Deciphering the H-Bonding Preference on Nucleoside Molecular Recognition through Model Copper(II) Compounds

The synthetic nucleoside acyclovir is considered an outstanding model of the natural nucleoside guanosine. With the purpose of deepening on the influence and nature of non-covalent interactions regarding molecular recognition patterns, three novel Cu(II) complexes, involving acyclovir (acv) and the ligand receptor N-(2-hydroxyethyl)ethylenediamine (hen), have been synthesized and thoroughly characterized. The three novel compounds introduce none, one or two acyclovir molecules, respectively. Molecular recognition has been evaluated using single crystal X-ray diffraction. Furthermore, theoretical calculations and other physical methods such as thermogravimetric analysis, infrared and UV-Vis spectroscopy, electron paramagnetic resonance and magnetic measurements have been used. Theoretical calculations are in line with experimental results, supporting the relevance of the [metal-N7(acv) + H-bond] molecular recognition pattern. It was also shown that (hen)O-H group is used as preferred H-donor when it is found within the basal coordination plane, since the higher polarity of the terminal (hen)O-H versus the N-H group favours its implication. Otherwise, when (hen)O-H occupies the distal coordination site, (hen)N-H groups can take overThis research was funded by Agencia Estatal de Investigación, Ministerio de Ciencia, Innovación y Universidades (MICIU) from Spain and co-funded with FEDER-EU (Projects No. PGC2018-102047-B-I00 and CTQ2017-85821-R); Junta de Andalucía (FQM-283), and University of Granada (Project ref. PPJIA2019-03)S

Non-lethal suicidal behavior in university students of Spain during COVID-19

BackgroundSuicide is the fourth external cause of death in the world, in persons between the ages of l5 and 29. The objectives of this study were to measure the prevalence of suicidal behavior in university students and analyze the relationship of suicide risk with psychological distress, resilience, and family and social support.MethodsAn observational and transversal study wherein the students at the University of Malaga (Spain) completed an online questionnaire which included items from different scales, sociodemographic and academic questions, and the subjective impact of the COVID-19 pandemic. Descriptive analyses and prevalence rates of suicidal behavior were calculated, and bivariate analyses, multiple linear regression, and a mediation and moderation analyses were conducted.ResultsA total of 2,212 students completed the questionnaire. The prevalence of the last 6 months was 30.4% wishing for death, 14.7% suicidal ideation, 5% self-harm injuries, and 0.5% suicide attempts. Psychological distress, family and social support were linked to the risk of suicide. Lastly, resilience and family support measure and moderate the relation between psychological stress and suicide risk.ConclusionPsychological distress is a risk factor for suicidal behavior, while resilience and family and social support are linked to a lower risk of suicide

Impact of Wnt/β-Catenin Inhibition on Cell Proliferation through CDC25A Downregulation in Soft Tissue Sarcomas.

The Wnt signaling pathway is an important cellular mechanism for regulating differentiation processes as well as cell cycle events, and different inhibitors of this pathway, for example, PRI-724, are showing promising results in clinical trials for treatment of advanced pancreatic adenocarcinoma or ovarian cancer. Growing evidence suggests that Wnt signaling may also be crucial for tumorigenesis and progression of soft tissue sarcomas (STS), a malignant neoplasm with few therapeutic options at an advanced state. Our study with several STS cell lines and primary cultures shows that inhibition of Wnt/β-catenin signaling with PRI-724 is able to suppress cell viability/proliferation and to increase cell death rates. TCF/β-catenin-mediated transcriptional activity is decreased in treated cells, leading to downregulation of its target genes CCND1 and CDC25A. The latter was critical because its downregulation via siRNA was able to mimic the effect of PRI-724 on cell cycle arrest and cell death induction. An evaluation of NCBI/GenBank data confirmed that CDC25A mRNA is elevated in STS patients. Importantly, PRI-724 in combination with standard STS chemotherapeutics doxorubicin or trabectedin enhanced their antitumoral effect in a synergistic manner according to isobolographic analysis, suggesting that Wnt inhibition through PRI-724 could be a beneficial combination regime in patients with advanced STS.This study was financed by Grupo Español de Investigación en Sarcomas (GEIS) and Fundación Mari PazJiménez Casado. MPC is supported by Programa Estrategia de Emprendimiento y Empleo Joven,Garantía Juvenil(Ministerio de Trabajo, Migraciones y Seguridad Social-SOIB.S

The case of the Itata estuary (Bio-Bio Region-Chile) plant formations: anthropogenic interference or natural disturbance-induced diversity enrichment?

The current study examined the relationship between native and introduced plant species, as indicators of the state of anthropogenic influence on plant formations in the area of the Itata estuary (Bío-Bío Region, Chile). A total of 186 vegetation samples were collected in different plant communities in the wetlands and adjacent areas of the Itata River during 2011 and 2012. The communities of four terrestrial plant formations were sampled within dunes, prairies, shrub lands and food crops, and within two aquatic habitats (freshwater and salt marsh). The total flora comprised of 222 species, these were dominated by introduced taxa with thirty-three species considered invasive for Chile. The high percentage contribution of these alien weeds to the total community could be interpreted as signs of a strong degree of anthropogenic interference in the natural plant formations. However, some habitats such as salt marshes are subjected to periodic natural disturbances (e.g. tsunamis), lacking human interference. Consequently, in some habitats, alien species, which are more resilient, represent the primary plant formations.</p

Recensiones [Revista de Historia Económica Año XIX Otoño-Invierno 2001 n. 3 pp. 707-751]

Editada en la Fundación Empresa PúblicaTortella, G. La revolución del siglo XX (Por Jordi Palafox Gámir).-- Marichal, C. La bancarrota del virreinato. Nueva España y las finanzas del imperio español, 1780-1810 (Por Pedro Pérez Herrero).-- Eicnengreen, B. La globalización del capital. Historia del Sistema Monetario Internacional (Por Ana Fernández-Ardavín).-- Hayes, P. Industry and Ideology. IG Farben in the Nazi Era (Por Nuria Puig Raposo).-- Sáez García, M. A. Álava en la siderurgia moderna española. San Pedro de Araya (1847-1935) (Por Stefan Houpt).-- Brincas Gutiérrez, M. A. La productividad de los factores en la agricultura española, 1752-1935 (Por Esteban Nicolini).-- Madrazo, S. Estado débil y ladrones poderosos en la España del siglo XVIII. Historia de un peculado en el reinado de Felipe V (Por Juan Zafra).-- Jurado Sánchez, J. El gasto de la Casa Real, su financiación y sus repercusiones hacendísticas y económicas (Por Luis María Bilbao).-- Ángulo Morales, A. Del éxito en los negocios al fracaso del Consulado. La formación de la burguesía mercantil de Vitoria (1670-1840) (Por Miguel Ángel Melón Jiménez).-- Marcos Martín, A. España en los siglos XVI,XVII y XVIII. Economía y Sociedad (Por Enrique Llopis Agelán).-- Tortella, T. Una guía de fuentes sobre inversiones extranjeras en España (1780-1914) (Por Pablo Martín Aceña)Publicad

Colocación de bioprótesis en terreno potencialmente contaminado en la intervención quirúrgica herniaria de urgencia

All patients undergoing emergency surgery due to hernia complications were studied. The universe and the sample consisted of two groups. The first group included all inguinal and femoral hernias and any combination of these. Inguinal hernias predominated in men older than 61 years. The most commonly used techniques were the Lichtenstein technique, in the prosthetic variants, and the Leo Zimmerman’s in the tension ones. Seroma was the most common complication of the above mentioned techniques and recurrence was higher in the tension ones – four cases, for a 4.5%. In the second group, all incisional and midline hernias were included (umbilical and epigastric). Most of the surgical interventions were umbilical hernias –69 patients– also in men older than 61 years. The technique described by Jean Rives was among the prosthetic variants, and the Mayo-Robson technique in the tension ones. Seroma was the most common complication in both techniques and recurrence was higher in the tension ones–five patients for 8.7%. The sample showed that the use of surgical mesh has not produced an increase in postoperative complications of septic origin but, on the contrary, there has been a considerable reduction of recurrence in emergency hernia surgery using bioprosthesis.Se estudiaron la totalidad de pacientes intervenidos de forma urgente por complicaciones herniarias. El universo y la muestra estuvieron conformados por dos grandes grupos: en el primero se incluyeron todas las hernias inguinales y crurales y cualquier combinación de estas; predominaron las hernias inguinales en hombres mayores de 61 años; las técnicas más usadas fueron la de Lichtenstein en las variantes protésicas y la de Leo Zimmerman en las tensionadas; el seroma fue la complicación común para las técnicas mencionadas y la recidiva fue mayor en las tensionadas -cuatro casos, para un 4.5%- y, en el segundo, se sumaron todas las hernias incisionales y las de la línea media (umbilicales y epigástricas); el mayor número de intervenciones quirúrgicas correspondió a las hernias umbilicales -69 pacientes-, también en hombres mayores de 61 años; la técnica descrita por Jean Rives fue una de las variantes protésicas, así como la de Mayo-Robson en las tensionadas; el seroma fue la complicación más frecuente en ambas técnicas y la recidiva marcó pauta en las tensionadas -cinco pacientes, para un 8.7%-. En la muestra se evidenció que el uso de las mallas quirúrgicas no ha producido un alza en las complicaciones post-operatorias de origen sépticas sino que, por el contrario, han disminuido considerablemente las recidivas en las intervenciones quirúrgicas de la hernia de urgencia con el uso de la bioprótesis

Interaction between ATM and PARP-1 in response to DNA damage and sensitization of ATM deficient cells through PARP inhibition

ATM and PARP-1 are two of the most important players in the cell's response to DNA damage. PARP-1 and ATM recognize and bound to both single and double strand DNA breaks in response to different triggers. Here we report that ATM and PARP-1 form a molecular complex in vivo in undamaged cells and this association increases after γ-irradiation. ATM is also modified by PARP-1 during DNA damage. We have also evaluated the impact of PARP-1 absence or inhibition on ATM-kinase activity and have found that while PARP-1 deficient cells display a defective ATM-kinase activity and reduced γ-H2AX foci formation in response to γ-irradiation, PARP inhibition on itself is able to activate ATM-kinase. PARP inhibition induced γ H2AX foci accumulation, in an ATM-dependent manner. Inhibition of PARP also induces DNA double strand breaks which were dependent on the presence of ATM. As consequence ATM deficient cells display an increased sensitivity to PARP inhibition. In summary our results show that while PARP-1 is needed in the response of ATM to gamma irradiation, the inhibition of PARP induces DNA double strand breaks (which are resolved in and ATM-dependent pathway) and activates ATM kinase

Guía Global de Practicum en Enfermería. Una necesidad para el aprendizaje y la evaluación

El alumnado acude a la Universidad con intención de adquirir competencias profesionales. Planteamos el currículo de titulación y su contenido y el método deben traducirse eficazmente en la práctica. Si encontramos un vacío provocamos un “shock de realidad.” Para evitarlo surge el consenso de Guía de Practicum con los Centros Sanitarios, la adquisición secuencial de competencias y la figura del Tutor Clínico coordinado con el profesorado. Este trabajo tiene como objetivo elaborar una Guía Académica Global del Practicum que fomente la participación activa entre los profesores UA, tutores clínicos y alumnado. Se ha realizado un análisis del contenido de 13 guías académicas. En el Practicum de cuarto han participado 41 profesores y existen 4 guías; en tercero han participado 31 profesores y existen 5 guías; en segundo 62 profesores con 4 guías académicas. Actualmente registrados en la aplicación informática y con asignación de estudiantes existen 1859 tutores clínicos en los distintos puestos de prácticas. El tipo de evaluación realizada es 100% continua. Como conclusión decir que, unificar criterios con el profesorado, formación de tutores, secuenciar objetivos por complejidad, seguimiento/evaluación de competencias coordinados UA/Empresas e Instituciones Sanitarias, investigación e innovación, son una necesidad para la calidad del proceso de enseñanza-aprendizaje

Fall prevention intervention technologies: A conceptual framework and survey of the state of the art

In recent years, an ever increasing range of technology-based applications have been developed with the goal of assisting in the delivery of more effective and efficient fall prevention interventions. Whilst there have been a number of studies that have surveyed technologies for a particular sub-domain of fall prevention, there is no existing research which surveys the full spectrum of falls prevention interventions and characterises the range of technologies that have augmented this landscape. This study presents a conceptual framework and survey of the state of the art of technology-based fall prevention systems which is derived from a systematic template analysis of studies presented in contemporary research literature. The framework proposes four broad categories of fall prevention intervention system: Pre-fall prevention; Post-fall prevention; Fall injury prevention; Cross-fall prevention. Other categories include, Application type, Technology deployment platform, Information sources, Deployment environment, User interface type, and Collaborative function. After presenting the conceptual framework, a detailed survey of the state of the art is presented as a function of the proposed framework. A number of research challenges emerge as a result of surveying the research literature, which include a need for: new systems that focus on overcoming extrinsic falls risk factors; systems that support the environmental risk assessment process; systems that enable patients and practitioners to develop more collaborative relationships and engage in shared decision making during falls risk assessment and prevention activities. In response to these challenges, recommendations and future research directions are proposed to overcome each respective challenge.The Royal Society, grant Ref: RG13082