Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis

We provide a descriptive characterization of the unfolded protein response (UPR) in skeletal muscle of human patients with peritoneal sepsis and a sepsis model of C57BL/6J mice. Patients undergoing open surgery were included in a cross-sectional study and blood and skeletal muscle samples were taken. Key markers of the UPR and cluster of differentiation 68 (CD68) as surrogate of inflammatory injury were evaluated by real-time PCR and histochemical staining. CD68 mRNA increased with sepsis in skeletal muscle of patients and animals (p < 0.05). Mainly the inositol-requiring enzyme 1α branch of the UPR was upregulated as shown by elevated X-box binding-protein 1 (XBP1u) and its spliced isoform (XBP1s) mRNA (p < 0.05, respectively). Increased expression of Gadd34 indicated activation of PRKR-Like Endoplasmic Reticulum Kinase (PERK) branch of the UPR, and was only observed in mice (p < 0.001) but not human study subjects. Selected cell death signals were upregulated in human and murine muscle, demonstrated by increased bcl-2 associated X protein mRNA and TUNEL staining (p < 0.05). In conclusion we provide a first characterization of the UPR in skeletal muscle in human sepsis

Runoff extremity in the Upper Lužnice catchment area

Thesis subject is the evaluation of runoff and flood regime of rivers Lužnice and Skřemelice at the closing profiles just before their confluences. The results are compared with findings from the profile Pilař, which were published in the past by other authors. More attention is paid to the evaluation of the hydrological year 2013 and in detail is described the flood in June of the same year. Daily flow data from the years 1971 - 2014 were used for evaluating of runoff conditions. The assessment of the runoff regime in terms of daily, monthly and annual flows were compared with the runoff regime in Pilař gauge station. The source regions with dominating influence on the resulting runoff were discovered. Analysis of the flood regime confirmed that spring floods in Lužnice came mainly from upland and hilly parts of catchment and large summer floods have main source area in the catchment of Lužnice river itself, before the confluence of the Lužnice river and Skřemelic river. When assessing flood in 2013, the main source areas of flood flows that hit Lužnice river basin were founded. The flood extremity was compared in each closing profiles

International Urban Design Studio 2018, Kyojima-Sumida District, Tokyo

The Tokyo Smart City Studio is a practical capstone project housed within the Eco Urban Lab at Georgia Tech’s School of City and Regional Planning and School of Architecture. Throughout the four-month semester, students collaborate on innovative urban design solutions for some of Tokyo’s most important problems. In conjunction with the Global Carbon Project (GCP), the National Institute for Environmental Studies of Japan, the Department of Urban Engineering of the University of Tokyo, and the University of Tsukuba, Georgia Tech Students tackled issues ranging from energy consumption and disaster preparedness to heat stress and a vulnerable elderly population. The group completed five comprehensive reviews, a week-long site visit to Tokyo, multiple workshopping sessions, an Architecture Exposition, and two final reports. The focus area of this year’s studio was Kyojima, a one-half kilometer neighborhood in Sumida-Ku. In the late 19th century, this neighborhood was characterized by paddy fields, marshes, and a few small factories. It’s known for its traditional Japanese crafts, tight alleyways, and wooden tenement housing. The area is dense and in need of revitalization. Kirakira Street, the neighborhood’s once bustling shopping destination, is in substantial economic decline. This document is a detailed report of all student proposals aimed at assisting community members and other Kyojima stakeholders with technological, design, and policy solutions

Additional file 3: of Chemerin in peritoneal sepsis and its associations with glucose metabolism and prognosis: a translational cross-sectional study

Figure S2. Correlation of fasting and intra-operative glucose levels with circulating chemerin in controls. a Correlation of circulating chemerin levels with fasting glucose levels (r = 0.216, p = 0.034, n = 17). b Correlation of circulating chemerin levels with OP glucose (r = 0.549, p = 0.023, n = 17). (PDF 44 kb