Detection and quantification of a mycorrhization helper bacterium and a mycorrhizal fungus in plant-soil microcosms at different levels of complexity

BACKGROUND: Host plant roots, mycorrhizal mycelium and microbes are important and potentially interacting factors shaping the performance of mycorrhization helper bacteria (MHB). We investigated the impact of a soil microbial community on the interaction between the extraradical mycelium of the ectomycorrhizal fungus Piloderma croceum and the MHB Streptomyces sp. AcH 505 in both the presence and the absence of pedunculate oak microcuttings. RESULTS: Specific primers were designed to target the internal transcribed spacer of the rDNA and an intergenic region between two protein encoding genes of P. croceum and the intergenic region between the gyrA and gyrB genes of AcH 505. These primers were used to perform real-time PCR with DNA extracted from soil samples. With a sensitivity of 10 genome copies and a linear range of 6 orders of magnitude, these real-time PCR assays enabled the quantification of purified DNA from P. croceum and AcH 505, respectively. In soil microcosms, the fungal PCR signal was not affected by AcH 505 in the absence of the host plant. However, the fungal signal became weaker in the presence of the plant. This decrease was only observed in microbial filtrate amended microcosms. In contrast, the PCR signal of AcH 505 increased in the presence of P. croceum. The increase was not significant in sterile microcosms that contained plant roots. CONCLUSIONS: Real-time quantitative PCR assays provide a method for directly detecting and quantifying MHB and mycorrhizal fungi in plant microcosms. Our study indicates that the presence of microorganisms and plant roots can both affect the nature of MHB-fungus interactions, and that mycorrhizal fungi may enhance MHB growth

12. Pääsuunnittelijakoulutuksen tutkielmat

Pääsuunnittelijakoulutus on suunnittelun ja johtamisen koulutusohjelma, jonka tavoitteena on tukea pääsuunnittelutehtävissä toimivien asiantuntijoiden edellytyksiä ja valmiuksia vastata tulevaisuuden osaamisvaatimuksiin. Julkaisu sisältää 12. pääsuunnittelijakoulutuksen tutkielmat: (1) Renni Haroma: Arkkitehdin vaikutusmahdollisuudet pääsuunnittelijana KVR-urakassa; tehtävät ja vastuut, sekä onnistuneen lopputuloksen varmistaminen (2) Pontus Högström: Rakennuttajan ja pääsuunnittelijan vastuut pienrakennushankkeessa (3) Outi Jääskeläinen: Pääsuunnittelija kunnallisessa rakennusinvestointihankkeessa (4) Tuomo Kallio: Tietomalli pääsuunnittelijan työkaluna - Muuttuvat ohjeet ja määräykset (5) Sami Karvinen: Rakennesuunnittelija pääsuunnittelijana - Tehtävän erityispiirteet (6) Pauli Koivisto: Pääsuunnittelijan tehtäviä korjausrakentamiskohteissa (7) Mika Kurth: Pääsuunnittelu tietomallihankkeessa - Pääsuunnittelijan, arkkitehdin ja tietomallikoordinaattorin roolit (8) Niklas Mahlberg: Kokemuksia yhteispohjoismaisesta rakennushankkeesta - Case: Teatteri- ja konserttitalo Kilden, Kristiansand, Norja (9) Joni Räisänen: Tietomallintaminen; haasteet ja mahdollisuudet - Tietomallintaminen pääsuunnittelijavetoisessa hankkeessa - Case: Alberga Business Park (10) Sebastian Rönnblad: Pääsuunnittelu tilaajan näkökulmasta (11) Esa Sallinen: Sosiaalinen toimilupa toimitilahankkeessa - vaikutukset pääsuunnittelijan rooliin (12) Kimmo Severinkangas: Pääsuunnittelijan ja rakentamiseen ryhtyvän tehtävä- ja vastuujako vesivoimalaitoksen rakennushankkeessa - Case: Suunnitteilla oleva Sierilän vesivoimalaitoshanke (13) Erki Valdre: Pääsuunnittelun riskienhallinta - Laatujärjestelmä riskienhallintavälineenä (14) Riitta Vesala: Verkostojohtamis- ja esimiestaidot pääsuunnittelijan tehtävässä

Metropolis versus necropolis: Polarity in the relationship between the city and the cemetery in history

The evolution of the city and the cemetery have always been running parallel in history, one couldn't exist without the other their relationship has always been strongly intertwined, yet strongly polarised. The aim of this report is to analyse key historical examples, relating to particular historical events, which in my opinion, may help to open - at least a partial view - on the evolution of the relationship between the city and the cemetery, affected by the shifting and changing balance of religious, political and socio-cultural components. In ancient times, the human body was taken as a reference to design the city first developing the metaphor of the city as a living organism - a metaphor which will be largely used through history, as will we see later. In the Greek and Roman cultures, the dead were not allowed to pollute the space of the living and the cemetery and the city were two separate entities. The advent of Christianity in Medieval times, saw the introduction of churchyards within the city walls, which also along with poor heath conditions of the then cities, facilitated the spread of epidemic diseases such as cholera. The Renaissance brought with it the desire to start afresh new cities and towns. The many projects of ideal cities left behind by architects such as Filarete or Scamozzi, were primarily concerned with the aesthetic and the security of the city rather than the real issues- health and hygiene and the spread epidemic diseases issues that finally urbanists had to address with the expansion of the cities due to the advent of the Industrial Revolution. Work forces during this particular historical moment moved from the countryside to city centres. The absence of any burial regulation and health issues urged the introduction of legislations which moved the cemeteries out of the city. In England, the Burial Act (which came to force in 1851) marked the introduction of suburban necropolises in the Victorian era, to resolve the issues of crowed inner city burial grounds, and guarantee city inhabitants a healthier life in the city centres. This also facilitated the condition for the development of a new strong architectural language specific to the cemetery in the Victorian era, which mostly came from cultures with strong past records of funerary traditions, like the Egyptians or the Romans. The new suburban necropolis in Victorian England, was the starting point of a new type of cemetery, which was also perceived as a public civic space, like a park. From now on we will see that the city and the cemetery will be almost completely separated: the city - growing into the metropolis, a complex, sometimes chaotic large urban development, and the cemetery - The Renaissance brought with it the desire to start afresh new cities and towns. The many projects of ideal cities left behind by architects such as Filarete or Scamozzi, were primarily concerned with the aesthetic and the security of the city rather than the real issues health and hygiene and the spread epidemic diseases issues that finally urbanists had to address with the expansion of the cities due to the advent of the Industrial Revolution. Work forces during this particular historical moment moved from the countryside to city centres. The absence of any burial regulation and health issues urged the introduction of legislations which moved the cemeteries out of the city. In England, the Burial Act (which came to force in 1851) marked the introduction of suburban necropolises in the Victorian era, to resolve the issues of crowed inner city burial grounds, and guarantee city inhabitants a healthier life in the city centres. This also facilitated the condition for the development of a new strong architectural language specific to the cemetery in the Victorian era, which mostly came from cultures with strong past records of funerary traditions, like the Egyptians or the Romans. The new suburban necropolis in Victorian England, was the starting point of a new type of cemetery, which was also perceived as a public civic space, like a park. From now on we will see that the city and the cemetery will be almost completely separated: the city - growing into the metropolis, a complex, sometimes chaotic large urban development, and the cemetery

Enceladus and Titan: Emerging Worlds of the Solar System (ESA Voyage 2050 White Paper)

Some of the major discoveries of the recent Cassini-Huygens mission have put Titan and Enceladus firmly on the Solar System map. The mission has revolutionised our view of Solar System satellites, arguably matching their scientific importance with that of their planet. While Cassini-Huygens has made big surprises in revealing Titan's organically rich environment and Enceladus' cryovolcanism, the mission's success naturally leads us to further probe these findings. We advocate the acknowledgement of Titan and Enceladus science as highly relevant to ESA's long-term roadmap, as logical follow-on to Cassini-Huygens. In this white paper, we will outline important science questions regarding these satellites and identify the pertinent science themes we recommend ESA cover during the Voyage 2050 planning cycle. Addressing these science themes would make major advancements to the present knowledge we have about the Solar System, its formation, evolution and likelihood that other habitable environments exist outside the Earth's biosphere

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Genome-wide analysis of 102,084 migraine cases identifies 123 risk loci and subtype-specific risk alleles

Migraine affects over a billion individuals worldwide but its genetic underpinning remains largely unknown. Here, we performed a genome-wide association study of 102,084 migraine cases and 771,257 controls and identified 123 loci, of which 86 are previously unknown. These loci provide an opportunity to evaluate shared and distinct genetic components in the two main migraine subtypes: migraine with aura and migraine without aura. Stratification of the risk loci using 29,679 cases with subtype information indicated three risk variants that seem specific for migraine with aura (in HMOX2, CACNA1A and MPPED2), two that seem specific for migraine without aura (near SPINK2 and near FECH) and nine that increase susceptibility for migraine regardless of subtype. The new risk loci include genes encoding recent migraine-specific drug targets, namely calcitonin gene-related peptide (CALCA/CALCB) and serotonin 1F receptor (HTR1F). Overall, genomic annotations among migraine-associated variants were enriched in both vascular and central nervous system tissue/cell types, supporting unequivocally that neurovascular mechanisms underlie migraine pathophysiology.publishedVersionPeer reviewe

Genome-wide analysis of 102,084 migraine cases identifies 123 risk loci and subtype-specific risk alleles.

To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked DownloadMigraine affects over a billion individuals worldwide but its genetic underpinning remains largely unknown. Here, we performed a genome-wide association study of 102,084 migraine cases and 771,257 controls and identified 123 loci, of which 86 are previously unknown. These loci provide an opportunity to evaluate shared and distinct genetic components in the two main migraine subtypes: migraine with aura and migraine without aura. Stratification of the risk loci using 29,679 cases with subtype information indicated three risk variants that seem specific for migraine with aura (in HMOX2, CACNA1A and MPPED2), two that seem specific for migraine without aura (near SPINK2 and near FECH) and nine that increase susceptibility for migraine regardless of subtype. The new risk loci include genes encoding recent migraine-specific drug targets, namely calcitonin gene-related peptide (CALCA/CALCB) and serotonin 1F receptor (HTR1F). Overall, genomic annotations among migraine-associated variants were enriched in both vascular and central nervous system tissue/cell types, supporting unequivocally that neurovascular mechanisms underlie migraine pathophysiology.US National Institute of Neurological Disorders and Stroke (NINDS) of the US National Institutes of Health (NIH) Finnish innovation fund Sitra Finska Lakaresallskapet Academy of Finland Sigrid Juselius Foundation Academy of Finland Appeared in source as:Academy of Finland Center of Excellence in Complex Disease Genetics Finnish Foundation for Cardiovascular Research Novo Nordisk Foundation Novocure Limited CANDY foundation (CEHEAD) South-Eastern Norway Regional Health Authorit