Identification of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible genes in human amniotic epithelial cells

BACKGROUND: Exposure to dioxins results in a broad range of pathophysiological disorders in human fetuses. In order to evaluate the effects of dioxins on the feto-placental tissues, we analyzed the gene expression in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated primary cultures of human amniotic epithelial cells. METHODS: Human amniotic epithelial cells were dispersed by trypsin from amniotic membranes and cultured in DME/Ham's F12 medium supplemented with 10% FBS. Two weeks after plating, cells were treated with 50 nM TCDD or DMSO (control), further incubated for 48 hrs, and the gene expression was analyzed by DNA microarray technology and quantitative real-time PCR. RESULTS: Thirty eight TCDD-inducible genes, including cytochromeP4501A1 and cytochromeP4501B1, were identified. One of the remarkable profiles of the gene expression was the prominent up-regulation of interferon-inducible genes. The genes involved in the interferon gene expression and interferon signaling pathways were also up-regulated. Furthermore, the expression of genes related to collagen synthesis or degradation was enhanced by TCDD. CONCLUSION: Using DNA microarray and quantitative real-time PCR analyses, we identified TCDD-inducible genes, including interferon-inducible genes and genes related to collagen synthesis or degradation, in human amniotic epithelial cells

La5Ti2Cu1-xAgxS5O7 photocathodes operating at positive potentials during photoelectrochemical hydrogen evolution under irradiation of up to 710 nm

A photoelectrochemical (PEC) cell based on a series-connected photocathode and photoanode made of particulate semiconductors is a potentially scalable and inexpensive device for renewable solar hydrogen production via PEC water splitting without any external power supply. The realisation of such PEC devices hinges on the development of photoelectrodes that operate at a small applied voltage. In this study, solid solutions of La5Ti2CuS5O7 (LTC) and La5Ti2AgS5O7 (LTA) were synthesised, and their physical, optical, and PEC properties in the water splitting reaction were discussed. LTC and LTA formed a La5Ti2Cu1-xAgxS5O7 solid solution (LTC(1-x)A(x)) over the whole compositional range. The indirect bandgap energy of LTC(1-x)A(x) changed nonlinearly with respect to composition, attaining its minimum value (ca. 1.8 eV) at a composition of x approximate to 0.16. Photoelectrodes of Al-doped LTC(1-x)A(x) solid solution powder fabricated using the particle transfer method exhibited a photocathodic response regardless of the Ag content. 1% Al-LTC(0.9)A(0.1) photocathodes exhibited the best PEC properties in the hydrogen evolution reaction and yielded a hypothetical half-cell solar-to-hydrogen energy conversion efficiency of 0.25% at +0.6 V vs. RHE, three times higher than the previously reported 1% Sc-LTC. In addition, 1% Al-LTC(0.9)A(0.1) photocathodes were fairly stable at + 0.7 V vs. RHE without any protective modifications. Owing to the positive operational electrode potential of 1% Al-LTC(0.9)A(0.1), unassisted PEC water splitting was accomplished using series-connected photoelectrodes made of 1% Al-LTC(0.9)A(0.1) and BaTaO2N, particulate semiconductors with absorption edge wavelengths of 710 and 660 nm, respectively, at a Faradaic efficiency of unity and a solar-to-hydrogen energy conversion efficiency of approximately 0.1%.ArticleEnergy & Environmental Science.8(11):3354-3362(2015)journal articl

An Al-doped SrTiO3 photocatalyst maintaining sunlight-driven overall water splitting activity for over 1000 h of constant illumination

Photocatalytic water splitting is a viable approach to the large-scale production of renewable solar hydrogen. The apparent quantum yield for this reaction has been improved, but the lifespan of photocatalysts functioning under sunlight at ambient pressure have rarely been examined, despite the critical importance of this factor in practical applications. Herein, we show that Al-doped SrTiO3 (SrTiO3: Al) loaded with a RhCrOx (rhodium chromium oxide) cocatalyst splits water with an apparent quantum yield greater than 50% at 365 nm. Moreover, following the photodeposition of CoOOH and TiO2, this material maintains 80% of its initial activity and a solar-to-hydrogen energy conversion efficiency greater than or equal to 0.3% over a span of 1300 h under constant illumination by simulated sunlight at ambient pressure. This result is attributed to reduced dissolution of Cr in the cocatalyst following the oxidative photodeposition of CoOOH. The photodeposition of TiO2 further improves the durability of this photocatalyst. This work demonstrates a concept that could allow the design of longterm, large-scale photocatalyst systems for practical sunlight-driven water splitting.ArticleCHEMICAL SCIENCE.10(11):3196-3201(2019)journal articl

La5 Ti2 Cu0.9 Ag0.1 S5 O7 Modified with a Molecular Ni Catalyst for Photoelectrochemical H2 Generation.

The stable and efficient integration of molecular catalysts into p-type semiconductor materials is a contemporary challenge in photoelectrochemical fuel synthesis. Here, we report the combination of a phosphonated molecular Ni catalyst with a TiO2 -coated La5 Ti2 Cu0.9 Ag0.1 S5 O7 photocathode for visible light driven H2 production. This hybrid assembly provides a positive onset potential, large photocurrents, and high Faradaic yield for more than three hours. A decisive feature of the hybrid electrode is the TiO2 interlayer, which stabilizes the oxysulfide semiconductor and allows for robust attachment of the phosphonated molecular catalyst. This demonstration of an oxysulfide-molecular catalyst photocathode provides a novel platform for integrating molecular catalysts into photocathodes and the large photovoltage of the presented system makes it ideal for pairing with photoanodes.Japan Society for the Promotion of Science (JSPS); Christian Doppler Research Association (Austrian Federal Ministry of Science, Research and Economy and the National Foundation for Research, Technology and Development); OMV group; EPSRC, Grants-in-Aids for Scientific Research and for Young Scientists from JSP

Safety of Postoperative Administration of Human Urinary Trypsin Inhibitor in Lung Cancer Patients with Idiopathic Pulmonary Fibrosis

Patients with idiopathic pulmonary fibrosis (IPF) undergoing pulmonary resection for lung cancer carry risks of acute exacerbations of IPF (AE) postoperatively. Currently, agents which may attenuate AE are actively sought. Urinary trypsin inhibitor, ulinastatin, is a synthetic glycoprotein which may potentially inhibit various inflammatory factors associated with the development and progression of IPF. The present study was done to evaluate the effects of administration of high dose ulinastatin in lung cancer patients with IPF immediately following lung resection.Patients with IPFs radiologically diagnosed on high resolution CT, and histologically diagnosed resectable lung cancers, were eligible for the study. The effects of escalating doses of ulinastatin 3×10(5), 6×10(5), and 9×10(5) units/body/day, administered postoperatively for 3 days were evaluated. The endpoints were safety and feasibility.Nine patients were evaluated, in cohorts of 3 patients per dosage. Postoperative follow up ranged from 3 to 12 months (median 9 months). The postoperative courses were uneventful in all patients. No subjective adverse events such as abdominal symptoms or skin rashes, or objective adverse events as per serum laboratory tests, such as liver or kidney dysfunctions potentially attributable to ulinastatin administration were observed. AE was seen in one patient at 3 months after surgery, but since this occurred shortly after administration of chemotherapy, it was considered to be attributable to the chemotherapy rather than surgery.Ulinastatin administration after lung resection in lung cancer patients with IPF was considered to be safe and feasible. Further study is planned at the highest dose of this study to evaluate efficacy.UMIN.ac.jp/ctr/UMIN000002410

Rapid Accumulation of Polymorphonuclear Neutrophils in the Corpus luteum during Prostaglandin F2α-Induced Luteolysis in the Cow

Prostaglandin F2α (PGF2α) induces luteolysis within a few days in cows, and immune cells increase in number in the regressing corpus luteum (CL), implying that luteolysis is an inflammatory-like immune response. We investigated the rapid change in polymorphonuclear neutrophil (PMN) numbers in response to PGF2α administration as the first cells recruited to inflammatory sites, together with mRNA of interleukin-8 (IL-8: neutrophil chemoattractant) and P-selectin (leukocyte adhesion molecule) in the bovine CL. CLs were collected by ovariectomy at various times after PGF2α injection. The number of PMNs was increased at 5 min after PGF2α administration, whereas IL-8 and P-selectin mRNA increased at 30 min and 2 h, respectively. PGF2α directly stimulated P-selectin protein expression at 5–30 min in luteal endothelial cells (LECs). Moreover, PGF2α enhanced PMN adhesion to LECs, and this enhancement by PGF2α was inhibited by anti-P-selectin antibody, suggesting that P-selectin expression by PGF2α is crucial in PMN migration. In conclusion, PGF2α rapidly induces the accumulation of PMNs into the bovine CL at 5 min and enhances PMN adhesion via P-selectin expression in LECs. It is suggested that luteolytic cascade by PGF2α may involve an acute inflammatory-like response due to rapidly infiltrated PMNs

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements