Rendimiento y calidad fisiológica de la semilla de Brachiaria spp. en los llanos colombianos

186 accesiones de 9 especies de Brachiaria, se establecieron en un diseño de franjas divididas, con 2 repeticiones, para evaluar 2 niveles de fertilidad: a) el recomendado para establecer pasturas; b) el nivel para establecer un sistema integrado arroz-pastos. En el tercer año se evaluó el potencial de rendimiento de semillas. En las pruebas de germinación y latencia se evaluó el efecto de 2 tratamientos: 1) Agua y 2) Semilla escarificada en ácido sulfúrico; las de b. humidicola se trataron con Nitrato de Potasio. El rendimiento promedio y el Peso Unidad de las semillas no presentaron diferencias significativas (P186 accessions of 9 species of Brachiaria were established in a design of fringes divided on 2 repetitions. In the third year seeds were evaluated to test their potencial under 2 different fertility levels: a) One recommended in order to establish pastures; b) Other in order to establish a system composed rice-pasture. The yield and the seed weight unit did not present significant differences (

Combining ability among apomictic and sexual parents of Urochloa humidicola.

The diallel cross constitutes an informative genetic design for choosing genitors and crosses in breeding programmes since it provides estimates of general (GCA) and specific (SCA) combining abilities. Because the breeding programme for Urochloa humidicola ([Rendle] Morrone & Zuloaga; syn. Brachiaria humidicola [Rendle] Schweick) at Embrapa Beef Cattle is recent, these estimates are still unknown. Thus, the aim of this study was to obtain the estimates of GCA and SCA from the partial diallel cross design between sexual and apomictic parents. The crosses involved nine sexual and ten apomictic parents. Seventy‐one full‐sib progenies were obtained and evaluated in an incomplete block design, using all parents and the cv. BRS Tupi as a check. The agronomic and forage quality traits were evaluated using seven harvests. Statistical analysis was performed using the mixed model approach. Significant variations were associated with GCA among sexual parents for biomass yield and forage quality traits. However, this cannot be observed for apomictic parents for GCA. The SCA effect was not significant for the assessed traits. These results showed the predominance of additive effects. Sexual parents SEX3 and SEX4 presented the highest potential for contributing favourable alleles in hybrid combinations. The absence of GCA among apomictic parents and SCA suggests the need to increase genetic variability for the generation of breeding populations of U. humidicola

Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points