PR-LncRNA Signature Regulates Glioma Cell Activity Through Expression of SOX Factors

Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.PA, JA-I and AS-A were recipients of a predoctoral fellowship from the Spanish Association Against Cancer (AECC Gipuzkoa), Basque Government and Instituto Salud Carlos III. This work was supported by grants from the Carlos III Institute of Health and the European Regional Development Fund (PI13/02277, CP16/00039, DTS16/084, and PI16/01580) and Industry and Health Departments of the Basque Country

Follow-up care over 12months of patients with prostate cancer in Spain. A multicenter prospective cohort study

This study was funded by an Instituto de Salud Carlos III (ISCIII) Grant PS09/01204 (Fondo de Investigación Sanitaria [FIS], Spain). Dr María José Martinez Zapata is funded by a Miguel Servet II research contract from the ISCIII (CP1120/00023). ISCIII had any role in the design or execution of the study; in the data collection, management, or interpretation; or in the writing, reviewing, or approval of the manuscript.Ailish Maher and Andrea Cervera Alepuz revised the English in a version of this manuscript. Maria José Martinez Zapata is funded by a Miguel Servet research contract (CPII20/00023). EMPARO Study Group: Coordinating investigator: Xavier Bonfill Cosp (Iberoamerican Cochrane Centre, Public Health and Clinical Epidemiology Service, Hospital de la Santa Creu i Sant Pau, IIB Sant Pau, Barcelona, Spain). Project manager: María José Martínez Zapata (Iberoamerican Cochrane Centre, IIBSant Pau, Barcelona, Spain). Clinical research assistants: Alborada Martínez (Universidad de Valencia); Enrique Morales Olivera (Escuela Andaluza de Salud Publica, Granada, Spain); Esther Canovas, Laura Muñoz, Gemma Mas, René Acosta, Ekaterina Popova (Iberoamerican Cochrane Centre, IIB Sant Pau, Barcelona, Spain); Irma Ospina (Hospital 12 de Octubre, Madrid, Spain); María José Velázquez (Hospital Donostia, Donostia, Spain); Tamara Ruiz Merlo (Hospital Ramón y Cajal, Madrid, Spain); Gael Combarros Herman, Judit Tirado Muñoz (IMIM-Hospital del Mar Medical Research Institute, Barcelona, Spain). Statistical analysis: Robin W.M. Vernooij (Iberoamerican Cochrane Centre, IIB Sant Pau, Barcelona, Spain); Javier Zamora and Claudia Coscia Requena (Hospital Ramón y Cajal, Madrid, Spain). Co-investigators: Barcelona, Spain Albert Frances (Hospital del Mar); Carola Orrego Villagran, Rosa Suñol (Instituto Universitario Avedis Donabedian); Dimelza Osorio, Gemma Sancho Pardo, Ignasi Bolívar, José Pablo Maroto, María Jesus Quintana, Cristina Martin (Hospital de la Santa Creu i Sant Pau); Ferran Algaba, Palou Redorta, Salvador Esquena (Fundació Puigvert); Jordi Bachs (Fundació Privada Hospital de la Santa Creu i Sant Pau); María José Martínez Zapata (Iberoamerican Cochrane Centre, IIB Sant Pau); Montserrat Ferrer Fores, Stefanie Schmidt, Olatz Garin, Virginia Becerra Bachito, Yolanda Pardo (IMIM-Hospital del Mar Medical Research Institute). Bilbao, Spain Amaia Martínez Galarza, José Ignacio Pijoán Zubizarreta (Hospital Universitario Cruces/BioCruces Health Research Institute). Granada, Spain Armando Suárez Pacheco, Cesar García López, José Manuel Cozar Olmo (Hospital Universitario Virgen de las Nieves); Carmen Martínez, Daysy Chang Chan, María José Sánchez Pérez (Escuela Andaluza de Salud Publica). Madrid, Spain Ana Isabel Díaz Moratinos, Angel Montero Luis, Asunción Hervás, Carmen Vallejo Ocaña, Costantino Varona, Javier Burgos, Javier Zamora, Jose Alfredo Polo Rubio, Luis López Fando Lavalle, Miguel Angel Jimenez Cidre, Muriel García, Alfonso, Nieves Plana Farras, Rosa Morera Lopez, Sonsoles Sancho Garcia, Victor Abraira, Victoria Gomez Dos Santos (Hospital Ramón y Cajal); Agustín Gómez de la Cámara, Javier de la Cruz, Juan Passas Martínez, Humberto García Muñoz, María Ángeles Cabeza Rodríguez (Hospital 12 de Octubre). San Sebastián, Spain Irune Ruiz Díaz, José Ignacio Emparanza, Juan Pablo Sanz Jaka (Hospital Universitario Donostia). Valencia, Spain Agustín Llopis González, María Morales (Universidad de Valencia); Carlos Camps, Cristina Caballero Díaz, Emilio Marqués Vidal, Francisco Sánchez Ballester, Joaquín Ulises Juan Escudero, Jorge Pastor Peidro, José López Torrecilla, María Macarena Ramos Campos, Miguel Martorell Cebollada (Consorcio Hospital General Universitario de Valencia).The therapeutic approach is crucial to prostate cancer prognosis. We describe treatments and outcomes for a Spanish cohort of patients with prostate cancer during the first 12 months after diagnosis and identify the factors that influenced the treatment they received. This multicenter prospective cohort study included patients with prostate cancer followed up for 12 months after diagnosis. Treatment was stratified by factors such as hospital, age group (<70 and ≥70 years), and D’Amico cancer risk classification. The outcomes were Eastern Cooperative Oncology Group (ECOG) performance status, adverse events (AEs), and mortality. The patient characteristics associated with the different treatment modalities were analyzed using multivariate logistic regression. We included 470 men from 7 Spanish tertiary hospitals (mean (standard deviation) age 67.8 (7.6) years), 373 (79.4%) of which received treatment (alone or in combination) as follows: surgery (n = 163; 34.7%); radiotherapy (RT) (n = 149; 31.7%); and hormone therapy (HT) (n = 142; 30.2%). The remaining patients (n = 97) were allocated to no treatment, that is, watchful waiting (14.0%) or active surveillance (5.7%). HT was the most frequently administered treatment during follow-up and RT plus HT was the most common therapeutic combination. Surgery was more frequent in patients aged <70, with lower histologic tumor grades, Gleason scores <7, and lower prostate-specific antigen levels; while RT was more frequent in patients aged ≥70 with histologic tumor grade 4, and higher ECOG scores. HT was more frequent in patients aged ≥70, with histologic tumor grades 3 to 4, Gleason score ≥8, ECOG ≥1, and higher prostate-specific antigen levels. The number of fully active patients (ECOG score 0) decreased significantly during follow-up, from 75.3% at diagnosis to 65.1% at 12 months (P < .001); 230 (48.9%) patients had at least 1 AE, and 12 (2.6%) patients died. Surgery or RT were the main curative options. A fifth of the patients received no treatment. Palliative HT was more frequently administered to older patients with higher tumor grades and higher Gleason scores. Close to half of the patients experienced an AE related to their treatment.Instituto de Salud Carlos III CP1120/00023, PS09/0120

Urban-rural interactions and their territorial disparities

Urban areas are usually considered the main centres of economic activity with a high population concentration, good infrastructure, service provision offering employment, social services, innovation and recreational opportunities that attract people to live there . However, cities are also heavily dependent on peri-urban and rural areas, especially for food production and basic products, management of natural resources, protection of natural landscape, as well as recreation and tourism. Mutual inter-dependencies exist over the continuum between urban, peri-urban and rural areas, conceptually described as ‘linkages’ or ‘flows’. These can be associated to people, goods and public services or environmental flows. Under the lens of the degree of urbanisation, this policy brief analyses territorial discrepancies and flows between types of settlements across several dimensions such accessibility of essential services and internet connection, housing market, tourism capacity, as well as natural resources and population dynamics. Incorporating this territorial perspective, strategic development policies have gained momentum with a tendency of creating large and strong functional regions with a focus on the enhancement and the intensification of urban-rural interactions that can help to reduce inequalities, ensure the well-being of citizens and the development of balanced and sustainable territories.JRC.B.3 - Territorial Developmen

The impact of COVID-19 on alternative and local food systems and the potential for the sustainability transition: Insights from 13 countries.

The COVID-19 pandemic has been a major stress test for the agri-food system. While most research has analysed the impact of the pandemic on mainstream food systems, this article examines how alternative and local food systems (ALFS) in 13 countries responded in the first months of the crisis. Using primary and secondary data and combining the Multi-Level Perspective with social innovation approaches, we highlight the innovations and adaptations that emerged in ALFS, and how these changes have created or supported the sustainability transition in production and consumption systems. In particular, we show how the combination of social and technological innovation, greater citizen involvement, and the increased interest of policy-makers and retailers have enabled ALFS to extend their scope and engage new actors in more sustainable practices. Finally, we make recommendations concerning how to support ALFS’ upscaling to embrace the opportunities arising from the crisis and strengthen the sustainability transition.EEA BalcarceFil: Nemes, Gusztáv. Centre for Economic and Regional Studies (KRTK); Hungría.Fil: Chiffoleau, Yuna. Institut National de la Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE); Francia.Fil: Zollet, Simona. Hiroshima University; Japón.Fil: Collison, Martin. Collison and Associates Limited; Estados Unidos.Fil: Benedek, Zsófia. Centre for Economic and Regional Studies, Hungría.Fil: Colantuono, Fedele. University of Foggia. Department of Economics; Italia.Fil: Dulsrud, Arne. SIFO Oslo Metropolitan University; Noruega.Fil: Fiore, Mariantonietta. University of Foggia. Department of Economics; Italia.Fil: Holtkamp, Carolin. University of Innsbruck. Department of Sociology; Austria.Fil: Tae-Yeon Kim. Dankook; Corea del Sur.Fil: Korzun, Monika. University of Guelph. School of Environmental Design and Rural Development; Canadá.Fil: Mesa-Manzano, Rafael. Universidad de Valencia. Instituto Interuniversitario de Desarrollo Local, España.Fil: Reckinger, Rachel. University of Luxembourg. Faculty of Humanities, Education and Social Sciences; Luxemburgo.Fil: Ruiz Martínez, Irune. Universidad de Valencia. Instituto Interuniversitario de Desarrollo Local, España.Fil: Smith, Kiah. The University of Queensland. School of Social Science; Australia.Fil: Tamura, Norie. Research Institute for Humanity and Nature; Japón.Fil: Viteri, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Viteri, María Laura. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias, Argentina.Fil: Orbán, Éva. Centre for Economic and Regional Studies; Hungría

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Internet Access in Rural Areas: Brake or Stimulus as Post-Covid-19 Opportunity?

The lack of internet access in most rural areas has become a challenge worldwide. The Covid-19 pandemic has highlighted trends such as teleworking and e-commerce, meaning an opportunity for the local economy of these areas, but with serious difficulties in carrying it out. This paper aims to detect this lack of internet in inland areas of the region of Valencia through local actors, in order to identify clear priorities and real needs through an explorative and replicable approach based on agglomerative hierarchical clustering (AHC). The main findings suggest that there are different patterns in the rural internet access related to adequate infrastructure and planned actions by local councils. In this way, a multitude of contextual elements have emerged that influence the importance of efficient access to the internet in rural areas. It is essential to know the real needs and demands of the population before implementing plans and programs that may not be relevant for the actors involved in territorial development.</jats:p

Psychosocial adjustment in adults with spina bifida: A systematic review of associated sociodemographic and clinical variables

The population of adults with SB represents a key clinical demographic demanding special attention. Owing to recent medical advancements, their increased life expectancy warrants a paramount focus on ensuring these added years are lived with QoL. Despite this progress, persistent evidence indicates that emotional and psychosocial difficulties (often enduring from childhood and adolescence) significantly impede their daily functioning and psychosocial adjustment. Given the critical need to identify the sociodemographic and clinical factors that modulate psychosocial adjustment in this population, a significant gap in the literature persists. To date, no comprehensive systematic review has thoroughly examined these specific factors in adults with SB. Therefore, the present study will conduct a systematic review of published research focusing on psychosocial adjustment in adults with SB. Gaining this comprehensive understanding will enable multidisciplinary healthcare teams to target specific needs, optimizing the detection and interprofessional management of key challenges. Ultimately, this improved focus will facilitate the development of targeted, interdisciplinary interventions with the potential to substantially elevate the psychosocial adjustment and overall QoL for adults with SB

The impact of COVID-19 on alternative and local food systems and the potential for the sustainability transition: Insights from 13 countries

The COVID-19 pandemic has been a major stress test for the agri-food system. While most research has analysed the impact of the pandemic on mainstream food systems, this article examines how alternative and local food systems (ALFS) in 13 countries responded in the first months of the crisis. Using primary and secondary data and combining the Multi-Level Perspective with social innovation approaches, we highlight the innovations and adaptations that emerged in ALFS, and how these changes have created or supported the sustainability transition in production and consumption systems. In particular, we show how the combination of social and technological innovation, greater citizen involvement, and the increased interest of policy-makers and retailers have enabled ALFS to extend their scope and engage new actors in more sustainable practices. Finally, we make recommendations concerning how to support ALFS’ upscaling to embrace the opportunities arising from the crisis and strengthen the sustainability transition