 6 años de edad con trastorno de habla en el estado de Chiapas, México.Palabras Clave: dispositivos móviles, software educativo, trastorno de habla. AbstractIn Mexico in the state of Chiapas 9.4% of the disabled population has limitation in speech. This limitation is the fourth of disability presented in this state. One of the services offered by the government for this group are oriented therapy sessions for children with speech and language learning disabilities. This by a rehabilitative treatment based on speech articulation. Every child needs different exercises for the development of oral language, with which you can express their needs, thoughts, feelings and emotions. This work supports the process of acquiring speech by specialized software. This is designed for mobile devices with Android operating system and integrates therapy activities for the acquisition of language in preschool children with speech disorders. The activities are presented using the own colloquial language of the state of Chiapas. This allows the child to acquire speech in a pleasant way in a familiar environment. This application provides specialized sessions including activities using language and own game in the region to promote the acquisition of speech in children 3 to 6 years old with speech disorder in the state of Chiapas, Mexico.Keywords: mobile devices, educational software, speech disorder

Water absorption and degradation characteristics of chitosan-based polyesters and hydroxyapatite composites

Blends of chitosan and biodegradable synthetic aliphatic polyesters (polycaprolactone, poly-(butylene succinate), poly[(butylene succinate)-co-adipate], poly[(butylene terephthalate)-co-adipate], and poly(lactic acid)) were injection-molded. These samples were immersed in isotonic solution at 37ºC for a period of 60 d. The water uptake and the degradation properties, as measured by the loss in tensile strength, were evaluated as a function of time. In this study, the rate and the equilibriumwater uptake were proportional to the amount of chitosan in the blend. The addition of HA to chitosan and polyester significantly reduced the equilibrium water uptake. The water uptake did not follow the classical Fickian phenomena and could be expressed by a two-stage sorption non-Fickian diffusion model. Contact angle measurement was used to quantify the changes in surface hydrophilicity as a function of chitosan and polyester composition. The glycerol contact angle decreased with increasing synthetic components in the blend. The blends and composites also showed increased degradation, as quantified by a loss in their mechanical properties, with increase in natural content. The degradation of properties was directly related to the water uptake of the blends; the higher the water uptake, the higher the degradation. Pure polyesters, while having low water uptake, nevertheless showed significant degradation by a precipitous drop in the strain at break. Among the polyesters, poly(lactic acid) displayed maximum degradation, while polycaprolactone displayed the least.Blends of chitosan and biodegradable synthetic aliphatic polyesters (polycaprolactone, poly(butylene succinate), poly[(butylene succinate)-co-adipate], poly[(butylene terephthalate)co-adipate], and poly(lactic acid)) were injection-molded. These samples were immersed in isotonic solution at 37 degrees C for a period of 60 d. The water uptake and the degradation properties, as measured by the loss in tensile strength, were evaluated as a function of time. In this study, the rate and the equilibrium water uptake were proportional to the amount of chitosan in the blend. The addition of HA to chitosan and polyester significantly reduced the equilibrium water uptake. The water uptake did not follow the classical Fickian phenomena and could be expressed by a two-stage sorption non-Fickian diffusion model. Contact angle measurement was used to quantify the changes in surface hydrophilicity as a function of chitosan and polyester composition. The glycerol contact angle decreased with increasing synthetic components in the blend. The blends and composites also showed increased degradation, as quantified by a loss in their mechanical properties, with increase in natural content. The degradation of properties was directly related to the water uptake of the blends; the higher the water uptake, the higher the degradation. Pure polyesters, while having low water uptake, nevertheless showed significant degradation by a precipitous drop in the strain at break. Among the polyesters, poly(lactic acid) displayed maximum degradation, while polycaprolactone displayed the least

The GRAVITY Coud\'e Infrared Adaptive Optics (CIAO) system for the VLT Interferometer

GRAVITY is a second generation instrument for the VLT Interferometer, designed to enhance the near-infrared astrometric and spectro-imaging capabilities of VLTI. Combining beams from four telescopes, GRAVITY will provide an astrometric precision of order 10 micro-arcseconds, imaging resolution of 4 milli-arcseconds, and low and medium resolution spectro-interferometry, pushing its performance far beyond current infrared interfero- metric capabilities. To maximise the performance of GRAVITY, adaptive optics correction will be implemented at each of the VLT Unit Telescopes to correct for the effects of atmospheric turbulence. To achieve this, the GRAVITY project includes a development programme for four new wavefront sensors (WFS) and NIR-optimized real time control system. These devices will enable closed-loop adaptive correction at the four Unit Telescopes in the range 1.4-2.4 {\mu}m. This is crucially important for an efficient adaptive optics implementation in regions where optically bright references sources are scarce, such as the Galactic Centre. We present here the design of the GRAVITY wavefront sensors and give an overview of the expected adaptive optics performance under typical observing conditions. Benefiting from newly developed SELEX/ESO SAPHIRA electron avalanche photodiode (eAPD) detectors providing fast readout with low noise in the near-infrared, the AO systems are expected to achieve residual wavefront errors of \leq400 nm at an operating frequency of 500 Hz.Comment: to be published in Proc. SPIE vol. 8446 (2012

The genome sequencing of an albino Western lowland gorilla reveals inbreeding in the wild

Background The only known albino gorilla, named Snowflake, was a male wild born individual from Equatorial Guinea who lived at the Barcelona Zoo for almost 40 years. He was diagnosed with non-syndromic oculocutaneous albinism, i.e. white hair, light eyes, pink skin, photophobia and reduced visual acuity. Despite previous efforts to explain the genetic cause, this is still unknown. Here, we study the genetic cause of his albinism and making use of whole genome sequencing data we find a higher inbreeding coefficient compared to other gorillas. Results We successfully identified the causal genetic variant for Snowflake¿s albinism, a non-synonymous single nucleotide variant located in a transmembrane region of SLC45A2. This transporter is known to be involved in oculocutaneous albinism type 4 (OCA4) in humans. We provide experimental evidence that shows that this amino acid replacement alters the membrane spanning capability of this transmembrane region. Finally, we provide a comprehensive study of genome-wide patterns of autozygogosity revealing that Snowflake¿s parents were related, being this the first report of inbreeding in a wild born Western lowland gorilla. Conclusions In this study we demonstrate how the use of whole genome sequencing can be extended to link genotype and phenotype in non-model organisms and it can be a powerful tool in conservation genetics (e.g., inbreeding and genetic diversity) with the expected decrease in sequencing cost. Keywords: Gorilla; Albinism; Inbreeding; Genome; Conservatio

Tele-entomology and tele-parasitology: A citizen science-based approach for surveillance and control of Chagas disease in Venezuela.

Chagas Disease (CD), a chronic infection caused by the Trypanosoma cruzi parasite, is a Neglected Tropical Disease endemic to Latin America. With a re-emergence in Venezuela during the past two decades, the spread of CD has proved susceptible to, and inhibitable by a digital, real-time surveillance system effectuated by Citizen Scientists in communities throughout the country. The #TraeTuChipo (#BringYourKissingBug) campaign implemented in January 2020, has served as such a strategy counting on community engagement to define the current ecological distribution of CD vectors despite the absence of a functional national surveillance program. This pilot campaign collected data through online surveys, social media platforms, and/or telephone text messages. A total of 79 triatomine bugs were reported from eighteen Venezuelan states; 67 bugs were identified as Panstrongylus geniculatus, 1 as Rhodnius pictipes, 1 as Triatoma dimidiata, and 10 as Triatoma maculata. We analyzed 8 triatomine feces samples spotted from 4 Panstrongylus geniculatus which were confirmed positive by qPCR for T. cruzi. Further molecular characterization of discrete typing units (DTUs), revealed that all samples contained TcI, the most highly diverse and broadly distributed strain of T. cruzi. Moreover, analysis of the mitochondrial 12S gene revealed Myotis keaysi, Homo sapiens, and Gallus gallus as the main triatomine feeding sources. This study highlights a novel Citizen Science approach which may help improve the surveillance systems for CD in endemic countries

Flavanols and Anthocyanins in Cardiovascular Health: A Review of Current Evidence

Nowadays it is accepted that natural flavonoids present in fruits and plant-derived-foods are relevant, not only for technological reasons and organoleptic properties, but also because of their potential health-promoting effects, as suggested by the available experimental and epidemiological evidence. The beneficial biological effects of these food bioactives may be driven by two of their characteristic properties: their affinity for proteins and their antioxidant activity. Over the last 15 years, numerous publications have demonstrated that besides their in vitro antioxidant capacity, certain phenolic compounds, such as anthocyanins, catechins, proanthocyanidins, and other non coloured flavonoids, may regulate different signaling pathways involved in cell survival, growth and differentiation. In this review we will update the knowledge on the cardiovascular effects of anthocyanins, catechins and proanthocyanidins, as implied by the in vitro and clinical studies on these compounds. We also review the available information on the structure, distribution and bioavailability of flavanols (monomeric catechins and proanthocyanidins) and anthocyanins, data necessary in order to understand their role in reducing risk factors and preventing cardiovascular health problems through different aspects of their bioefficacy on vascular parameters (platelet agregation, atherosclerosis, blood pressure, antioxidant status, inflammation-related markers, etc.), myocardial conditions, and whole-body metabolism (serum biochemistry, lipid profile), highlighting the need for better-designed clinical studies to improve the current knowledge on the potential health benefits of these flavonoids to cardiovascular and metabolic health

Global Variation of Nutritional Status in Children Undergoing Chronic Peritoneal Dialysis : A Longitudinal Study of the International Pediatric Peritoneal Dialysis Network

While children approaching end-stage kidney disease (ESKD) are considered at risk of uremic anorexia and underweight they are also exposed to the global obesity epidemic. We sought to investigate the variation of nutritional status in children undergoing chronic peritoneal dialysis (CPD) around the globe. The distribution and course of body mass index (BMI) standard deviation score over time was examined prospectively in 1001 children and adolescents from 35 countries starting CPD who were followed in the International Pediatric PD Network (IPPN) Registry. The overall prevalence of underweight, and overweight/obesity at start of CPD was 8.9% and 19.7%, respectively. Underweight was most prevalent in South and Southeast Asia (20%), Central Europe (16.7%) and Turkey (15.2%), whereas overweight and obesity were most common in the Middle East (40%) and the US (33%). BMI SDS at PD initiation was associated positively with current eGFR and gastrostomy feeding prior to PD start. Over the course of PD BMI SDS tended to increase on CPD in underweight and normal weight children, whereas it decreased in initially overweight patients. In infancy, mortality risk was amplified by obesity, whereas in older children mortality was markedly increased in association with underweight. Both underweight and overweight are prevalent in pediatric ESKD, with the prevalence varying across the globe. Late dialysis start is associated with underweight, while enteral feeding can lead to obesity. Nutritional abnormalities tend to attenuate with time on dialysis. Mortality risk appears increased with obesity in infants and with underweight in older children.Peer reviewe

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Elective cancer surgery in COVID-19-free surgical pathways during the SARS-CoV-2 pandemic: An international, multicenter, comparative cohort study

PURPOSE As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19–free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19–free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19–free surgical pathways. Patients who underwent surgery within COVID-19–free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19–free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score–matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19–free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION Within available resources, dedicated COVID-19–free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks