Oral delivery system for tetanus toxoid.

Se ha estudiado la respuesta sistémica e inmune local frente al toxoide tetánico (TT) encapsulado en micropartículas de quitosano (CS), (CS-TT) preparado mediante reticulación iónica con tripolifosfato de sodio (STPP). Se administraron a ratones micropartículas CS-TT, TT en tampón de fosfato salino (PBS) y micropartículas CS simples, y se administró TT (adsorbido) por vía intramuscular. Se analizó el suero para determinar los niveles de IgG anti-TT y se realizó un lavado intestinal para determinar los niveles de IgA anti-TT mediante la técnica ELISA. El estudio demuestra la efi cacia del sistema de suspensión de micropartículas de quitosano, con TT, para inducir IgA en el intestino e IgC en la circulación sistémica.Systemic and local immune response against tetanus toxoid (TT) encapsulated in chitosan (CS) microparticles (CSTT) prepared by ionic cross-linking using Sodium Tripolyphosphate (STPP) was studied. CS-TT microparticles, TT in PBS and plain CS microparticles were orally administered to mice and TT (adsorbed) was administered through intramuscular route. Sera were analyzed for anti-TT IgG and intestinal lavage for anti-TT IgA levels using an ELISA. The study shows the effi cacy of chitosan microparticle suspension system, containing a TT, in inducing the IgA in intestine and IgG in systemic circulation

Global, regional, and national age-sex-specific mortality and life expectancy, 1950–2017: a systematic analysis for the Global Burden of Disease Study 2017

BACKGROUND: Assessments of age-specific mortality and life expectancy have been done by the UN Population Division, Department of Economics and Social Affairs (UNPOP), the United States Census Bureau, WHO, and as part of previous iterations of the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD). Previous iterations of the GBD used population estimates from UNPOP, which were not derived in a way that was internally consistent with the estimates of the numbers of deaths in the GBD. The present iteration of the GBD, GBD 2017, improves on previous assessments and provides timely estimates of the mortality experience of populations globally. METHODS: The GBD uses all available data to produce estimates of mortality rates between 1950 and 2017 for 23 age groups, both sexes, and 918 locations, including 195 countries and territories and subnational locations for 16 countries. Data used include vital registration systems, sample registration systems, household surveys (complete birth histories, summary birth histories, sibling histories), censuses (summary birth histories, household deaths), and Demographic Surveillance Sites. In total, this analysis used 8259 data sources. Estimates of the probability of death between birth and the age of 5 years and between ages 15 and 60 years are generated and then input into a model life table system to produce complete life tables for all locations and years. Fatal discontinuities and mortality due to HIV/AIDS are analysed separately and then incorporated into the estimation. We analyse the relationship between age-specific mortality and development status using the Socio-demographic Index, a composite measure based on fertility under the age of 25 years, education, and income. There are four main methodological improvements in GBD 2017 compared with GBD 2016: 622 additional data sources have been incorporated; new estimates of population, generated by the GBD study, are used; statistical methods used in different components of the analysis have been further standardised and improved; and the analysis has been extended backwards in time by two decades to start in 1950. FINDINGS: Globally, 18·7% (95% uncertainty interval 18·4–19·0) of deaths were registered in 1950 and that proportion has been steadily increasing since, with 58·8% (58·2–59·3) of all deaths being registered in 2015. At the global level, between 1950 and 2017, life expectancy increased from 48·1 years (46·5–49·6) to 70·5 years (70·1–70·8) for men and from 52·9 years (51·7–54·0) to 75·6 years (75·3–75·9) for women. Despite this overall progress, there remains substantial variation in life expectancy at birth in 2017, which ranges from 49·1 years (46·5–51·7) for men in the Central African Republic to 87·6 years (86·9–88·1) among women in Singapore. The greatest progress across age groups was for children younger than 5 years; under-5 mortality dropped from 216·0 deaths (196·3–238·1) per 1000 livebirths in 1950 to 38·9 deaths (35·6–42·83) per 1000 livebirths in 2017, with huge reductions across countries. Nevertheless, there were still 5·4 million (5·2–5·6) deaths among children younger than 5 years in the world in 2017. Progress has been less pronounced and more variable for adults, especially for adult males, who had stagnant or increasing mortality rates in several countries. The gap between male and female life expectancy between 1950 and 2017, while relatively stable at the global level, shows distinctive patterns across super-regions and has consistently been the largest in central Europe, eastern Europe, and central Asia, and smallest in south Asia. Performance was also variable across countries and time in observed mortality rates compared with those expected on the basis of development. INTERPRETATION: This analysis of age-sex-specific mortality shows that there are remarkably complex patterns in population mortality across countries. The findings of this study highlight global successes, such as the large decline in under-5 mortality, which reflects significant local, national, and global commitment and investment over several decades. However, they also bring attention to mortality patterns that are a cause for concern, particularly among adult men and, to a lesser extent, women, whose mortality rates have stagnated in many countries over the time period of this study, and in some cases are increasing

Mucoadhesive bilayer tablets for buccal delivery of carvedilol: in vitro and in vivo investigations.

Se diseñó un sistema mucoadherente bicapa de carvedilol para administración bucal, con el objetivo de mejorar la biodisponibilidad y conseguir una liberación sostenida. Como portadores se utilizaron Carbopol 934P e hidroxipropilmetilcelulosa K4M, y las formulaciones obtenidas se sometieron a estudios de pH superfi cial, índice de expansión y bioadhesión y liberación de fármaco in vitro. Se analizó la cinética de los datos de liberación de fármaco in vitro, mediante ajuste en modelos de orden cero, primer orden, Higuchi, Hixson-Crowell y Korsmeyer Peppas. El estudio del rendimiento farmacocinético in vivo del lote optimizado se realizó en conejos. Los datos obtenidos de la optimización y evaluación del sistema revelaron que las formulaciones presentaban índices de expansión y parámetros tecnológicos satisfactorios. La formulación F5 presentó el mayor grado de bioadherencia (3.5 ± 0.6 N) y su retención en la mucosa bucal porcina fue de 7 h. El ajuste al modelo de los datos de liberación in vitro demostró que seguían un patrón de liberación de orden cero con un comportamiento de liberación no Fickian, es decir, con valores de n entre 0.71 y 1.17, lo que indica que la liberación fue una combinación de erosión del comprimido y difusión desde la matriz. Los valores farmacocinéticos obtenidos presentaron una diferencia signifi cativa entre Cmax, Tmax y los valores del área bajo la curva de las formulaciones bucal y oral, es decir, un aumento de la biodisponibilidad en los comprimidos bucales en comparación con la formulación oral. Las curvas de concentración en plasma de los comprimidos bucales evidenciaron claramente un comportamiento de liberación sostenido.Mucoadhesive bilayered system of carvedilol was designed for buccal application with the objective of improving bioavailability and producing sustained release. Carbopol 934P and hydroxypropylmethylcellulose K4M were employed as carriers and the developed formulations were subjected for evaluation of surface pH, swelling index, in vitro bioadhesion and in vitro drug release studies. In vitro drug release data was analyzed for release kinetics by fi tting into Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer Peppas models. In vivo pharmacokinetic performance of the optimized batch was investigated in rabbits. Data emerged from optimization and evaluation of the system revealed that formulations exhibited satisfactory technological parameters and swelling indices. Formulation F5 showed maximum bioadhesion (3.5 ± 0.6 N) and was found to be retained for 7 h on porcine buccal mucosa. The model fi tting of in vitro release data demonstrated that it followed zero order release pattern with non-fi ckian release behavior i.e. n values ranging from 0.71 to 1.17, indicating the release was combination of tablet erosion and diffusion from the matrix. The obtained pharmacokinetic values showed signifi cant difference between Cmax, Tmax and area under curve values of oral and buccal formulation, i.e. increase in bioavailability of buccal tablet as compared with oral formulation. Plasma concentration curves for the buccal tablet clearly showed evidence of sustained release behavio

Not Available

Not AvailableTo evaluate the efficiency of different microalgae in larval rearing of Indian white shrimp, Penaeus indicus, three different microalgae: Tetraselmis sp., Chaetoceros gracilis and Thalassiosira weissflogii were fed to the shrimp larvae either individually or in combination of two (1:1). The experiment showed that the survival, growth and stage conversion rate were significantly (p <  0.05) higher in combination of T. weissflogii: Tetraselmis sp. followed by T. weissflogii: C. gracilis. Further, significantly faster conversion rate was found in the treatment fed with combination diet (T. weissflogii: Tetraselmis and T. weissflogii: C. gracilis). The growth kinetics of three algae revealed that T. weissflogii had better growth potential than other two algae. The nutrient profiles of three microalgae also underline the nutritional superiority of T. weissflogii in terms of lipid, protein and essential fatty acids (EFA) over the others. Moreover, T. weissflogii showed better antimicrobial properties compared to other algae.Not AvailableICA